Methods and compositions for translational profiling and molecular phenotyping

ABSTRACT

Methods and compositions are provided for translational profiling and molecular phenotyping of specific tissues, cells and cell subtypes of interest. The methods provided herein facilitate the analysis of gene expression in the selected subset present within a heterogeneous sample.

CROSS-REFERENCE

This application is a divisional of U.S. application Ser. No. 14/553,937, filed Nov. 25, 2014, and issued as U.S. Pat. No. 9,816,096 on Nov. 14, 2017, which is a continuation of U.S. application Ser. No. 12/918,761, filed Nov. 15, 2010, which is a national stage entry of International Application No. PCT/US09/01614, filed Mar. 12, 2009, which claims the benefit of U.S. Provisional Application Nos. 61/036,049 filed Mar. 12, 2008, 61/036,058 filed Mar. 12, 2008, 61/070,327, filed Mar. 21, 2008, and 61/199,108 filed Nov. 12, 2008, each of which applications is hereby incorporated by reference in its entirety.

STATEMENT AS TO FEDERALLY SPONSORED RESEARCH

This invention was made with the support of the U.S. Government under Grant No: AG09464 awarded by the National Institute on Aging, MH074866 awarded by the National Institute of Mental Health, DA10044 and 5F32DA021487 awarded by the National Institute on Drug Abuse, 5UL1RR024143 awarded by the National Institutes of Health/National Center for Research Resources, and NS34696 awarded by the National Institute of Neurological Disorders and Stroke. The U.S. Government may have certain rights to the subject matter provided herein.

SEQUENCE LISTING

In accordance with 37 CFR 1.52(e)(5), a Sequence Listing in the form of a text file (entitled “2011060-0012_Sequence_Listing”, created on Jan. 9, 2019, and having a size of 9,250 bytes) is hereby incorporated by reference in its entirety.

BACKGROUND OF THE INVENTION

A paradigm in the development of new diagnostics and therapies for human diseases and disorders is the characterization of the gene expression of defined cell types. The cellular complexity of many tissues poses a challenge for those seeking to characterize gene expression at this level. The enormous heterogeneity of a tissue such as the nervous system (thousands of neuronal cell types, with non-neuronal cells outnumbering neuronal cells by an order of magnitude) is a barrier to the identification and analysis of gene transcripts present in individual cell types. Cellular subtypes are highly heterogeneous and often intermixed. Gene expression studies on isolated cells have been limited by stresses introduced during cellular isolation procedures, the adaptations which occur upon the loss of tissue-intrinsic signals that control cellular physiology in vivo, and the technical challenges associated with reproducible mRNA purification from fixed tissue. A method to isolate translated mRNAs from defined cell types and subtypes without the need for cell isolation is needed in order to define previously undefined cell types, identify molecular targets for diseases and disorders, and provide a way in which to identify co-regulated gene sets for a particular biological function. The invention is described here.

INCORPORATION BY REFERENCE

All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference.

SUMMARY OF THE INVENTION

The invention described herein provides methods, compositions, and kits useful in elucidating the biological properties of distinct cellular populations. The embodiments described herein comprise a translating ribosome affinity purification (TRAP) methodology, which is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, environmental, pharmacological, other perturbations.

In one aspect, the invention described herein provides a method of identifying a co-regulated gene set for a function, comprising determining translational profiles for a plurality of cell types that express a gene associated with that function, comparing the translational profiles to determine what additional genes are similarly regulated, thereby identifying a co-regulated gene set involved in that function specifically. In one embodiment, the method is provided to identify a co-regulated gene set involved in myelination, and in a further embodiment the gene associated with myelination is Mbp. In one embodiment, the co-regulated gene set comprises at least two genes listed in Table 9. In a related embodiment, the gene set comprises at least two genes selected from the group consisting of Plp1, Cnp, Mog, Mal and Mobp.

In another aspect, the invention described herein provides a method for detecting one or more cell type-enriched genes not detectable by the whole-tissue microarray technique comprising assessing a translational profile for a cell type, comparing the translational profile to the results of a whole-tissue microarray, and determining the presence of one or more genes in the translational profile that are not present in the results of a whole-tissue microarray, thereby detecting one or more cell type-enriched genes that are not detectable by the whole-tissue microarray technique. In particular embodiments, greater than 20%, 30% or 40% of the genes enriched in that cell type are not detectable by the whole-tissue microarray technique. These methods can be applied to any cell type of interest including neuronal and non-neuronal cell types. In exemplary embodiments, the cell type is selected from the group consisting of striatal cell, cerebellar cell, cortical cell, hypothalamic cell, hippocampal cell, brainstem cell, and a spinal cord cell.

In another aspect, the invention described herein provides a method of identifying medium spiny neuron-enriched mRNAs, the method comprising expressing a ribosomal protein regulated by a regulatory region specific to a gene expressed in medium spiny neurons in an organism, isolating complexes comprising the ribosomal protein associated with mRNAs, identifying the mRNAs in the complexes, and comparing the mRNAs to those identified from a reference sample. In one embodiment the regulatory region used for expression is specific to a gene expressed in striatonigral neurons or striatopallidal neurons. In some embodiments the medium spiny neuron is associated with a disease or disorder such as but not limited to Parkinson's disease, addiction, attention deficit hyperactivity disorder, or Huntington's disease. In an additional embodiment, the method can be used in guiding the selection of candidate targets for the treatment of the disease or disorder, and for further screening of potential modulators of the candidate targets.

In yet another aspect, the invention described herein provides a method of identifying motor neuron-enriched mRNAs, the method comprising expressing a ribosomal protein regulated by a regulatory region specific to a gene expressed in motor neurons, isolating complexes comprising the ribosomal protein associated with mRNAs, identifying the mRNAs in the complexes, and comparing the mRNAs to those identified from a reference sample. In a specific embodiment, the regulatory region comprises regulatory sequences from a choline acetyltransferase (Chat) locus. This method can be utilized to identify mRNAS from any motor neuron, such as but not limited to brain stem motor neurons, spinal cord motor neurons, upper motor neurons and others.

In another aspect, the invention described herein provides a method for assessing the translational profile of a cerebellar cell, comprising expressing a ribosomal protein regulated by a regulatory region specific to a cerebellar gene in the cell, isolating at least one complex comprising the ribosomal protein associated with a mRNA from the cell, and identifying the mRNA from the complex whereby generating a translational profile. In one embodiment, the cerebellar cell is associated with a disease or disorder, such as ataxia.

The present invention also provides for kits comprising a recombinant vector engineered to express a nucleic acid sequence encoding a ribosomal protein and a detectable tag operably linked to an endogenous regulatory region, useful for practicing the methods described herein.

BRIEF DESCRIPTION OF THE DRAWINGS

The novel features of the invention are set forth with particularity in the appended claims A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings of which:

FIG. 1 illustrates a CNS disease-related BACarray collection.

FIG. 2 presents the translating ribosome affinity purification (TRAP) methodology. (a) Schematic of affinity purification of eGFP tagged polysomes (originating from the target cell population) using anti GFP antibody coated beads. (b) Transmission electron micrographs of anti GFP coated magnetic beads after incubation with extracts taken from HEK293T cells transfected with an empty vector (left panel) or the eGFP L10a construct (right panel); images acquired at 50,000× magnification, inserts enlarged by a factor of 2.3×.

FIG. 3 illustrates immunoprecipitation of translated mRNAs from transfected cells. FIG. 3 is comprised of Panels a, b, c, d, e, and f Panel a of FIG. 3 shows that induction of the iron-storage protein Ferritin was seen after 36 hours of iron treatment. Panel b of FIG. 3 shows post-mitochondrial supernatants of untreated or iron-treated cells loaded onto linear sucrose gradients (20-50% w/w). After velocity sedimentation, fractions (direction of sedimentation noted by arrow) were collected while UV absorbance (254 nm) was being measured. A representative trace is shown, as profiles from non-treated and iron-treated lysates looked nearly identical. Non-polysome and polysome gradient fractions were generated as indicated. To avoid Ferritin mRNAs associated with mRNPs, only heavier polysomes (with greater than 4 ribosomes) were included in the polysome fraction. Panel c of FIG. 3 shows that after iron treatment, as expected, a shift of Ferritin heavy chain mRNA (Fth1) out of non-polysome fractions into polysome fractions was observed, as determined by reverse transcription followed by quantitative PCR of total RNA purified from non-polysome and polysome gradient fractions (range of fold-change in non-polysome fraction: 0.16-0.22; range of fold-change in polysome fraction: 1.83-2.15). Panel d of FIG. 3 shows immunoprecipitations performed from non-polysome or polysome gradient fractions and 0.2% of the Input (IN), 0.02% of the unbound (UB); 1% of the bound (IP) samples were loaded onto gels for immunoblot analysis with eGFP or Rpl7 antibodies; * indicates the presence of a weak Rpl7 band upon much longer exposure. Rpl7 was not present in the non-polysome fraction, presumably because, unlike the overexpressed eGFP-Rpl10a, it was all incorporated into polysomes. As expected from the lack of Rpl7 (and thus assembled ribosomes) in the non-polysome fraction, immunoprecipitations from non-polysome fractions did not pull down any RNA above background, indicating that the immunoprecipitation was specific to translated messages. Panel e of FIG. 3 shows a direct eGFP immunoprecipitation of post-mitochondrial supernatants (whole cell lysates, unfractionated) performed to determine if the translating ribosome affinity purification (TRAP) methodology could faithfully reflect the changes observed in Panel c of FIG. 3. 0.5% of input (IN), 0.5% of the unbound fraction (UB), and 1.0% of the bound (IP) samples were loaded onto gels for immunoblot analysis with eGFP (top pane of Panel e of FIG. 3), Rpl10a (middle pane of Panel e of FIG. 3), or Rpl7 (bottom pane of Panel e of FIG. 3) antibodies. * indicates the presence of a light eGFP-L10a band upon longer exposure. No endogenous Rpl10a or Rpl7 was recovered in the bound (IP) fraction of mock samples, while endogenous Rpl10a and Rpl7 were both recovered in the bound (IP) fraction of untreated and iron-treated samples. Panel f of FIG. 3 shows the iron-induced fold-change in Fth1 mRNA levels relative to Actb mRNA levels in samples immunoprecipitated (IP) from either polysomes or from whole cell lysates (range of fold-change in polysome IP samples: 2.01-2.43; range of fold-change in direct IP samples: 1.80-1.93) was similar to the change observed in the polysome gradient fraction before immunoprecipitation (c).

FIG. 4 illustrates the in vivo BACarray strategy.

FIG. 5 illustrates BAC vector engineering.

FIG. 6 illustrates BACarray mouse generation.

FIG. 7 illustrates examples of drivers used to produce BACarray mice with specific expression in the cerebral cortex.

FIG. 8 illustrates examples of drivers used to produce BACarray mice with specific expression in the hypothalamus

FIG. 9 Panels 1-25 illustrates that BAC transgenesis targets eGFP-L10a to specific CNS cell populations. DAB immunohistochemistry with anti-eGFP antibody on each mouse line reveals a unique and specific pattern of expression for the eGFP-L10a transgene. Panels (10×) show the morphology and localization of cell types expressing the transgene, while inset shows location of panel, for cerebellar (1-9), spinal cord (10), striatal/basal forebrain (11-14) brainstem (15), and cortical (16-25) cell types. Dashed lines (panels 16-25) indicate corpus callosum. A key for all cell types is in FIG. 11A.

FIG. 10 illustrates that GENSTAT eGFP and BACarray cortical pyramidal cell lines have same laminar distribution. A) Graph (mean+/−SE) of the distance of eGFP+ cell somas from the pial surface for the BACarray and GENSAT eGFP lines for each pyramidal cell BAC driver. The depth of the cells was consistent between both lines for each driver. B) The percentage of cells in 100 micron bins is shown as a histogram of the distribution of cell depths for each line in A. The BACarray line and eGFP line for each driver had overlapping distributions of cell depths.

FIG. 11 illustrates a summary of cell types studied and characterization of lines. A) Table of all cell populations studied with BACarray, corresponding to FIG. 9. B) Detection of eGFP-L10a under the control of a Pcp2 BAC in Calbindin positive Purkinje cells. C) Detection of eGFP-L10a under the control of a Neurod1 BAC in granule cells with NeuN positive nuclei. D) Detection of eGFPL10a under the control of a Lypd6 BAC in Parvalbumin positive outer stellate and deep stellate (basket) neurons of the molecular layer, but not in Parvalbumin positive Purkinje cell fibers (arrow). E) Detection of eGFP-L10a under the control of a Grm2 BAC in Grm2/3 positive interneurons (Golgi cells) of the granule cell layer, but not in Grm2/3 positive glomeruli (arrow). F) eGFP-L10a detected under the control of a Grp BAC in unipolar brush cells with Grm1 positive brushes (arrow). G) eGFP-L10a detected under the control of a Sept4 BAC in s100 positive Bergman glia.

FIG. 12 illustrates that higher transgene expression yields better signal to noise. A) DAB immunohistochemistry for eGFP-L10a reveals differential intensity of transgene expression in two BACarray lines targeting the same cell population. B) Quality of BACarray data, as assessed by average IP/UB fold change for positive and negative controls, is better for the line with higher transgene expression.

FIG. 13 illustrates protein and mRNA purification from BACarray lines. (a) Purification of eGFP tagged L10a and co purification of untagged ribosomal protein L7 from D1 BACarray animals but not wild type littermates (D1, samples from D1 BACarray mice; WT, samples from wild type littermates; In, 1% Input; UB, 1% Unbound; IP, 6.5% Immunoaffinity purified sample). eGFP L10a signal is only present in the D1 IP lane because the IP samples were more concentrated relative to In and UB. (b) Purification of 18 S and 28 S rRNA from D1 BACarray transgenic animals (top panel) but not wild type littermates (bottom panel) as detected by Bioanalyzer PicoChips (Agilent Technologies). 28 S rRNA runs at ˜47 sec, 18 S rRNA runs at ˜43 sec, and the marker peak runs at ˜23 sec.

FIG. 14 illustrates electron microscopy of immunoprecipitated ribosome complexes.

FIG. 15 illustrates that microarray signal intensity does not increase with transcript length. A-DD: To assess whether longer mRNA's were more efficiently immunoprecipitated than shorter transcripts, transcript length (Y-axis, log base 2), was plotted versus GCRMA normalized signal intensity (X-axis, log base 2) for all 30 samples. None of the immunoprecipitated nor the unbound (whole tissue RNA) samples show positive correlations between length and signal.

FIG. 16 illustrates that BACarray can consistently purify RNA efficiently from rare and common cell types. A) Western blot for eGFP from S20 fraction, flow through (UB), and immunoprecipitate from rare (unipolar brush cells), common (Purkinje cells) and extremely common (granule cells) cell types of cerebellum. Note that even when protein is undetectable, there is sufficient good quality RNA for microarray amplification. B) Yield of RNA is consistent across replicates within each line. C) Total RNA is of good quality for all three cell types, with intact 18s and 28s bands, as well as mRNA which can be amplified and fragmented following standard protocols.

FIG. 17 illustrates that BACarray data are reproducible and cell-type specific. FIG. 17 is comprised of Panels A, B, C, and D. Panel A of FIG. 17 shows that replicates for the same cell type gave nearly identical genome wide translational profiles, confirming the results of Heiman et al, and extending this finding to many other cell types. The average Pearson's correlation between replicates for a given cell population from independently isolated samples was above 0.98 across all cell types. Panel B of FIG. 17 shows scatter plots for three representative cell types of the cerebellum. Panel C of FIG. 17 shows Venn diagrams constructed from the top 1000 most enriched probesets for each cell type. Panel D of FIG. 17 presents an examination of results obtained from independent BACarray founder lines prepared with the same engineered BAC to determine whether the position of integration of the BACarray construct would influence the data.

FIG. 18 illustrates that BACarrays can identify known markers, and discover new ones for a variety of cell types. FIG. 18 is comprised of Panels A, B, C, and D. Panel A of FIG. 18 shows a scatter plot of IP vs. UB for spinal cord motor neurons. Probesets for known markers of motor neurons with measurable signal on the array are clearly enriched in the IP sample, whereas probesets for glial cell-specific RNAs, that should not be present in these cells, are enriched in the UB sample. To establish the generality of this finding, the enrichment in the IP or UB sample was quantified by calculating an average ratio of IP/UB for positive and negative controls for each cell type where at least three positive controls could be found in the literature. Panel B of FIG. 18 shows that all IPs showed a clear enrichment for appropriate known markers (plotted in log base 2). Even for cell types with only one known marker (Pnoc positive interneurons, and Grp expressing unipolar brush cells), probesets for these genes were consistently and highly enriched in the IP. In the IPs with the lowest relative yield of RNA, such as those for mature oligodendrocytes (Panel B of FIG. 18), and Cort expressing interneurons, background was proportionally higher, and enrichment was less robust. Panel C of FIG. 18 shows that in the case of cerebellar Golgi cells, there is a great deal of overlap between eGFP-L10a expression in the BACarray line and expression of the genes chosen for this analysis. Panel D of FIG. 18 shows results of quantitative real time PCR (qRT-PCR) measuring the enrichment of a variety of mRNAs isolated from the Chat (motor neuron) and Pcp2 (Purkinje cell) BACarray transgenic lines in order to further validate the BACarray datasets. For all of the control genes tested, this methodology confirmed the BACarray results. For genes not previously known to be expressed in a specific cell type, results from qRTPCR demonstrated that seven out of the eight mRNAs assayed were in fact cell type enriched (Panel D of FIG. 18). Moreover, despite a negative ISH result, qRT-PCR validated the expression of Ceacam10 in the cerebellum and its enrichment in Golgi cells (Panel D of FIG. 18). In some cases, therefore, the translating ribosome affinity purification (TRAP) methodology appears to be more sensitive than ISH.

FIG. 19 illustrates that BACarray clusters cells by type and provides greater sensitivity than whole tissue arrays. FIG. 19 is comprised of Panels A, B, and C. Panel A of FIG. 19 shows hierarchical clustering of cell types and tissue samples. Panel B of FIG. 19 shows relative number of probesets detected by Bacarray. Panel C of FIG. 19 shows enrichment of genes undetected by whole tissue array.

FIG. 20 illustrates cell type diversity is driven by proteins on the cell surface, such as receptors and ion channels. FIG. 20 is comprised of Panels A, B, and C. Panel A of FIG. 20 shows average Shannon entropy of BACarray samples. Panel B of FIG. 20 shows examples of probeset with low and high information. Panel C of FIG. 20 shows the ten percent of the probesets with highest entropy and those with the lowest entropy classified with Gene Ontologies and then searched for functional categories that were over-represented.

FIG. 21 illustrates that a comparative analysis reveals unique translational profiles for each cell type. An iterative comparison was performed: one-by-one, each sample was compared to each other sample in the dataset, and for each population, probesets were sorted by their average ranking across these comparisons. FIG. 21 is comprised of Panels A and B. Panel A of FIG. 21 shows data combined and clustered by expression the top one hundred ranked probesets for each population in a heatmap. This heat map readily illustrates the extent to which distinct cell types are characterized by specific cohorts of genes. Panel B of FIG. 21 shows the top twenty five most specific probesets in each cell type include probesets for both well-known cell-specific markers and novel, previously uncharacterized genes.

FIG. 22 illustrates the transcriptional sketch of a spinal motor neuron.

FIG. 23 illustrates expression of eGFP-L10a in the Chat and Purkinje cell BACarray lines. (A) Immunohistochemistry to eGFP in adult sagittal sections from the Chat BACarray line DW167. (B) Indirect immunofluorescent characterization of Chat BACarray line DW167 brain stem facial motor nucleus: eGFP staining (left panel); Chat staining (middle panel) and merge (right panel, with 20 um scale bar). (C) Immunohistochemistry to eGFP in adult sagittal sections from the Pcp2 BACarray line DR166. (D) Indirect immunofluorescent characterization of Pcp2 BACarray line DR 166 Purkinje cell neurons: eGFP staining (left panel); Calbindin-D28K staining (middle panel) and merge (right panel, with 20 um scale bar).

FIG. 24 illustrates that BACarray profiles recapitulate known cell-specific markers and reveal new ones for four distinct cell types. Scatterplots of D1, D2, Chat, and Pcp2 BACarray data compared to a reference mRNA sample reveal hundreds of genes enriched in each cell type (A-D). Lines on either side of the diagonal mark 2-fold enrichment. Axes are labeled for expression in powers of 10. Venn diagrams of the top 1,000 enriched probesets (Tables 17-20) (with expression value cut-off >100) for each cell type reveal that each cell type has a unique pattern of enriched genes (E-H).

FIG. 25 illustrates expression of eGFP L10a in D1 and D2 BACarray lines. (a) Immunohistochemistry to eGFP in adult sagittal sections from the D2 BACarray line CP101. (b) Characterization of D2 BACarray line CP101 striatal MSN cells: direct eGFP fluorescence (left panel with high magnification image insert); enkephalin immunohistochemical staining (middle panel); merge (right panel, with 20 μm scale bar). (c) Immunohistochemistry to eGFP in adult sagittal sections from the D1 BACarray line CP73. (d) Characterization of D1 BACarray line CP73 striatal MSN cells: direct eGFP fluorescence (left panel); enkephalin immunohistochemical staining (middle panel); merge (right panel).

FIG. 26 illustrates the polysome profile from D2 BACarray mouse striatal extracts.

FIG. 27 illustrates an analysis of striatal MSN IP values relative to message abundance and length. Lengths of transcripts were based on all available mouse curated RefSeq RNA sequences (file transfer protocol address: ncbi.nih.gov/genomes/M_musculus/RNA). Where multiple transcript variants for a single gene were available, the longest one was chosen. RefSeq lengths were plotted against D1 (a) or D2 (b) BACarray IP normalized expression values. There was no correlation observed between transcript length and IP values. Striatal expression values for all Affymetrix Genechip probe sets were obtained by total RNA arrays from wild type striatal tissue (data not shown). These values were plotted against (c) D1 BACarray or (d) D2 BACarray IP normalized values. As expected, higher expression in total striatum (no IP, wild type mice) correlates with higher D1 or D2 BACarray IP values. The few genes that show modest expression in total striatum but have low IP values include known non neuronal genes.

FIG. 28 illustrates gene expression analysis of BACarray purified mRNA. Normalized expression values from Affymetrix Mouse Genome 430 2.0 arrays are plotted for D1 and D2 BACarray samples. Middle diagonal line represents equal expression, and lines to each side represent 1.5 fold enrichment in either cell population.

FIG. 29 illustrates expression analysis of medium spiny neuron (MSN) enriched genes. Expression analysis in sagittal sections of genes which were amongst the top 100 (a) or 1,000 1,100 (b) genes identified in the study as MSN enriched, with the rank order of each gene noted below the gene name. Non redundant gene ranking was calculated using the highest ranked probeset corresponding to each gene with redundant probesets eliminated. Left panel, in situ hybridization images taken from the Allen Brain Atlas (Allen Brain Atlas [Internet]. Seattle (Wash.): Allen Institute for Brain Science.© 2006. Available from: world wide web address: brain map.org.) (3); right panel, in situ hybridization images taken from the Brain Gene Expression Map (BGEM) database (world wide web address: stjudebgem.org/) (4). Allen Brain Atlas images all correspond to adult brain; BGEM images all correspond to adult brain except for the following, for which the oldest available data were postnatal day 7 (P7): Drd2, Ppp1r1b, Dlx6, Gdnf, Bcl11b, Foxg1, Limd2, Fem1b, Dynll1, Atbf1, Foxo3a, Dnalc4, Mtmr7, Dnmt3a.

FIG. 30 illustrates that functional Gpr6 receptors are found in BAC D2 striatopallidal neurons but not BAC D1 striatonigral medium spiny neurons. (a) Projection of an eGFP labeled medium spiny neuron from a BAC D2 mouse. The cell was patched with a pipette containing Alexa 594 (50 μM) for visualization and Fluo 4 (200 μM) for measuring changes in intracellular Ca2+ (right). Cells were voltage clamped at −70 mV. A puffer pipette containing sphingosine 1 phosphate (S1P, 10 μM) was positioned near a dendrite, 60 80 μm from the soma (left/cartoon). (b) High magnification images of a dendritic segment (control, left panel) show an increase in Ca2+ associated with S1P application (S1P puff, center panel) that reversed with washing (wash, right panel). The change in Ca2+ was determined by calculating the percent change in fluorescence of Fluo 4 relative to that of Alexa 594 (ΔG/R). (c) Time course showing the S1P induced increase in intracellular Ca2+ in the ROI from b (orange trace); similar recordings from BAC D1 medium spiny neurons (black trace) or thapsigargin loaded BAC D2 medium spiny neurons did not reveal any changes in dendritic Ca2+ levels with S1P application. (d) Box plot summarizing the S1P effects. Percent increase in fluorescence (ΔG/R) in BAC D2 medium spiny neurons (median=146%, range 44 to 294%, n=6); BAC D1 medium spiny neurons (median=17%, range 13 to 22%, n=4); and thapsigargin loaded BAC D2 medium spiny neurons (median=4%, range −9 to 10%, n=4).

FIG. 31 illustrates that cocaine treatment increases the frequency of small amplitude GABAergic mIPSCs in BAC D1 striatonigral neurons. (a) Representative spontaneous mIPSCs traces from BAC D1 striatonigral neurons (expressing soluble eGFP under the D1 promoter) taken from mice treated for 15 days with saline or (b) cocaine (20 mg/kg/day). (c) Bar graph summary of mean mIPSC frequency showing a increase in BAC D1 striatonigral neuron mIPSCs frequency following cocaine treatment (Mann Whitney Rank Sum Test, p<0.05, saline median=0.82 Hz, n=22; cocaine median=1.03 Hz, n=26). (d) Bar graph summary showing that the number of small amplitude mIPSCs (<75 pA) in equal length records (7 min) increased in BAC D1 striatonigral neurons following cocaine treatment (t test, p<0.05, saline=79.4±8.2, n=22; cocaine=104.2±6.3, n=26). (e) Representative variance mean current plots from saline treated and cocaine treated BAC D1 neurons suggesting that the cocaine induced small amplitude events arise from synapses that have fewer GABAA receptors (N) per synapse but receptors with an unchanged unitary receptor conductance (g) (saline N=33, g=31 pS; cocaine N=27, g=30 pS; see Fig. S6c d for means). (f) Representative spontaneous mIPSCs traces from BAC D2 striatopallidal neurons following saline treatment for 15 days and (g) following cocaine treatment for 15 days. (h) Bar graph summary of mean mIPSC frequency in saline and cocaine treated neurons, showing no effect of treatment condition (t test, p>0.05, saline=0.72±0.05 Hz, n=12; cocaine=0.78±0.07 Hz, n=16). (i) Bar graph summary showing that the number of small amplitude mIPSCs (<75 pA) in equal length records (7 min) was not altered by treatment condition in BAC D2 neurons (t test, p>0.05, saline=68.1±9.7, n=12; cocaine=71.7±7.5, n=16). (j) Representative variance mean current plots showing that cocaine treatment did not change in the number of receptors per synapse or the unitary receptor conductance in BAC D2 neurons (saline N=29, g=33pS; cocaine N=31, g=29pS;).

FIG. 32 illustrates that cocaine treatment decreases the number of GABAA channels per synapse in BAC D1 striatonigral neurons. Box plot summaries showing mean mIPSCs kinetics and non stationary noise analysis measures of individual striatonigral neurons taken from saline treated control and 15 day treated cocaine BAC D1 mice. (a) Neither mean 10 90% rise times (t test, p>0.05, Saline=0.64±0.008, n=22; Cocaine=0.65±0.006, n=26) nor (b) mean decay times (ttest, p>0.05, Saline=17.3±0.5, n=22; Cocaine=17.0±0.5, n=26) were different between groups. Non stationary noise analysis of mIPSCs demonstrated no change between groups in the (c) mean unitary conductance (g) of GABAA receptors of striatonigral neurons (t test, p>0.05, Saline g=31.1±1.6, n=22; Cocaine g=30.8±1.2, n=26), and a decrease in the (d) mean number of channels per synapse in striatonigral neurons taken from cocaine treated BAC D1 mice (t test, p<0.05, Saline N=33.1±1.4, n=22; Cocaine N=29.3±1.0, n=26).

FIG. 33 illustrates that cocaine treatment does not alter GABAergic mIPSCs kinetics or GABAA receptor number per synapse or unitary receptor conductance in BAC D2 striatopallidal neurons. Box plot summaries showing mean mIPSCs kinetics and non stationary noise analysis measures of individual striatopallidal neurons taken from saline treated control and 15 day treated cocaine BAC D2. (a) Neither mean 10 90% rise times (t test, p>0.05, Saline=0.65±0.007, n=12; Cocaine=0.64±0.010, n=16) nor (b) mean decay times (t test, p>0.05, Saline=18.0±1.0, n=12; Cocaine=18.0±0.5, n=16) were different between groups. Non stationary noise analysis of mIPSCs demonstrated no change between groups in the (c) mean unitary conductance (referred to as g in the y-axis) of GABAA receptors of striatopallidal neurons (t test, p>0.05, Saline g=30.7±1.5, n=12; Cocaine g=30.3±1.2, n=16) or in the (d) mean number of channels per synapse in striatopallidal neurons taken from cocaine treated BAC D2 mice (t test, p>0.05, Saline N=33.8±2.7, n=12; Cocaine N=33.0±1.6, n=16).

FIG. 34 illustrates BACarray molecular phenotyping of cerebellar cell types in ATM KO mice.

FIG. 35 illustrates the characterization of Pcp2 and Sept4 BACarray transgenic mouse lines. (A) Expression of the eGFP L10a fusion in Purkinje cells in the cerebellum of the Pcp2 BACarray line. Immunohistochemical stain of Pcp2 BACarray brain section using anti eGFP antiserum (top panel). Double immuno fluorescence analysis (bottom panels) of calbindin (Calb1) and eGFP L10a expression in Pcp2 BACarray mice showing co labeling of Purkinje cells (merge). (B) Expression of the eGFP L10a fusion in Bergmann glia in the cerebellum of the Sept4 BACarray line. Immunohistochemical stain of Sept4 BACarray brain section using anti eGFP antiserum (top panel). Double immuno fluorescence analysis of glutamine synthetase (Glns) and eGFP L10a expression in Sept4 BACarray mice showing co labeling of Bergmann glial cells (merge).

FIG. 36 illustrates a scatter Plot analysis of BACarray data for Septin4 and Pcp2 lines. Immunoprecipitated (IP) mRNA from Septin4 (A, C) and Pcp2 (B, D) BAC transgenic lines was directly compared in scatter plot analysis to mRNA samples from total cerebellum. The analysis clearly demonstrates that thousands of genes are enriched in the immunoprecipitated samples relative to total cerebellum. Scatter plots (C) and (D) show the same experiments with known positive control genes for Bergmann glial cells and Purkinje neurons. Bergmann glial positive controls are clearly enriched in the Septin4 IP samples (C) whereas Purkinje cell positive control genes are enriched in Pcp2 IP samples (D).

FIG. 37 illustrates cell type specific differential regulation of gene expression in Atm −/− cerebellum. (A-C) Atm −/− IP data were compared to Atm +/+ IP data in scatter plot analyses for the Septin4 (A), the Pcp2 (B) and the total cerebellum (C) experiments. Gene lists of both upregulated and downregulated genes were generated by filtering on expression values greater than 25, on fold change values greater than 1.5, and performing one way ANOVA, p=0.05. Scatter plots display the distribution of regulated genes in each experiment. Colors refer to the Venn Diagram analysis (D). Genes shared by Bergmann glia and Purkinje cells are colored yellow, those shared by Purkinje cells and total cerebellum are turquoise, and those shared by Bergmann glia and total cerebellum are colored magenta. Genes identified in all three conditions are colored white. (D) Venn Diagram analysis of all regulated genes. In total cerebellum, 40/69 genes are found differentially regulated exclusively in total cerebellum, in the Septin4 experiment, 94/116 genes are specifically regulated in the Septin4 data, and in the Pcp2 experiment, 62/89 regulated genes which are specific to the Pcp2 data have been identified.

FIG. 38 illustrates ventral tegmental area and substantia nigra area specific markers, as revealed by BACarrays.

FIG. 39 illustrates dopaminergic cell loss in the rodent substantia nigra with the use of 6-OHDA.

FIG. 40 illustrates a therapeutic opportunity for Parkinson's disease, by developing new strategies for stimulation of Sub-Thalamic Nucleus, by using antagonists of Gpr6.

DETAILED DESCRIPTION OF THE INVENTION

Translational Profiling and Molecular Phenotyping

The present disclosure provides for methods and compositions useful in translational profiling and molecular phenotyping of heterogeneous tissues and cell types. In one embodiment the methods can be used to define previously undefined cell types, identify molecular targets for diseases and disorders, and provide a way in which to identify co-regulated gene sets for particular biological and/or medically relevant functions.

Translational profiling is the profiling, identification, or isolation of translated mRNAs. In some embodiments such profiling is a measure of the nascent proteome. In other embodiments, this profiling allows for the identification of mRNAs being actively translated, or otherwise associated with the cellular translational machinery. Molecular phenotyping is the molecular and/or gene expression description of organs, tissues, and cell types.

The present disclosure provides for methods and compositions to practice translating ribosome affinity purification (TRAP) profiling methodology. In one embodiment these profiling methods are be utilized to further distinguish morphologically, anatomically, developmentally, or otherwise indistinguishable, cells into cellular subtypes, further defining cell populations and sub-populations. In some cases, these otherwise indistinguishable cells are intermixed. In other cases, these cells are spatially separated. In some cases, these cells are cells of the central or peripheral nervous system, for example neurons or glia, only distinguishable by their translational profiles and molecular phenotypes. In other cases these are cells outside the nervous system. In another embodiment these profiling methods are be utilized to identify translated mRNAs in single cell types with a sensitivity that is may not be achievable by techniques such as whole-tissue microarray. In yet other embodiments, these profiling methods are used to identify co-regulated gene sets for particular biological functions in cells that are spatially separated or intermixed, morphologically distinct or indistinct, in different developmental stages, associated with different tissues or regions of a tissue, or from different individuals with the same disease, disorder or state.

The methods provided herein allow for isolation of mRNAs associated with ribosomes or polysomes (clusters of ribosomes) from specific cell types, allowing for cell translational profiling and molecular phenotyping of the cell types and subtypes. In some cases, the cells are genetically targeted.

The methods described herein in allow for identifying translated mRNAs in any cell subtype of interest. The methodology involves expression of a tagged functional ribosomal protein, which enables tagging of polysomes for purification of mRNA, in specific cell populations. In some embodiments, the purification of polysomes is by affinity or immunoaffinity purification. In some embodiments, the cell subtypes are genetically targeted. In some embodiments the tagged ribosomes are expressed in transgenic animals

The translating ribosome affinity purification methodology (TRAP) for translational profiling and molecular phenotyping involves expression of a tagged ribosomal protein, which enables tagging of polysomes for affinity-based, sometimes immunoaffinity-based, purification of mRNA, in specific cell populations. In some cases this is achieved with the use of animals or transgenic animals, allowing translation profiling and molecular phenotyping from whole animals Methods provided herein allow for defining the distinguishing molecular characteristics of closely related or critical cell types. Methods also demonstrate that methods described herein can be employed to analyze physiological adaptations of specific cell types in vitro, in vivo, ex vivo, or in situ.

Molecular Tagging of Ribosomes

Embodiments of this invention provide methods for isolating cell type- or cell subtype-specific polysomal, mRNA. The methods include using molecularly tagged ribosomal proteins resulting in functional tagged ribosomes or ribosomal complexes, able to support translation, assemble into polysomal complexes. Ribosomes can be molecularly tagged and expressed in one or more cell types or cell subtypes of interest. Molecularly tagged ribosomal proteins for the purposes of this disclosure will interchangeably be referred to as a ‘fusion proteins’ or ‘tagged proteins’ or ‘molecularly tagged fusion proteins’ or ‘molecularly tagged ribosomal proteins’. In various embodiments these fusion proteins contain all or a portion of a ribosomal proteins. In certain embodiments ribosome tagging causes no disruption of native function or distribution of the tagged protein, thus resulting in a functional ribosome, functional ribosomal complex, or functional polysome. That is, although molecularly tagged, the portion that has the biological activity of the native ribosomal protein is retained and can function in an intact ribosome to carry out translation or binding of mRNA. In some embodiments, the molecularly tagged ribosomal is expressed in a organ, tissue, cell type, or cell subtype of interest, by introducing into cells, culture, slices, or into an entire organism, a nucleic acid encoding the molecularly tagged ribosomal protein under the control of regulatory elements or transcriptional units that direct expression in the cell type or cell subtype of choice. In some embodiments the ribosomal protein to be tagged can be from the same or different species as the cell that expresses the molecularly tagged protein.

In certain embodiments, the ribosome or ribosomal protein is molecularly tagged by engineering the ribosomal protein to fuse to or bind a small molecule, a protein, or peptide that is not bound by the unengineered ribosome or the ribosomal protein. In certain embodiments the nucleic acid encoding the ribosomal protein fused to the tag can be generated by routine genetic engineering methods in which a nucleotide sequence encoding the amino acid sequence for the tag sequence is engineered in frame with the nucleotide sequence encoding a ribosomal protein. This can be accomplished by any method known in the art, for example, via oligonucleotide-mediated site-directed mutagenesis or polymerase chain reaction (PCR) and other routine protocols of molecular biology (see, e.g., Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y., both of which are hereby incorporated by reference in their entireties).

In exemplary embodiments the ribosomal protein is L10a and the tag is eGFP.

1) Ribosomal Proteins

Nucleic acids encoding the tagged proteins can be produced using genetic engineering methods and cloning and expression vectors that are well known in the art. In some embodiments, a naturally occurring or synthetically produced nucleic acid or gene can be engineered to function as a ribosomal protein and support translation or bind (directly or indirectly) or associate with polysomal, translating ribosomal mRNA. Nucleic acids encoding the ribosomal protein to be molecularly tagged may be obtained using any method known in the art. Nucleic acids may be obtained, for example, by PCR using oligonucleotide primers based upon the published sequences. Other related ribosomal (for example from other species) may be obtained by low, medium or high stringency hybridization of appropriate nucleic acid libraries using the ribosomal in hand as a probe.

Exemplary ribosomal proteins for use in various embodiments of this invention are provided in

Table 1, but not limited to those listed. In an exemplary embodiment, the ribosomal protein is L10a. In some embodiments the tagged ribosomal protein is incorporated into a ribosomal complex and is associated with one or more mRNAS, but does not bind mRNA directly.

TABLE 1 Ribosomal Proteins A52 L11 L23a L35a LP2 (Large P2) S11 S24 Ke-3 L12 L24 L36 LP1 (Large P1) S12 S25 L3 L13 L26 L36a S2 S13 S26 L3L (L3-like) L13a L27 L37 S3 S14 S27 L4 L14 L27a L37a S3a S15 S27a L5 L15 L28 L38 S4 S15a S28 L6 L17 L29 L39 S5 S16 S29 L7 L18 L30 L41 S6 S17 S30 L7a L18a L31 L44 S7 S18 S23 L8 L19 L32 LAMR1 S8 S19 RPLP1 L9 L21 L32-3a (3a) LLRep3 S9 S20 L10 L22 L34 LP0 (Large P0) S10 S21 L10a L23 L35 Region containing hypothetical protein FLJ23544

In certain embodiments, the ribosomal protein is mouse ribosomal protein L10a having the amino acid sequence set forth in GenBank NM_011287.1 (SEQ ID NO: 30).

In certain embodiments, a nucleic acid encoding a molecularly tagged ribosomal protein is intended for a particular expression system, in which the codon frequencies reflect the tRNA frequencies of the host cell or organism in which the protein is expressed. Codon optimization can allow for maximum protein expression by increasing the translational efficiency of a gene of interest. In other embodiments the nucleic acid encoding a molecularly tagged ribosomal protein may be a synthetic nucleic acid in which the codons have been optimized for increased expression in the host cell in which it is produced.

2) Molecular Tags

Molecular tags can be any protein (or fragment, portion, analog or derivative thereof) that is not present or accessible in the cell of interest (or the cell fraction from which the tagged ribosomes are to be isolated, or other cells that will be contacted with the reagent that binds the tag) for which there exists a reagent (such as an antibody) or method (such as optical, fluorescence or magnetic sorting) that recognizes the tag and that is accessible to solution (and thereby, the tag).

Molecular tagging with tags that are traditionally utilized as reporter genes is well known in the art (Current Protocols in Molecular biology, Section 9.6.1, “Uses of Fusion Genes in mammalian Transfection” (2004)). Molecular tagging with epitopes are also well known in the art (“epitope tagging” reviewed in Fritze C E, Anderson T R. Epitope tagging: general method for tracking recombinant proteins. Methods Enzymol. 2000; 327:3-16; Jarvik J W, Telmer C A. Epitope tagging. Annu Rev Genet. 1998; 32:601-18).

Tags can include those for which methods/reagents/devices exist that allow facile identification and isolation of the tagged protein, but do not, minimally, or negligibly inhibit or interfere with function of the tagged protein. The tag may be an intact naturally occurring or synthetically produced protein, fragment, analog or derivative thereof of any length that permits binding to the corresponding binding reagent/method. In certain embodiments, the tag is about 8, 10, 12, 15, 18 or 20 amino acids, is less than 15, 20, 25, 30, 40 or 50 amino acids, but may be 100, 150, 200, 250, 300, 400, 500, 1000 or more amino acids in length. The tag may be bound specifically by a reagent that does not otherwise bind any component of: (1) the cell of interest; or (2) a polysomal preparation of interest; or (3) whatever cellular fraction of interest is being contacted by the reagent that binds the tag.

In certain embodiments, the nucleotide sequence encoding a tag is preferably inserted in frame such that the tag is placed at the N- or C-terminus of the ribosomal protein, since these portions of proteins are often accessible to detection, affinity, immunological, or purification reagents. Alternatively, the tag can be inserted into any portion of the ribosomal protein such that when the fusion protein is incorporated into an intact ribosome, ribosomal function is not compromised and the tag is accessible to the reagent/method to be used in the detection, isolation or and/or purification. That is, tagging can still allow for a functional ribosome. In other embodiments, a ribosomal protein may be molecularly tagged with a plurality of tags.

In some embodiments, the tag is Green Fluorescent Protein (GFP). GFP can be utilized as an optical or immunoaffinity tag for a variety of applications, all well known in the art. GFP is a protein, composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish that fluoresces green when exposed to blue light (Prendergast F, Maim K (1978). “Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea”. Biochemistry 17 (17):3448-53; Tsien R (1998). “The green fluorescent protein”. Annu Rev Biochem 67: 509-44). The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression (Phillips G (2001). “Green fluorescent protein—a bright idea for the study of bacterial protein localization”. FEMS Microbiol Lett 204 (1): 9-18.). In modified forms it has been used to make biosensors, and many animals have been created that express GFP. The GFP gene can be introduced into organisms and maintained in their genome through breeding, or local injection with a viral vector can be used to introduce the gene. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been created using GFP as a marker.

In other embodiments, the tag is a GFP mutant, variant, analog, fragment, or derivative thereof. Different mutants of GFP have been engineered (Shaner N, Steinbach P, Tsien R (2005). “A guide to choosing fluorescent proteins”. Nat Methods 2 (12): 905-9). One improvement was a single point mutation (S65T) reported in 1995 in Nature by Roger Tsien (Heim R, Cubitt A, Tsien R (1995). “Improved green fluorescence”. Nature 373 (6516): 663-4.)). The addition of the 37° C. folding efficiency (F64L) point mutant to the scaffold yielded enhanced GFP (eGFP). eGFP has an extinction coefficient (denoted ε), also known as its optical cross section of 9.13×10-21 m²/molecule, also quoted as 55,000 L/(mol·cm) (Shelley R. McRae, Christopher L. Brown and Gillian R Bushell (May 2005)). “Rapid purification of eGFP, EYFP, and ECFP with high yield and purity”. Protein Expression and Purification 41 (1): 121-127.). Superfolder GFP, a series of mutations that allow GFP to rapidly fold and mature even when fused to poorly folding peptides, was reported in 2006 (Pédelacq J, Cabantous S, Tran T, Terwilliger T, Waldo G (2006). “Engineering and characterization of a superfolder green fluorescent protein”. Nat Biotechnol 24 (1): 79-88). In an exemplary embodiment the tag is enhanced GFP (eGFP).

Other mutations have been made, including color mutants; in particular blue fluorescent protein (EBFP, EBFP2, Azurite, mKalamal), cyan fluorescent protein (ECFP, Cerulean, CyPet) and yellow fluorescent protein derivatives (YFP, Citrin, Venus, YPet). BFP derivatives (except mKalamal) contain the Y66H substitution. The mutation in cyan derivatives is the Y66W substitution. The red-shifted wavelength of the YFP derivatives is accomplished by the T203Y mutation (Tsien R (1998). “The green fluorescent protein”. Annu Rev Biochem 67: 509-44).

Molecular tags may include both fluorescent, magnetic, epitope, radioactive or otherwise detectable tags, by way of example, and not by limitation, those listed in Table 2.

TABLE 2 Molecular Tags GFP, eGFP, mGFP, emerald GFP, derivatives and variants (Green Fluorescent protein) BFP, eBFP, eBFP2, Azurite, mKalamal, sapphire GFP, derivatives and variants (Blue fluorescent proteins) ECFP, Cerulean, CyPet, DFP, TFP, mTFP, derivatives and variants (cyan and teal fluorescent proteins) YFP, Citrine, Venus, YPet, Topaz GFP, derivatives and variants (yellow fluorescent proteins) RFP, mCherry, td-tomato, derivatives and variants (red fluorescent protein) Bgal, derivatives and variants (Bgalactosdiase) Luc, derivatives and variants (Luciferase) CAT, derivatives and variants (Chloramphenicol acetyltransferase) hGH, derivatives and variants (human growth hormone) Protein A fragments; Myc epitopes Btag and polyhistidine tracts Influenza virus hemagglutinin protein, fragments, derivatives, variants C-myc gene, fragments, derivatives, variants Bluetongue virus VP7 protein, fragments, derivatives, variants FLAG peptide, fragments, derivatives, variants Strep-tag peptide, fragments, derivatives, variants pHlourins, fragment, derivatives, variants

References for Table 2 are provided here, and incorporated by reference herein: Evan et al., Mol. Cell Biol. 5(12):3610-3616; Wang et al., 1996, Gene 169(1):53-58; Bornhorst et al., 2000, Purification of proteins using polyhistidine affinity tags, Methods Enzymol 326:245-54; Wilson I A, Niman H L, Houghten R A, Cherenson A R, Connolly M L, Lemer R A. The structure of an antigenic determinant in a protein. Cell. 1984 July; 37(3):767-78; Evan G I, Lewis G K, Ramsay G, Bishop J M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985 December; 5(12): 3610-6; Wang L F, Yu M, White J R, Eaton B T. BTag: a novel six-residue epitope tag for surveillance and purification of recombinant proteins. Gene. 1996 Feb. 22; 169(1):53-8; Hopp et al., U.S. Pat. No. 4,703,004, entitled “Synthesis of protein with an identification peptide” issued Oct. 27, 1987; Brizzard B L, Chubet R G, Vizard D L. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 1994 April; 16(4):730-5; Knappik A, Pluckthun A. An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments. Biotechniques. 1994 October; 17(4):754-761; Skerra et al., U.S. Pat. No. 5,506,121, entitled Fusion peptides with binding activity for streptavidin, issued Apr. 9, 1996; Skerra A, Schmidt T G. Applications of a peptide ligand for streptavidin: the Strep-tag. Biomol Eng. 1999 Dec. 31; 16(1-4):79-86; Skerra A, Schnudt T G. Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins. Methods Enzymol. 2000; 326:271-304; Shaner N, Steinbach P, Tsien R (2005). “A guide to choosing fluorescent proteins”. Nat Methods 2 (12): 905-9. doi:10.1038/nmeth819. PMID 16299475; Shaner N, Steinbach P, Tsien R (2005). “A guide to choosing fluorescent proteins”. Nat Methods 2 (12): 905-9. PMID 16299475; Heim R, Cubitt A, Tsien R (1995). “Improved green fluorescence”. Nature 373 (6516): 663-4. PMID 7854443; Shelley R. McRae, Christopher L. Brown and Gillian R Bushell (May 2005). “Rapid purification of eGFP, EYFP, and ECFP with high yield and purity”. Protein Expression and Purification 41 (1): 121-127; Pédelacq J, Cabantous S, Tran T, Terwilliger T, Waldo G (2006). “Engineering and characterization of a superfolder green fluorescent protein”. Nat Biotechnol 24 (1): 79-88. PMID 16369541; Miesenbock G, De Angelis D, Rothman J (1998). “Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins”. Nature 394 (6689): 192-5. PMID 9671304.

Isolation of Ribosomes, Polysomes, mRNA

1) Isolation of Ribosomes

Various methods exist to isolate ribosomes/tagged ribosomes, particularly polysomes (ribosomal clusters bound to mRNA)/tagged polysomes, from cells, cultured cells and tissues (see, e.g., Bommer et al., 1997, Isolation and characterization of eukaryotic polysomes, in Subcellular Fractionation, Graham and Rickwood (eds.), IRL Press, Oxford, pp. 280-285; incorporated herein by reference in its entirety). Polysomes are interchangeably referred to as polyribosomes, ribosomal complexes or ribosomal clusters. In preferred embodiments, the isolated polysomes (ribosomal-mRNA complexes) contain functional ribosomes, capable of supporting translation, association with mRNA, and/or association with translation factors.

In certain embodiments, the isolation method employed has one or more of the following aspects:

-   -   a. Maintenance of ribosomal subunits on mRNA during isolation:         translation arresting compounds, such as emetine or         cycloheximide can be added to arrest translation, whereby         reducing or preventing dissociation of mRNA from the ribosome.         In preferred embodiments isolation is achieved without         crosslinking and crosslinking reagents;     -   b. Inhibition of endogenous RNAase activity: RNAase inhibitors         can be added to buffers to maintain the integrity of the mRNA;     -   c. Isolation of Polysomes: After tissue or cell homogenization,         total polysomes are isolated by preparing a post-mitochondrial         supernatant in the presence of at least a high concentration         salt buffer, for example about 100-150 mM KCl; and     -   d. Solubilization of rough ER-bound Polysomes under         non-denaturing conditions: Detergent can also be added to         release membrane-associated polysomes from endoplasmic reticulum         membranes; total polysomes are usually collected by         centrifugation through, for example, a sucrose cushion.

In other embodiments, variations of the above-described general method are used to isolate membrane-associated polysomes from a total pool of polysomes. This allows for further enrichment of mRNA encoding secreted or transmembrane proteins. Various methods may be used to isolate membrane-associated polysomes from cultured cells and tissue, e.g., methods that employ differential centrifugation (Hall C, Lim L. Developmental changes in the composition of polyadenylated RNA isolated from free and membrane-bound polyribosomes of the rat forebrain, analyzed by translation in vitro. Biochem J. 1981 Apr. 15; 196(1):327-36), rate-zonal centrifugation (Rademacher and Steele, 1986, Isolation of undegraded free and membrane-bound polysomal mRNA from rat brain, J. Neurochem. 47(3):953-957), isopycnic centrifugation (Mechler, 1987, Isolation of messenger RNA from membrane-bound polysomes, Methods Enzymol. 152: 241-248), and differential extraction (Bommer et al., 1997, Isolation and characterization of eukaryotic polysomes, in Subcellular Fractionation, Graham and Rickwood (eds.), IRL Press, Oxford, pp. 280-285; incorporated herein by reference in its entirety) to isolate the membrane-associated polysomes (Heintz US publication 20050009028 incorporated in its entirety).

In other embodiments, affinity methods are used to isolate or purify tagged proteins using methods well known in the art including but not limited to including chromatography, solid phase chromatography precipitation, matrices, co-immunoprecipitation, etc.

In specific embodiments, molecularly tagged functional ribosomes are provided, bound to mRNA, that can bind a reagent for the molecular tag. In other embodiments, the molecularly tagged ribosomes are bound to a specific reagent or affinity reagent that is bound, covalently or non-covalently, to a solid surface, such as a bead, a resin, or a chromatography resin, e.g., agarose, sepharose, and the like. In other embodiments, other methods are used with or in place of affinity purification. In other embodiments, specific polysomes can be isolated utilizing optical sorting, fluorescence-based sorting or magnetic-based sorting methods and devices.

In certain embodiments, polysomes are not isolated from the post-mitochondrial supernatant or even from a cell or tissue lysate before being subject to affinity purification.

FIG. 2a presents a schematic to illustrate affinity purification of eGFP-tagged polysomes using anti-GFP antibody-coated beads.

2) Isolation of mRNA from Ribosomes

Once the tagged ribosome has been isolated, the associated mRNA complexed with the protein may be isolated using chemical, mechanical or other methods well known in the art. For example, elution of mRNA is accomplished by addition of EDTA to buffers, which disrupts polysomes and allows isolation of bound mRNA for analysis (Schutz, et al. (1977), Nucl. Acids Res. 4:71-84; Kraus and Rosenberg (1982), Proc. Natl. Acad. Sci. USA 79:4015-4019). In addition, isolated polysomes (attached or detached from isolation matrix) can be directly inputted into RNA isolation procedures using reagents such as Tri-reagent (Sigma) or Triazol (Sigma). In particular embodiments, poly A.sup.+ mRNA is preferentially isolated by virtue of its hybridization of oligodT cellulose. Methods of mRNA isolation are described, for example, in Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y., both of which are hereby incorporated by reference in their entireties.

Nucleic Acid Sequences for Cloning, Expression and Regulation of Tagged Ribosomes

1) General

Methods that are well known to those skilled in the art can be used to construct vectors containing tagged ribosomal protein coding sequences operatively associated with the appropriate transcriptional and translational control signals required for expression. These methods include, for example, in vitro recombinant DNA techniques and in vivo genetic recombination. See, for example, the techniques described in Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y.

2) Regulatory Sequences

Certain embodiments of the invention provide vectors, cell lines, and lines of organisms that contain nucleic acid constructs that comprise the coding sequence for a tagged ribosomal protein under the control of regulatory sequences. According to the methods described herein, the tagged ribosomal proteins are selectively expressed in a particular chosen cell type, cell subtype, molecular pathway, or circuit using regulatory elements naturally occurring or engineered to recapitulate a desired expression pattern. Such expression is achieved by driving the expression of the tagged ribosomal protein using regulatory sequences or a transcriptional unit from a characterizing or endogenously regulated gene expressed in the chosen cell type, subtype, pathway, circuit and/or point in development. In certain embodiments a particular cell type is one that resides within a mixed population of cells, comprising a discernable group of cells sharing a common characteristic. This group may be morphologically, anatomically, genetically, or functionally discernible. In other embodiments, this group may be morphologically, genetically, and developmentally indistinguishable, but be genetically distinguishable. Because of its selective expression, the population of cells may be characterized or recognized based on its positive expression of an endogenously regulated characterizing gene. Some or all of the regulatory sequences/elements may be incorporated into nucleic acids (including transgenes) to regulate the expression of tagged ribosomal protein coding sequences. Examples of regulatory sequences or elements include but are not limited to enhancer sequences, insulator sequences, silencer sequences, or a combination of said sequences. In certain embodiments, a gene that is not constitutively expressed, (i.e., exhibits some spatial or temporal restriction in its expression pattern) is used as a source of a regulatory sequence. In other embodiments, a gene that is constitutively expressed is used as a source of a regulatory sequence. In other embodiments a regulatory sequence is engineered or modified to achieve desired expression characteristics and patterns.

Regulatory regions can be whole or parts or the regulatory sequences from the loci of the genes of interest. For example, to drive specific expression of a tagged ribosomal protein (for example eGFP tagged L10a) in medium spiny neurons, regulatory sequences from a medium spiny neuron-specific gene locus, for example a Drd1a or Drd2 locus, can be utilized. In another example, to drive specific expression of a tagged ribosomal protein (for example eGFP tagged L10a) in motor neurons, regulatory sequences from a motor-specific gene locus, for example choline acetyl transferase (Chat) locus, can be utilized. Similarly, to drive specific expression of a tagged ribosomal protein (for example eGFP tagged L10a) in cerebellar cells, regulatory sequences from a cerebellar cell-specific gene loci. For example loci from Pcp2, Neurod1, Grm2, Grp, Septin4, or Aldh1l1 can be utilized, to drive specific expression in Purkinje cells, granule cells, golgi neurons, unipolar brush cells, Bergmann glial cells, and astrocytes, respectively. An entire locus, an entire non-coding region of a locus, a portion of a locus, or a modified locus can be utilized as the regulatory sequences to drive expression of tagged ribosomal proteins in a genetically targeted, cell subtype specific manner.

The tagged ribosomal gene coding sequences may placed under the transcriptional control of some or all of the regulatory sequences from a particular locus such that the tagged ribosomal gene is expressed in substantially the same expression pattern as the endogenously regulated gene found in that locus in the transformed organism, or at least in an anatomical region or tissue of the organism (by way of example, in the brain, spinal cord, heart, skin, bones, head, limbs, blood, muscle, peripheral nervous system, etc. of an animal) containing the population of cells to be marked by expression of the tagged ribosomal protein gene coding sequences. By “substantially the same expression pattern” is meant that the tagged ribosomal protein gene coding sequences are expressed in at least 50%, 60%, 70% 80%, 85%, 90%, 95%, and about 100% of the cells shown to express the endogenous characterizing gene by in situ hybridization, PCR, other gene expression detection methods or functional methods familiar to those with skill in the art.

The regulatory sequence is, e.g., a promoter or enhancer, or any other cis or trans activating element or transcriptional unit of a characterizing gene. In some embodiments this characterizing gene is endogenous to a host cell or host organism (or is an ortholog of an endogenous gene) and is expressed in a particular select population of cells of the organism. The regulatory sequence can be derived from a human, rat, or mouse or any mammalian gene associated with specific neuronal, cellular, and metabolic pathways. In certain embodiments, a non mammalian regulatory sequence can be utilized. Cis and trans, 5′ and 3′ regulatory sequences and transcriptional units from several known pathways are amenable for cell-type specific, cell subtype-specific, developmental stage-specific, temporal-specific, tissue-specific, pathway-specific, or circuit-specific expression of transgenes. Examples of known pathways include but are not limited to the adrenergic or noradrenergic neurotransmitter pathway, the dopaminergic neurotransmitter pathway, the GABAergic neurotransmitter pathway, the glutaminergic neurotransmitter pathway, the glycinergic neurotransmitter pathway, the histaminergic neurotransmitter pathway, a neuropeptidergic neurotransmitter pathway, a serotonergic neurotransmitter pathway, a nucleotide receptor-specific pathway, ion channels, transcriptions factors, markers of undifferentiated or not fully differentiated cells, the sonic hedgehog signaling pathway, calcium binding, or a neurotrophic factor receptor (tables in US2005/0009028, herein incorporated by reference).

In certain embodiments regulatory sequences that may be used to control expression can be derived from, include but are not limited to, the following animal transcriptional control regions that exhibit tissue specificity and that have been utilized in cell culture and transgenic animals Examples of transcriptional control regions are: elastase I gene control region, enolase promoter, insulin gene control region, immunoglobulin gene control region, mouse mammary tumor virus control region, albumin gene control region, alpha-fetoprotein gene control region, alpha 1-antitrypsin gene control region, beta.-globin gene control region, myelin basic protein gene control region, myosin light chain-2 gene control region, and gonadotropic releasing hormone gene control region. In other embodiments, the gene sequence from which the regulatory sequence derives can be protein kinase C, gamma, TH-elastin, Pax7, Eph receptors, islet-1 and Sonic hedgehog.

Nucleic acids to be used for regulating expression may include all or a portion of the upstream and/or downstream and 5′ and/or 3′ regulatory sequences from the naturally occurring or modified locus of a selected gene. The regulatory sequences preferably direct expression of the tagged ribosomal protein sequences in substantially the same pattern as the endogenous characterizing gene within transgenic organism, or tissue derived therefrom. This would provide of for any and all cis- and trans-acting regulatory sequences and transcriptional units that would direct expression of the tagged ribosomal protein in an endogenous regulatory fashion. A regulatory sequence can be 1 kb, 5 kb, 10 kb, 50 kb, 100 kb, 200 kb, 250 kb, 500 kb or even 1 Mb in length.

In certain embodiments, the nucleic acids encoding the molecularly tagged ribosomal proteins may be selectively expressed in random but distinct subsets of cells, as described in Feng et al. (2000, Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP, Neuron 28(1):41-51, which is hereby incorporated by reference in its entirety). Using such methods, independently generated transgenic lines may express the nucleic acids encoding the molecularly tagged ribosomal proteins in a unique pattern, perhaps pathway-specific, or circuit-specific, and although spatially separated, would all incorporate identical regulatory elements.

3) Placement and Construction of Regulatory Sequences and Transgenes

In certain embodiments the nucleotide sequences encoding the tagged ribosomal protein product may replace all or a portion of the characterizing gene coding sequences in a genomic clone/locus of the characterizing gene, leaving only all or part of the characterizing gene regulatory non-coding sequences.

In other embodiments, the tagged ribosomal gene coding sequences are inserted into or replace transcribed coding or non-coding sequences of the genomic characterizing gene sequences, for example, into or replacing a region of an exon or of the 3′ UTR of the characterizing gene genomic sequence.

In one embodiment, the tagged ribosomal protein gene coding sequence is inserted into or replaces a portion of the 3′ untranslated region (UTR) of the characterizing gene genomic sequence. In another embodiment, the coding sequence of the characterizing gene is mutated or disrupted to abolish characterizing gene expression from the nucleic acid construct without affecting the expression of the tagged fusion protein gene. In certain embodiments, the tagged ribosomal fusion protein gene coding sequence has its own internal ribosome entry site (IRES).

In some embodiments, the tagged ribosomal protein gene coding sequences are inserted using 5′ direct fusion wherein the tagged ribosomal protein gene coding sequences are inserted in-frame adjacent to the initial ATG sequence (or adjacent the nucleotide sequence encoding the first two, three, four, five, six, seven or eight amino acids of the characterizing gene protein product) of the characterizing gene, so that translation of the inserted sequence produces a fusion protein of the first methionine (or first few amino acids) derived from the characterizing gene sequence fused to the tagged ribosomal fusion protein gene protein.

In other embodiments, a tagged ribosomal fusion protein gene is inserted into a separate cistron in the 5′ region of the characterizing gene genomic sequence and has an independent IRES sequence. In certain embodiments, an IRES is operably linked to the tagged ribosomal fusion protein gene coding sequence to direct translation of the tagged fusion protein gene. The IRES permits the creation of polycistronic mRNAs from which several proteins can be synthesized under the control of an endogenous transcriptional regulatory sequence. Such a construct is advantageous because it allows marker proteins to be produced in the same cells that express the endogenous gene (Heintz, 2000, Hum. Mol. Genet. 9(6): 937-43; Heintz et al., WO 98/59060; Heintz et al., WO 01/05962; which are incorporated herein by reference in their entireties).

In certain embodiments, exogenous translational control signals, including, for example, the ATG initiation codon, can be provided by the characterizing gene or some other heterologous gene. The initiation codon is usually in phase with the reading frame of the desired coding sequence of the tagged ribosomal fusion protein gene to ensure translation of the entire insert. These exogenous translational control signals and initiation codons can be of a variety of origins, both natural and synthetic. The efficiency of expression may be enhanced by the inclusion of appropriate transcription enhancer elements, transcription terminators, etc. (see Bittner et al., 1987, Methods in Enzymol. 153: 516-44).

The construct can also comprise one or more selectable markers that enable identification and/or selection of recombinant vectors. The selectable marker may be the tagged ribosomal fusion protein gene product itself or an additional selectable marker not necessarily tied to the expression of the characterizing gene.

In certain embodiments where a tagged ribosomal fusion protein gene is expressed conditionally, the tagged ribosomal fusion protein gene coding sequence is embedded in the genomic sequence of the characterizing gene and is inactive unless acted on by a transactivator or recombinase, whereby expression of the tagged ribosomal fusion protein gene can then be driven by the characterizing gene regulatory sequences. In such a system, expression of the transactivator gene, as regulated by the characterizing gene regulatory sequences, activates the expression of the tagged fusion protein gene.

In a specific embodiment, a nucleic acid of the invention is expressed conditionally, using any type of inducible or repressible system available for conditional expression of genes known in the art, e.g., a system inducible or repressible by tetracycline (“tet system”); interferon; estrogen, ecdysone, or other steroid inducible system; Lac operator, progesterone antagonist RU486, or rapamycin (FK506). For example, a conditionally expressible nucleic acid of the invention can be created in which the coding region for the tagged ribosomal fusion protein gene (and, optionally also the characterizing gene) is operably linked to a genetic switch, such that expression of the tagged ribosomal fusion protein gene can be further regulated. One example of this type of switch is a tetracycline-based switch.

In several embodiments, the nucleic acids of the invention comprise all or a significant portion of the genomic sequence of the characterizing gene locus, at least all or a portion of the 5′ and/or 3′ regulatory sequences of the characterizing endogenously regulated gene, at least sufficient sequence 5′ and/or 3′ of the characterizing gene coding sequence to direct expression of the tagged ribosomal fusion protein gene coding sequences in the same expression pattern (temporal and/or spatial) as the endogenous counterpart of the characterizing gene. In certain embodiments, the nucleic acid of the invention comprises at least one exon, at least two exons, at least three exons, all but one exon, or all but two exons, of the characterizing endogenously regulated gene.

4) Expression Using a Binary System

Since the level of expression of the tagged ribosomal protein within a cell may needed in the efficiency of the isolation procedure, in certain embodiments of the invention, a binary system can be used, in which the endogenous regulatory sequence such as a promoter drives expression of a protein that then activates a second expression construct. This second expression construct uses a strong regulatory sequence such as a promoter to drive expression of the tagged ribosomal fusion protein at higher levels than is possible using the endogenous regulatory region itself.

In certain embodiments, a particular population-specific gene drives expression of a molecular switch (e.g., a recombinase, a transactivator) in a population-specific manner. This switch then activates high-level expression though a second regulatory element regulating expression of the tagged ribosomal protein.

For example, the molecularly tagged ribosomal protein coding sequence may be expressed conditionally, through the activity of a molecular switch gene which is an activator or suppressor of gene expression. In this case, the second gene encodes a transactivator, e.g., tetR, a recombinase, or FLP, whose expression is regulated by the characterizing gene regulatory sequences. The gene encoding the molecularly tagged ribosomal protein is linked to a conditional element, e.g., the tet promoter, or is flanked by recombinase sites, e.g., FRT sites, and may be located any where within the genome. In such a system, expression of the molecular switch gene, as regulated by the characterizing gene regulatory sequences, activates the expression of the molecularly tagged ribosomal protein.

5) Transcriptional Regulation Systems

In certain embodiments, the tagged ribosomal fusion protein gene can be expressed conditionally by operably linking at least the coding region for the tagged ribosomal fusion protein gene to all or a portion of the regulatory sequences from the characterizing gene's genomic locus, and then operably linking the tagged ribosomal fusion protein gene coding sequences and characterizing gene sequences to an inducible or repressible transcriptional regulation system. Transactivators in these inducible or repressible transcriptional regulation systems are designed to interact specifically with sequences engineered into the vector. Such systems include those regulated by tetracycline (“tet systems”), interferon, estrogen, ecdysone, Lac operator, progesterone antagonist RU486, and rapamycin (FK506) with tet systems being particularly preferred (see, e.g., Gingrich and Roder, 1998, Annu. Rev. Neurosci. 21: 377-405; incorporated herein by reference in its entirety). These drugs or hormones (or their analogs) act on modular transactivators composed of natural or mutant ligand binding domains and intrinsic or extrinsic DNA binding and transcriptional activation domains. In certain embodiments, expression of the detectable or selectable marker can be regulated by varying the concentration of the drug or hormone in medium in vitro or in the diet of the transformed organism in vivo.

The inducible or repressible genetic system can restrict the expression of the detectable or selectable marker either temporally, spatially, or both temporally and spatially.

In other embodiments, expression of the tagged ribosomal fusion protein gene is regulated by using a recombinase system that is used to turn on or off tagged ribosomal fusion protein gene expression by recombination in the appropriate region of the genome in which the marker gene is inserted. Such a recombinase system, in which a gene that encodes a recombinase can be used to turn on or off expression of the tagged ribosomal fusion protein gene (for review of temporal genetic switches and “tissue scissors” using recombinases, see Hennighausen and Furth, 1999, Nature Biotechnol. 17: 1062-63). Exclusive recombination in a selected cell type may be mediated by use of a site-specific recombinase such as Cre, FLP-wild type (wt), FLP-L or FLPe. Recombination may be effected by any art-known method, e.g., the method of Doetschman et al. (1987, Nature 330: 576-78; incorporated herein by reference in its entirety); the method of Thomas et al., (1986, Cell 44: 419-28; incorporated herein by reference in its entirety); the Cre-loxP recombination system (Stemberg and Hamilton, 1981, J. Mol. Biol. 150: 467-86; Lakso et al., 1992, Proc. Natl. Acad. Sci. USA 89: 6232-36; which are incorporated herein by reference in their entireties); the FLP recombinase system of Saccharomyces cerevisiae (O'Gorman et al., 1991, Science 251: 1351-55); the Cre-loxP-tetracycline control switch (Gossen and Bujard, 1992, Proc. Natl. Acad. Sci. USA 89: 5547-51); and ligand-regulated recombinase system (Kellendonk et al., 1999, J. Mol. Biol. 285: 175-82; incorporated herein by reference in its entirety). Preferably, the recombinase is highly active, e.g., the Cre-loxP or the FLPe system, and has enhanced thermostability (Rodriguez et al, 2000, Nature Genetics 25: 139-40; incorporated herein by reference in its entirety).

In certain embodiments, a recombinase system can be linked to a second inducible or repressible transcriptional regulation system. For example, a cell-specific Cre-loxP mediated recombination system (Gossen and Bujard, 1992, Proc. Natl. Acad. Sci. USA 89: 5547-51) can be linked to a cell-specific tetracycline-dependent time switch detailed above (Ewald et al., 1996, Science 273: 1384-1386; Furth et al. Proc. Natl. Acad. Sci. U.S.A. 91: 9302-06 (1994); St-Onge et al, 1996, Nucleic Acids Research 24(19): 3875-77; which are incorporated herein by reference in their entireties).

In one embodiment, an altered cre gene with enhanced expression in mammalian cells is used (Gorski and Jones, 1999, Nucleic Acids Research 27(9): 2059-61; incorporated herein by reference in its entirety).

In a specific embodiment, the ligand-regulated recombinase system of Kellendonk et al. (1999, J. Mol. Biol. 285: 175-82; incorporated herein by reference in its entirety) can be used. In this system, the ligand-binding domain (LBD) of a receptor, e.g., the progesterone or estrogen receptor, is fused to the Cre recombinase to increase specificity of the recombinase.

6) Sources of Nucleic Acids

Nucleic acids comprising the characterizing gene sequences and tagged ribosomal fusion protein gene coding sequences can be obtained from any available source. In most cases, all or a portion of the characterizing gene sequences and/or the tagged ribosomal fusion protein gene coding sequences are known, for example, in publicly available databases such as GenBank, UniGene and the Mouse Genome Informatic (MGI) Database to name just a few, or in private subscription databases. With a portion of the sequence in hand, hybridization probes can be designed using highly routine methods in the art (for example filter hybridization or PCR amplification) to identify clones containing the appropriate sequences for example in a library or other source of nucleic acid. If the sequence of the gene of interest from one species is known and the counterpart gene from another species is desired, it is routine in the art to design probes based upon the known sequence. The probes hybridize to nucleic acids from the species from which the sequence is desired, for example, hybridization to nucleic acids from genomic or DNA libraries from the species of interest. By way of example and not limitation, genomic clones can be identified by probing a genomic DNA library under appropriate hybridization conditions, e.g., high stringency conditions, low stringency conditions or moderate stringency conditions, depending on the relatedness of the probe to the genomic DNA being probed.

7) Vectors

Briefly, the characterizing gene genomic sequences are preferably in a vector that can accommodate lengths of sequence (for example, 10 kb's of sequence), such as cosmids, yeast artificial chromosomes (YACs) and bacterial artificial chromosomes (BACs), and encompass at least 50, 70, 80, 100, 120, 150, 200, 250, 300, 400, 500 or 1000 kb of sequence that comprises all or a portion of the characterizing gene sequence. The larger the vector insert, the more likely it is to identify a vector that contains the characterizing gene cis and trans sequences of interest. In some embodiments the vector containing the entire genomic sequence (i.e. genomic locus) for the characterizing endogenously regulated gene is present. In other embodiments the entire genomic sequence cannot be accommodated by a single vector or such a clone is not available. In these instances (or when it is not known whether the clone contains the entire genomic sequence), the vector contains at least the characterizing gene sequence with the start, i.e., the most 5′ end, of the coding sequence in the approximate middle of the vector insert containing the genomic sequences and/or has at least 20 kb, 30 kb, 40 kb, 50 kb, 60 kb, 80 kb, 100 kb or 200 kb of genomic sequence on either side of the start of the characterizing gene coding sequence. This can be determined by any method known in the art, for example, but not by way of limitation, by sequencing, restriction mapping, PCR amplification assays, etc.

Once the appropriate vector containing the regulatory region/regulatory gene sequences has been created, the tagged ribosomal fusion protein gene can be incorporated into the characterizing gene sequence by any method known in the art for manipulating DNA. In one embodiment, homologous recombination in bacteria is used for target-directed insertion of the tagged ribosomal fusion protein gene sequence into the genomic DNA encoding the characterizing endogenously regulated gene and sufficient regulatory sequences to promote expression of the gene in its endogenous expression pattern, which characterizing gene sequences have been inserted into a vector. The vector comprising the tagged ribosomal fusion protein gene and regulatory gene sequences is then introduced into the genome of a potential founder organism for generating a line of transformed organisms, using methods well known in the art, e.g., those methods described herein. Such transformed organisms are then screened for expression of the tagged ribosomal fusion protein gene coding sequences that mimics the expression of the endogenous characterizing gene. Several different constructs containing nucleic acids of the invention may be introduced into several potential founder organisms and the resulting transformed organisms are then screened for the best, (e.g., highest level) and most accurate (best mimicking expression of the endogenous characterizing gene) expression of the tagged ribosomal fusion protein gene coding sequences.

The nucleic acid construct can be used to transform a host or recipient cell or organism using well known methods, e.g., those described herein. Transformation can be either a permanent or transient genetic change. In one aspect of the invention, a vector is used for stable integration of the nucleic acid construct into the genome of the cell. In another aspect, the vector is maintained episomally.

8) Bacterial Artificial Chromosomes (BACs) and Other Vectors

The methods described herein provide transformed organisms, e.g., transgenic mice that express a tagged ribosomal protein within a chosen cell type. In some embodiments, BAC-mediated recombination (Yang, et al., 1997, Nat. Biotechnol. 15(9):859-865) is used to create the transformed organism. Such expression is achieved by using the endogenous regulatory sequences and transcriptional units of a particular gene, wherein the expression of gene is a defining characteristic of the chosen cell type (as also described in PCT/US02/04765, entitled “Collections of Transgenic Animal Lines (Living Library)” by Serafini, published as WO 02/064749 on Aug. 22, 2002, which is incorporated by reference herein in its entirety). A collection of transgenic animals expressing tagged ribosomal proteins within a set of chosen cell types is assembled, and this is often referred to as a BACarray collection.

In another embodiment, a nucleic acid of the invention is inserted into a yeast artificial chromosome (YAC) (Burke et al., 1987 Science 236: 806-12; and Peterson et al., 1997, Trends Genet. 13: 61). A collection of transgenic animals expressing tagged ribosomal proteins within a set of chosen cell types or subtypes is assembled, and this is often referred to as a YACarray collection.

In other embodiments, the nucleic acid of the invention is inserted into another vector developed for the cloning of large segments of mammalian DNA, such as a cosmid or bacteriophage P1 (Sternberg et al., 1990, Proc. Natl. Acad. Sci. USA 87: 103-07). The approximate insert size is about 30-35 kb for cosmids and 100 kb for bacteriophage P1.

In another embodiment, the nucleic acid of the invention is inserted into a P-1 derived artificial chromosome (PAC) (Mejia et al., 1997, Retrofitting vectors for Escherichia coli-based artificial chromosomes (PACs and BACs) with markers for transfection studies, Genome Res. 7(2):179-86). The maximum insert size is about 300 kb.

BACs and other vectors used in the methods described here often can accommodate, and in certain embodiments comprise, large pieces of heterologous DNA such as genomic sequences. Such vectors can contain an entire genomic locus, or at least sufficient sequences to confer endogenous regulatory expression pattern and to insulate the expression of coding sequences from the effect of regulatory sequences surrounding the site of integration of the nucleic acid in the genome to mimic better wild type expression. When entire genomic loci or portions thereof are used, few, if any, site-specific expression problems are encountered, unlike insertions of nucleic acids into smaller sequences. When insertions of nucleic acids are too large, other problems can be encountered. In some embodiments, the vector is a BAC containing genomic sequences into which a selected sequence encoding a molecular tag that has been inserted by directed homologous recombination in bacteria, e.g., by the methods of Heintz WO 98/59060; Heintz et al., WO 01/05962; Yang et al., 1997, Nature Biotechnol. 15: 859-865; Yang et al., 1999, Nature Genetics 22: 327-35; which are incorporated herein by reference in their entireties.

Using such methods, a BAC can be modified directly in a recombination-deficient E. coli host strain by homologous recombination. i.e., a cell that cannot independently support homologous recombination, e.g., Rec A.sup.-. In one embodiment, homologous recombination in bacteria is used for target-directed insertion of a sequence encoding a molecularly tagged ribosomal protein into the genomic DNA encoding sufficient regulatory sequences (termed “characterizing gene sequences”) to promote expression of the tagged ribosomal protein in the endogenous expression pattern of the characterizing gene, which sequences have been inserted into the BAC. The BAC comprising the tagged fusion protein sequence under the control/regulation of a regulatory region comprising sequences from a genomic locus of the desired characterizing gene is then recovered and introduced into the genome of a potential founder organism for a line of transformed organisms. In one aspect, the particular nucleotide sequence that has been selected to undergo homologous recombination is contained in an independent origin based cloning vector introduced into or contained within the host cell, and neither the independent origin based cloning vector alone, nor the independent origin based cloning vector in combination with the host cell, can independently support homologous recombination (e.g., is RecA). The exact method used to introduce the tagged ribosomal protein encoding sequence and to remove (or not) the RecA (or other appropriate recombination enzyme) will depend upon the nature of the BAC library used (for example, the selectable markers present on the BAC vectors) and such modifications are within the skill in the art.

Once the BAC containing the characterizing gene regulatory sequences and molecularly tagged ribosomal protein coding sequences in the desired configuration is identified, it can be isolated from the host E. coli cells using routine methods and used to make transformed organisms as described within.

Alternatively, the BAC can also be engineered or modified by “E-T cloning,” as described by Muyrers et al (1999, Nucleic Acids Res. 27(6): 1555-57, incorporated herein by reference in its entirety). Using these methods, specific DNA may be engineered into a BAC independently of the presence of suitable restriction sites. This method is based on homologous recombination mediated by the recE and recT proteins (“ET-cloning”) (Zhang et al., 1998, Nat. Genet. 20(2): 123-28; incorporated herein by reference in its entirety).

Host Cells and Organisms Engineered to Express Molecularly Tagged Ribosomes

1) Introduction of Vectors into Host Cells

In one aspect, a vector containing the nucleic acid encoding the regulatory sequences and the molecularly tagged fusion protein can be introduced transiently or stably into the genome of a host cell. In another aspect, the vector can be transiently transfected wherein it is not integrated, but is maintained as an episome. The terms “host cell” and “recombinant host cell” are used interchangeably herein.

It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

A host cell can be any prokaryotic (e.g., bacterium such as E. coli) or eukaryotic cell (e.g., a cell from a yeast, plant, insect (e.g., Drosophila), amphibian, amniote, or mammal, to name but a few. In certain embodiments the host cell is a human cell, a rodent cell, a mouse cell, a rat cell, a mammalian cell, an immortalized cultured cell or primary human or rodent cell. In specific embodiments, the host cells are human or rodent embryonic stem cells, neuronal stem cells, hippocampal stem cells, hippocampal progenitor cells, or partially differentiated pluripotent cells, or tumor cells or cancer cells (particularly circulating cancer cells such as those resulting from leukemias and other blood system cancers). Methods can utilize genetically engineered host cells that contain any of the foregoing tagged ribosomal protein coding sequences, optionally operatively associated with a regulatory element (preferably from a characterizing gene, as described above) that directs the expression of the coding sequences in the host cell. Both cDNA and genomic sequences can be cloned and expressed.

In one aspect, the host cell is recombination deficient, i.e., Rec.sup.-, and used for BAC recombination. In specific embodiments the host cell may contain more than one type of ribosomal fusion, where the fusion of the different ribosomal is to the same or different tags. Alternatively, a single ribosomal may be fused to more than one tag, for example, at both is N-terminal and C-terminal ends.

A vector containing a nucleotide sequence can be introduced into the desired host cell by methods known in the art, e.g., transfection, transformation, transduction, electroporation, infection, microinjection, cell fusion, DEAE dextran, calcium phosphate precipitation, liposomes, LIPOFECTIN.™, lysosome fusion, synthetic cationic lipids, use of a gene gun or a DNA vector transporter, such that the nucleotide sequence is transmitted to offspring in the line. For various techniques for transformation or transfection of mammalian cells, see Keown et al., 1990, Methods Enzymol. 185: 527-37; Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, N.Y.

In certain embodiments, the vector is introduced into a cultured cell. In other embodiments, the vector is introduced into a proliferating cell (or population of cells), e.g., a tumor cell, a stem cell, a blood cell, a bone marrow cell, a cell derived from a tissue biopsy, etc.

2) Introduction of Vectors to Create Transgenic Animals

Particular embodiments encompass methods of introduction of the vector containing the nucleic acid, using pronuclear microinjection into the mononucleus of a mouse embryo or infection with a viral vector comprising the construct. Methods of pronuclear injection into mouse embryos are well-known in the art and described in Hogan et al. 1986, Manipulating the Mouse Embryo, Cold Spring Harbor Laboratory Press, New York, N.Y. and Wagner et al., U.S. Pat. No. 4,873,191, issued Oct. 10, 1989, herein incorporated by reference in their entireties. Viral methods of inserting nucleic acids are known in the art. Targeted delivery can utilize lentivirus, retrovirus, adenovirus, herpes simplex virus, adeno associated virus, or any other virus amenable to gene delivery.

3) Transgenic Organisms

Any model organism can be engineered to express a molecularly tagged ribosome using methods well known in the art. These can include but are not limited to non-human primates, mice, rats, sheep, dogs, cows, goats, chickens, amphibians, fish, zebrafish, (Danio rerio), flies (Drosophila melanogaster), worms (Caenorhabditis elegans), or yeast (or other fungi). A transgenic animal is a non-human animal in which one or more of the cells of the animal includes a nucleic acid that is non-endogenous (i.e., heterologous) and is present as an extrachromosomal element in a portion of its cell or stably integrated into its germ line DNA (i.e., in the genomic sequence of most or all of its cells). In certain embodiments a transgenic animal comprises stable changes to the germline sequence. Heterologous nucleic acid is introduced into the germ line of such a transgenic animal by genetic manipulation of, for example, embryos or embryonic stem cells of the host animal.

The heterologous nucleic acid may integrate into the genome of the founder organism by random integration. If random, the integration preferably does not knock out, e.g., insert into, an endogenous gene(s) such that the endogenous gene is not expressed or is mis-expressed.

In other embodiments, the nucleic acid of the may integrate by a directed method, e.g., by directed homologous recombination (“knock-in”), Chappel, U.S. Pat. No. 5,272,071; and PCT publication No. WO 91/06667, published May 16, 1991; U.S. Pat. No. 5,464,764; Capecchi et al., issued Nov. 7, 1995; U.S. Pat. No. 5,627,059, Capecchi et al issued, May 6, 1997; U.S. Pat. No. 5,487,992, Capecchi et al., issued Jan. 30, 1996).

Transformed organisms, e.g., transgenic animals, can be generated by random integration of a vector into the genome of the organism, for example, by pronuclear injection in an animal zygote as described above. Other methods involve introducing the vector into cultured embryonic cells, for example ES cells using methods of injection or electroporation well known in the art, and then introducing the transformed cells into animal blastocysts, thereby generating a “chimeras” or “chimeric animals”, in which only a subset of cells have the altered genome. Chimeras are often used for breeding purposes in order to generate the desired transgenic animal Animals having a heterozygous alteration are generated by breeding of chimeras. Male and female heterozygotes are typically bred to generate homozygous animals.

Methods for generating transgenic animals via embryo manipulation and microinjection, particularly animals such as mice, have become conventional in the art and are described, for example, in U.S. Pat. Nos. 4,736,866 and 4,870,009, U.S. Pat. No. 4,873,191, in Hogan, Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986) and in Wakayama et al., 1999, Proc. Natl. Acad. Sci. USA, 96:14984-89. Similar methods are used for production of other transgenic animals.

A transgenic founder animal can be identified based upon the presence of the nucleic acid introduced in its genome and/or expression of mRNA in tissues or cells of the animals A transgenic founder animal can then be used to breed additional animals carrying the nucleic acid of. These transgenic animals can further be bred to other transgenic animals carrying other heterologous nucleic acids. Progeny harboring homologously recombined or integrated DNA in their germline cells can be used to breed animals in which all cells of the animal contain the homologously recombined DNA by germline transmission of the nucleic acid of interest.

Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut et al., 1997, Nature 385: 810-13 and PCT Publication NOS. WO 97/07668 and WO 97/07669.

Once the transgenic mice are generated they may be bred and maintained using methods well known in the art. By way of example, the mice may be housed in an environmentally controlled facility maintained on a 10 hour dark: 14 hour light cycle. Mice are mated when they are sexually mature (6 to 8 weeks old). In certain embodiments, the transgenic founders or chimeras are mated to an unmodified animal. In one embodiment, the transgenic founder or chimera is mated to C57BL/6 mice (Jackson Laboratories). In a specific embodiment where the nucleic acid is introduced into ES cells and a chimeric mouse is generated, the chimera is mated to 129/Sv mice, which have the same genotype as the embryonic stem cells. Protocols for successful creation and breeding of transgenic mice are known in the art (Manipulating the Mouse Embryo. A Laboratory Manual, 2nd edition. B. Hogan, Beddington, R., Costantini, F. and Lacy, E., eds. 1994. Cold Spring Harbor Laboratory Press: Plainview, N.Y.).

Methods to establish heterozygousity or homozygousity are well known in the art and utilize PCR and Southern blotting.

In some embodiments, the transgenic mice are so highly inbred to be genetically identical except for sexual differences. The homozygotes are tested using backcross and intercross analysis to ensure homozygosity. Homozygous lines for each integration site in founders with multiple integrations are also established. Brother/sister matings for 20 or more generations define an inbred strain. In another preferred embodiment, the transgenic lines are maintained as hemizygotes.

In an alternative embodiment, individual genetically altered mouse strains are also cryopreserved rather than propagated. Methods for freezing embryos for maintenance of founder animals and transgenic lines are known in the art. Methods for reconstituting frozen embryos and bringing the embryos to term are known in the art.

The nucleic acid encoding the molecularly tagged ribosomal protein may be introduced into the genome of a founder plant (or embryo that gives rise to the founder plant) using methods well known in the art (Newell, 2000, Plant transformation technology. Developments and applications, Mol. Biotechnol. 16(1):53-65; Kumar and Fladung, 2001, Controlling transgene integration in plants, Trends in Plant Science 6 (4): 155-159). The nucleic acid encoding the molecularly tagged ribosomal protein may be introduced into the genome of bacteria and yeast using methods described in Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y., Chapters 1 and 13, respectively).

4) Screening for Expression of Tagged Ribosomal Proteins in Transgenic Organisms

Potential founder organisms for a line of transformed organisms can be screened for expression of the molecularly tagged fusion protein coding sequence by ribosomes in the population of cells characterized by expression of the endogenous characterizing gene.

In one embodiment, immunohistochemistry using an antibody specific for the molecular tag or a marker activated or repressed thereby is used to detect expression of the molecular tag. In another embodiment, other methods and devices are used to detect the tag, such as with the use of optics-based sorting or magnetics-based sorting.

Transformed organisms that exhibit appropriate expression (e.g., detectable expression having substantially the same expression pattern as the endogenous characterizing gene in a corresponding non-transgenic organism or anatomical region thereof, i.e., detectable expression in at least 50%, 60%, 70%, 80%, 90% or, preferably, 95% of the cells shown to express the endogenous gene by in situ hybridization) are selected as lines of transformed organisms.

BACarray Lines

In some embodiments, a collection of lines of transformed organisms that contain a selected subset of cells or a cell population expressing molecularly-tagged ribosomes is provided. In some aspects, when the transformed organism has been created utilizing BAC-related methods, this is referred to as a BACarray collection. The collection comprises at least 2 individual lines, preferably at least 5, 10, 25, 50, 100, 500, 1000, 1500, 2000, 2500, 5000, 10,000, 15,000, or 30,000 individual lines. Each individual line is selected for the collection based on the identity of the subset of cells in which the molecularly tagged ribosomes are expressed.

In one embodiment, a BACarray line related to neuronal diseases (neurodegenerative, neuropsychiatric, behavioral, etc) can be created. These include, but are not limited to BACarray lines specific for expression for cells involved in disease processes such as depression, obesity, anxiety, epilepsy, sleep, Parkinson's disease, ADHD, Huntington's disease, addiction, dementia, Alzheimer's disease, ALS, and the like. For example, see FIG. 1 for a representative BACarray collection specific to central nervous system diseases.

In one embodiment, a BACarray collection relating to sub-regions of a tissue, for example regions of the central nervous system can be created. These include, but are not limited to BACarray lines specific for expression in only striatal cells (for example expression in medium spiny neurons, striatonigral and/or striatopallidal neurons), in only cerebellar cells (for example expression in Purkinje neurons, astrocytes, Bergmann glia, granule cells, etc), in only hippocampal cells (for example, expression in CA1 neurons, CA2 neurons, CA3/4 neurons, glia, astrocytes, DG neurons), in only hypothalamic cells, in only spinal cord cells, in only pineal gland cells, and the like.

In another related embodiment, a BACarray collection relating to a particular biological function can be created. These include but are not limited to BACarray lines specific for functions related to myelination, excitatory neural transmission, motor function, migration, adhesion, infiltration, processing of noxious stimuli, processing of sensory stimuli, visual processing, auditory processing, olfactory processing, vestibular control, regulation of feeding and satiety, regulation of wakefulness and sleep, regulation of reward behavior (for example as how it relates to addictive behavior), and the like.

Analyses of mRNA Species

The embodiments described herein provide for translation profiling and molecular phenotyping of particular cells and/or tissues. mRNA complexed in polysomes with tagged ribosomal proteins are isolated and can be analyzed by any method known in the art. In one aspect, the translational profile of cells expressing the tagged ribosomal proteins can be analyzed by isolating the mRNA and constructing cDNA libraries or by labeling the RNA for gene expression analysis, for example by disposing the mRNA on a microarray. Embodiments of this invention utilize techniques described in US 2005/0009028, which is herein incorporated in its entirety.

In one aspect, mRNA bound by the tagged ribosomal proteins may be used to produce a cDNA library and, in fact, a collection of such cell type-specific, cell-subtype specific, tissue-specific, organism-specific, disease-specific, function-specific, cDNA libraries may be generated from different populations of isolated cells. Such cDNA libraries are useful to analyze gene expression, isolate and identify cell type-specific genes, splice variants and non-coding RNAs, as well as identify co-regulated gene sets for a particular cell related to a function or a disease state. In another aspect, such cell-type specific libraries prepared from mRNA bound by, and isolated from, the tagged ribosomal proteins from treated and untreated or transgenic or otherwise manipulated cells/animals having can be used, for example in subtractive hybridization procedures, to identify genes expressed at higher or lower levels in response to a particular treatment or in a disease state as compared to untreated animals. The mRNA isolated from the tagged ribosomal proteins may also be analyzed using particular microarrays generated and analyzed by methods well known in the art. Gene expression analysis using microarray technology is well known in the art. Methods for making microarrays are taught, for example, in U.S. Pat. No. 5,700,637 by Southern, U.S. Pat. No. 5,510,270 by Fodor et al. and PCT publication WO 99/35293 by Albrecht et al., which are incorporated by reference in their entireties. By probing a microarray with various populations of mRNAs, transcribed genes in certain cell populations can be identified. Moreover, the pattern of gene expression in different cell types of cell states may be readily compared.

The mRNA bound by the tagged ribosomal proteins may be analyzed, for example by northern blot analysis, PCR, RNase protection, etc., for the presence of mRNAs encoding certain protein products and for changes in the presence or levels of these mRNAs depending on manipulation.

In yet another embodiment, specific cells or cell populations that express a potential a molecularly tagged ribosomal protein are isolated from the collection and analyzed for specific protein-protein interactions or an entire protein profile using proteomics methods known in the art, for example, chromatography, mass spectroscopy, 2D gel analysis, etc.

Other types of assays may be used to analyze the cell population expressing the molecularly tagged ribosomal protein either in vivo, in explanted or sectioned tissue or in the isolated cells, for example, to monitor the response of the cells to a certain manipulation/treatment or candidate agent (for example, a small molecule, an antibody, a hybrid antibody, an antibody fragment, a siRNA, an antisense RNA, an aptamer, a protein, or a peptide) or to compare the response of the animals, tissue or cells to expression of the target or inhibitor thereof, with animals, tissue or cells from animals not expressing the target or inhibitor thereof. The cells may be monitored, for example, but not by way of limitation, for changes in electrophysiology, physiology (for example, changes in physiological parameters of cells, such as intracellular or extracellular calcium or other ion concentration, change in pH, change in the presence or amount of second messengers, cell morphology, cell viability, indicators of apoptosis, secretion of secreted factors, cell replication, contact inhibition, etc.), morphology, etc.

In particular embodiments, the isolated mRNA is used to probe a comprehensive expression library (see, e.g., Serafini et al., U.S. Pat. No. 6,110,711, issued Aug. 29, 2000, which is incorporated by reference herein). The library may be normalized and presented in a high density array, such as a microarray.

In a particular embodiment, a subpopulation of cells expressing a molecularly tagged ribosomal protein is identified and/or gene expression analyzed using the methods of Serafini et al., WO 99/29877 entitled “Methods for defining cell types,” which is hereby incorporated by reference in its entirety.

Data from such analyses may be used to generate a database of gene expression analysis for different populations of cells in the animal or in particular tissues or anatomical regions, for example, in the brain. Using such a database together with bioinformatics tools, such as hierarchical and non-hierarchical clustering analysis and principal components analysis, cells are “fingerprinted” for particular indications from healthy and disease-model animals or tissues, co-regulated gene sets for a particular function, and the like.

Applications and Considerations

1) Sensitivity

The methods provided herein are applicable to any cell, tissue, or organism as well as any disease or disorder. The sensitivity afforded by the ability to interrogate an individual cell subtype in a complex heterogeneous tissue or organ system provides a resolution than can be greater than with other techniques such as whole-tissue microarrays and in situ hybridization. In some embodiments the TRAP method of detecting one or more cell subtype-specific mRNAs/genes allows for identification of greater than 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45% and even 50% of the mRNAs/genes enriched in that cell type which are not detectable by other means such as whole-tissue microarrays or in situ hybridization

2) Diseases and Disorders

The methods provided herein are applicable to any cell, tissue, or organism as well as any disease or disorder. Several disease are cited in, but not limited to those found in the ‘The Merck Manual of Diagnosis and Therapy’, often called simply ‘The Merck Manual’ (2006). The diseases and disorders can be central nervous system disorders, peripheral nervous system disorders, and non nervous system disorders.

Examples of neurodegenerative diseases/disorders include, but are not limited to: alcoholism, Alexander's disease, Alper's disease, Alzheimer's disease, Amyotrophic lateral sclerosis, Ataxia telangiectasia, Batten disease (also known as Spielmeyer-Vogt-Sjogren-Batten disease), Bovine spongiform encephalopathy (BSE), Canavan disease, Cockayne syndrome, Corticobasal degeneration, Creutzfeldt-Jakob disease, Huntington's disease, HIV-associated dementia, Kennedy's disease, Krabbe's disease, Lewy body dementia, Machado-Joseph disease (Spinocerebellar ataxia type 3), Multiple sclerosis, Multiple System Atrophy, Narcolepsy, Neuroborreliosis, Parkinson's disease, Pelizaeus-Merzbacher Disease, Pick's disease, Primary lateral sclerosis, Prion diseases, Refsum's disease, Sandhoffs disease, Schilder's disease, Subacute combined degeneration of spinal cord secondary to Pernicious Anaemia, Schizophrenia, Spielmeyer-Vogt-Sjogren-Batten disease (also known as Batten disease), Spinocerebellar ataxia (multiple types with varying characteristics), Spinal muscular atrophy, Steele-Richardson-Olszewski disease, and Tables dorsalis.

Examples of neuropsychiatric diseases/disorders include, but are not limited to: depression, bipolar disorder, mania, obsessive compulsive disease, addiction, ADHD, schizophrenia, auditory hallucinations, eating disorders, hysteria, autism spectrum disorders and personality disorders.

Examples of neurodevelopmental diseases/disorders include, but are not limited to: attention deficit hyperactivity disorder (ADHD), attention deficit disorder (ADD), schizophrenia, obsessive-compulsive disorder (OCD), mental retardation, autistic spectrum disorders (ASD), cerebral palsy, Fragile-X Syndrome, Downs Syndrome, Rett's Syndrome, Asperger's syndrome, Williams-Beuren Syndrome, childhood disintegrative disorder, articulation disorder, learning disabilities (i.e., reading or arithmetic), dyslexia, expressive language disorder and mixed receptive-expressive language disorder, verbal or performance aptitude. Diseases that can result from aberrant neurodevelopmental processes can also include, but are not limited to bi-polar disorders, anorexia, general depression, seizures, obsessive compulsive disorder (OCD), anxiety, bruixism, Angleman's syndrome, aggression, explosive outburst, self injury, post traumatic stress, conduct disorders, Tourette's disorder, stereotypic movement disorder, mood disorder, sleep apnea, restless legs syndrome, dysomnias, paranoid personality disorder, schizoid personality disorder, schizotypal personality disorder, antisocial personality disorder, borderline personality disorder, histrionic personality disorder, narcissistic personality disorder, avoidant personality disorder, dependent personality disorder, reactive attachment disorder; separation anxiety disorder; oppositional defiant disorder; dyspareunia, pyromania, kleptomania, trichotillomania, gambling, pica, neurotic disorders, alcohol-related disorders, amphetamine-related disorders, cocaine-related disorders, marijuana abuse, opioid-related disorders, phencyclidine abuse, tobacco use disorder, bulimia nervosa, delusional disorder, sexual disorders, phobias, somatization disorder, enuresis, encopresis, disorder of written expression, expressive language disorder, mental retardation, mathematics disorder, transient tic disorder, stuttering, selective mutism, Crohn's disease, ulcerative colitis, bacterial overgrowth syndrome, carbohydrate intolerance, celiac sprue, infection and infestation, intestinal lymphangiectasia, short bowel syndrome, tropical sprue, Whipple's disease, Alzheimer's disease, Parkinson's Disease, ALS, spinal muscular atrophies, and Huntington's Disease. Further examples, discussion, and information on neurodevelopmental disorders can be found, for example, through the Neurodevelopmental Disorders Branch of the National Institute of Mental Health (worldwide website address at nihm.nih.gov/dptr/b2-nd.cfm).

3) Profiling of mRNA Species

In one embodiment, the invention provides for a method to obtain a translational profile of a cell type of interest. The method comprises expressing a tagged ribosomal protein under the control of a regulatory sequence specific to a gene expressed in the cell type of interest, isolating mRNAs complexed with the ribosomal protein from the cell, and identifying the mRNAs, thereby obtaining a translational profile for the cell type of interest.

In one exemplary embodiment the cell types of interest are striatonigral and striatopallidal cells. L10a fused to eGFP under the control of a Drd1a or a Drd2 specific regulatory sequence is expressed and translational profiles are obtained. Tables 10, 13, 17, and 18 identify genes that are translationally profiled in striatonigral and striatopallidal cells.

In another exemplary embodiment the cell type of interest are cholinergic motor neurons. Ribosomal protein L10a fused to eGFP under the control of a choline acetyl transferase (chat)-specific regulatory sequence is expressed and translational profiles are obtained. Table 19 identifies genes that are translationally profiled in cholinergic motor neurons. In this embodiment, the cholinergic motor neurons are spatially separated.

In another exemplary embodiment the cell type of interest are cerebellar neurons and glia, specifically Purkinje neurons and Bergmann glia. Ribosomal protein L10a fused to eGFP under the control of either a Pcp2 or Septin4-specific regulatory sequence is expressed and translational profiles are obtained. Table 20 identifies genes that are translationally profiled in cerebellar Purkinje neurons and Bergmann glia.

In another embodiment a gene translational profile following the manipulation of a cell, tissue or organism is obtained. The method comprises manipulating a cell, tissue or organism, using the TRAP methodology to obtain a translational profile, and comparing the profiled to a reference profile from an non-manipulated cell, tissue or organism. Manipulations include but are not limited to:

-   -   a. Pharmacological: for example administration of a candidate         agent such as a small molecule antagonist or an agonist;         administration of a pharmacological agent to recapitulate a         disease such as the administration of MPTP or OHDA to an animal         model or cell culture to induce a Parkinson's disease-like         state; or administration of a drug or substance of abuse, for         example cocaine or alcohol;     -   b. Genetic: for example introduction of a germline or         non-germline mutation or transgene to recapitulate an animal or         cellular model of a disease or disorder such as ataxia,         Parkinson's disease, Alzheimer's disease, autism spectrum         disorders and the like;     -   c. Mechanical: for example surgical treatment; and/or     -   d. Environmental: for example change of habitat, climatic         change, reversal of day-nite cycles; for example induction of a         chronic-mild-stress protocol, art-recognized, to recapitulate a         depression-like phenotype perturbations. Examples of candidate         agents are but not limited to a small molecule, an antibody, a         hybrid antibody, an antibody fragment, a siRNA, an antisense         RNA, an aptamer, a protein, or a peptide. In exemplary         embodiments, the cell is a cell of a nervous system, such as a         neuronal or glial cell, a striatal cell, a cerebellar cell, a         hippocampal cell, a hypothalamic cell, a cortical cell, a         dopaminergic cell, a spinal cord cell. In other embodiments the         cell is a neuron such as a dopaminergic neuron or a medium spiny         neuron.

In an exemplary embodiment, the effect of cocaine on the translational profile of dopaminergic striatonigral and striatopallidal cells is obtained. In related embodiments the effects of acute cocaine administration and the effects of chronic cocaine administration is obtained. In specific embodiments, the gene translation changes following acute cocaine treatment comprise those identified in Table 15. In related specific embodiments, the gene translation changes following chronic cocaine treatment comprise those identified in Table 16.

4) Disease Screening, Diagnostics, Prognostics, and Theranostics

One embodiment of this invention is to establish a translational profile and molecular phenotype for a tissue or cell type taken from a subject to be screened for, suspected of having, or presenting with a particular disease or disorder, using the methods described herein. In this aspect, markers associated with and/or indicative of a particular disease or disorder are identified. In one embodiment, in a manner similar to a biopsy, a subject's tissue can be removed for sampling. In another embodiment, a sampling of cerebrospinal fluid (CSF) will be obtained from the subject. In other embodiments, any bodily fluid can be obtained from the subject. Following the isolation of desired tissue or fluid, a molecularly tagged ribosomal protein is introduced with the regulatory elements required for a cell-type specific expression. The translational profile and molecular phenotype is established using the methods described herein. The resulting profile and phenotype is then be compared to a profile and phenotype obtained using said methods from a subject or subjects not having or presenting with the particular disease or disorder, i.e. a reference cell, tissue or subject. The results can be used for diagnostics, prognostic, or theranostic purposes in relation to a disease or disorder. The disease or disorder to be detected, diagnosed, prognosed or theranosed, can be any disease named herein, or previously undescribed diseases and disorders.

In certain embodiments, BAC-mediated expression can be utilized to drive expression of a molecularly tagged ribosomal protein in a particular cell type. Several BACarrays have been created and collected that, based on expression of the endogenously regulated gene, have restricted patterns of expression that are directly relevant to several diseases or disorders (FIG. 1)

5) Screening for Modulatory Agents

Another embodiment of this invention is to screen for candidate agents for modulation of a desired modulatory (antagonistic, agonistic, synergistic modulation) activity. In certain embodiments a vehicle or candidate agent is administered in a single or repeated dose to either a cell type or a subject. Following administration a translational profile is established for the cell type of interest based on the methods disclosed herein. The profile and phenotypes from those dosed with candidate agent are compared and related to the profile and phenotype from those dosed with a vehicle (i.e. reference sample). A determination is made whether a candidate agent modulates translation and phenotype of one or more mRNA isolated from the cell type of interest, thereby screening said candidate agent for translational modulation. In some embodiments the candidate agent may be a candidate therapeutic agent or drug. In other embodiments, the candidate agent may be a toxin. In some embodiments the candidate agent may be but is not limited to a small molecule, an antibody, hybrid antibody or antibody fragment, a siRNA, an antisense RNA, an aptamer, a protein therapeutic, or a peptide.

6) Therapeutic Target Screening and Selection

Another aspect of this invention is to identify candidate therapeutic targets for a disease or disorder. In certain embodiments this would entail transforming a cell or creating an organism expressing a molecularly tagged ribosomal protein and exposing the cell or organism to a pertuthation or stimulus. mRNA transcripts selectively down regulated or up regulated would be potential targets for ameliorating the pertuthation. In other embodiments, a translational profile from a cell from a subject with a disease or disorder, a cell that has been perturbed, etc will be compared to an otherwise normal or unperturbed cell. The differential translational profile will allow for identification of potential therapeutic targets.

In one embodiment, any mRNA found to be upregulated in a cell type of interest associated with a disease or disorder, for example in but not limited to a striatal, hippocampal, cortical, cerebellar, spinal cord, hypothalamic, pineal, retinal, auditory, olfactory, vestibular, or brain stem cell may represent a target for antagonism. That is, the protein encoded by the identified mRNA would be a therapeutic target for which antagonists could be developed. In one exemplary embodiment, the target of interest is Gpr6. Gpr6 represents a target for which antagonists can be screened for and developed as a therapeutic for Parkinson's disease, due to its overexpression.

In a similar manner, an mRNA found to be downregulated in a cell type of interest associated with a disease or disorder, as above, could represent a target for agonism. That is, the protein encoded by the identified mRNA would be a therapeutic target for which agonists could be developed.

7) Personalized Medicine

In one embodiment, a method is provided for assessing whether a subject in need thereof is amenable to a therapeutic agent, comprising determining a translational profile for a cell type, cell sub-type or tissue from the subject, determining if the translational profile is predictive of treatment with one or more therapeutic agents, and identifying the one or more therapeutic agents to administer to the subject.

In another embodiment, a method is provided for screening one or more therapeutic agents in a subject in need thereof comprising, administering one or more therapeutic agents to a subject, determining a translational profile for a cell type, cell sub-type or tissue from the subject, comparing the translational profile obtained to one or more reference profiles that indicate a positive or negative prognosis, and determining the treatment should continue or be modified based on the comparison. In a further embodiment, it is determined that a different treatment modality (e.g., different therapeutic agent should be administered, such as for example, a second line drug, that is shown to be amenable to the translational profile observed for a first line drug).

8) Co-Regulated Gene Sets

In one embodiment, a method to identify a co-regulated gene set for a known function or a candidate function for a novel gene is provided. The method comprises determining the translational profiles for a plurality of cell types that express a gene associated with a specific function, comparing the translational profiles to determine what additional genes are similarly regulated thereby identifying a co-regulated gene set involved in the function.

In further embodiments the function can be a cellular function or a cellular process. In other related embodiments the method is applied to determine a co-regulated gene set involved in myelination, excitatory neural transmission, motor function, cellular migration, cellular adhesion, cellular infiltration, processing of noxious stimuli, processing of sensory stimuli, visual processing, auditory processing, olfactory processing, vestibular control, regulation of feeding and satiety, regulation of wakefulness and sleep, or the regulation of reward behavior.

In one exemplary embodiment, the method is applied to determine a co-regulated gene set involved in myelination comprising determining the translation profiles for a plurality of cells that express the myelin basic protein (Mbp). In a related embodiment the gene set comprises one or more of the genes listed in Table 9.

This method can be useful in identifying gene sets for known functions or candidate functions for novel gene products, since in many cases the cohorts of co-regulated genes can include genes with well known functions

Kits

In a further aspect, the present invention provides kits. In certain embodiments the kit contains reagents for determining the presence, absence, and/or differential presence, of one or more markers indicative of a disease, disorder, and/or pharmacologic, genetic, or environmental manipulation. The disease could be, but is not limited to a neurodegenerative, neuropsychiatric, neurodevelopmental disorder in a sample from an individual suspected of having a susceptibility to such a disease or disorder. In another embodiment, the disease or disorder is a proliferative disease such as a cancer. In other embodiments the kit is utilized to identify co-regulated gene sets for a particular biological function. Biological functions are described herein.

In one embodiment, the kit contains a customized set of clones, vectors, molecular tags from which to choose, for molecular labeling and materials such as CDs, instructions for use, and other reference guides that would allow the individual to choose the correct clone/vector for the cell type, tissue, molecular pathway, or circuit of choice. In another embodiment the kit contains a recombinant vector encoding a nucleic acid sequence which encodes a ribosomal protein and a detectable tag operably linked to a regulatory region. In particular embodiments, the kit can contain a customized BACarray clone collection relevant for a particular disease or disorder.

In one embodiment a kit includes a recombinant vector engineered to express a nucleic acid sequence encoding a ribosomal protein and a detectable tag operably linked to a regulatory region containing sequences endogenous to a genomic locus of a gene of interest. In a related embodiment the ribosomal protein is fused in-frame to a detectable tag. In certain embodiments, the recombinant vector is a BAC. The gene of interested can be selected from but is not limited to one that expresses in a striatal cell, cerebellar cell, cortical cell, hypothalamic cell, hippocampal cell, brainstem cell, and a spinal cord cell. In exemplary embodiments the tag is eGFP and the ribosomal protein is L10a.

EXAMPLES

The methodology reported here provides an enabling technology for translational profiling and molecular phenotyping. Examples allow for defining the distinguishing molecular characteristics of closely related or critical cell types. Examples also demonstrate that methods described herein can be employed to analyze physiological adaptations of specific cell types in vitro, in vivo, ex vivo, or in situ.

In various embodiments, compositions and methods feature methodology, which readily and reproducibly identify translated mRNAs in any cell type or tissue of interest. This methodology involves expression of a molecularly tagged ribosomal transgene, which enables tagging of polysomes for isolation, purification and identification of mRNA, in specific populations. In some embodiments, this can be achieved using Bacterial Artificial Chromosome (BAC) transgenic mice, allowing translational profiling and molecular phenotyping from whole animals.

The following examples are offered to illustrate, but not to limit the claimed invention. Some embodiments of the present invention have been shown and described herein, but it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention.

Example 1 Molecular Tagging of a Ribosomal Proteins

mRNAs translated into protein are at one point usually attached to a ribosome or a polyribosome complex (polysomes). Any tag, not limited to those named herein, fused to a ribosomal protein allows for isolation of bound mRNAS. eGFP fused to the N-terminus of the large subunit ribosomal protein L10a, hereafter eGFP-L10a, was utilized in this and ensuing examples, but is by no ways limiting to the tens, hundreds, thousands, or even more tag-protein combinations that can be utilized.

FIG. 2: (a) A schematic is presented to illustrate affinity purification of eGFP-tagged Polysomes (originating from the target cell population) using anti-GFP antibody-coated beads (Schematic in a). Fusions of Enhanced Green Fluorescent Protein (eGFP) with ribosomal proteins were screened for efficient incorporation into polysomes to provide a tag for all translated cellular mRNAs. (b): A BAC carrying the eGFP-L10a fusion protein was transfected into HEK293T cells. The eGFP-L10a fusion protein's nucleolar and cytoplasmic localization was consistent with incorporation into intact ribosomes and immuno-electron microscopy data demonstrated its presence on polysome complexes. The figure displays transmission electron micrographs of anti-GFP coated magnetic beads after incubation with extracts taken from HEK293T cells transfected with an empty vector (left panel) or the eGFP-L10a construct (right panel); images acquired at 50,000× magnification, inserts enlarged by a factor of 2.3×.

There was no gross alteration in cell physiology or growth rate was evident in eGFP-L10a transfected cells, as seen by fluorescence microscopy of eGFP-L10a expression in cells. HEK293T cells grown on coverslips were transiently transfected, grown for two days, fixed with paraformaldehyde, and mounted with media containing 4′,6-diamidino-2phenylindo (DAPI) to stain DNA.

Example 2 Isolation, Purification and Analysis of Ribosome-mRNA Complexes In Vitro

Rapid immunoaffinity purification of polysomes was achieved from HEK293T cells transiently transfected with the eGFP-L10a transgene but not mock transfected cells. HEK293T cells transfected with eGFP-L10a were homogenized in lysis buffer. The solubilized and clarified lysate was loaded onto a linear density (20-50% w/w) gradient of sucrose and centrifuged for 2 hours at 4° C. using a Beckman SW41 rotor at 40,000 r. p. m. (200,000×g). 750 μl fractions were collected as absorbance at 254 nm was monitored with an ISCO UA-6 UV detector.

Immunoaffinity purification of polysomes from transfected cell cultures (in which approximately 30% of cells expressed eGFP-L10a) gave an approximate 10% overall co-purification of untagged ribosomal proteins and ribosomal RNA, and led to the recovery of only translated mRNAs (Table 3).

TABLE 3 Total RNA yields from cultured cell BACarray purifications. HEK293T cells were transiently transfected with eGFP (mock transfection) or eGFP-L10a constructs. Transfection efficiency was approximately 30%. Plates of different sizes were used to grow mock or eGFP-L10a-transfected cells, which is reflected in different input RNA amounts. Input and immunoprecipitated (bound) RNA were purified and the quantity and purity of RNA were determined using a Bioanalyzer 2100 (Agilent Technologies). Total RNA Total RNA Yield Transfection Bound (ng) Input (ng) (%) eGFP (Mock) 61 33,320 0.2 eGFP-L10a 6,117 56,630 10.8

FIG. 3 displays immunoprecipitation of translated mRNAs from transfected cells. HEK293T cells were transiently transfected (˜30% efficiency) with eGFP (mock) or eGFP-L10a constructs and grown in medium alone (no treatment, NT) or in medium supplemented with 100 μg/ml ferric ammonium citrate [pH 7.0] (iron) for 36 hours. (a) Induction of the iron-storage protein Ferritin was seen after 36 hours of iron treatment. (b) The post-mitochondrial supernatants of untreated or iron-treated cells were loaded onto linear sucrose gradients (20-50% w/w). After velocity sedimentation, fractions (direction of sedimentation noted by arrow) were collected while UV absorbance (254 nm) was being measured. A representative trace is shown, as profiles from non-treated and iron-treated lysates looked nearly identical. Non-polysome and polysome gradient fractions were generated as indicated. To avoid Ferritin mRNAs associated with mRNPs, only heavier polysomes (with greater than 4 ribosomes) were included in the polysome fraction. (c) After iron treatment, as expected, a shift of Ferritin heavy chain mRNA (Fth1) out of non-polysome fractions into polysome fractions was observed, as determined by reverse transcription followed by quantitative PCR of total RNA purified from non-polysome and polysome gradient fractions (range of fold-change in non-polysome fraction: 0.16-0.22; range of fold-change in polysome fraction: 1.83-2.15). (d) Immunoprecipitation were performed from non-polysome or polysome gradient fractions and 0.2% of the Input (IN), 0.02% of the unbound (UB), and 1% of the bound (IP) samples were loaded onto gels for immunoblot analysis with eGFP or Rpl7 antibodies; * indicates the presence of a weak Rpl7 band upon much longer exposure. eGFP-L10a was recovered equally well from non-polysome and polysome fractions with or without iron treatment. Rpl7 was recovered equally well from untreated or iron-treated polysome fractions. Rpl7 was not present in the non-polysome fraction, presumably because, unlike the overexpressed eGFP-Rpl10a, it was all incorporated into polysomes. As expected from the lack of Rpl7 (and thus assembled ribosomes) in the non-polysome fraction, immunoprecipitation from non-polysome fractions did not pull down any RNA above background, indicating that the immunoprecipitation was specific to translated messages. (e) To determine if the translating ribosome affinity purification (TRAP) methodology could faithfully reflect the changes observed in c, a direct eGFP immunoprecipitation of post-mitochondrial supernatants (whole cell lysates, unfractionated) was performed. 0.5% of input (IN), 0.5% of the unbound fraction (UB), and 1.0% of the bound (IP) samples were loaded onto gels for immunoblot analysis with eGFP (top), Rpl10a (middle), or Rpl7 (bottom) antibodies. eGFP-L10a was recovered equally well from untreated and iron-treated samples, as was all eGFP from mock samples; * indicates the presence of a light eGFP-L10a band upon longer exposure. No endogenous Rpl10a or Rpl7 was recovered in the bound (IP) fraction of mock samples, while endogenous Rpl10a and Rpl7 were both recovered in the bound (IP) fraction of untreated and iron-treated samples. The reduced recovery of endogenous Rpl10a versus endogenous Rpl7 in the immunoprecipitation likely reflects competition between endogenous Rpl10a and eGFP-L10a for incorporation into ribosomes. (f) The iron-induced fold-change in Fth1 mRNA levels relative to Actb mRNA levels in samples immunoprecipitated (IP) from either polysomes or from whole cell lysates (range of fold-change in polysome IP samples: 2.01-2.43; range of fold-change in direct IP samples: 1.80-1.93) was similar to the change observed in the polysome gradient fraction before immunoprecipitation (c).

Methods

Standard methods for immunoblotting were used. Antibodies were used as follows: GFP detection: JL-8, Clontech (Mountain View, Calif.), 1:2,000 in 5% non-fat milk/PBST (phosphate buffered saline-0.05% Tween-20]; Rpl7 detection: NB200-308, Novus Biologicals (Littleton, Colo.), 1:2,000 in 5% IgG-free bovine serum albumin/PBS-T; Rpl10a detection: H00004736-M01, Abnova Corporation (Taipei City, Taiwan), 1:2,000 in 5% IgG-free bovine serum albumin/PBS-T; Ferritin detection: 65077, MP Biomedicals (Solon, Ohio), 1:1,500 in 0.2% I-block (Applied Biosystems, Foster City, Calif.)/PBS-T; β-Actin detection: Ab8224, Abcam (Cambridge, Mass.), 1:2,500 in 5% nonfat milk/PBS-T.

Example 3 Characterization and Analysis of BACarray Transgenic Mice

1) Overview

Comparative analysis of BACarray data can provide important mechanistic insights into complex biological systems. BACarray translational profiling permits comprehensive studies of translated mRNAs in genetically defined cell populations, and their responses to physiological perturbations. To establish the generality of this approach, BACarray translational profiles for twenty four distinct and diverse CNS cell populations are presented here. Identification of cell-specific and enriched transcripts, previously not identified in whole tissue microarray studies are provided as examples to illustrate the added value of comparative analysis of these large datasets. The BAC transgenic strategy has been applied (Heintz, 2004; Yang et al., 1997) to provide high resolution anatomical data and BAC vectors for the design of genetic studies of specific, morphologically defined cells in the CNS (Gong et al., 2003) (world wide web address: gensat.org). Heiman et al. have reported the development of the BACarray translational profiling methodology for use in discovery of the complement of proteins synthesized in any genetically defined cell population. Here, the methodology is used to generate BACarray transgenic mice for a wide variety of anatomically and genetically defined cell types in the mammalian brain. In some embodiments, such a collection can provide a resource that will allow detailed molecular phenotyping of CNS cell types at specified developmental stages, and in response to a wide variety of pharmacological, genetic or behavioral alterations. The mice and data presented confirm the generality of the BACarray approach and provide a resource for studies of the molecular bases for cellular diversity in the mammalian brain.

The generation of BACarray translational profiles of specific CNS cell types requires targeting of the eGFP-L10a ribosomal protein fusion to desired CNS cell types, affinity purification of polysomal RNAs from these cell types, and interrogation of the resultant mRNA populations using massively parallel analytical techniques. To provide a resource for comparative analysis of diverse CNS cell types, described in this examples is: the application of this strategy to generate and characterize further BACarray transgenic lines, to isolate and characterize mRNA populations from twenty four cell types targeted in these lines, to analyze these data relative to one another, and to archive and present the BACarray transgenic lines and their associated anatomic and microarray data.

2) Selection of Drivers to Target Specific CNS Cell Types

To confirm the general applicability of the translating ribosome affinity purification (TRAP) methodology, and to obtain initial data concerning the depth of information one can obtain with this approach across multiple cell types, BACs reported by the Gene Expression Nervous System Atlas (GENSAT) Project (Gong et al, 2003; S. Falcon, R. Gentleman, Bioinformatics 23, 257-8 (2007); both incorporated in their entirety) were chosen to specifically target a wide range of neurons and glia from different structures throughout the CNS.

3) BACarray Mice

To genetically target expression of the eGFP-L10a fusion protein to defined CNS cell populations in vivo, BAC transgenic mice were created (FIG. 4 represents the BACarray strategy; FIGS. 5 and 6 represent engineering of BAC vectors and the creation of mice carrying BACs). To tag mRNAs in specific cell types of the mouse, cell specific cis and trans regulatory elements and known transcriptional units were utilized as described herein and in (Gong et al. Nature Vol 425, 2003). Mouse lines with specific expression in different subsets of cells in the cerebral cortex (FIG. 7) or hypothalamus (FIG. 8) from BAC array lines are presented by way of example. Detailed anatomic characterization of selected BACarray transgenic mouse lines is presented below.

4) Anatomic Characterization of BACarray Transgenic Mouse Lines

Detailed anatomic studies were conducted for BACarray transgenic mouse lines as displayed in FIG. 9. For each line, transgene expression was assayed by immunohistochemistry (IHC) using an antibody against eGFP. IHC data from serial coronal sections of the whole brain were collected, scanned at high resolution and mounted for inspection. All sections were processed equivalently (Neuroscience Associates) so that apparent differences in staining intensity accurately reflect differences in transgene expression level. The figure shows thumbnail images for each of the 24 cell populations selected for microarray analysis, and high resolution data to illustrate the morphology evident from IHC analysis of eGFPL10a expression. The regions covered in this characterization include the cerebellum (panels 1-9), the spinal cord (10), the basal forebrain and corpus striatum (11-14), the brainstem (15), and cerebral cortex (16-25).

For well characterized cell types, anatomic confirmation of the cells targeted for BACarray analysis was facilitated. For example, given the well described cytoarchitecture of the cerebellum, BACarray lines for Purkinje cells (Pcp2, panel 1), granule cells (Neurod1, panel 2), Golgi neurons (Grm2, panel 3), unipolar brush cells (Grp, panel 5), Bergmann glia (Sept4, panel 8), and astrocytes (Aldh111, panel 9) were easily identified from the IHC data presented. Purkinje cells can be recognized in the eGFP-L10a IHC data by their large cell bodies and molecular layer dendrites, granule cells by their small size, dense packing, and location in the granule layer, and Bergmann glial cells by their morphology, radial projections, and close proximity to Purkinje cells. IHC analysis of eGFP-L10a fusion proteins in these well known cell types using these regulatory regions allow for identification of well known and previously unknown cell types. The expression of the eGFP-L10a transgene from each BAC driver is regionally correct, conforming both to the literature and to the expectation given the anatomic data presented in GENSTAT and for these cell types the distribution of the eGFPL10a fusion protein, while more limited than that of soluble eGFP, is sufficient to provide enough anatomic detail to unambiguously identify these well described CNS cell types.

Many BACarray lines, the eGFP-L10a fusion protein is detected in multiple structures within the brain. An example is the characterization of fusion protein IHC in cholinergic cell populations targeted in the Chat BACarray lines. In this case expression is clear in brainstem motor neurons, spinal cord motor neurons, neurons of the corpus striatum, basal forebrain projection neurons, and neurons of the medial habenula. As detailed below, BACarray translational profiles for four of these cholinergic cell populations were collected by separately dissecting the spinal cord, brainstem, corpus striatum and basal forebrain prior to affinity purification of the eGFPL10a tagged polysome populations. Since specifically expressed genes are often found in distinct cell types in physically separable brain structures, several lines offer opportunities for analysis of many cell types not included in this example. Thus, the eGFP-L10a fusion protein is abundant in hippocampal CA1 cells in the Cck BACarray line, allowing translational profiling of mRNAs expressed in this cell type (panel 25). Creation of a BACarray anatomic database allows the user to browse through serial brain sections for each of the lines presented here to determine whether a cell type of interest can be analyzed in one of the BACarray lines presented in this example.

The figure also shows that projection neurons in the cerebrum can be identified by their pyramidal shape, and broadly classified by their laminar specificity, dendritic arbor, and axonal targets. Lines which clearly label the large pyramidal cells of layers 6 (Ntsr1, panel 16), 5b (Glt25d2, panel 17), and 5a (Etv1, panel 18) have also been reproduced. Morphometric studies provide additional data indicating that the GENSAT eGFP lines and eGFP-L10a BACarray lines target similar cortical pyramidal cell populations FIG. 10 shows that BACarray cortical pyramidal cells have the same laminar distribution as eGFP GENSTAT lines. The figure shows: A) Graph (mean+/−SE) of the distance of eGFP+ cell somas from the pial surface for the BACarray and GENSAT eGFP lines for each pyramidal cell BAC driver. The depth of the cells was consistent between both lines for each driver. B) The percentage of cells in 100 micron bins is shown as a histogram of the distribution of cell depths for each line in A. The BACarray line and eGFP line for each driver had overlapping distributions of cell depths.

FIG. 11 shows that in many BACarray lines, the presumed cellular identity of the targeted lines was confirmed using double immunofluorescence for the eGFP-L10a fusion protein and well characterized cell type specific markers. In some cases, the cell type specific markers corresponded with the BAC drivers chosen for modification (Olig2, Aldh1l1, Grm2, Chat). In other cases, commonly used markers which have been well characterized for specific cell types were used, as seen in panel B. In most cases, these studies established that BAC drivers limited expression to well-defined cell populations. There were also several BACarray lines in which the transgene is expressed in two or more cell types. For example, the immunofluorescence (IF) analysis of the Lypd6 BACarray line revealed that eGFP-L10a is found in all Pvalb positive and NeuN negative interneurons of the cerebellar molecular layer, suggesting that this line is valuable for analyses of both stellate and basket cells. Finally, in certain lines it is apparent that the eGFP-L10a transgene is expressed in a only a subset of a particular cell type. For instance, as seen in panel B, in the Grp BACarray line the eGFP-L10a fusion protein is restricted to the subpopulation of unipolar brush cells (Nunzi et al., 2002) which are immunoreactive for Grm1 but not Calb2 (calretinin).

BACarray lines whose expression did not conform to readily identified cell types, were also analyzed by IF analysis to provide data concerning the broad classification of cell populations targeted. For example, in the cerebral cortex of the Cort BACarray line, Calb1 was detected in nearly 50% of eGFP-L10a positive cells, Pvalb was found in less than 5% of these cells, and Calb2 was not detected. Characterization of the fusion protein in the cortex of Pnoc BACarray mice revealed that the majority of eGFP-L10a positive cells in the superficial layers of the cerebral cortex are multipolar and are GABA positive, although some cells in deeper layers of cortex are GABA negative and appear to have a single apical dendrite. The multipolar cells in this case are often positive for Calb2, but not Calb1 or Pvalb. Both IHC and IF studies of the cortex of the Cck BACarray line clearly demonstrate that eGFP-L10a is detected in small neurons positive for Calb1 but not Pvalb or Calb2, as well as in pyramidal cells (data not shown), consistent with previous ISH data (world wide web address: stjudebgem.org; world wide web address: brain-map.org) (Lein et al., 2007; Magdaleno et al., 2006).

In addition to neuronal cell types, three BACarray lines for glial cell types were generated which were analyzed in both cerebellar and cortical tissue. These glial cell types included astrocytes, mature oligodendrocytes, and a mixed oligodendroglial line that included mature oligodendrocytes and oligodendrocyte progenitors (also called synantocytes or polydendrocytes) (Butt et al., 2005). Astrocytes were targeted using a BAC for the gene Aldh1l1 that has previously been described as astrocyte specific (Anthony and Heintz, 2007; Cahoy et al., 2008). This BAC drove transgene expression in both Gfap+ (reactive) and Gfap− astrocytes, as well as Bergmann glia. It did not express in Ng2+ oligodendrocyte progenitors, nor in Cnp+ myelinating oligodendrocytes. In contrast, a BAC for the Olig2 transcription factor directed expression specifically into both the Ng2+ and Cnp+ oligodendrocyte lineage cells. Finally, a BACarray line for Cmtm5 expressed specifically, albeit weakly, in mature (Cnp+) oligodendrocytes. With these three lines, the translational profile of the three major classes of glia across the CNS can be examined.

In cases of relatively weakly expressing lines, such as Cmtm5, new drivers can be selected to more effectively target the same cell type. Studies with a mature oligodendrocyte line (Cnp JD368) have demonstrated improved RNA yield and data quality from a more strongly expressing transgene (FIG. 12).

5) Methods

BAC Modification, Transgenesis, and Animal Husbandry: BACs from

Table 4 were modified as described to insert an eGFP-L10a fusion protein into the translation start site of the driver gene (Gong et al., 2002; Gong et al., 2003). Founders and subsequent generations were bred to either Swiss-Webster or c57b1/6 wildtype mice. Lines were maintained as trans-heterozygotes.

TABLE 4 List of BACs utilized for transgenesis Included are the list of abbreviations used throughout for each gene, and the ID for the BAC clone that was modified. Abbreviation Full Gene Name BAC Sept4 Septin 4 RP23-21 N23 Aldh111 Aldehyde Dehydrogenase 1 family, RP23-7N19 member L1 Cmbm5 CKLF-like MARVEL transmembrane RP24-317F19 domain containing 5 C ck Cholecystokinin RP23-234l17 C hat Choline Acetyltransferase RP23-431D9 C ort Cortistatin RP23-281A14 Drd2 Dopamine receptor 2 RP23-161H15 Drd1 Dopamine receptor D1A RP23-47M2 Grp Gastrin-Releasing Peptide RP23-179M10 Grm 2 Glutamate Receptor, Metabotropic 2 RP23-335E12 Glt25d2 Glycosyltransferase 25 Domain-containing RP23-160M1 2 Lypd6 LY6/PLAUR domain containing 6 RP23-14O24 NeuroD1 Neurogenic Differentiation 1 RP24-151C22 Ntsr1 Neurotensin Receptor 1 RP23-314D14 Olig2 Oligodendrocyte Transcription Factor 2 RP23-356P18 Pnoc Prepronociceptin RP23-264L8 Pcp2 Purkinje Cell Protein-2 RP24-186D18 Etv1 Ets1 Variant Gene 1 RP23-250K4

Immunohistochemistry: Six to twelve week old mice were euthanized with CO2 and perfused transcardially with phosphate buffered saline (PBS) pH 7.4 followed by 4% paraformaldehyde in PBS. For diaminobenzidine tetrahydrochloride (DAB) immunohistochemistry, fixed brains were treated overnight with 20% glycerol and 2% dimethylsulfoxide to prevent freeze-artifacts. Multiple brains (up to 25 per block) were embedded in a gelatin matrix using MultiBrain™ Technology (NeuroScience Associates, Knoxville, Tenn.). After curing, the block was rapidly frozen to −70° C. by immersion in a mixture of isopentane and crushed dry ice, and mounted on a freezing stage of an AO 860 sliding microtome. The MultiBrain™ block was sectioned coronally at 40 microns. All sections were collected sequentially into the wells of a 4×6 plate filled with Antigen Preserve solution (50% PBS pH 7.0, 50% Ethylene glycol, 1% Polyvinyl Pyrrolidone).

After blocking with hydrogen peroxide and serum, the sections were incubated with a 1:75,000 solution of Goat anti-eGFP serum (Heiman et al) overnight at room temperature. Following extensive washing, the sections were incubated with biotinylated secondary antibody (Anti Goat IgG, Vector Labs, Burlingame, Calif.), washed again, and incubated with avidin-biotin-HRP (Vectastain elite ABC kit, Vector Labs, Burlingame, Calif.) according to the manufacturer's instructions. Sections were again washed and incubated with DAB and hydrogen peroxide until fully developed. Finally, developed sections were mounted on gelatinized (subbed) glass slides, air dried, dehydrated in alcohols, cleared in xylene and coverslipped. Images were acquired with a Zeiss Axioskop2 microscope with a 10× (1.5 NA) objective using an automated x,y stage (Marzhauser scan8) controlled by a PC with Zeiss KS400 software running custom macros. Wildtype brains showed no labeling.

For fluorescence immunohistochemistry, fixed brains were cryoprotected in 30% sucrose in phosphate buffered saline (PBS), frozen, cut to 40 micron serial floating sections by cryostat, and stored in PBS 0.1% Sodium Azide at 4° C. until use. Sections were blocked in PBS with 5% normal donkey serum, 0.25% triton for 30 minutes, then incubated overnight with primary antibodies (Table 5). Sections were washed in PBS, exposed to appropriate Alexa dye conjugated secondary antibodies (Molecular Probes/Invitrogen, Carlsbad, Calif.) for 90 minutes, washed, then mounted. All images were acquired with a Zeiss Inverted LSM 510 laser scanning confocal microscope, with a Z thickness of 2 microns. Z-stacks through the sections were acquired to confirm colocalization.

TABLE 5 Antibodies, product numbers, and sources used for immunohistochemistry. Symbol Antibody Name Product Number Source EGFP Goat Anti-EGFP NA (Heiman et al.) EGFP Chicken Anti-EGFP AB19370 Abeam, Cambridge Ma. NEUN Neuronal Nuclei MAB377 Chemicon, Temecula, Ca. OLIG2 Oligodendrocyte transcription factor 2 AB9610 Chemicon, Temecula, Ca. CSPG4 Ng2 Proteoglycan AB53420 Chemicon, Temecula, Ca. CHAT Choline Acetyl-Transferase AB143 Chemicon, Temecula, Ca. GRM2/GRM3 metabotropic glutamate receptor 2&3 AB1553 Chemicon, Temecula, Ca. GRM1 metabotropic glutamate receptor 1 AB1551 Chemicon, Temecula, Ca. CNP1 Cnpase MAB326R Chemicon, Temecula, Ca. CALB1 Calbindin 300 Swant, Bellinzona, CH CALB2 Calretinin 6B3 Swant, Bellinzona, CH PVALB Parvalbumin Pv28 Swant, Bellinzona, CH GFAP Glial Fibrillary acidic protein Z0334 Dako, Denmark S100 S100 Z0311 Dako, Denmark GLUL Glutamine Synthetase G2781 Sigma-Aldrich, St Louis, Mo. SLC18a3 Vesicular Acetylcholine Transporter sc-7717 Santa Cruz Biotech, Santa Cruz, Ca. ALDH1L1 10-Formyltetrahydrofolate NA a gift from Dr. Robert Cook Dehydrogenase — GABA A2052 Sigma-Aldrich, St Louis, Mo.

Fixed Sections: For fixed sections, BACarray transgenic mice were deeply anesthetized with pentobarbitol or 50 mg/ml Nembutal and transcardially perfused with 10 ml of phosphate buffered saline (PBS) followed by 40 ml of 4% paraformaldehyde (PFA) in PBS. Brains were dissected and post-fixed at room temperature for exactly 1 h with 4% PFA in PBS. After fixation, brains were washed 3 times in PBS and incubated in 5% weight/volume (w/v) sucrose in PBS at 4° C. for 1 hour with gentle agitation. Brains were then incubated for 24 h in 15% w/v sucrose in PBS at 4° C. with gentle agitation and for 24 h in 30% w/v sucrose in PBS at 4° C. with gentle agitation. Brains were placed in an embedding mold filled with Neg-50 embedding medium (Richard Allan Scientific, Kalamazoo, Mich.) for 1 hour at room temperature, and were subsequently incubated on dry ice for 1 hour to freeze the embedding medium. Brains were then transferred to and stored at −80° C. until sectioned. 12 μm sagittal sections were cut, mounted on glass slides, and kept overnight at −20° C., then transferred to −80° C. until used for immunohistochemistry.

Before use sections were thawed and dried at room temperature for 20 min, washed with PBS, and incubated in 0.2% H2O2/PBS at room temperature for 30 min to quench endogenous peroxidase activity. Sections were washed with PBS, permeabilized with PBS/0.05% Tween-20, and blocked with Image-it FX signal enhancer (Invitrogen Corporation, Carlsbad, Calif.) for 30 min at room temperature. Sections were washed with PBS/0.05% Tween-20 and blocked again with 2% donkey serum/0.1% fish gelatin/PBS/0.05% Tween-20. Sections were then incubated overnight at 4° C. with primary antibodies. The next day, sections were washed with PBS/0.05% Tween-20 and incubated with anti-rabbit Superpicture HRP Polymer Detection Kit (Invitrogen Corporation, Carlsbad, Calif.) for 1 h at room temperature. Sections were washed with PBS/0.05% Tween-20 and a fluorescent HRP substrate, Tyramide-AlexaFluor 546 conjugate (from TSA kit #13, Invitrogen Corporation, Carlsbad, Calif.), was deposited on the slides. Slides were washed again with PBS/0.05% Tween-20, mounted with Prolong Gold Antifade (Invitrogen Corporation, Carlsbad, Calif.), dried overnight, and fluorescence was visualized on a Zeiss LSM510 confocal microscope (Carl Zeiss, Thornwood, N.Y.).

Fresh Frozen Sections: For all nuclear immunohistochemistry (Anti-Atrx, Anti-PML, Anti-γH2A.X), fresh frozen brain sections were used. For fresh frozen brain sections, BACarray mice were euthanized with CO2, decapitated, and their brains removed into ice-cold PBS. Brains were then embedded in Neg50, placed on dry ice for an hour, and stored until needed at −80° C. Before sectioning, frozen blocks of tissue were equilibrated at −20° C., and 16 um sections were cut on a Leica cryostat. Slides with fresh frozen brain sections were stored at −80° C. or −20° C. until further use.

Prior to use, sections were allowed to air-dry at room temperature for 15 minutes, then fixed with 1% freshly made PFA in 1×PBS for 10 minutes at room temperature. Next, slides were washed three times with 1×PBS, and permeabilized for 10 minutes at room temperature with 0.05% Triton X-100 in PBS. After permeabilization, tissue sections were blocked using either 5% horse serum or 5% goat serum in PBS for at least one hour. Sections were incubated with primary antibodies overnight in a humidified chamber at 4° C., washed three times with PBS, and incubated at room temperature for one hour with appropriate secondary antibodies. After incubation with secondary antibodies, sections were incubated for 10 minutes with the nuclear stain TO-PRO-3 (Invitrogen Corp., Carlsbad, Calif.), washed three times with PBS, and coverslipped with Aquamount mounting medium (Lerner Laboratories, Pittsburgh, Pa.). Images were collected on an upright Zeiss Axioplan LSM 510 laser scanning confocal microscope (Carl Zeiss MicroImaging, Inc., Thornwood, N.Y.) using 40×/1.2, water immersion objective, 63×/1.4 oil immersion, and 100×/1.4 oil immersion lenses.

Quantification of laminar position of cortical pyramidal cells: Anti-eGFP immunohistochemistry with DAB was performed on 20 micron sagittal sections from the Etv1 TS88, Glt25d2 DU9, and Ntsr1 TS16 BACarray lines as described (Gong et al), and images were acquired as above. Corresponding digital images of the adult sagittal sections were downloaded from gensat.org for lines expressing eGFP from the same BACs. Sections containing motor cortex (corresponding to Paxinos section 111) (Paxinos and Franklin, 2001) were imported into ImageJ (rsb.info.nih.gov/ij). The distance from the apical tip of the soma to the pial surface was measured using the ‘straight line selection’ tool. The apical tip was defined as the site at which the apical dendrite and the cell body converge. Only cells with a clearly visible apical dendrite and a uniformly stained soma were measured. At least 50 cells were measured from each image. All measurements were then converted from pixels to microns using the following scale: 1 pixel=1.33 microns.

In situ hybridization: IMAGE consortium clones containing sequences from genes of interest were purchased from Open Biosystems (Table 6). Probes were synthesized by linearization of plasmid with appropriate restriction enzyme, template purification with Qiagen PCR purification kit, followed by in vitro transcription with appropriate enzyme (T3 or T7) using DIG RNA labeling kit (Roche, Basel, CH). Labeled RNA was purified with ProbeQuant G-50 microcolumns (GE Healthcare), and assayed for quality and quantity with a Bioanalyzer, following manufacturer's instructions (Agilent Technologies, Santa Clara Calif.).

TABLE 6 List of plasmids of in situ hybridization studies: Genbank Open Biosystems # Gene Name Enzymes PNOC Gene ID B230118H07Rik BC025075 MMM1013-7513748 RIKEN cDNA B230118H07 gene Sal I cut, T7 polymerase Chd4 BC058578 MMM1013-9201124 chromodomain helicase DNA Sal I cut, T7 polymerase binding protein 4 Cidea AA061879 EMM1002-1115733 cell death-inducing DNA Eco RI cut, T3 polymerase fragmentation factor, alpha subunit-like effector A Crabp1 BC065787 MMM1013-9202137 cellular retinoic acid binding Eco RI cut, T3 polymerase protein I Ddef1 BC094581 MMM1013-98479313 development and differentiation Eco RI cut, T3 polymerase enhancing Igf1 BC012409 MMM1013-65370 insulin-like growth factor 1 Sal I cut, T7 polymerase GRM2 Gene ID Lypd1 CA328245 EMM1002-6960067 RIKEN cDNA 2700050C12 gene Eco RI cut, T3 polymerase Slc6a5, GlyT2 BM941867 EMM1032-584726 solute carrier family 6 Eco RI cut, T3 polymerase (neurotransmitter transporter, glycine), member 5 Amn AA023455 EMM1002-1077429 amnionless Eco RI cut, T3 polymerase Penk1 AA098193 EMM1002-1183311 preproenkephalin 1 Eco RI cut, T7 polymerase Gcqr BC031885 MMM1013-7511930 glucagon receptor Sal I cut, T7 polymerase Ceacam 10 BC003346 MMM1013-62963 CEA-related cell adhesion Sal I cut, T7 polymerase molecule 10 ISH was conducted on 11 genes enriched in either Grm2 positive granule cell layer interneurons, or Pnoc positive neurons of cortex, using plasmids ordered from Open Biosystems. Plasmids were sequenced to confirm the accuracy and orientation of the ESTs. Plasmids were linearized with either Sal I or Eco RI and Dig labeled RNA was transcribed with either T3 or T7 polymerase to create anti-sense cRNA probe.

Adult mice were processed as above, and brains were cut on cryostat into 20 micron sections and mounted onto Fisher Superfrost Plus slides. Tissue was washed, then post fixed for 20 minutes in 4% paraformaldehyde PBS, permeabilized with 0.05% Triton X100, digested with 50 ug/ml Proteinase K in TE for 10 minutes, and acetylated for 10 minutes in 0.1M TEA with 0.25% acetic anhydride. Sections were prehybridized in Atlas hybridization chambers (Clontech, Mountain View, Calif.) in 5×SSC, 2.5×Denhardt's solution, with 500 ug/ml sheared denatured salmon sperm for 30 minutes, then hybridized overnight with labeled riboprobe. Slides were washed for 5 minutes at 65° C. with 5×SSC, then for 1 hour with 0.2×SSC at 68° C., and for 5 minutes at room temperature. Slides were washed in TBS (100 mM Tris HCl, 150 nM NaCl, pH 7.5) twice for five minutes, blocked with 10% Roche blocking solution in TBS for one hour, incubated with primary antibodies for 1 hour in the same solution (Sheep anti-Dig, alkaline phosphatase conjugated, Roche 11 093 274 910) and Goat anti-eGFP where appropriate, washed with TBS, and developed with NBT/BCIP or HNPP/Fast Red following manufacturers protocols (Roche). For eGFP/ISH double fluorescence, sections were then incubated with alexa-488 donkey anti-goat antibody (Invitrogen) for one hour before counterstaining with DAPI, washing and coverslipping. Images were acquired with Zeiss Inverted LSM 510 laser scanning confocal microscope.

Example 4 Isolation and Purification of Ribosome-mRNA Complexes In Vivo

Improved procedures for rapid extraction and immunoaffinity purification of the eGFP-tagged functional polyribosome complexes from in vivo intact brain tissue were developed and optimized (A. Alexa, J. Rahnenfuhrer, T. Lengauer, Bioinformatics 22, 1600-7 (2006)). Highly purified RNA and protein was consistently obtained from BACarray mice. Key steps of the purification protocol included rapid manual dissection and homogenization of the tissue in question, inclusion of magnesium and cycloheximide in the lysis buffer to maintain ribosomal subunits on mRNA during purification, inhibition of endogenous RNase activity, solubilization of rough endoplasmic reticulum-bound polysomes under nondenaturing conditions, use of high-affinity anti-eGFP antibodies, and the addition of high-salt washes after immunoaffinity purification to reduce background. FIG. 13 displays purification of eGFP-tagged L10a and co-purification of untagged ribosomal protein L7 from D1 BACarray animals but not wild-type littermates in (a) (D1, samples from D1 BACarray mice; WT, samples from wild-type littermates; In, 1% Input; UB, 1% Unbound; IP, 6.5% Immunoaffinity purified sample). eGFP-L10a signal is only present in the D1 IP lane because the IP samples were more concentrated relative to In and UB. In (b) purification of 18 S and 28 S rRNA from D1 BACarray transgenic animals (top panel) but not wild type littermates (bottom panel) as detected by Bioanalyzer PicoChips (Agilent Technologies) is shown. 28 S rRNA runs at ˜47 sec, 18 S rRNA runs at ˜43 sec, and the marker peak runs at ˜23 sec.

Following immunoprecipitation of ribosomal complexes, electron microscopy was utilized to visualize the ribosomes. Aliquots of anti-GFP coated magnetic beads were fixed in 2.5% glutaraldehyde/0.1M cacodylate [pH 7.4] on ice. The bead pellet was post-fixed with 1% osmium tetroxide in the same buffer on ice. After treatment with 0.5% aqueous uranyl acetate at room temperature, the specimen was dehydrated with graded alcohol (70, 90, 100%) and treated with propylene oxide before embedding in Embed 812 resin. The resin was polymerized in a 60° C. oven for 2-3 days. Silver sections were cut with a Dupont diamond knife on a Reichert-Jung UltraCut E ultramicrotome. The sections were collected on copper grids, doubly stained with saturated, aqueous uranyl acetate and lead citrate before examination with a Jeol 100cx electron microscope (JEOL, Peabody, Mass.) operated at 80 kV (FIG. 14).

1) Generation of Monoclonal Antibodies

All immunoprecipitation except for the Drd1 and Drd2 lines, which used the Goat Anti-eGFP described in later examples, were done using two monoclonal anti-eGFP antibodies (clones 19C8 and 19F7) specifically generated for this purpose at the Monoclonal Antibody Core Facility at Memorial Sloan-Kettering cancer center. Mice were immunized with purified GST-eGFP fusion protein and several rounds of screening were performed to identify clones which functioned well in immunoprecipitation assays. Initially, monoclonal supernatants were tested by ELISA using 96 well plates coated with eGFP purified from transiently transfected 293T cells. Next, positive clones were screened in immunoprecipitation assays, again using the eGFP purified from transfected 293T cells. Finally, positive clones which strongly immunoprecipitated eGFP from lysates from a transgenic mouse line expressing eGFP under the BAC driver of interest were identified.

2) Immunoprecipitation of Polyribosomes

Immunoprecipitation of polyribosomes and isolation of mRNA was done as described in detail below, except goat anti-eGFP antibody was substituted with a mix of two monoclonal eGFP antibodies (19C8, 19F7). Three to six mice for each replicated sample were euthanized with CO2 and distinct brain regions (cerebellum, cortex, corpus striatum, basal forebrain, brainstem, or spinal cord) were dissected Each cell population was assayed in triplicate. RNA quality control, amplification and hybridization were done as described by Heiman et al. For consistency across cell types, 15 ngs of total RNA were amplified for each sample.

Example 5 Analysis of mRNA from BACarray Mice 1) Preparation of mRNA

For mRNA purification, mice were decapitated and the specific tissue of interest was quickly dissected from mice brains. Pooled tissue was immediately collected in ice-cold dissection buffer and homogenized in ice-cold polysome extraction buffer (10 mM HEPES [pH 7.4], 150 mM KCl, 5 mM MgCl2, 0.5 mM dithiothreitol, 100 μg/ml cycloheximide, protease inhibitors, and recombinant RNase inhibitors) using a motor-driven Teflon-glass homogenizer. Homogenates were centrifuged for 10 minutes at 2,000×g, 4° C., to pellet large cell debris, and NP-40 or IGEPAL (EMD Biosciences, San Diego, Calif.; Sigma, St. Louis, Mo.) and DHPC (Avanti Polar Lipids, Alabaster, Ala.) were added to the supernatant at a final concentration of 1% and 30 mM, respectively. After incubation on ice for 5 minutes, the clarified lysate is centrifuged for 10 minutes at 13,000×g to pellet unsolubilized material. Goat anti-GFP (custom made) coated protein G Dynal magnetic beads (Invitrogen Corporation, Carlsbad, Calif.) are added to the supernatant and the mixture was incubated at 4° C. with end-over-end rotation for 30 minutes. Beads were subsequently collected on a magnetic rack, washed three times with high-salt polysome wash buffer (10 mM HEPES [pH 7.4], 350 mM KCl, 5 mM MgCl2, 1% NP-40, 0.5 mM dithiothreitol, 100 μg/ml cycloheximide) and immediately placed in TriZol-LS reagent (Invitrogen Corporation, Carlsbad Calif.) and chloroform to extract the bound rRNA and mRNA from polysomes. After extraction, RNA was precipitated with sodium acetate and Glycoblue (Ambion, Austin, Tex.) in isopropanol overnight at −80° C., washed twice with 70% ethanol, resuspended in water, and further purified using an Rneasy Micro Kit (Qiagen, Valencia, Calif.) with in-column DNase digestion. Purified samples were analyzed using a Bioanalyzer (Agilent Technologies, Santa Clara, Calif.) in order to assess mRNA quantity and quality, as reflected by rRNA levels and integrity.

After purification of RNA samples, 1.5 ul was used for quantification using a Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, Del.). Each sample was further analyzed with a Bioanalyzer (Agilent Technologies, Santa Clara, Calif.) to ensure that the quality of each RNA sample met standard criteria and that they were comparable to each other. Specifically, all RNA samples had a 260/280 ratio (Nanodrop) of at least 1.8, and a RNA Integrity Number (Bioanalyzer) of at least 7. Moreover, the quality of the samples was visually estimated using the Bioanalyzer readout to estimate the level of potential degradation or contamination for each sample.

2) Microarray Analysis of mRNA

Once these criteria were met, a total of 15 ng of RNA from each sample was amplified, biotinylated, and fragmented with the Affymetrix two-cycle amplification kit (Affymetrix, Santa Clara, Calif.). After amplification, samples were again quantified with a Nanodrop spectrophotometer, and 20 ug of amplified RNA was fragmented following the Affymetrix protocol. Amplified and fragmented samples were analyzed with a Bioanalyzer before hybridization to Affymetrix mouse 430 2.0 microarrays. All hybridizations were done according to standard Affymetrix protocols at the Rockefeller University Genome Array Center.

Tissue handling and RNA purification for all samples was as described. Purified RNA was converted to double-stranded cDNA using the SuperScript GeneChip Expression 3′Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, Calif.) and the GeneChip T7-Oligo(dT) Primer (Affymetrix, Santa Clara, Calif.). cDNA was used for the in vitro synthesis of cRNA using the MEGAscriptT7 Kit (Ambion, Austin, Tex.). cRNA was purified using the GeneChip Sample Cleanup Module (Affymetrix, Santa Clara, Calif.). 600 ng or less of clean cRNA was used in the second-cycle cDNA synthesis reaction using the SuperScript GeneChip Expression 3′-Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, Calif.) and random primers (Affymetrix, Santa Clara, Calif.). The cDNA was purified using the GeneChip Sample Cleanup Module (Affymetrix, Santa Clara, Calif.). Purified cDNA was used for the in vitro synthesis of biotin-labeled cRNA using the GeneChip IVT Labeling Kit (Affymetrix, Santa Clara, Calif.). cRNA was purified using the GeneChip Sample Cleanup Module (Affymetrix, Santa Clara, Calif.) and fragmented into 35-200 base pair fragments using a magnesium acetate buffer (Affymetrix, Santa Clara, Calif.).

As controls, Affymetrix standard spike-in controls (eukaryotic hybridization kit) were used.

For hybridization procedures and parameters, 10 micrograms of labeled cRNA were hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays (world wide web address: affymetrix.com/products/arrays/specific/mouse430_2.affx) for 16 h at 45° C. The GeneChips were washed and stained according to the manufacturer's recommendations (Affymetrix, Santa Clara, Calif.) using the GeneChips Fluidics Station 450 (Affymetrix, Santa Clara, Calif.). This procedure included staining the chips with phycoerythrin-streptavidin, signal amplification by a second staining with biotinylated anti-streptavidin, and a third staining with phycoerythrin-streptavidin.

For array design, Affymetrix Mouse Genome 430 2.0 arrays were used in all experiments. Information regarding the array design and features can be found at world wide web address: affymetrix.com.

For measurement data and specifications, Mouse Genome 430 2.0 arrays were scanned using the GeneChip Scanner 3000 (Affymetrix, Santa Clara, Calif.). Three biological replicates were performed for each experiment. GeneChip CEL files were subjected to Harshlight analysis to detect if any blemishes were present on the GeneChips (hypertext transfer protocol: asterion.rockefeller.edu/Harshlight/index2.html) (M. Suarez-Farinas, M. Pellegrino, K. M. Wittkowski, M. O. Magnasco, BMC Bioinformatics 6, 294 (2005)). Only GeneChips without major blemishes were used. GeneChip CEL files were imported into Genespring GX 7.3.1 (Agilent Technologies, Santa Clara, Calif.), processed with the GC RMA algorithm, and expression values on each chip were normalized to that chip's 50th percentile. Data were filtered to eliminate genes with intensities in the lower range. Only genes where more than one sample had a normalized intensity larger than 16 (4 in log2 scale) were kept in the analysis. Statistical analysis to determine which genes are differentially expressed in the different conditions was carried out using the Limma package from Bioconductor project (world wide web address: bioconductor.org).

Microarray Normalization and Analysis

MIAME compliant raw data are available from the APNRR server and GEO. Replicate array samples were normalized with quantile normalization (GCRMA). Data were filtered to remove those probesets with low signal (<50) from analysis, as well as those probesets identified as monoclonal background (Table 7), and replicate samples were averaged.

TABLE 7 List of Probesets excluded from analysis Probeset IP/UB WT Symbol Name 1428909_at 374.4 1200015M12Rik novel 1428720_s_at 41.55 2010309G21Rik novel 1453238_s_at 57.87 3930401B19Rik novel 1435640_x_at 159 C8506T novel 1425247_a_at 37.25 Igh-4 immunoglobulin heavy chain 4 iserum lgG′) 1425324_x_at 11.34 Igh-4 immunoglobulin heavy chain 4 iserum lgG′) 1424305_at 14.27 Igj immunoglobulin joining chain 1452417_x_at 68.89 Igk-V28 immunoglobulin kappa chain variable 8 (VE) 1427455_x_at 60.27 Igk-V28 immunoglobulin kappa chain variable 8 (VE) 1427660_x_at 58.46 Igk-V28 immunoglobulin kappa chain variable 8 (VE) 1456162_x_at 861.2 Lmna lamin AC 1455892_x_at 6.874 Lmna lamin AC 1450009_at 12.58 Ltf lactotransferrin 1456456_x_at 122.6 Mela melanoma antigen 1427797_s_at 42.54 Mnda myeloid cell nuclear differentiation antigen 1427798_x_at 313.97 Mnda myeloid cell nuclear differentiation antigen 1452556_at 14.87 Mnda myeloid cell nuclear differentiation antigen 1416957 57.24 Pou2af1 Pou domain, class 2, associating factor 1 1435697_a_at 9.079 Pscdbp pleckstrin homology, Sec7 and coiled-coil domains, binding protein 1436727_x_at 23.84 Sptlc1 serine palmitoyltransferase, long chain base subunit 1 1435137_s_at 53.47 novel 1427932_s_at 26.78 novel To identify mRNAs which interact with monoclonal antibodies or protein G dynabeads in the absence of eGFP, BACarray was performed on a wildtype mouse brain and compared to unbound whole brain RNA. The excluded probesets listed here are those for genes found to be highly enriched (>6 fold) in the wildtype IP/UB as well as enriched in multiple IPs from diverse regions and cell types.

Each IP was then compared to the unbound samples from the same tissue to calculate a ratio of IP/UB as a measure of ‘enrichment’. UB samples generally show little or no depletion of cell-specific RNA following IP, and UB samples from several different IPs from the same tissue were averaged for each comparison. UB samples from corpus striatum (Chat line) and neostriatum (Drd1 and Drd2 lines) were normalized together. IPs were globally normalized to UBs using the Affymetrix biotinylated spike in controls, to correct for any broad biases in scanning and hybridization. For each cell type, Table 9 contains the IP/UB values for all genes with fold change >2 and p<0.05 by Welch's t-Test, with Benjamin and Hochberg FDR multiple testing correction, as calculated by Genespring GX version 7.3 (Agilent).

For three cell populations, a further ‘corrected enrichment’ was calculated. For the Bergmann glial line, Sept4, which also shows low level expression in mature oligodendrocytes, the corrected enrichment is the intersection of the IP/UB analysis described above with a comparison of the Bergman Glial line with the mature oligodendrocyte line, Cmtm5, using the same fold change and statistical criteria applied above. For unipolar brush cell line Grp, which also shows expression in some Bergmann glia, the corrected enrichment is the intersection of the IP/UB enrichment described above with a comparison of Grp with the Bergman glial line, using the same criteria. Finally, a substantial proportion of the RNA in the cerebellum is generated by granule cells, and thus the UB samples are highly enriched in granule cell RNA. Therefore, to identify granule cell genes, the ‘corrected enrichment’ was calculated by comparing granule cell IP to the average of all other cerebellar cell type IPs, using the same criteria as above.

Hierarchical clustering was performed in Genespring using the ‘condition tree’ function with a smoothed correlation metric on the GCRMA normalized data for the 20% of probesets with the highest coefficient of variation.

Shannon entropy was calculated in excel from GCRMA normalized values with the following procedure: After excluding probesets with no signal >100 in at least one sample, normalized expression measurements for each data set were categorized into 5 bins by log base 10 values (1-9, 10-99, 100-999, 1000-9999, 10000-99999), and Shannon entropy was calculated for each gene across 1) just the IP samples, 2) just the UB samples, and 3) across all samples using the following formula (Schneider, 2007).

-   M     H=−ΣPi log 2 Pi     i=1

The 10% of probesets with the highest and lowest entropy across all samples were analyzed using the BINGO plugin for the cytoscape software, using the full mouse Gene Ontologies, a p threshold of 0.01 on hypergeometic tests with Benjamin Hochberg FDR correction for multiple testing (Ashburner et al., 2000; Maere et al., 2005). Results from this analysis are substantially similar to those obtained using the EASE online implementation of Gene Ontologies and EASE statistic (Dennis et al., 2003).

Pearson's correlation with MBP was calculated in Genespring.

Comparative analysis of all cell types, and heat maps were generated with the R statistical software. Data were normalized as above. Then, for each cell type the total probeset list was filtered to remove those probesets with signal less than 50, or IP/UB values less than the average plus two standard deviations of the IP/UB values of the relevant negative control genes, or 1, whichever was lesser. Then, with this filtered list of genes, a fold change was calculated for this cell type versus all other samples, iteratively. For each comparison, the fold changes were ranked from highest to lowest, and these ranks were averaged across comparisons for a cell type. The top one hundred probesets from this average ranking were selected for further analysis.

To assess if the ribosomal immunoprecipitation is biased towards longer transcripts, the signal intensity versus transcript length for all probesets was plotted. No positive correlation between signal intensity and length was detected for any sample (FIG. 15).

Example 6 BACarray Polysome Purification, RNA Extraction and Control Microarray Experiments

1) Overview

In total, twenty four cell populations in five regions were chosen to assay with immunoprecipitation (IP) and genome wide translational profile via microarray, using the translating ribosome affinity purification (TRAP) methodology of Heiman et al (2008). For each cell type, pooled microdissected tissue from three to six transgenic mice was used. As shown in FIG. 16, this procedure yielded the purification of eGFP-ribosomal fusion protein along with cell specific mRNAs. The yield of total RNA from the initial IP is dependent on the number of labeled cells in the tissue and the intensity of the transgene expression within each cell, with RNA recoveries ranging from tens to thousands of nanograms per IP. RNA from the unbound (UB) fraction of the immunoprecipitation was harvested to measure the genes expressed in the dissected region as a whole. IP and UB mRNA were then amplified into labeled cRNA using standard protocols.

Labeled cRNAs from the IP and UB fractions were then hybridized onto Affymetrix mouse 430 2.0 microarrays. Samples derived from multiple independent pools of mice (replicates) were assayed for each cell type. FIG. 17 presents these data and illustrates that BACarray data are highly reproducible and cell-type specific

As shown in Panel A, replicates for the same cell type gave nearly identical genome wide translational profiles, confirming the results of Heiman et al, and extending this finding to many other cell types. The average Pearson's correlation between replicates for a given cell population from independently isolated samples was above 0.98 across all cell types. To determine whether the position of integration of the BACarray construct would influence the data, results obtained from independent BACarray founder lines prepared with the same engineered BAC were also examined, as presented in D. This analysis revealed that for independent founder lines targeting identical cell populations, the variation in translational profiles between lines was low, and no more extensive than that seen for replicate samples isolated from the same BACarray founder line (Panel D). Thus, the location of the transgene insertion into the genome had little global impact on the data obtained from the translating ribosome affinity purification (TRAP) methodology. Finally, four different monoclonal antibodies and one goat polyclonal against eGFP were tested. Each antibody immunoprecipitated comparable levels of mRNA for the BACarray lines tested; similar global gene translational profiles were obtained from each antibody tested in IPs from specific BACarray lines. A small number of probesets were consistently enriched in every BACarray dataset analyzed. Since these same probesets are also enriched in immunoprecipitates from control mice with no transgene expression, it was concluded that they represent background which have systematically eliminated from further analysis.

The enrichment for each mRNA immunoprecipitated from the targeted cell type (IP) versus its expression in the tissue sample dissected for the analysis (UB) was measured. The ratio of IP/UB was calculated, thereby identifying those genes which are highly enriched in each cell type. Panel B shows scatter plots for three representative cell types of the cerebellum. Differences are evident between the genome-wide translational profiles of IP samples compared to whole tissue (UB), with each cell population displaying a unique profile of thousands of specifically enriched genes on the microarray. As in Panel C, Venn diagrams constructed from the top 1000 most enriched probesets for each cell type can be used to illustrate this point. Thus, approximately 75% of these enriched probesets are not shared between cerebellar Purkinje cells, granule cells and unipolar brush cells, and only 52 of the probesets enriched in these three cell types versus whole cerebellum are shared between them. Furthermore, the primary data collected from these experiments has been deposited in the Gene Expression Omnibus (Edgar et al., 2002).

FIG. 18 presents the accuracy of this methodology to enrich for cell-specific genes. The BACarray data for known cell-specific markers (positive controls) for each cell type and the BACarray data for genes known to be expressed exclusively in other cell types (negative controls) were examined. Panel A shows a scatter plot of IP vs. UB for spinal cord motor neurons. Probesets for known markers of motor neurons with measurable signal on the array are clearly enriched in the IP sample, whereas probesets for glial cell-specific RNAs, that should not be present in these cells, are enriched in the UB sample. To establish the generality of this finding, the enrichment in the IP or UB sample was quantified by calculating an average ratio of IP/UB for positive and negative controls for each cell type where at least three positive controls could be found in the literature. As shown in Panel B, all IPs showed a clear enrichment for appropriate known markers, (Panel B, plotted in log base 2). Even for cell types with only one known marker (Pnoc positive interneurons, and Grp expressing unipolar brush cells), probesets for these genes were consistently and highly enriched in the IP. In the IPs with the lowest relative yield of RNA, such as those for mature oligodendrocytes (Panel B), and Cort expressing interneurons, background was proportionally higher, and enrichment was less robust.

Novel cell-specific markers for rare cell types using the BACarray approach were identified. Eleven genes predicted by BACarray to be enriched in either the Grm2 expressing interneurons of the granule cell layer (Golgi cells), or the Pnoc expressing cells of the cerebral cortex were screened. Using confocal microscopy, double immunofluorescence for both eGFP-L10a fusion protein and the ISH probes were evaluated. For the nine genes where ISH gave clear results, all were clearly overlapping with eGFP-L10a.

Panel C shows that in the case of cerebellar Golgi cells, there is a great deal of overlap between eGFP-L10a expression in the BACarray line and expression of the genes chosen for this analysis. This overlap confirms the specificity of the results obtained for this and other cell types. Nonetheless, the enrichment of a particular mRNA in the IP sample cannot be used to conclude that it is exclusively expressed in the cell type labeled in the BACarray transgenic line, or that it is expressed in all cells of that type. For example, the ISH databases (world wide web address: stjudebgem.org; world wide web address: brain-map.org) clearly indicate that Penk1 is expressed in scattered cells in both the granular and molecular layers of the adult cerebellum. Furthermore, as shown in panel 1, Penk1 mRNA does not appear to be expressed exclusively in those expressing Grm2. Finally, some mRNAs enriched in the BACarray data collected from Golgi cells were not detected using the fluorescence ISH technique, perhaps reflecting limited sensitivity of ISH for low expressed genes or the need for more rigorous probe design. Thus, although a clear result for Ceacam10 expression in granule layer interneurons is not evident in either of the ISH databases (world wide web address: stjudebgem.com; world wide web address: brain-map.org), in both cases one can see scattered signal in this area that may indicate expression of this mRNA in cerebellar Golgi neurons.

In order to further validate the BACarray datasets, the enrichment of a variety of mRNAs isolated from the Chat (motor neuron) and Pcp2 (Purkinje cell) BACarray transgenic lines were measured with quantitative real time PCR (qRT-PCR) (Panel D). For all of the control genes tested, this methodology confirmed the BACarray results. For genes not previously known to be expressed in a specific cell type, results from qRTPCR demonstrated that seven out of the eight mRNAs assayed were in fact cell type enriched (Panel D). Moreover, despite a negative ISH result, qRT-PCR validated the expression of Ceacam10 in the cerebellum and its enrichment in Golgi cells (Panel D). In some cases, therefore, the translating ribosome affinity purification (TRAP) methodology appears to be more sensitive than ISH.

2) Quantitative PCR (qPCR) of Purified mRNA

In some experiments, 20 ng of purified RNA was used to produce cDNA with a NuGEN WT Ovation kit (NuGEN Technologies, San Carlos, Calif.) and the resulting cDNA was purified and quantified. 10 ng of cDNA was used for each real-time gene expression assay. Applied Biosystems (Foster City, Calif.) TaqMan pre-designed gene expression assays were used, following the manufacturer's instructions and using an Applied Biosystems 7900 Sequence Detection System.

Alternatively, in some experiments, cDNA was synthesized from 20 ng of total RNA from the three replicate IP and UB samples using M-MulV reverse transcriptase (M0253L), from New England Biolabs (Ipswich, Ma), using oligo dT23VN (SEQ ID NO: 1) as a primer, then purified with the Qiagen Quick PCR cleanup, following manufacturer's instructions (Qiagen, Valencia, Ca).

Most primer sequences (Table 8) for qRT-PCR were obtained from Primer Bank (Wang and Seed, 2003). PCR was performed using Bio-Rad iQ syber green supermix following manufacturer's protocols (Biorad, Hercules, Calif.), with 500 nm final concentration of each primer. Cycling and quantitation were performed using Biorad iQ5 multiplex real-time detection hardware. PCR was carried out for 45 cycles (94°, 30 seconds, 63°, 30 seconds, 72°, 30 seconds), followed by a melt curve. Each replicate was assayed in triplicate. Conditions yielding dimers, as demonstrated by melt curve and/or gel electrophoresis were excluded from further analysis. Primers that did not yield product in at least 2 of 3 replicates prior to 35 cycles were excluded from further analysis. Data were normalized to B-actin (Overbergh et al., 1999) with the ddCT method, via iQ5's optical system software version 2, and averaged across replicates. All qPCR products were subcloned and sequenced to confirm accuracy of PCR. Microarray data were also normalized to B-actin for comparison purposes.

Table 8: List of qRT-PCR Primers

qRT-PCR primer sequences were mostly from PrimerBank (Wand and Seed, 2003) or literature. All are listed in 5′ to 3′ direction. Table 8 discloses the “Forward Primer” sequences as SEQ ID NOS 2-15 and the “Reverse Primer” sequences as SEQ ID NOS 16-29, all respectively, in order of appearance.

TABLE 8 List of qRT-PCR primers qRT-PCR primer sequences were mostly from PrimerBank (Wang and Seed, 2003) or literature. All are listed in 5′ to 3′ direction. Table 8 discloses the “Forward Primer” sequences as SEQ ID NOS 2-15 and the “Reverse Primer” sequences as SEQ ID NOS 16-29,  all repectively, in order of appearance. Gene Forward Primer Reverse Primer Primerbank ID or Ref B-Actin agagggaaatcgtgcgtgac caatagtgatgacctggccgt (Overbergh et al., 1999) Gfap gtaaagactgtggagatgcgggatggtga gtgctggtgtgggtgggaactgag Chat ccattgtgaagcggtttggg gccaggcggttgtttagataca 26338049a1 Slc18a3 gtgaagataggcgtgctatttgc gactgtggaggcgaacatgac 11096330a2 Cnp1 tgcttgatgataccaaccacg gctgggcacagtctagtcg 6753476a3 Pcp2 tgcagggcgatcggatggaggag tgaggggtgagcaggggttgagg Rilp ctgatgcggcaacctcagat ttgagcaagaacacgttggct 30185833a1 Popdc3 tgactgaacacccactctgc actgccacccataaaacctact 31745187a1 Kcnn1 ttgaaaagcgtaaacggctca cagagcaaaagagcagagtga 14161696a1 Foxq1 aaattggaggtgttcgtccca tccccgtctgagcctaagg 31560693a1 Cox7a1 gctctggtccggtcttttagc gtactgggaggtcattgtcgg 6753504a1 Tpm2 aagtcgctgatagcctcagag ggtctggtgtatctccacgttc 50190a1 Crygs cagacttccgctcgtacctaa tcgccctggggtaagatgt 6753532a1 Cfi cttggctctccacttgagttc ggagcgatgcgtgtatttctg 6671744a1

Example 7 Comparative Analysis of BACarray Data Collected from Many Cell Types

Previous examples show that the BACarray data accurately reflect expression of known positive and negative controls for each cell type and that these results can be confirmed by independent experimental analysis (Heiman et al.). This example illustrates the broad properties of these cells that could be inferred from comparative analysis of this large set of microarray data. The results of this analysis are presented in FIG. 19. A hierarchical clustering of the GCRMA normalized data from all 24 IP and 6 UB samples using the 20% of probesets with the highest coefficient of variation was performed, as presented in Panel A. This unsupervised clustering essentially recapitulates the known biology of CNS cell types. Thus, the three populations of cortical projection neurons are more similar to one another than they are to cortical interneurons, Purkinje cells, or motor neurons. Astroglial BACarray data collected from different regions of the brain are, as expected, more similar to one another and to Bergmann glia than they are to oligodendrocytes. Oligodendroglia are more similar to each other than they are to any neuronal population, etc. These findings support the concept that cells with similar gene expression patterns share similar functions, and suggest that analysis of BACarray data will allow for the identification of those gene products responsible for the distinguishing characteristics of each cell type.

The diversity of translational profiles across neuronal types nearly rivals the diversity between neurons and glia. Although related cell subtypes, such as different motor neurons or the Drd1 and Drd2 medium spiny neurons, are clearly tightly clustered, many neuronal types (e.g. Purkinje cells) are not strongly clustered with any other cell type. This suggests that comparative analysis of BACarray translational profiles obtained from highly specialized cell types may yield insights into their biochemical properties. Finally, individual cell types did not generally cluster tightly with the UB samples from their tissue of origin. In fact, profiles from UB samples derived from different brain regions were loosely clustered together relative to the data obtained from specific CNS cell types, suggesting that microarray data produced from dissected whole brain regions are less informative than BACarray analysis of individual cell types.

To examine this point in more detail, the microarray data from total cerebellum to that of the cerebellar cell types analyzed in this study were compared. As can be seen in Panel B, any single cell type has fewer probesets detectable than the whole cerebellar sample, since the whole cerebellar sample represents an aggregate of different cell types. However, comparative analysis of the sum of the probesets detectable in each of the six individual cerebellar cell types and the results obtained from whole cerebellar tissue reveals over 4000 probesets that are undetectable in the microarray from whole cerebellum. These undetectable probesets tend to represent cell-type enriched genes. In fact, for rare cell types, up to 42% of the genes enriched in that cell may not be detectable at all in whole-tissue microarray studies. For detection of genes expressed in specific cell types within complex brain regions, therefore, the translating ribosome affinity purification (TRAP) methodology can be more sensitive than microarray analysis of dissected brain regions.

The increased sensitivity of the translating ribosome affinity purification (TRAP) methodology results in identification of more mRNAs in each cell type, yielding a more complete picture of the translational profile for each cell type, more information. To assess if this increased sensitivity in fact does gives better information, the Shannon entropy was calculated for each probeset across the six whole tissue samples, and across the twenty four individual cell populations (Fuhrman et al., 2000; Shannon and Weaver, 1969). Shannon entropy is a measure of information content that describes the complexity of a signal across samples, with values ranging from 0 (low information) to 2 (high information). Data are presented in FIG. 20. Examples of probeset with low and high information are shown in Panel B. Shannon entropy measures of the information content in these samples reveals that the average Shannon entropy in cell type specific experiments (IP's) is over twice as high as that calculated from microarray data of whole tissue samples (t-Test, p<0.0001, average entropy across all IPs: 0.88+/−0.002, whole tissue: 0.41+/−0.003). This analysis demonstrates that microarray data collected from specific cell types using the BACarray strategy can provide better information than traditional microarray studies of dissected brain tissues.

As those genes with high entropy measures across all samples are those that vary in the most complex manner between cell types, this entropy measure was applied to assess what fundamentally determines the differences between cell types in the nervous system. As seen in Panel C, the ten percent of the probesets with highest entropy and those with the lowest entropy were classified with Gene Ontologies and then searched for functional categories that were over-represented (Ashburner et al., 2000; Maere et al., 2005). According to this analysis, cell type diversity in the nervous system is driven primarily by the expression of cell surface proteins, such as channels and receptors, and also to some extent by the specific expression of transcription factors and calcium binding proteins. Genes with less information content tend to be those that that are more ubiquitously expressed, such as ribosomal and mitochondrial proteins. This is not to say that they do not vary—their expression can range often from two to five fold across cell types—but they vary less dramatically than the tens to thousands fold changes of many receptors and channels.

Table 9 is a comparative analysis of translational profiling to identify co-regulated genes that could encode the highly specialized properties of individual cell types. This analysis is can be useful in trying to identify gene sets for known functions or candidate functions for novel gene products, since in many cases these cohorts of co-regulated genes will include genes with well known functions. To test whether the BACarray data provided can yield productive results in this sort of query, a probeset for a gene known to be involved in myelination was selected—the myelin basic protein (Mbp). Its highest correlates were examined across all IP and UB samples. In the top 35 genes correlating with Mbp expression (min correlation, 0.86), 6 genes also known to be involved in myelination were identified, including Plp1, Cnp, Mog, Mal and Mobp, and another three genes previously identified in a proteomic screen of myelin components (Table 9).

TABLE 9 Genes correlating with myelin basic protein. Probeset Correlation Symbol Name 1419646_a_at 1 Mbp myelin basic protein 1417275_at 0.968 Mal myelin and lymphocyte protein, T-cell differentiation protein 1451718_at 0.964 Plp proteolipid protein (myelin) 1440902_at 0.958 Galnt5 UDP-N-acetyl-alpha-D- galactosamine:polypeptide N-acetylgalactosaminyltransferase 5 1433785_at 0.958 Mobp myelin-associated oligodendrocytic basic protein 1436578_at 0.955 A330104H05Rik novel 1428792_at 0.946 Bcas1 breast carcinoma amplified sequence 1 1439506_at 0.934 C11ORF9 novel homolog 1426960_a_at 0.931 Fa2h fatty acid 2-hydroxylase 1433543_at 0.926 Anln anillin, actin binding protein (scraps homolog, Drosophila) 1448768_at 0.921 Mog myelin oligodendrocyte glycoprotein 1418086_at 0.917 Ppp1r14a protein phosphatase 1, regulatory (inhibitor) subunit 14A 1434094_at 0.914 6330530A05Rik novel 1420760_s_at 0.911 Ndrg1 N-myc downstream regulated 1 1447807_s_at 0.905 Plekhh1 pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 1425546_a_at 0.905 Trf transferrin 1436974_at 0.904 A230069A22Rik novel 1450241_a_at 0.904 Evi2a ecotropic viral integration site 2a 1418472_at 0.903 Aspa aspartoacylase (aminoacylase) 2 1452834_at 0.9 2600010E01Rik novel 1429909_at 0.894 4833411O04Rik novel 1453009_at 0.893 1110060I01Rik novel 1440813_s_at 0.89 Plxnb3 plexin B3 1437171_x_at 0.888 Gsn gelsolin 1423871_at 0.887 BC014795 novel 1434399_at 0.887 Galnt6 UDP-N-acetyl-alpha-D- galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 1435854_at 0.885 Tmem10 transmembrane protein 10 1416371_at 0.878 Apod apolipoprotein D 1418406_at 0.877 Pde8a cAMP-specific cyclic nucleotide phosphodiesterase PDE8 1416003_at 0.875 Cldn11 claudin 11 1434606_at 0.872 Erbb3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) 1451932_a_at 0.872 Tsrc1 thrombospondin repeat containing 1 1418980_a_at 0.871 Cnp1 cyclic nucleotide phosphodiesterase 1 1416318_at 0.87 Serpinb1a serine (or cysteine) proteinase inhibitor, clade B, member 1a 1424468_s_at 0.868 D330037A14Rik novel Correlation of genes with a Mbp probeset (1419646_a_at) identifies known and putative novel myelination genes. Four of the first eleven genes correlated with Mbp represent genes for the known myelin components Mal, Plp, Mobp, and Mog. This coexpression suggests that novel genes highly correlated with Mbp (C11orf9, A330104H05Rik, Bcas1) may also be involved in myelination. Pearson's correlation. Only the first probeset for each gene is shown for those genes with multiple probesets on the array. In bold are genes identified in an independent proteomic screen of myelin components (Vanrobaeys et al., 2005).

FIG. 21 shows a comparative analysis of the individual cell samples to identify only those genes most highly specific for each population. An iterative comparison was performed: one-by-one, each sample was compared to each other sample in the dataset, and for each population, probesets were sorted by their average ranking across these comparisons. Data were then combined and clustered by expression the top one hundred ranked probesets for each population in a heatmap (Panel A). This heat map readily illustrates the extent to which distinct cell types are characterized by specific cohorts of genes. For example, cerebellar Purkinje cells are clearly distinguished by a group of genes that are not seen in any other cell types (Panel A). Thus none of the top twenty five most specific probesets observed in the Purkinje cell sample are found in any of the top twenty five most specific probesets for any of the other cell types. In contrast, Drd1 and Drd2 medium spiny neurons, two closely related cell types, co-express many genes that are not found in the other cell populations analyzed, yet they also express distinct subsets of genes that differentiate them (Heiman et al). Thus, comparative analysis of BACarray data can be used to characterize CNS cell populations with unique biochemical and physiological properties, and to distinguish between closely related cell types at the molecular and biochemical level.

As shown in the tables in Panel B, the top twenty five most specific probesets in each cell type include probesets for both well known cell-specific markers and novel, previously uncharacterized genes. For example, Pcp2, the calcium binding protein Calb1, the scaffolding/synaptic protein Homer3, and the transcription factor Ebf2, all of which are known to be specifically expressed in Purkinje cells (Malgaretti et al., 1997; Shiraishi et al., 2004; Wang et al., 1997), are among the most highly ranked probesets in the Pcp2 BACarray list. Mobp, one of the most abundant components of the CNS myelin sheath (Montague et al., 2006), is prominent in the Cmtm5 myelinating oligodendrocytes' list. The expression of Tcrb in deep layer cortical neurons (Nishiyori et al., 2004) is confirmed in the Ntsr1 BACarray data. The large number of uncharacterized genes with cell specific translation identified here provide an important resource for discovery of novel biochemical pathways operating in these cell types, or for the identification of new proteins operating in well known pathways. Finally, comparative analysis can reveal discrepancies that are not apparent from anatomical studies. For example, the most specific probesets for the Etv1 line identify several genes well-known to be expressed in lymphoid cells, suggesting that in this line the eGFP-L10a transgene may also be expressed in circulating cells in the CNS vasculature. Taken together, the data shown above demonstrate two important strengths of large-scale comparative analyses of BACarray data. First, molecular relationships between cell types can be easily established with hierarchical clustering; second, groups of genes that encode the biochemical functions of specific cell types can be identified using this sort of systematic comparative approach.

Example 8 Analysis of BACarray Data Collected From Spinal Motor Neurons

Due to their involvement in a variety of serious neurological disorders and severe, acute injuries, spinal cord motor neurons (MN) are among the most well studied cell types of the CNS. As such, they provide an opportunity to evaluate the BACarray data with the depth of the knowledge available for this cell type. In particular, a wealth of anatomical and physiological data available for MN, and the comprehensive studies of transcription factors involved in their development, allowing comparison of the BACarray data presented here with the published literature. As shown in FIG. 22, in a single BACarray experiment, most of the MN expressed molecules that have been documented in prior studies are rediscovered. To perform this analysis, BACarray results were color coded as ‘expressed,’ ‘enriched,’ or ‘not expressed,’ as described in the methods. This classification was then compared to results reported in the adult rodent literature, color coded simply as either ‘expressed’ or ‘not expressed’ or left uncolored in cases where there were no studies or conflicting data. In most cases, where microarray probesets were present and informative, BACarray results agree well with the literature. Thus, it has been reported that MNs express glutamate receptors sensitive to AMPA, kainate, and NMDA (Rekling et al., 2000). These results suggest that the specific receptor subunits mediating these responses include Gria3 and 4, Grik2 and 4, and Grin1, 3a and 3b. Inhibition in MNs should be due the actions of the Glrα2 and GlrB glycine receptor subunits and both metabotropic (Gabbr1) and ionotropic GABAergic receptors, potentially composed of Gabrα2, α5, and β3 subunits. These data predict that MNs should respond to all classic neurotransmitters, including acetylcholine, via Chrnα4/β2 and/or Chrnα7 receptors, and serotonin, via the Htr1d receptor. In disagreement with prior immunohistochemical findings (Rekling et al., 2000), expression of Drd1 and or Drd2 in MNs was not detected. Moreover, transgenic mice for Drd1 and Drd2 do not show transgene expression in MNs, nor does the Allen Brain Atlas ISH show expression in brain stem MN, supporting the BACarray results.

MNs also express a variety of newly characterized receptors and orphan receptors. For example, BACarray data has successfully identified Grin3b as a MN specific gene encoding an NMDA subunit. This receptor was recently characterized as creating a unique glycine gated channel in MNs (Chatterton et al., 2002; Nishi et al., 2001). Several other genes enriched in MNs have also been identified which potentially encode for MN specific receptors that either have not been previously characterized in MNs or are entirely unstudied. Two that are particularly interesting are the vitamin D receptor and the orphan receptor P2rx11. Future studies investigating the role of these receptors in MN behavior may explain cases of reversible muscle weakness in patients with vitamin D deficiency (Whitaker et al., 2000; Ziambaras and Dagogo-Jack, 1997), or suggest new pathways important to MN function.

Example 9 Molecular Phenotyping and Translational Profiling of Cholinergic Motor Neurons and Purkinje Neurons

To determine whether the BACarray technique could be used for the characterization of other types of neurons, cholinergic and Purkinje cell-specific BACarray lines were produced, as presented in FIG. 23. The cholinergic cell BACarray line was produced by placement of the eGFP-L10a transgene under the control of the choline acetyltransferase (Chat) locus, which is specifically expressed in cholinergic cells in the CNS. As expected from the published Chat expression pattern in the rat CNS (Oh et al., 1992), the Chat BACarray line DW167 showed highest eGFP-L10a expression in cholinergic cells of the dorsal striatum and ventral striatum (nucleus accumbens, olfactory tubercle, and islands of Calleja), basal forebrain, brain stem, spinal cord, and medial habenula (Panel a). eGFP expression was shown to be restricted to cholinergic cells in all of these structures, including motor neurons of the brain stem (Panel b), by indirect immunofluorescence staining for Chat. One exception to this colocalization was in the pedunculopontine and laterodorsal tegmental nuclei, where only a minority of cholinergic cells were labeled with eGFP.

The Purkinje cell BACarray line DR166 was produced by placement of the eGFP-L10a transgene under the control of the Purkinje cell protein 2 (Pcp2) locus, which is specifically expressed in cerebellar Purkinje cells of the CNS (Oberdick et al., 1988). The Pcp2 BACarray line showed eGFP-L10a expression that was restricted to cells that possessed a characteristic Purkinje cell morphology (Panel c). Restriction of eGFP-L10a expression to cerebellar Purkinje cells was confirmed by indirect immunofluorescent staining of Calbindin-D28K (Panel D), which in the cerebellum is specifically expressed in Purkinje cells (Nordquist et al., 1988). Array data were collected from brain stem cholinergic motor neurons using the Chat BACarray line and from Purkinje cells using the Pcp2 BACarray line. Replicate Chat BACarray samples gave nearly identical genome-wide translational profiles (average Pearson correlation=0.982), as was the case for replicate Pcp2

BACarray samples (average Pearson correlation=0.997). To provide a measure of the enrichment of each mRNA immunoprecipitated from the targeted cell type (IP) versus its expression in a reference sample (unbounds in immunoprecipitation), the ratio of the expression in each IP sample versus the reference sample was calculated. This comparison identified those genes which were highly enriched in each cell type versus a common reference sample. As expected from the analysis of D1 and D2 combined BACarray data versus whole brain minus, differences are evident between the genome-wide translational profiles of IP samples compared to the reference sample for all the individual samples analyzed: striatonigral (D1), striatopallidal (D2), brain stem cholinergic (Chat), and Purkinje cells (Pcp2). FIG. 24 shows that enrichment of cell-specific positive-control genes and exclusion of known negative-control genes (glial genes), were evident for each comparison (panels a-d). Venn diagrams constructed from the top 1,000 enriched probesets from this analysis (Tables 17-20) confirmed that the translational profiles of striatonigral and striatopallidal cells show greater similar to each other than they do to the translational profiles of either brain stem cholinergic cells or Purkinje cells (panels e-h).

Example 10 Molecular Phenotyping and Translational Profiling of the Striatum

1) Overview

This example presents a study of the specific properties of the striatum. This example illustrates one embodiment that can uncover unexpected molecular and physiological complexity in closely related and spatially adjacent CNS cell populations. The striatum is a subcortical part of the telencephalon. It is the major input station of the basal ganglia system. In this example, transgenic mice containing molecularly tagged ribosomal proteins in the striatonigral and striatopallidal cells of the striatum were created using standard BACarray techniques. Striatonigral and striatopallidal medium spiny neurons (MSNs), are intermixed, indistinguishable in somato-dendritic morphology, and of major interest due to their role in the etiology of various neurological diseases, including Parkinson's disease, schizophrenia, attention deficit hyperactivity disorder, drug addiction, and Huntington's disease. Striatonigral MSNs send projection axons directly to the output nuclei of the basal ganglia, i.e. the substantia nigra and the internal segment of the globus pallidus (the entopeduncular nucleus in rodents), while striatopallidal MSNs send projection axons to the external segment of the globus pallidus. Striatonigral MSNs are known to preferentially express the dopamine D1 receptor (Drd1; Drd1a) and the neuropeptides substance P (Tac1) and dynorphin (Pdyn), while striatopallidal MSNs preferentially express the dopamine D2 receptor (Drd2), the adenosine A2a receptor (Adora2a) and the neuropeptide enkephalin (Penk) (S. Gong et al., Nature 425, 917-25 (2003)).

2) Production of BACarray Striatal Mice

Mice containing molecularly tagged ribosomal proteins in the striatonigral and striatopallidal cells of the striatum were created. Homologous recombination in bacteria was used to place eGFP-L10a under the control of either the D2 receptor (striatopallidal) or D1 receptor (striatonigral) loci in the appropriate BACs. Mouse lines were generated by pronuclear injection of engineered BACarray DNA contracts into fertilized oocytes.

Mouse lines were screened by immunohistochemistry for appropriate expression of the transgene, as judged by known D1 and D2 receptor expression patterns. In the Drd2 line, 75.8% colocalization of pro-enkephalin with eGFP-L10a (n=204/269) was observed and in the Drd1a line 0% colocalization was observed (n=0/325). For the Drd2 line, this number is likely an underestimate, as heat-induced epitope retrieval in citrate buffer is needed for optimal staining using pro-enkephalin antibodies. Heat-induced epitope retrieval in the colocalization experiments was not utilized, however, because both eGFP fluorescence and the ability of various GFP antibodies to recognize eGFP are substantially diminished upon use of these epitope retrieval methods. Colocalization data for eGFP-L10a (direct eGFP fluorescence) and pro-enkephalin expression (immunohistochemical staining) is presented (FIG. 25): (a) Immunohistochemistry to eGFP in adult sagittal sections from the D2 BACarray line CP101. (b) Characterization of D2 BACarray line CP101 striatal MSN cells: direct eGFP fluorescence (left panel with high-magnification image insert); enkephalin immunohistochemical staining (middle panel); merge (right panel, with 20 μm scale bar). (c) Immunohistochemistry to eGFP in adult sagittal sections from the D1 BACarray line CP73. (d) Characterization of D1 BACarray line CP73 striatal MSN cells: direct eGFP fluorescence (left panel); enkephalin immunohistochemical staining (middle panel); merge (right panel).

The D2 BACarray line showed highest transgenic eGFP-L10a expression in the dorsal and ventral striatum, olfactory tubercle, and hippocampus. In addition, expression of eGFP was seen in the substantia nigra pars compacta and ventral tegmental area of this line, as expected due to D2 autoreceptor expression in dopaminergic cells (panel a). The D1 BACarray line showed highest transgenic eGFP-L10a expression in the dorsal and ventral striatum, olfactory bulb, olfactory tubercle, and cortical layers 5 and 6 (panel c). As expected for a ribosomal protein fusion, direct visualization of eGFP fluorescence revealed localization of transgenic eGFP-L10a to the nucleoli and cytoplasm (panel b). eGFP co-localization with enkephalin expression (striatopallidal cell marker) was observed in striatal cells from the D2 BACarray line but not the D1 BACarray line (panels b and d), verifying correct BAC-mediated cell-type expression.

The polysome profile from D2 BACarray mouse striatal extract is presented in FIG. 26 as follows: Top: Post-mitochondrial striatal extract (S20) was loaded onto a linear sucrose gradient (20-50% w/w). After velocity sedimentation, fractions (direction of sedimentation noted by arrow) were collected as UV absorbance (254 nm) was measured. Bottom: gradient fractions were ethanol precipitated, resuspended in SDS-PAGE loading buffer, and Rpl7 and eGFP-L10a content were assayed by Western blotting. Velocity sedimentation analysis of polysome complexes isolated from striatal extracts for both lines of BACarray mice confirmed incorporation of the eGFP-L10a fusion protein into functional polysomes in vivo.

3) BACarray Profiling of Striatonigral and Striatopallidan MSNs

A plurality of differentially expressed genes can be identified which characterize striatonigral and striatopallidal MSNs, including all previously known markers. Translation profiling and molecular Phenotyping was performed with immunoaffinity-purified mRNA from adult striatonigral or striatopallidal BACarray mice. Following two rounds of in vitro transcription, biotin-labeled antisense RNA (cRNA) was used to interrogate Affymetrix GeneChip Mouse Genome 430 2.0 arrays. For each cell type, data were collected from three independent biological replicates, each prepared from a cohort of 7 animals. Analysis of immunoaffinity-purified samples revealed no bias for mRNA length or abundance (FIG. 27). Lengths of transcripts were based on all available mouse curated RefSeq RNA sequences (file transfer protocol address ncbi.nih.gov/genomes/M_musculus/RNA). Where multiple transcript variants for a single gene were available, the longest one was chosen. RefSeq lengths were plotted against D1 (a) or D2 (b) BACarray IP normalized expression values. There was no correlation observed between transcript length and IP values. Striatal expression values for all Affymetrix Genechip probe sets were obtained by total RNA arrays from wild type striatal tissue (data not shown). These values were plotted against (c) D1 BACarray or (d) D2 BACarray IP normalized values. As expected, higher expression in total striatum (no IP, wild type mice) correlates with higher D1 or D2 BACarray IP values. The few genes that showed modest expression in total striatum but had low IP values include known non neuronal genes.

Comparative analysis of these data revealed that all 8 of the well-characterized, differentially expressed MSN markers were enriched using the BACarray approach: D2 (Drd2) (36.6×), adenosine 2a receptor (Adora2a) (13.2×), and enkephalin (Penk) (7.5×) were enriched in the striatopallidal BACarray sample, while D1 (Drd1a) (3.9×), substance P (Tac1) (3.6×), and dynorphin (Pdyn) (5.6×) were enriched in the striatonigral BACarray sample (FIG. 28 and

Table 10). Four striatopallidal-enriched mRNAs (Adk, Plxdc1, BC004044, and Hist1h2bc) and six striatonigral-enriched mRNAs (Slc35d3, Zfp521, Ebf1, Stmn2, Gnb4, and Nrxn1) were confirmed as reported in a microarray study of FACS sorted MSNs (S. Magdaleno et al., PLoS Biol 4, e86 (2006)). However, the data identified approximately 70 additional striatopallidal enriched transcripts and over 150 additional striatonigral-enriched transcripts (

Table 10). To provide an initial test of the data, quantitative PCR studies were carried out using independent biological BACarray D1 and D2 samples and a different cDNA amplification procedure (A. Alexa, J. Rahnenfuhrer, T. Lengauer, Bioinformatics 22, 1600-7 (2006)). Differential expression of Eya1, Isl1, Gng2, and Crym in striatonigral MSNs, and Gpr6, Lhx8, Gpr88, Trpc4, and Tpm2 in striatopallidal MSNs was confirmed (Table 11 and Table 12). qPCR assays used were Gapdh: Mm99999915_g1, Drd2: Mm00438541_m1, Gpr6: Mm01701705_s1, Lhx8: Mm00802919_m1, Gpr88: Mm02620353_s1, Trpc4: Mm00444284_m1, Tpm2: Mm00437172_g1, Eya1: Mm00438796_m1, Tac1: Mm00436880_m1, Isl1: Mm00627860_m1, Gng2: Mm00726459_s1, Chrm4: Mm00432514_s1, Drd1a: Mm02620146_s1, Crym: Mm01281258_m1, Actb: Hs99999903_m1, and Fth1: Hs01694011_s1. Each assay was performed in quadruplicate and fold enrichment values were derived from the comparative Ct method (following Applied Biosystems recommendations), with each target amplification compared to a Gapdh or Actb reference amplification.

TABLE 10 Genes differentially translated between striatonigral and striatopallidal cells. Probe Symbol GenBank FCH 1457424_at Eya1 BB760085 32.49 1424606_at Cplx3 BC024854 19.56 1418047_at Neurod6 NM_009717 13.05 1428393_at Nrn1 AK003046 12.21 1417560_at Sfxn1 BB478992 11.99 1422720_at Isl1 BQ176915 10.10 1450723_at Isl1 BQ176915 9.44 1420388_at Prss12 NM_008939 9.25 1419473_a_at Cck NM_031161 9.11 1447863_s_at Nr4a2 BB322941 9.00 1419200_at Fxyd7 NM_022007 7.90 1455034_at Nr4a2 BB703394 7.52 1417559_at Sfxn1 BB478992 7.42 1428642_at Slc35d3 AK018094 7.32 1451499_at Cadps2 AF000969 6.87 1450750_a_at Nr4a2 NM_013613 6.44 1426852_x_at Nov X96585 6.44 1431339_a_at Efhd2 AK007560 6.00 1428184_at 3110035E14Rik BB348639 5.61 1416266_at Pdyn AF026537 5.59 1428157_at Gng2 AV021455 5.08 1441388_at BB428710 5.05 1437478_s_at Efhd2 AA409309 5.04 1423280_at Stmn2 BM115022 4.89 1428156_at Gng2 AV021455 4.85 1421727_at Eya1 NM_010164 4.84 1451322_at Cmbl BC024580 4.82 1423281_at Stmn2 BM115022 4.73 1448293_at Ebf1 BB125261 4.72 1416711_at Tbr1 NM_009322 4.70 1422256_at Sstr2 NM_009217 4.68 1416302_at Ebf1 BB125261 4.55 1440361_at BB272510 4.48 1455893_at Rspo2 BG067392 4.35 1428664_at Vip AK018599 4.35 1419470_at Gnb4 BI713933 4.32 1452779_at 3110006E14Rik AK014009 4.26 1416301_a_at Ebf1 BB125261 4.25 1436312_at Ikzf1 AV317621 4.20 1424737_at Thrsp BC009165 4.16 1448366_at Stx1a NM_016801 4.14 1451620_at C1ql3 BB768838 4.11 1448889_at Slc38a4 NM_027052 4.08 1426851_a_at Nov X96585 4.07 1426318_at Serpinb1b AF426025 4.03 1423287_at Cbln1 AA016422 4.01 1417343_at Fxyd6 AB032010 3.99 1427017_at Satb2 BB104560 3.92 1419469_at Gnb4 BI713933 3.89 1455629_at Drd1a BE957273 3.87 1417400_at Rai14 NM_030690 3.74 1423286_at Cbln1 AA016422 3.71 1456051_at Drd1a BB282271 3.69 1419184_a_at Fhl2 NM_010212 3.64 1434440_at Gnai1 BQ174580 3.64 1418789_at Sntg2 NM_133742 3.61 1416783_at Tac1 NM_009311 3.61 1457440_at Sstr4 BB451927 3.59 1418451_at Gng2 BB522409 3.57 1442724_at AW060763 AW120464 3.55 1455758_at Prkcc BM215011 3.52 1430306_a_at Atp6v1c2 AK004157 3.48 1456515_s_at Tcfl5 AV044715 3.43 1416953_at Ctgf NM_010217 3.40 1441917_s_at Tmem40 BB468188 3.35 1437091_at Accn4 AV323885 3.30 1436786_at Sec14l3 AV024133 3.29 1429274_at 2310010M24Rik AK009282 3.23 1421017_at Nrg3 NM_008734 3.23 1416503_at Lxn NM_016753 3.22 1417933_at Igfbp6 NM_008344 3.18 1427510_at Gnai1 U38501 3.16 1436862_at Doc2a BB543070 3.13 1452065_at Vstm2 BB085570 3.11 1448546_at Rassf3 BB703307 3.08 1429175_at Tmem178 AK014196 3.08 1418417_at Msc NM_010827 3.08 1448812_at Hpcal1 NM_016677 3.07 1454691_at Nrxn1 BB336165 3.04 1450042_at Arx BB322201 3.04 1446498_at Il20ra BB551879 3.03 1457984_at Crh BM933756 3.03 1438729_at BB176347 BB331017 3.03 1440108_at Foxp2 BM964154 2.99 1434695_at Dtl AV270035 2.97 1439632_at BQ174397 2.96 1417288_at Plekha2 NM_031257 2.93 1426937_at 6330406I15Rik AK018128 2.92 1438624_x_at Hs3st2 BB494451 2.85 1440849_at 6330417G04Rik AV327187 2.85 1426738_at Dgkz BC014860 2.84 1428781_at Dmkn BI452905 2.83 1439789_at Ebf1 BQ177189 2.83 1450055_at Vsnl1 NM_012038 2.83 1417569_at Ncald BG071381 2.80 1437671_x_at Prss23 BB378796 2.79 1444661_at Gpr26 BB247791 2.79 1427975_at Rasl10a AK008807 2.77 1438112_at 9430021M05Rik AA546727 2.76 1436787_x_at Sec14l3 AV024133 2.73 1417579_x_at Gmppa NM_133708 2.72 1456146_at 2210411A11Rik BI735554 2.72 1436503_at BC048546 BF302511 2.71 1420720_at Nptx2 NM_016789 2.70 1455925_at Prdm8 AV349236 2.68 1448632_at Psmb10 NM_013640 2.67 1426329_s_at Baalc AF371320 2.65 1448669_at Dkk3 AK004853 2.65 1425090_s_at Kcnc4 BC024837 2.64 1454959_s_at Gnai1 BQ174580 2.63 1453558_at 4930504H06Rik AK015697 2.63 1423757_x_at Igfbp4 BC019836 2.62 1437405_a_at Igfbp4 BB787243 2.59 1450059_at Fancg BG072083 2.54 1450708_at Scg2 NM_009129 2.52 1429269_at BC068157 BE992549 2.51 1422537_a_at Id2 NM_010496 2.51 1417568_at Ncald BG071381 2.49 1435411_at BE950834 2.49 1424966_at Tmem40 BC019416 2.48 1438796_at Nr4a3 BE692107 2.48 1416776_at Crym NM_016669 2.47 1437406_x_at Igfbp4 BB787243 2.45 1423202_a_at Ncor1 U22016 2.45 1444307_at AW491448 2.44 1439573_at Rtn4rl2 BE992565 2.43 1417605_s_at Camk1 NM_133926 2.42 1425784_a_at Olfm1 D78264 2.39 1455074_at Efcab1 AV307860 2.39 1451342_at Spon1 BC020531 2.38 1420955_at Vsnl1 NM_012038 2.38 1417192_at Tomm70a NM_138599 2.38 1452169_a_at Dgkz BC014860 2.37 1418304_at Pcdh21 NM_130878 2.37 1454770_at Cckbr AV221910 2.37 1440918_at AW495511 2.37 1448754_at Rbp1 NM_011254 2.37 1428240_at Nrxn1 AK017578 2.36 1453245_at 9130024F11Rik BB329046 2.36 1438231_at Foxp2 AV322952 2.36 1438232_at Foxp2 AV322952 2.35 1417578_a_at Gmppa NM_133708 2.34 1428077_at Tmem163 AK011522 2.32 1453317_a_at Khdrbs3 AK014353 2.32 1419551_s_at Stk39 BG919998 2.32 1421144_at Rpgrip1 NM_023879 2.32 1421926_at Mapk11 AV329330 2.30 1415849_s_at Stmn1 BC010581 2.30 1424763_at 1700027N10Rik BC019423 2.27 1459847_x_at Gfra2 AV354240 2.27 1449314_at Zfpm2 NM_011766 2.26 1430634_a_at Pfkp BB076574 2.26 1457032_at Ak5 BB546359 2.25 1453129_a_at Rgs12 AK004813 2.24 1447669_s_at Gng4 AV347903 2.24 1433909_at AW048713 2.22 1424701_at Pcdh20 BB528056 2.21 1435959_at Arhgap15 BM246535 2.21 1417988_at Resp18 NM_009049 2.21 1455885_at Gna12 AV238106 2.21 1427054_s_at Abi3bp BC026627 2.20 1458112_at Adarb2 BB527550 2.20 1427115_at Myh3 M74753 2.19 1449556_at H2-T23 NM_010398 2.18 1435176_a_at Id2 BF019883 2.16 1422552_at Rprm NM_023396 2.15 1416131_s_at C920006C10Rik BB188557 2.15 1436609_a_at Lrpap1 AV309553 2.14 1451332_at Zfp521 BC021376 2.13 1452444_at Napb AU067119 2.13 1457277_at BC038925 AI314927 2.13 1434243_s_at Tomm70a AV233564 2.12 1436592_at BB453760 2.12 1448249_at Gpd1 BC019391 2.11 1417604_at Camk1 NM_133926 2.09 1443129_at BB363699 2.09 1452741_s_at Gpd2 BQ175968 2.08 1417960_at Cpeb1 NM_007755 2.08 1418726_a_at Tnnt2 NM_011619 2.08 1442887_at Gpr39 BQ175060 2.07 1421176_at Rasgrp1 BB354696 2.06 1428650_at Tns1 AK003780 2.06 1428323_at Gpd2 BQ175968 2.06 1425287_at Zfp189 BC021326 2.06 1452974_at Nol8 AK017551 2.05 1417038_at 8-Sep NM_017380 2.03 1417943_at Gng4 NM_010317 2.03 1448326_a_at Crabp1 NM_013496 2.03 1416407_at Pea15 AI323543 2.03 1436821_at Plcxd3 BB293136 2.02 1426606_at Crtac1 BB426194 2.01 1449429_at Fkbp1b NM_016863 2.01 1452731_x_at LOC554327 BM195235 2.00 1425854_x_at Tcrb-V13 U07661 2.00 1452936_at Crtac1 AV341285 1.99 1448660_at Arhgdig NM_008113 1.98 1415897_a_at Mgst1 BI150149 1.98 1431429_a_at Arl4a AK006286 1.98 1416004_at Ywhah NM_011738 1.97 1418982_at Cebpa BC011118 1.97 1418271_at Bhlhb5 NM_021560 1.96 1449403_at Pde9a NM_008804 1.95 1449799_s_at Pkp2 AA516617 1.94 1454745_at Arhgap29 BG074320 1.94 1425051_at Isoc1 AK010892 1.94 1455324_at Plcxd2 BQ176176 1.94 1418792_at Sh3gl2 AF326561 1.93 1437390_x_at Stx1a AV339210 1.93 1416133_at C920006C10Rik BB188557 1.93 1455443_at Gdap1l1 BB733286 1.93 1433664_at Ube2q2 BM202586 1.92 1431749_a_at Rasgrp1 AK013548 1.91 1455374_at BB332873 1.91 1426766_at 6330403K07Rik AK018106 1.91 1445081_at A930041I02Rik BB335888 1.90 1455458_x_at Gmppa AU044197 1.89 1436182_at BG092481 1.88 1425484_at Tox BB547854 1.87 1456954_at Kcna6 BM119753 1.84 1417434_at Gpd2 NM_010274 1.83 1454708_at Ablim1 BG065289 1.83 1416008_at Satb1 AV172776 1.81 1449839_at Casp3 BG070529 1.80 1420858_at Pkia AK010212 1.79 1425006_a_at Vrk1 BC016676 0.57 1437573_at BF018351 0.57 1456434_x_at Hspb8 BB764222 0.56 1452879_at Synpo2 AI848603 0.55 1436238_at Lgi3 AI841179 0.54 1431320_a_at Myo5a AK002362 0.54 1448845_at Rpp25 NM_133982 0.53 1428574_a_at Chn2 AK006398 0.53 1416967_at Sox2 U31967 0.53 1445461_at BB096245 0.53 1426572_at Me2 BM235734 0.53 1423171_at Gpr88 BE947345 0.53 1423424_at Zic3 BB732077 0.52 1451236_at Rerg BC026463 0.52 1420533_at Gucy1a3 AK004815 0.52 1419754_at Myo5a NM_010864 0.52 1455265_a_at Rgs16 BB100249 0.52 1419717_at Sema3e NM_011348 0.52 1436946_s_at Gng5 AV216608 0.51 1419738_a_at Tpm2 AK003186 0.51 1439526_at Me2 AV375160 0.51 1458951_at Vrk1 AV341598 0.51 1441368_at BB102769 0.50 1455753_at C630035N08Rik BQ176181 0.50 1436994_a_at Hist1h1c BB533903 0.49 1428283_at Cyp2s1 AK004699 0.49 1420694_a_at Dach1 BB522228 0.49 1454821_at B3gat1 BB424673 0.49 1452540_a_at Hist1h2bp M25487 0.48 1456935_at AI606473 AI606473 0.48 1419879_s_at Trim25 AA960166 0.48 1426215_at Ddc AF071068 0.48 1420534_at Gucy1a3 AK004815 0.48 1418072_at Hist1h2bc NM_023422 0.47 1426573_at Me2 BM235734 0.47 1437231_at Slitrk6 AV246497 0.47 1456858_at Gpr149 BB075339 0.47 1442226_at Sema3e AV348197 0.47 1448839_at Ankrd47 NM_030697 0.46 1422673_at Prkcm NM_008858 0.46 1443365_at Htr4 BB431008 0.46 1438841_s_at Arg2 AV002218 0.46 1434141_at Gucy1a3 BG072799 0.46 1448415_a_at Sema3b NM_009153 0.45 1439614_at Htr4 BB308379 0.45 1436913_at Cdc14a BB479310 0.45 1451033_a_at Trpc4 BB271442 0.45 1449241_at Klhl1 NM_053105 0.45 1460327_at Gpr88 BE947345 0.45 1448162_at Vcam1 BB250384 0.44 1446681_at BB086117 AV327329 0.44 1450992_a_at Meis1 AW547821 0.44 1449577_x_at Tpm2 AK003186 0.44 1417688_at BC004044 NM_030565 0.44 1435138_at Tmem28 AV016797 0.43 1457648_x_at BC004044 BB084182 0.42 1417306_at Tyk2 NM_018793 0.42 1424854_at Hist1h4i BC019757 0.41 1443827_x_at BC004044 BB375974 0.41 1418895_at Skap2 NM_018773 0.41 1443372_at BB417900 0.41 1440484_at Unc5d BB525782 0.41 1448276_at Tspan4 NM_053082 0.40 1425028_a_at Tpm2 BC024358 0.39 1447623_s_at Prkcm AV297026 0.39 1455739_at EG245190 BB279146 0.39 1418355_at Nucb2 NM_016773 0.38 1419517_at Cnih3 NM_028408 0.38 1423477_at Zic1 BB361162 0.38 1422860_at Nts NM_024435 0.38 1423367_at Wnt7a AK004683 0.37 1435479_at BM055476 0.36 1437156_at Efcbp1 BB392041 0.36 1452135_at Gpx6 AV001252 0.34 1460623_at Skap2 BB753881 0.34 1417577_at Trpc3 NM_019510 0.33 1439566_at Gprin3 BB245373 0.33 1418639_at Sftpc NM_011359 0.33 1457142_at Efcbp1 BB667731 0.32 1439627_at Zic1 AV031691 0.31 1454659_at Dctd BG069699 0.30 1427300_at Lhx8 D49658 0.30 1416319_at Adk NM_134079 0.29 1438292_x_at Adk BB559878 0.28 1451191_at Crabp2 BC018397 0.26 1419577_at A530089I17Rik NM_133999 0.26 1440177_at 9.63E+17 BM899529 0.26 1422586_at Ecel1 NM_021306 0.25 1433787_at Nell1 AI841091 0.23 1422541_at Ptprm NM_008984 0.22 1434458_at Fst BB444134 0.21 1427519_at Adora2a BG311385 0.18 1433578_at Slc10a4 BE824538 0.17 1421365_at Fst NM_008046 0.15 1424902_at Plxdc1 AF378760 0.13 1427038_at Penk1 M13227 0.13 1428547_at Nt5e AV273591 0.13 1460244_at Upb1 NM_133995 0.11 1440148_at Gpr6 AI852874 0.08 1460710_at Adora2a BB748999 0.08 1418950_at Drd2 NM_010077 0.03 1435211_at Ttc12 BB554351 0.00 Fold-change values (FCH) are given relative to D1 (striatonigral) cells. Probe = Affymetrix probe identification; Symbol = official gene symbol.

TABLE 11 Real time PCR analysis of D1 enriched messages: range from replicates. Fold enrichment in D1 Gene versus D2 cell type Eya1 19.85-21.51 Tac1 (known) 10.97-11.87 Isl1 10.06-10.87 Gng2 5.91-6.43 Chrm4 (known) 5.02-5.57 Drd1a (known) 2.96-3.4  Crym 1.57-1.88

TABLE 12 Real time PCR analysis of D2-enriched messages: range from replicates. Fold enrichment in D2 versus Gene D1 cell type Drd2 (known) 49.79-51.55 Gpr6 12.30-12.81 Lhx8 3.57-4.06 Gpr88 1.94-2.13 Trpc4 1.93-2.25 Tpm2 1.84-1.96

4) Large Scale Validation of BACarray Profiling of Striatonigral and Striatopallidan MSNs

Large-scale validation of the data using publicly available gene expression databases was carried out. Data were pooled from D1 and D2 BACarray experiments to represent MSN-enriched subtypes and compared to data collected from the total RNA of one whole brain (minus striatum) (Table 13).

The analysis resulted in detection of several thousand translated mRNAs enriched in striatum relative to whole brain, including all previously-known striatal-enriched genes: Ppp1r1b/Darpp-32 (S. I. Walaas, P. Greengard, JNeurosci 4, 84-98 (January, 1984),) Ptpn5/Step (P. J. Lombroso, J. R. Naegele, E. Sharma, M. Lerner, J Neurosci 13, 3064-74 (July, 1993),) Arpp-19 (J. A. Girault, A. Horiuchi, E. L. Gustafson, N. L. Rosen, P. Greengard, J Neurosci 10, 1124-33 (April, 1990),) Arpp-21/RCS (C. C. Ouimet, H. C. Hemmings, Jr., P. Greengard, J Neurosci 9, 865-75 (March, 1989),) Goal/Golf (D. Herve eta!., J Neurosci 13, 2237-48 (May, 1993),) Rhes/Rasd2 (J. D. Falk et al., J Neurosci Res 57, 782-8 (Sep. 15, 1999),) Rgs9 (S. J. Gold, Y. G. Ni, H. G. Dohlman, E. J. Nestler, J Neurosci 17, 8024-37 (Oct. 15, 1997),) Adcy5 (C. E. Glatt, S. H. Snyder, Nature 361, 536-8 (Feb. 11, 1993),) Gng7 (J. B. Watson et al., J Neurosci Res 39, 108-16 (Sep. 1, 1994),) Rasgrp2 (H. Kawasaki et al., Proc Natl Acad Sci USA 95, 13278-83 (Oct. 27, 1998),) Pde1b (J. W. Polli, R. L. Kincaid, Proc Natl Acad Sci USA 89, 11079-83 (Nov. 15, 1992),) Pde10a (K. Fujishige, J. Kotera, K. Omori, Eur J Biochem 266, 1118-27 (December, 1999),) Gpr88 (K. Mizushima et al., Genornics 69,314-21 (Nov. 1, 2000),) Rarb (W. Krezel, P. Kastner, P. Chambon, Neuroscience 89, 1291-300 (1999),) and Strn4 (F. Castets et al., J Cell Biol 134, 1051-62 (August, 1996)) as well as the transcription factors Foxp1, Foxp2 (R. J. Ferland, T. J. Cherry, P. O. Preware, E. E. Morrisey, C. A. Walsh, J Comp Neuro1460, 266-79 (May 26, 2003),) Ebfl (S. Magdaleno et al., PLoS Biol 4, e86 (2006)), and ZJP503/Nolz (C. W. Chang et al., Proc Natl Acad Sci USA 101, 2613-8 (Feb. 24, 2004).) (Table 13).

As independent confirmation of these data, mRNA expression patterns were examined for a subset of the candidate MSN-expressed genes in the GENSAT/Brain Gene Expression Map (BGEM) and Allen Brain Atlas (ABA) in situ hybridization (ISH) databases (world wide web address: ncbi.nlm.nih.gov/projects/gensat/; world wide web address: stjudebgem.org/) Only those genes for which expression data were available in both gene expression atlases were chosen. The data are presented in FIG. 29. Of the first 100 genes appearing in the MSN-enriched dataset, 26 were present in both the BGEM and ABA public ISH atlases. Enriched striatal expression is evident for 22 of these genes (Table 13); panel a).

ISH data were available in the GENSAT/BGEM and ABA for 16 genes appearing between numbers 1,000 to 1,100 on the MSN enriched list. In this case, striatally-enriched expression is apparent for 7 of these genes ((Table 13); panel b).

Expression analysis in sagittal sections of genes which were amongst the top 100 (a) or 1,000 1,100 (b) genes identified in the study as MSN enriched, with the rank order of each gene noted below the gene name. Non redundant gene ranking was calculated using the highest ranked probeset corresponding to each gene, with redundant probesets eliminated. Left panel, in situ hybridization images taken from the Allen Brain Atlas (Allen Brain Atlas [Internet]. Seattle (Wash.): Allen Institute for Brain Science.© 2006. Available from: world wide web address: brain map.org.; E. S. Lein et al., Nature 445, 168-76 (Jan. 11, 2007)); right panel, in situ hybridization images taken from the Brain Gene Expression Map (BGEM) database (world wide web address: stjudebgem.org/) (S. Magdaleno et al., PLoS Biol 4, e86 (April, 2006). Allen Brain Atlas images all correspond to adult brain; BGEM images all correspond to adult brain except for the following, for which the oldest available data were postnatal day 7 (P7): Drd2, Ppp1r1b, Dlx6, Gdnf, Bcl11b, Foxg1, Limd2, Fem1b, Dynll1, Atbf1, Foxo3a, Dnalc4, Mtmr7, Dnmt3a.

TABLE 13 Genes differentially translated between medium spiny neurons (MSNs) and the rest of the brain. Probe Symbol GenBank FCH 1420437_at Indo NM_008324 198.43 1427343_at Rasd2 BC026377 149.45 1457132_at BF456117 133.95 1458342_at Tmem90a BB313069 108.01 1418950_at Drd2 NM_010077 85.64 1438355_at Tmem90a AI414870 79.94 1441368_at BB102769 71.65 1426814_at AU024582 BM248309 57.27 1418782_at Rxrg NM_009107 56.75 1423171_at Gpr88 BE947345 52.53 1457143_at LOC665139 BB322292 50.59 1426438_at Ddx3y AA210261 49.33 1419696_at Cd4 NM_013488 45.51 1458499_at Pde10a AW123977 41.50 1427344_s_at Rasd2 BC026377 34.92 1450213_at Pde7b NM_013875 33.08 1455190_at Gng7 BM114283 31.37 1429285_at Serpina9 AK009343 29.48 1454906_at Rarb BB266455 28.73 1443287_at Gm1337 BB555669 28.56 1428642_at Slc35d3 AK018094 28.48 1417680_at Kcna5 NM_008419 28.33 1451331_at Ppp1r1b BC026568 28.25 1442558_at Ptprn2 BB180191 27.93 1454867_at Mn1 BB234631 27.26 1437750_at Tmem158 BI133257 26.54 1457919_at D030054H15Rik BB446049 25.08 1427807_at ENSMUSG00000060559 BC017159 24.97 1436566_at Rab40b AV364488 24.65 1456818_at Stk32a BB279083 24.52 1452507_at Dlx6 AF022078 24.32 1457651_x_at Rem2 BB270375 24.03 1428781_at Dmkn BI452905 23.79 1460710_at Adora2a BB748999 23.75 1455629_at Drd1a BE957273 23.51 1422079_at Prkch NM_008856 22.36 1435296_at Adra2c AV349563 21.79 1440148_at Gpr6 AI852874 20.60 1437935_at 4930486G11Rik BB821151 19.59 1427523_at Six3 AI509029 19.13 1419389_at Pde10a BQ180352 19.09 1454581_at 5330425B07Rik AK019908 19.03 1440166_x_at Htr1d BB378627 18.87 1426815_s_at AU024582 BM248309 18.68 1419080_at Gdnf NM_010275 17.98 1435211_at Ttc12 BB554351 17.93 1438022_at Rab11fip3 BQ266518 17.69 1445539_at BE687857 17.64 1449420_at Pde1b NM_008800 17.44 1427683_at Egr2 X06746 16.79 1456640_at Sh3rf2 AW910872 16.61 1456280_at Clspn BG067086 16.53 1451236_at Rerg BC026463 16.46 1460262_a_at 1700037H04Rik NM_026091 16.39 1460038_at Pou3f1 BG065255 16.23 1443694_at Rgs20 BB794177 16.06 1417804_at Rasgrp2 NM_011242 16.01 1451280_at Arpp21 BB159263 15.99 1450339_a_at Bcl11b NM_021399 15.73 1449977_at Egr4 NM_020596 15.61 1457424_at Eya1 BB760085 15.60 1441000_at EG237749 BB076832 15.52 1454043_a_at Kcnab1 AK015412 15.48 1438841_s_at Arg2 AV002218 15.44 1417210_at Eif2s3y NM_012011 15.28 1429229_s_at 4930534B04Rik BE980134 15.28 1454762_at Nox1 BM932447 15.26 1419390_at Pde10a BQ180352 15.22 1435366_at D430042O09Rik BB486367 15.19 1425756_at Rab40b AF425643 15.18 1418744_s_at Tesc NM_021344 14.99 1416627_at Spint1 NM_016907 14.90 1436650_at Filip1 AV241894 14.84 1460136_at AW047481 AI462839 14.68 1435808_at A230051G13Rik BB174377 14.53 1421140_a_at Foxp1 BG962849 14.38 1437698_at Myo5b AV370579 14.36 1416456_a_at Chia BC011134 14.31 1442166_at Cpne5 BB273427 14.21 1456051_at Drd1a BB282271 13.84 1421365_at Fst NM_008046 13.82 1420496_at F12 NM_021489 13.79 1436092_at BB336256 13.75 1448327_at Actn2 NM_033268 13.69 1429228_at 4930534B04Rik BE980134 13.65 1419826_at Kif17 AW492270 13.61 1436734_at E130309F12Rik BB523550 13.60 1421353_at Pde7b NM_013875 13.54 1425833_a_at Hpca AF326551 13.53 1454656_at Spata13 AV271736 13.50 1455298_at Id4 BB306828 13.39 1425363_at B4galnt1 BC022180 13.23 1439854_at Hrk BQ175572 13.22 1434008_at Scn4b BE993937 13.11 1417812_a_at Lamb3 NM_008484 13.10 1435221_at Foxp1 BM220880 13.06 1429617_at Cyld BM119209 12.96 1418357_at Foxg1 NM_008241 12.81 1436532_at Dcamkl3 BB326709 12.78 1441468_at BB326028 12.77 1447861_x_at Mrg1 AV329643 12.66 1447766_x_at Limd2 AV003249 12.63 1454877_at Sertad4 BQ174721 12.63 1418847_at Arg2 NM_009705 12.57 1422720_at Isl1 BQ176915 12.56 1417704_a_at Arhgap6 NM_009707 12.45 1455361_at Dgkb AW493391 12.28 1432073_at 1700113I22Rik AK007198 12.27 1418743_a_at Tesc NM_021344 12.18 1444734_at A330001L22Rik BB183877 12.17 1433815_at Jakmip1 AV290082 12.08 1452217_at Ahnak BE570050 12.02 1452135_at Gpx6 AV001252 11.98 1460325_at Pum1 BB837171 11.96 1439618_at Pde10a AI448308 11.94 1434521_at Rfxdc2 BB148972 11.92 1435285_at Mpped2 BB731805 11.92 1416266_at Pdyn AF026537 11.84 1422230_s_at Cyp2a5 NM_007812 11.82 1422706_at Tmepai AV370981 11.81 1448468_a_at Kcnab1 AF033003 11.74 1434917_at Cobl BQ173923 11.68 1418881_at Efcbp2 NM_054095 11.68 1433743_at Dach1 BG075820 11.67 1441733_s_at Nup153 C88147 11.66 1442077_at Htr1b BB197581 11.66 1433566_at Rasl10b BB381618 11.54 1423117_at Pum1 BB837171 11.50 1426439_at Ddx3y AA210261 11.48 1436216_s_at 2610204M08Rik BM234799 11.46 1431751_a_at Mpped2 AK012553 11.40 1437865_at Spata13 AW546433 11.40 1418691_at Rgs9 NM_011268 11.39 1442058_s_at Psmc3ip BE687992 11.38 1419033_at 2610018G03Rik AW556821 11.36 1455564_at Bcr BQ176236 11.36 1455156_at Strn BG519214 11.35 1434664_at 2410129H14Rik BI153133 11.33 1415953_s_at Mark2 BI686265 11.31 1417129_a_at Mrg1 U68384 11.26 1436275_at Kcnip2 AW490636 11.23 1456515_s_at Tcfl5 AV044715 11.15 1453222_at 4833427B12Rik BE863648 11.04 1425870_a_at Kcnip2 AF439339 10.91 1420666_at Doc2b BM117900 10.75 1459941_at Asph BB096900 10.73 1448913_at Smarcd1 NM_031842 10.71 1440741_at Htr1d BB829587 10.68 1457038_at Frem2 BM201912 10.66 1458406_at AI429294 BG144063 10.63 1430686_at 4833418N02Rik AK014719 10.62 1446877_at C230014O12Rik BB381622 10.52 1441928_x_at Ell BB139475 10.44 1416350_at Klf16 NM_078477 10.40 1444978_at BM223267 10.36 1417356_at Peg3 AB003040 10.34 1431386_s_at Mbtps1 AK002809 10.32 1421943_at Tgfa M92420 10.27 1435553_at Pdzd2 AV376136 10.17 1427931_s_at Pdxk BG063905 10.16 1427682_a_at Egr2 X06746 10.09 1444681_at Erc2 BB749686 10.07 1422153_a_at Asb11 NM_026853 10.00 1439566_at Gprin3 BB245373 9.99 1420146_at Tfb1m AI429207 9.98 1449957_at Ptprv NM_007955 9.95 1426241_a_at Scmh1 AB030906 9.94 1424208_at Ptger4 BC011193 9.86 1450026_a_at B3gnt2 AV306734 9.85 1436738_at Pif1 AV094878 9.75 1434248_at Prkch BM243756 9.75 1422068_at Pou3f1 NM_011141 9.75 1448374_at Med28 AK005130 9.72 1456760_at Centg3 BB342676 9.70 1422813_at Cacng1 NM_007582 9.69 1449425_at Wnt2 BC026373 9.64 1438799_at Dlx6os1 AV338180 9.63 1456283_at Neto1 AV346211 9.57 1425351_at Srxn1 BC011325 9.57 1424576_s_at Cyp2c44 BC025819 9.56 1423544_at Ptpn5 BB188812 9.53 1433923_at Krt77 AV230775 9.53 1430640_a_at Prkar2b BI695530 9.52 1427779_a_at Cd4 U75219 9.51 1452966_at 9130430L19Rik AK020296 9.46 1425130_a_at Ptpn5 U28216 9.41 1440711_at C630001G18Rik BB426320 9.37 1431936_a_at Neu2 AK009828 9.36 1434025_at Klf5 BG069607 9.33 1441867_x_at 4930534B04Rik AI480494 9.30 1427912_at Cbr3 AK003232 9.27 1436002_at BB484128 9.23 1449502_at Dazl NM_010021 9.21 1430612_at 1810033B17Rik BB533148 9.19 1455242_at Foxp1 BM220880 9.18 1441105_at BE980314 9.16 1447017_at BE956696 9.03 1435334_at Ttc7 BG866904 8.97 1436998_at Ankrd43 BB428991 8.96 1454086_a_at Lmo2 AK013416 8.87 1419542_at Dazl NM_010021 8.82 1442234_at Chst2 BB770422 8.72 1449331_a_at Rapsn NM_009023 8.68 1442021_at Mppe1 BB502545 8.65 1429201_at Cyld AK013508 8.55 1440435_at Ky BB126077 8.55 1441350_at Fgf3 AV302620 8.53 1434519_at Ddah1 AW556888 8.53 1452298_a_at Myo5b AW546331 8.51 1422407_s_at Hras1 NM_008284 8.45 1416996_at Tbc1d8 BC005421 8.43 1428873_a_at 4121402D02Rik AW495537 8.37 1422448_at Tff2 NM_009363 8.36 1443036_at Zfp804a BG073535 8.35 1417522_at Fbxo32 AF441120 8.32 1453799_at 9430038I01Rik AK020460 8.31 1438224_at Zswim5 BE572265 8.28 1430864_at Ttll9 AK015740 8.25 1451008_at St8sia3 BB360510 8.25 1453330_at 0610010D24Rik AK002458 8.24 1420872_at Gucy1b3 BF472806 8.22 1444383_at 1110014J01Rik AV347845 8.21 1424132_at Hras1 BC011083 8.20 1420695_at Dach1 BB522228 8.13 1438266_at Adamtsl5 BB764453 8.09 1456054_a_at Pum1 BB314559 8.08 1422157_a_at Itgb1bp1 NM_008403 8.06 1420961_a_at Ivns1abp NM_028582 8.05 1424899_at Nmnat3 BC005737 7.97 1424409_at Cldn23 BC019534 7.94 1435071_at Zfyve1 AV327165 7.93 1437353_at Setd1b BB494182 7.87 1456830_at Ppp1r2 BB542221 7.87 1445606_a_at 2900009J06Rik BB663189 7.87 1435227_at BM117007 7.85 1446455_at BB520649 7.85 1443676_at Slc4a4 AV339935 7.83 1429316_at Rasgef1a AK018120 7.82 1438417_at Pwwp2 BB809239 7.82 1434996_at Slc25a16 AV316233 7.79 1422621_at Ranbp2 BM507707 7.79 1452176_at Nup153 BB292874 7.78 1437460_x_at Rin1 BB264363 7.78 1447642_x_at Dmwd AV354897 7.70 1417341_a_at Ppp1r2 NM_025800 7.69 1431349_at Hnrpab AK013709 7.65 1438441_at Id4 AI323288 7.64 1416242_at Klhl13 NM_026167 7.64 1441963_at Ubox5 BB427703 7.64 1422678_at Dgat2 AK002443 7.60 1447359_at Zfp575 AI326876 7.58 1450183_a_at Sh2b3 NM_008507 7.58 1451517_at Rhobtb2 AF420001 7.55 1437017_at AI480653 BB046727 7.54 1455296_at Adcy5 BE952286 7.52 1422779_at Smpd3 NM_021491 7.49 1434531_at Mgat5b BB400317 7.46 1450072_at Ash11 BG694892 7.45 1422335_at Adra2c NM_007418 7.45 1417062_at Armc10 NM_026034 7.45 1426454_at Arhgdib AK002516 7.38 1443073_at LOC545681 BB355954 7.38 1458407_s_at AI429294 BG144063 7.36 1453323_at 2900079G21Rik AK013806 7.34 1438664_at Prkar2b BB216074 7.27 1428839_at Wdr53 AK005105 7.27 1442708_at BB504418 7.26 1418467_at Smarcd3 NM_025891 7.25 1455401_at Camkk2 AW061083 7.24 1434732_x_at Tomm7 AV044898 7.24 1429618_at Cyld BM119209 7.23 1428860_at 4930572J05Rik AK019809 7.22 1453264_at Marveld3 AK007346 7.22 1421142_s_at Foxp1 BG962849 7.22 1418714_at Dusp8 NM_008748 7.20 1452077_at Ddx3y AA210261 7.19 1428370_at 1500011B03Rik AK018264 7.19 1454832_at Phactr1 BG228702 7.19 1434153_at Shb BI408715 7.18 1456475_s_at Prkar2b BB216074 7.15 1422722_at 1700001K19Rik NM_025488 7.14 1434495_at Patz1 BM208058 7.14 1423532_at Rnf44 AI850285 7.12 1437091_at Accn4 AV323885 7.11 1418017_at Pum2 BI689507 7.10 1423049_a_at Tpm1 AK002271 7.10 1455080_at Ppp1r16b BB375209 7.10 1416239_at Ass1 NM_007494 7.10 1428338_at Spata2L AK019166 7.07 1425104_at Kctd1 BC022615 7.06 1433627_at Sec23ip AW546839 7.02 1455085_at 1700086L19Rik BI526033 7.02 1416804_at Ehbp111 NM_053252 7.00 1452729_at Dpm3 BE446919 7.00 1419234_at Helb BG070273 7.00 1449261_at Pbx2 NM_017463 6.99 1456336_at A330102K23Rik BB182912 6.99 1416983_s_at Foxo1 AI462296 6.99 1442220_at BB313387 6.97 1455961_at AV174022 6.96 1460567_at Rfxdc2 BB148972 6.94 1437579_at Nek2 C77054 6.94 1452913_at Pcp4l1 AV337888 6.93 1441223_at 3-Mar BB260801 6.93 1435504_at Clip4 BM217861 6.93 1434077_at Wdr37 AV222037 6.91 1452879_at Synpo2 AI848603 6.90 1460285_at Itga9 NM_133721 6.90 1424796_at 1700054N08Rik BC024705 6.89 1435125_at BB120497 BB303627 6.87 1433962_at 6720458F09Rik BB131965 6.86 1429098_s_at Nhej1 AK006481 6.85 1452411_at Lrrc1 BG966295 6.85 1422159_at Ppef2 BC027049 6.83 1452166_a_at Krt10 AK014360 6.83 1416981_at Foxo1 AI462296 6.82 1437092_at Clip4 AV042829 6.81 1430306_a_at Atp6v1c2 AK004157 6.80 1421442_at Flt4 NM_008029 6.77 1435649_at Nexn BM225804 6.75 1455683_a_at Tbc1d8 BB451404 6.73 1434649_at Ccm2 BI903794 6.72 1455804_x_at Oxct1 AV213379 6.71 1423344_at Epor AK010968 6.69 1460555_at 6330500D04Rik BM242294 6.68 1436799_at Rpl23a BB386177 6.67 1426687_at Map3k3 BG175594 6.66 1434032_at Centg3 BQ175381 6.65 1423530_at Stk32c BB320288 6.63 1443543_at BE980616 6.63 1447100_s_at C80506 6.62 1437842_at Plcxd1 BB311508 6.59 1420534_at Gucy1a3 AK004815 6.59 1455301_at Wipf3 BG064092 6.59 1429637_at 2210419I08Rik AK008987 6.57 1442051_at Hist2h3c1 BE691662 6.54 1434458_at Fst BB444134 6.54 1435605_at Actr3b BB125424 6.53 1422034_a_at Palm NM_023128 6.53 1419742_at 1700037H04Rik NM_026091 6.52 1438171_x_at Mettl9 BB056666 6.52 1448807_at Hrh3 NM_133849 6.50 1437097_at Zbed3 BB703866 6.49 1424759_at Arrdc4 BC017528 6.49 1438210_at Gpr149 BB126999 6.48 1441340_at Cep68 BF018298 6.47 1443887_at Ankrd13c BE132758 6.47 1422608_at Arpp19 BE648432 6.45 1452917_at Rfc5 AK011489 6.43 1416982_at Foxo1 AI462296 6.43 1418894_s_at Pbx2 NM_017463 6.42 1449158_at Kcnk2 NM_010607 6.42 1448978_at Ngef NM_019867 6.41 1427196_at Wnk4 BG074348 6.41 1424507_at Rin1 BC011277 6.40 1441089_at Eif2c1 BB205493 6.40 1427293_a_at Auts2 AK012475 6.39 1457195_at Plekhm1 BB206527 6.38 1436930_x_at Hmbs BB000512 6.37 1434359_at 6330500D04Rik AV329070 6.37 1420444_at Slc22a3 NM_011395 6.37 1417440_at LOC675933 NM_033566 6.37 1425503_at Gcnt2 AB037596 6.36 1438784_at Bcl11b BB329234 6.34 1460044_at Onecut2 BB389395 6.34 1439576_at BB325333 6.34 1428777_at Spred1 AK017680 6.34 1430413_at Tmem29 AK018228 6.34 1422293_a_at Kctd1 NM_134112 6.33 1423488_at Mmd BC021914 6.33 1436645_a_at Cnot4 BB066603 6.32 1445404_at Kif27 BB054454 6.32 1428547_at Nt5e AV273591 6.32 1454752_at Rbm24 AV307961 6.29 1452092_at 4631426J05Rik AK019474 6.28 1447830_s_at Rgs2 BB034265 6.27 1455447_at D430019H16Rik BM116882 6.26 1452904_at 1700026L06Rik BQ175881 6.26 1444363_at BB269445 6.26 1437839_x_at Mrpl11 AV209741 6.25 1445385_at BB551855 6.25 1419247_at Rgs2 AF215668 6.24 1416886_at C1d NM_020558 6.21 1419378_a_at Fxyd2 NM_052823 6.20 1450366_at Hrk NM_007545 6.19 1428495_at 2410003K15Rik BF300229 6.19 1439844_at 8430426J06Rik BE628126 6.18 1455345_at Phf15 BI663145 6.16 1449304_at 2310061J03Rik NM_133677 6.16 1435564_at C230078M08Rik BB547893 6.16 1451819_at Zswim6 BC021311 6.16 1449141_at Fblim1 BG070068 6.15 1448860_at Rem2 NM_080726 6.15 1450835_a_at Gfra4 NM_020014 6.15 1435096_at Ric8b BB667093 6.15 1454901_at Ypel2 BG069663 6.15 1423270_at Nedd4l BB663717 6.14 1452202_at Pde2a BG069616 6.12 1442754_at C030013G03Rik BE692283 6.10 1449863_a_at Dlx5 NM_010056 6.09 1422134_at Fosb NM_008036 6.09 1430222_at 9130007G19Rik BB538672 6.09 1434648_a_at Ccm2 BI903794 6.09 1428139_at Tmem180 AK015988 6.08 1439947_at Cyp11a1 C87524 6.08 1421141_a_at Foxp1 BG962849 6.08 1415976_a_at Carhsp1 AU080787 6.07 1433988_s_at C230098O21Rik BG075755 6.06 1422705_at Tmepai AV370981 6.06 1443334_at D430042O09Rik BB487579 6.06 1434909_at Rragd BF462770 6.04 1456930_at Camsap1 BE989461 6.03 1416605_at Nola2 BC024944 6.03 1417090_at Rcn1 NM_009037 6.02 1460686_at Cntd1 BC006866 6.02 1430190_at 1700041C02Rik AK006668 6.02 1430616_at 4930528A17Rik BB185833 6.01 1419248_at Rgs2 AF215668 5.99 1417524_at Cnih2 NM_009920 5.97 1453260_a_at Ppp2r2a AK010380 5.95 1443612_at Tmem16c AW123199 5.95 1424248_at Arpp21 BB159263 5.93 1449843_at St8sia2 BG071333 5.93 1420391_at Pard3 NM_033620 5.93 1434115_at Cdh13 BQ176681 5.92 1457052_at AW536275 BG064867 5.92 1456838_at BC072620 BE995608 5.92 1436538_at Ankrd37 AV084342 5.91 1438226_at AU022252 AV349089 5.90 1422208_a_at Gnb5 BC016135 5.89 1452368_at Bcr AI853148 5.88 1416488_at Ccng2 U95826 5.87 1435222_at Foxp1 BM220880 5.87 1432579_at Rshl2b AA544511 5.85 1417658_at Tbrg4 NM_134011 5.85 1422923_at Fgf3 NM_008007 5.85 1421037_at Npas2 BG070037 5.85 1431711_a_at 9030409G11Rik AK018497 5.85 1431242_at 6330575P09Rik BE995561 5.85 1452911_at Spred1 AK017680 5.84 1458133_at BB360008 5.81 1438019_at Ippk BM196347 5.81 1417003_at 0610012G03Rik BC021536 5.80 1448231_at Fkbp5 U16959 5.80 1428074_at Tmem158 BE981853 5.80 1456401_at Cacnb2 BB078175 5.79 1423376_a_at Dok4 AV341904 5.77 1443365_at Htr4 BB431008 5.76 1416087_at Ap1s1 NM_007457 5.76 1429104_at Limd2 AK012581 5.76 1435218_at Rasgef1a BI134758 5.75 1454742_at Rasgef1b BB003229 5.75 1437948_x_at Eif3s6ip BB443362 5.75 1447640_s_at Pbx3 BB554874 5.75 1460718_s_at Mtch1 AF192558 5.74 1453972_x_at Pparbp AK005207 5.74 1430520_at Cpne8 AW548480 5.71 1418015_at Pum2 BI689507 5.70 1455701_at Snx26 BI965517 5.67 1436160_at Krt26 BB150142 5.67 1458025_at Ttc7 BB519333 5.66 1427005_at Plk2 BM234765 5.66 1434682_at Zfp770 AV347367 5.64 1439291_at BB354792 5.64 1438680_at Auts2 BB429147 5.63 1443552_at E230008N13Rik BM932485 5.63 1453089_at 3110079O15Rik AA259365 5.62 1440501_at AU041480 BG072095 5.62 1440940_at Cacnb1 BE949510 5.62 1446840_at Thrap1 BQ032894 5.62 1460327_at Gpr88 BE947345 5.57 1426230_at Sphk2 AK016616 5.55 1419130_at Deadc1 NM_025748 5.54 1434967_at Zswim6 BM938007 5.53 1432782_at 4933435C09Rik AK017064 5.52 1441967_at Pddc1 BB467791 5.51 1433468_at 6430527G18Rik BB770958 5.51 1435741_at Pde8b BB312125 5.49 1437400_at Nedd41 BB309512 5.48 1460206_at Grasp NM_019518 5.47 1433767_at 1110018G07Rik AV257687 5.45 1420918_at Sgk3 BB768208 5.45 1438189_s_at Rai16 BB492312 5.44 1433507_a_at Hmgn2 BE553881 5.42 1450930_at Hpca AK002992 5.42 1418373_at Pgam2 NM_018870 5.40 1427278_at Clip4 AK017914 5.40 1430013_at Git1 BE993118 5.39 1437586_at Cnot4 BB756908 5.38 1439821_at Lrp2bp BB075157 5.38 1453268_at Thg11 AV224102 5.37 1451483_s_at 1700054N08Rik BC024705 5.35 1436725_at E130306D19Rik BM226118 5.34 1425123_at BC025816 BC025816 5.34 1442434_at D8Ertd82e BM195829 5.32 1444379_at Pwwp2 BB318408 5.31 1416558_at Melk NM_010790 5.30 1439855_at Tmtc1 AV341449 5.30 1427045_at Synpo AI849322 5.30 1450480_a_at Gprk6 AF040749 5.30 1427015_at C230021P08Rik BI732921 5.30 1417829_a_at Rab15 NM_134050 5.29 1457632_s_at Mrg1 BB207647 5.29 1453040_at 2810402A17Rik AK013345 5.28 1445459_at Sstr5 BB023293 5.27 1455670_at BQ175884 5.27 1424475_at Camkk2 BI157430 5.26 1424020_at Arl6ip6 BB837198 5.26 1444522_at BM942961 5.26 1434124_x_at 2400001E08Rik AV100071 5.26 1436100_at Sh2d5 AV347995 5.25 1437453_s_at Pcsk9 AV010795 5.24 1431665_a_at Timm8b AK004190 5.23 1420752_at Dtx3 NM_030714 5.23 1453165_at Mettl4 BB701076 5.22 1454256_s_at 1700113I22Rik AK004415 5.22 1427954_at BC048403 BC028785 5.21 1450227_at Ankrd6 BM199504 5.21 1454309_at 2810002N01Rik BB646622 5.20 1435941_at Rhbdl3 BM118307 5.20 1450723_at Isl1 BQ176915 5.19 1419006_s_at Peli2 NM_033602 5.18 1434301_at D330050I23Rik BE303700 5.18 1423442_a_at Fbxw2 BE854125 5.18 1434828_at B430201A12Rik BM200103 5.18 1431717_at 3526401B18Rik AK014386 5.18 1439028_at Ufm1 AI415161 5.17 1444615_x_at Runx1t1 AV327778 5.17 1434775_at Pard3 AW543460 5.17 1437980_at 9130230N09Rik BB814947 5.16 1455923_at Kctd8 BB352927 5.16 1455200_at Pak6 BB818370 5.15 1416479_a_at Tmem14c NM_025387 5.15 1452964_at Ttll11 AK016577 5.15 1422440_at Cdk4 NM_009870 5.15 1420694_a_at Dach1 BB522228 5.15 1456197_x_at BB342782 5.14 1418592_at Dnaja4 NM_021422 5.14 1421234_at Tcf1 NM_009327 5.13 1425753_a_at Ung BC004037 5.13 1426964_at 3110003A17Rik AK013984 5.13 1420778_at Tas1r3 NM_031872 5.12 1433610_at AA986860 BB522283 5.12 1422807_at Arf5 NM_007480 5.11 1447559_at AI834762 AI834762 5.10 1434014_at Atg4c BB291836 5.10 1433642_at Arl15 BB384173 5.09 1426573_at Me2 BM235734 5.08 1422165_at Pou3f4 X66603 5.08 1431186_at Dlg5 BF140264 5.08 1429422_at 4933412E12Rik AK016788 5.08 1453309_at 9330179D12Rik BB749938 5.08 1457512_at BB167650 5.08 1435615_at Zfp365 BB277790 5.08 1437378_x_at Scarb1 BB224405 5.07 1429124_s_at Rexo1 BQ178367 5.07 1455153_at Zfp236 BQ177220 5.07 1430617_at Oip5 BB238604 5.06 1427519_at Adora2a BG311385 5.06 1436142_at 3526401B18Rik BM218877 5.06 1438068_at BB251859 5.04 1429678_at 5730508B09Rik AK017758 5.04 1455022_at Strn BG070684 5.03 1448684_at Ppp1r2 NM_025800 5.03 1423639_at Hrh2 AK020259 5.02 1451998_at Tasp1 BC024597 5.02 1460166_at Rit2 BB271919 5.02 1451604_a_at Acvrl1 BC014291 5.02 1450418_a_at Yipf4 NM_026417 5.01 1453000_at Camsap1l1 AK005444 5.01 1438665_at Smpd3 BF456582 5.01 1432184_a_at 2610204M08Rik AK011884 5.01 1428095_a_at Tmem24 AK004920 5.01 1427745_x_at Prpmp5 X63005 5.01 1423802_at Camkv BC017634 5.00 1417897_at Brms1 NM_134155 5.00 1458967_at BM939341 5.00 1420950_at Znrf1 BB026596 4.99 1418783_at Trpm5 AF228681 4.99 1438613_at Kcna4 BB131475 4.98 1433996_at Suv39h2 BB296443 4.97 1435423_x_at 4933433P14Rik AV281088 4.96 1417082_at Anp32b NM_130889 4.96 1441883_at 0610010O12Rik AV092359 4.95 1456225_x_at Trib3 BB508622 4.95 1434154_at Kctd13 BQ177107 4.95 1423489_at Mmd BC021914 4.94 1452476_at Cacnb2 W41214 4.94 1447844_at AV077281 4.93 1433668_at Pnrc1 BI410130 4.93 1416398_at Mesdc1 BB474887 4.93 1423260_at Id4 BB121406 4.92 1440108_at Foxp2 BM964154 4.92 1430439_at Mctp1 AK013379 4.91 1440426_at Nfatc2 BB427970 4.91 1437181_at Peli2 BM121149 4.89 1422474_at Pde4b BM246564 4.89 1455033_at B430201A12Rik BB325849 4.88 1430519_a_at Cnot7 AK007767 4.88 1433451_at Cdk5r1 BB177836 4.88 1423202_a_at Ncor1 U22016 4.88 1455754_at Lmo3 BQ043839 4.87 1425400_a_at Cited4 BC025116 4.87 1422737_at Ncoa3 NM_008679 4.87 1442363_at 1110012J17Rik BB461471 4.86 1428698_at Dpp8 BM939621 4.86 1450071_at Ash11 BG694892 4.86 1447711_x_at 4933412E12Rik BB265147 4.85 1450975_at Cacng4 BB333636 4.84 1451898_a_at Sema6c AF363972 4.84 1426644_at Tbc1d20 BC002196 4.83 1428872_at 4121402D02Rik AW495537 4.82 1417393_a_at C1qdc2 NM_026125 4.82 1453314_x_at 2610039C10Rik AK012533 4.80 1450700_at Cdc42ep3 BB012489 4.80 1455178_at Rutbc1 BB496468 4.80 1424611_x_at Trub2 BG064045 4.79 1435083_at Ctxn1 BI155559 4.78 1439422_a_at C1qdc2 AV048291 4.78 1437707_at Slmo1 BB835597 4.77 1452533_at Ryr3 X83934 4.77 1455114_at Ccnu BB547482 4.77 1439031_at Jph4 BB376821 4.76 1451696_at Zfp64 BC004695 4.76 1424201_a_at Seh11 AW537349 4.76 1436733_at E130309F12Rik BB523550 4.75 1449241_at Klhl1 NM_053105 4.75 1435435_at Cttnbp2 BB357580 4.74 1451867_x_at Arhgap6 AF177664 4.73 1453551_at Polq AK020790 4.73 1436563_at 4932441J04Rik AV277283 4.73 1417530_a_at Srp9 BI661964 4.72 1428357_at 2610019F03Rik AK011462 4.72 1424117_at BC056474 BC024937 4.72 1452063_at 2410081M15Rik AK010736 4.72 1430729_at Myg1 AK017955 4.71 1435763_at Tbc1d16 BI218684 4.71 1417893_at Sfxn3 NM_053197 4.70 1460610_at 9430057O19Rik BG143491 4.70 1420094_at Hnrpdl AU015266 4.69 1426676_s_at Tomm70a BB225670 4.69 1431520_at 4933406J09Rik AK016696 4.69 1425080_at Zfp286 BE651907 4.68 1428279_a_at Atxn7l4 AK013145 4.68 1453781_at AK009527 4.68 1436013_at Gsg11 AI852434 4.67 1441879_x_at Mkrn1 AV218897 4.67 1454572_at 2810414N06Rik AK013093 4.67 1435585_at Tceal7 BB378019 4.67 1441051_at Trim27 BB401251 4.67 1420580_at 4930429B21Rik NM_026249 4.66 1420446_at Odf3 NM_027019 4.66 1450306_at Zp1 NM_009580 4.66 1437069_at Osbpl8 BG969333 4.66 1423835_at Zfp503 BB447914 4.66 1416397_at Mesdc1 BB474887 4.65 1425154_a_at Csf1 M21149 4.65 1426893_at C230093N12Rik BC023470 4.64 1426032_at Nfatc2 AF289078 4.63 1453577_at 2610018I03Rik BB399635 4.63 1439888_at BB271581 4.63 1436421_s_at Arpc51 BB403233 4.63 1432312_a_at 4931440B09Rik AK016509 4.62 1423831_at Prkag2 BB756794 4.62 1439246_x_at Tnrc6a BB822587 4.62 1420533_at Gucy1a3 AK004815 4.61 1418169_at Zcchc14 BB223737 4.61 1443542_at BB767151 4.60 1428676_at Tmprss6 AK004939 4.60 1437146_x_at Coro7 AV025980 4.60 1428535_at 9430020K01Rik AK004276 4.59 1418173_at Krt25 NM_133730 4.59 1443888_at AU023762 BB426608 4.59 1438327_at Zfp533 BE981788 4.59 1433937_at Trp53bp2 BB814564 4.58 1454958_at Gsk3b BQ175580 4.58 1420667_at Doc2b BM117900 4.58 1448922_at Dusp19 NM_024438 4.57 1453008_at 2300002D11Rik AK009004 4.57 1418375_at Mbd6 NM_033072 4.56 1417443_at Acot11 NM_025590 4.56 1425328_at BC008163 AU067643 4.56 1456601_x_at Fxyd2 AV002675 4.54 1454845_x_at Mchr1 AW049955 4.54 1425327_at BC008163 AU067643 4.54 1455772_at Pgr BB428874 4.54 1450649_at Gng10 NM_025277 4.54 1435966_x_at Mrpl13 BE953095 4.54 1457088_at Pldn BB780781 4.54 1432541_at 4930408O17Rik AK015114 4.53 1422642_at Cdc42ep3 BB012489 4.53 1416606_s_at Nola2 BC024944 4.53 1423438_at Kptn AK009746 4.53 1420871_at Gucy1b3 BF472806 4.52 1417372_a_at Peli1 BC016515 4.52 1455170_at 2810001G20Rik BB250227 4.51 1438889_at BB183456 4.51 1460058_at BE648595 4.51 1427674_a_at Sez6 D29763 4.51 1440204_at Foxg1 AW494150 4.50 1423699_at Ncaph2 BC003900 4.50 1443372_at BB417900 4.50 1456858_at Gpr149 BB075339 4.49 1445605_s_at 4921533L14Rik AI835491 4.49 1441048_at 5930430L01Rik BB037822 4.49 1460343_at Neurl AF401228 4.49 1421364_at Lrfn1 NM_030562 4.49 1435666_at Mast3 AW553439 4.48 1434295_at Rasgrp1 BE691356 4.48 1442562_at BB346556 4.48 1418016_at Pum2 BI689507 4.48 1426585_s_at Mapk1 BM209765 4.47 1426450_at Plcl2 BM207017 4.47 1425558_at Klc3 BC017147 4.47 1419838_s_at Plk4 AI385771 4.46 1417178_at Gipc2 NM_016867 4.46 1454069_at 9030409C19Rik AK018495 4.46 1438955_x_at Ppif AV209130 4.46 1458399_at Lrrc3 AI413999 4.45 1448553_at Myh7 NM_080728 4.45 1453782_at 3021401C12Rik BI737125 4.45 1439552_at Trio BE985618 4.44 1450748_at Smpd3 NM_021491 4.44 1422799_at Bat2 AK019427 4.44 1436827_at Gm944 BB053232 4.44 1440359_at 1700012H17Rik BB427277 4.44 1427138_at 0610010D24Rik AW556861 4.44 1454772_at Ascc3l1 AI327392 4.43 1435624_at 9030409G11Rik AV345336 4.42 1423186_at Tiam2 BM228957 4.42 1455548_at Dlgap4 BB314358 4.41 1423973_a_at Arf3 BC024935 4.41 1421979_at Phex U73910 4.40 1452609_at Cox4nb BI133691 4.40 1419757_at Pitpnm2 NM_011256 4.40 1455024_at Tlk1 AV269836 4.40 1439548_at Rap2b BB390705 4.40 1460244_at Upb1 NM_133995 4.40 1435319_at Ihpk2 BB777667 4.39 1451630_at Ttl BC018513 4.39 1440901_at Dgkb BB279524 4.38 1423563_at Prrt1 AA739053 4.38 1426097_a_at Ccdc106 BC018462 4.38 1456755_at Trak1 AI467545 4.38 1423641_s_at Cnot7 BC006021 4.38 1424439_at 1810065E05Rik BC026941 4.38 1429557_at Mcm8 AK010365 4.37 1415999_at Hey1 NM_010423 4.37 1439632_at BQ174397 4.37 1457149_at Ttc22 BB005679 4.36 1423836_at Zfp503 BB447914 4.36 1448378_at Fscn1 NM_007984 4.35 1421897_at Elk1 AI385733 4.35 1437001_at Gsk3b BQ173949 4.35 1435441_at Ablim2 BB336053 4.34 1449110_at Rhob BC018275 4.34 1429122_a_at 1700040I03Rik AK006658 4.34 1450623_at Gnb2 NM_010312 4.33 1427607_at Cacna1h AB041801 4.33 1419924_at Fnip1 AW557298 4.33 1426978_at Klhl2 AW682368 4.32 1421193_a_at Pbx3 NM_016768 4.32 1438722_at 2610014I16Rik AI854368 4.31 1420834_at Vamp2 BG871810 4.31 1425680_a_at Btrc AF110396 4.31 1427930_at Pdxk BG063905 4.31 1418161_at Jph3 NM_020605 4.30 1450863_a_at Dcamkl1 BQ174703 4.30 1439957_at Gnal BF457720 4.29 1447530_at F8a BB274927 4.29 1421260_a_at Srm NM_009272 4.29 1441783_at Ccni BB749856 4.28 1428812_at 1700040L02Rik AK006661 4.28 1452019_at Cyyr1 AF442733 4.28 1454939_at Phf20l1 BB268102 4.28 1420427_a_at Dhx32 NM_133941 4.27 1455632_at Gnb5 BE446953 4.27 1448718_at 2400001E08Rik NM_025605 4.26 1432813_at 2900064F13Rik AK013749 4.26 1430135_at Dnase2a AK018651 4.26 1435845_at BB764479 4.26 1416508_at Med28 AK005130 4.26 1421199_at Dlg2 BB622767 4.26 1420887_a_at Bcl2l1 NM_009743 4.25 1439133_at 4930524O07Rik AV267216 4.25 1417216_at Pim2 NM_138606 4.25 1424037_at Itpka BC027291 4.25 1422683_at Irak1bp1 NM_022986 4.24 1454902_at Prkcz BB430502 4.24 1417233_at Chchd4 NM_133928 4.24 1418181_at pPtp4a3 AK014601 4.24 1424384_a_at Znrf1 BC006765 4.24 1437306_at C130092O11Rik BG071013 4.24 1417890_at Pdxp NM_020271 4.24 1434943_at BC023055 BF464669 4.24 1430358_at Becn1 AV338072 4.23 1438211_s_at Dbp BB550183 4.23 1437780_at Fancb AV222467 4.23 1455865_at Insm1 BB468410 4.22 1456377_x_at LOC632329 AV010467 4.22 1418893_at Pbx2 NM_017463 4.22 1453324_at Nip7 BF459399 4.21 1449063_at Sec22b BC009024 4.21 1428951_at Nol8 AK017551 4.21 1422627_a_at Mkks BF581250 4.21 1433816_at Mcart1 BQ031264 4.21 1439508_at Rab11b BI663913 4.21 1433955_at Brwd1 BB467195 4.20 1436128_at Plekha8 AI324154 4.20 1458457_at BB750877 4.20 1448150_at Nup62 NM_053074 4.20 1460395_at Nudcd3 BB139222 4.19 1434830_at Mxd1 AV228517 4.19 1451884_a_at Lsm2 AF204156 4.19 1447696_x_at Adcy5 BB091427 4.18 1452984_at Ccny AK014507 4.18 1451020_at Gsk3b BB831420 4.18 1448877_at Dlx2 NM_010054 4.17 1455365_at Cdh8 BB426483 4.17 1448498_at Rps6ka4 NM_019924 4.17 1427261_at Wwc1 BQ176786 4.17 1423330_at Ensa AK006149 4.17 1453278_a_at Clip4 AV273445 4.16 1432103_a_at Sh3gl3 AK012114 4.16 1455102_at D330037H05Rik BB213860 4.16 1451917_a_at Dcamkl1 AF155820 4.16 1433657_at A130092J06Rik BI111848 4.15 1442927_at Ptk2b BB548690 4.15 1430037_at Snx27 AK017836 4.15 1449552_at Zfr NM_011767 4.15 1435367_at Mapk4 BQ177154 4.14 1458711_at BB762344 4.14 1428214_at Tomm7 BB609428 4.14 1427673_a_at Sema3e Z93948 4.14 1427450_x_at Myo1b BI080370 4.14 1456290_x_at Ccm2 BB813044 4.13 1432574_at 4930548F15Rik BF466430 4.13 1423897_at Rnf187 AW488376 4.13 1450570_a_at Cd19 NM_009844 4.13 1422977_at Gp1bb NM_010327 4.12 1454844_at Mchr1 AW049955 4.12 1423785_at Egln1 BE995700 4.11 1423012_at Syt7 NM_018801 4.11 1457116_at Rexo1 BI555624 4.10 1416391_at Ptcd1 NM_133735 4.10 1456471_x_at Phgdh BB204486 4.10 1450142_a_at Card14 NM_130886 4.09 1442113_at 5330417C22Rik BB183350 4.09 1439986_at Dgki BE647270 4.09 1448709_at LOC669477 NM_033566 4.09 1423640_at Synpr BC026512 4.08 1458443_at Crtc1 BE986758 4.08 1441894_s_at Grasp BB071890 4.08 1426065_a_at Trib3 BC012955 4.08 1417326_a_at Anapc11 NM_025389 4.08 1434569_at AA474455 BB428484 4.08 1460392_a_at Eny2 BB610210 4.07 1426354_at Bap1 AK009033 4.07 1457704_at Zfp533 BE982894 4.07 1458660_at AI414330 AI414330 4.06 1454721_at 1110018G07Rik AV257687 4.06 1434403_at Spred2 AV229054 4.06 1455271_at LOC620695 BB560177 4.05 1420853_at Sdc3 BB528350 4.05 1431394_a_at Lrrk2 AK014938 4.05 1459983_at Impa2 AI115397 4.05 1455620_at 4930551E15Rik BQ177219 4.04 1454943_a_at Paxip1 BM935413 4.04 1434964_at X83328 BM952039 4.04 1438662_at Ajap1 AW123234 4.03 1452974_at Nol8 AK017551 4.03 1435169_at A930001N09Rik BB431047 4.03 1425271_at Psmc3ip AB000121 4.03 1437642_at Hrbl BB243375 4.03 1419137_at Shank3 NM_021423 4.03 1423673_at Ldoc11 BC023053 4.03 1448360_s_at Angel2 NM_021421 4.03 1424523_at Elmo1 BC024727 4.02 1455206_at C130006E23 BQ175276 4.02 1422527_at H2-DMa NM_010386 4.02 1450928_at Id4 BB121406 4.02 1458351_s_at Klhl2 BB428573 4.01 1422902_s_at Mgea5 NM_023799 4.01 1435942_at Kcnq2 BE993301 4.01 1454960_at Smad3 BI646741 4.00 1433854_at Tmem164 BG064061 4.00 1445653_at BC031361 BB825492 4.00 1442695_at C030007I01Rik AW494399 4.00 1417192_at Tomm70a NM_138599 4.00 1447301_at Akap5 BG228875 4.00 1426416_a_at Yipf4 AV216410 4.00 1438381_x_at Ddx47 BB305306 4.00 1425354_a_at Aggf1 BC027286 4.00 1420192_at D16Bwg1494e N28171 4.00 1458074_at EG328280 BB179240 3.99 1421355_at Tgm3 NM_009374 3.99 1426031_a_at Nfatc2 AF289078 3.99 1446498_at Il20ra BB551879 3.99 1434920_a_at Evl AW553781 3.99 1452531_at Runx1 X97306 3.99 1452541_at Epb4.1l2 AJ245854 3.98 1417375_at Tuba4a AW491660 3.98 1439932_at 3110003A22Rik BQ174624 3.98 1459299_at Myo3b AV381193 3.98 1420590_at Has1 NM_008215 3.98 1448990_a_at Myo1b AI255256 3.98 1454092_a_at Gtf2h3 AK017176 3.97 1435638_at 9130221H12Rik BG808297 3.97 1426580_at Plk4 BB706079 3.97 1423174_a_at Pard6b BE953582 3.97 1450655_at Pten AA214868 3.97 1460214_at Pcp4 NM_008791 3.97 1452281_at Sos2 Z11664 3.97 1429393_at Wdr40a BI558553 3.97 1422897_at Slc22a12 NM_009203 3.96 1416125_at Fkbp5 U16959 3.95 1440213_a_at 2010001M06Rik BE200030 3.95 1420767_at 1700019G17Rik NM_029331 3.95 1423175_s_at Pard6b BE953582 3.95 1435674_at Rhobtb2 BB351953 3.94 1434736_at Hlf BB744589 3.94 1460205_at Dcakd NM_026551 3.94 1422255_at Kcna4 NM_021275 3.94 1433777_at L3mbtl2 BB152370 3.94 1439659_at Usp49 BB014981 3.94 1427161_at Cenpf BE848253 3.94 1448141_at 1110014J01Rik NM_029101 3.94 1418657_at Znhit4 BC019383 3.93 1425006_a_at Vrk1 BC016676 3.93 1436408_at Rprml BE946298 3.93 1453113_at Wdsub1 BB820363 3.93 1415904_at Lpl BC003305 3.92 1426266_s_at Zbtb8os BB417508 3.92 1433860_at 6030458C11Rik BB126127 3.92 1427409_at 8-Mar BB366341 3.92 1436280_at Zfp31 BB757838 3.92 1450853_at Tle4 AU045006 3.91 1460436_at Ndst1 BI652065 3.91 1424434_at BC024814 BC024814 3.91 1435043_at Plcb1 BB794831 3.90 1458413_at Fbxw8 BB357976 3.90 1454675_at Thra BI076689 3.90 1434795_at Disp1 BB174877 3.90 1445854_at C230004F18Rik BB380166 3.90 1420985_at Ash11 BG694892 3.90 1426383_at Cry2 BF303057 3.90 1448467_a_at Ehbp1l1 NM_053252 3.90 1437520_a_at Nup85 BB320388 3.89 1421477_at Cplx2 NM_009946 3.89 1417331_a_at Arl6 NM_019665 3.88 1460738_at Limd2 AK012581 3.88 1417456_at Gnpat NM_010322 3.88 1454789_x_at Prpf6 BB085604 3.88 1452628_at Bag5 AK005534 3.87 1417075_at 2010309E21Rik NM_025591 3.87 1443225_at Acvr1c BB396526 3.87 1438309_at BB432539 3.87 1416744_at Uap1 NM_133806 3.87 1419166_at Slc5a2 BC022226 3.87 1436795_at 9630058J23Rik BM247060 3.86 1448364_at Ccng2 U95826 3.86 1427358_a_at Dapk1 BC026671 3.85 1440505_at Plcxd1 BB187908 3.85 1433847_at D330017J20Rik BB098407 3.85 1417114_at Gmcl1 AF163665 3.85 1419352_at l7Rn6 BC003916 3.84 1428443_a_at Rap1gap AK005063 3.84 1428723_at 2310047M10Rik AK009886 3.84 1426887_at Nudt11 BQ174833 3.84 1422439_a_at Cdk4 NM_009870 3.84 1442039_at Tox BF020502 3.84 1419669_at Prtn3 U97073 3.83 1456970_at Cdh7 BF472513 3.83 1452295_at Tmepai AV291712 3.82 1448511_at Ptprcap NM_016933 3.82 1431646_a_at Stx6 AK009690 3.82 1448414_at Rad1 NM_011232 3.82 1417355_at Peg3 AB003040 3.82 1456441_at Rbm4b BB667164 3.81 1417214_at Rab27b BB121269 3.81 1448554_s_at Myh7 NM_080728 3.81 1453266_at Zbtb4 BB767069 3.81 1419611_at 4632415L05Rik BC023403 3.81 1448919_at Cd302 NM_025422 3.80 1429670_a_at Lrriq2 BB321286 3.80 1421975_a_at Add2 AF189769 3.79 1459054_at BC035954 BB496939 3.79 1458339_at Cdadc1 BB231078 3.79 1427038_at Penk1 M13227 3.79 1417844_at Med4 NM_026119 3.79 1434306_at Rab3ip BF319015 3.78 1439764_s_at Igf2bp2 BB098431 3.78 1435431_at 2310047M15Rik AW558989 3.78 1420397_a_at Spen NM_019763 3.78 1433558_at Dab2ip BM231046 3.78 1420052_x_at Psmb1 C81484 3.78 1439882_at Sec23ip BE685845 3.78 1443952_at Thra BI525006 3.77 1432378_at C030004G16Rik AK021043 3.77 1457897_at Iqce AV245518 3.77 1460671_at Gpx1 BI219063 3.77 1431771_a_at Irak1bp1 AK014712 3.77 1422609_at Arpp19 BE648432 3.77 1427522_at Arhgap20 BB623455 3.76 1426452_a_at Rab30 BG070713 3.76 1452932_at Zbtb7a AK010379 3.76 1441495_at Kctd8 BB183090 3.75 1419225_at Cacna2d3 NM_009785 3.75 1434141_at Gucy1a3 BG072799 3.75 1450662_at Tesk1 NM_011571 3.75 1423217_a_at 2510049I19Rik AV109006 3.75 1434277_a_at Ypel2 BG069663 3.75 1430630_at BB236218 3.74 1436986_at Sntb2 BB219478 3.74 1437006_x_at Becn1 C86082 3.74 1416603_at Rpl22 NM_009079 3.74 1435525_at Kctd17 BI408602 3.74 1437131_x_at Mrpl11 AV045020 3.74 1453428_at 2700045P11Rik AK012380 3.74 1444808_at Pawr BG070555 3.73 1453083_at 6430701C03Rik AK018294 3.73 1435430_at Tmem1 BM200437 3.73 1457310_x_at Scrt2 BG807042 3.73 1434082_at Pctk2 BM243464 3.73 1425920_at Cuedc1 BC025434 3.73 1456106_x_at Sdccag3 BB343189 3.73 1434805_at Mllt1 BG063049 3.73 1422457_s_at Sumo3 NM_019929 3.72 1416113_at Fkbp8 NM_010223 3.72 1417462_at Cap1 NM_007598 3.72 1435825_at Acvrl1 BG969012 3.72 1437703_at EG382156 BM229128 3.71 1428222_at Dcamkl2 AK018179 3.71 1451746_a_at Atg12 AK016474 3.71 1419224_at Cecr6 NM_033567 3.70 1457341_at BE991676 3.70 1428914_at 2310014D11Rik AK009333 3.70 1418081_at Wbscr18 NM_025362 3.70 1426521_at D230025D16Rik BG065702 3.70 1436887_x_at Grwd1 BB251524 3.70 1445970_at Ablim2 BG068300 3.70 1438213_at A830018L16Rik BB128962 3.70 1429591_at Tacc1 BE862546 3.70 1440407_at BE956823 3.69 1426367_at Cab39l AK013205 3.69 1436787_x_at Sec14l3 AV024133 3.69 1448959_at Ndufs4 NM_010887 3.69 1418789_at Sntg2 NM_133742 3.69 1447157_at 4930480K23Rik BB751612 3.68 1444107_at 9430029N19Rik BB091357 3.68 1454815_at Sertad2 BI219769 3.68 1434663_at 2410129H14Rik BI153133 3.68 1434997_at Cdc2l6 BB510904 3.68 1455820_x_at Scarb1 BB138434 3.68 1458027_at Mrpl17 BB343967 3.68 1422787_at Fkbpl NM_019873 3.68 1455562_at Sox12 BF468186 3.68 1421000_at Cnot4 AI448404 3.68 1422495_a_at Hmgn1 NM_008251 3.67 1424731_at Nle1 BC018399 3.67 1451723_at Cnot6l BC018506 3.67 1449353_at Zmat3 NM_009517 3.67 1434825_at Zfp469 BQ175287 3.67 1451291_at Obfc2b BC026942 3.67 1422121_at Oprd1 L06322 3.67 1423116_at Dom3z BG071924 3.67 1429174_at Wdr34 AK014304 3.67 1427286_at D11Bwg0517e BB130195 3.67 1443250_at Rgs2 AV246932 3.66 1440368_at Jmjd2b BG075393 3.66 1435204_at Prmt8 BG065273 3.66 1435270_x_at N6amt2 BF730076 3.66 1427300_at Lhx8 D49658 3.66 1452506_a_at Ilkap BC011427 3.66 1428582_at 2010208K18Rik AK008476 3.66 1430735_at 4930424G05Rik AK015195 3.66 1433907_at Pknox2 BQ175824 3.66 1453485_s_at 1110005A03Rik AK016897 3.65 1437989_at Pde8b BB357157 3.65 1448687_at C1qdc2 NM_026125 3.65 1455703_at Akt2 BG074736 3.65 1426009_a_at Pip5k1b BC003763 3.65 1426701_at 4632419K20Rik BM055600 3.65 1440823_x_at D130058I21Rik BB005209 3.65 1435544_at Exosc6 BB446614 3.65 1455313_at Ablim2 BB336053 3.64 1419216_at Azi1 NM_009734 3.64 1453025_at 2900006F19Rik AK011824 3.64 1423583_at Fem1a AK005041 3.64 1426328_a_at Scn3b AY049036 3.64 1422594_at 5730470L24Rik BC004090 3.64 1426457_at Slmap BB473571 3.64 1437392_at AW123113 AW123113 3.64 1424851_at Chchd5 BE991735 3.63 1445202_at BB526676 3.63 1434653_at Ptk2b AV026976 3.63 1457246_at Rai16 AU040322 3.63 1457864_at Rab11fip3 BB206151 3.63 1419443_at Sap18 NM_009119 3.63 1415761_at Mrpl52 AV021593 3.63 1422710_a_at Cacna1h NM_021415 3.63 1424082_at Tbc1d13 BC025586 3.63 1435128_at Baiap2 BB429313 3.63 1452861_at 2010300C02Rik AK008485 3.62 1449128_at Ccdc43 NM_025918 3.62 1436810_x_at 2900010M23Rik AV046927 3.62 1460261_at Dlg4 AI646416 3.62 1416533_at Egln2 NM_053208 3.62 1433151_at 4933415B22Rik AK016815 3.62 1435921_at D8Ertd587e BM223036 3.61 1421107_at Stk4 NM_021420 3.61 1439569_at Gpr83 AV291031 3.61 1449072_a_at N6amt2 NM_026526 3.61 1439731_at E130309F12Rik BB546288 3.61 1424777_at Wdr21 BC003284 3.61 1438128_at Gnat2 BB427907 3.61 1426894_s_at C230093N12Rik BC023470 3.61 1421739_a_at Matk NM_010768 3.61 1436639_at Bola2 BE951004 3.60 1420059_at 2410166I05Rik AI480750 3.60 1457702_at Gpr12 BB284435 3.60 1451665_a_at Ap4s1 BC001985 3.60 1431056_a_at Lpl AK017272 3.60 1455064_at Rab36 BB313586 3.60 1455958_s_at Pptc7 AI881989 3.60 1438751_at Slc30a10 BB736474 3.60 1429392_at Wdr40a BI558553 3.60 1434216_a_at Nudt19 BG070689 3.59 1439645_at Adra2b BQ031032 3.58 1439557_s_at Ldb2 BB097063 3.58 1429197_s_at Rabgap11 BB431654 3.58 1454219_at Dnajc2 AK013295 3.58 1434704_at Srpk2 BB529352 3.58 1416783_at Tac1 NM_009311 3.58 1429752_x_at Clip4 AV273445 3.58 1422713_a_at Ube2i U31934 3.58 1415762_x_at Mrpl52 AV021593 3.58 1433471_at Tcf7 AI323642 3.58 1416990_at Rxrb BC019432 3.57 1437559_at Rgs7bp BB130891 3.57 1423522_at Npm3 BB811478 3.57 1452452_at M73818 3.57 1435265_at BF466929 3.57 1419285_s_at Cyhr1 NM_019396 3.57 1416285_at Ndufc1 NM_025523 3.57 1452789_at Snn AK012171 3.57 1425211_at 2010003O18Rik BC021775 3.57 1454720_at Hs3st5 AV328620 3.57 1428892_at Ppil1 AK004331 3.57 1439662_at Homer1 BM124609 3.57 1435233_at Ncoa2 BM234716 3.56 1423916_s_at Mlf2 BC003975 3.56 1450560_a_at Ppp2r5d NM_009358 3.56 1430070_at 1500035N22Rik AK005363 3.56 1435472_at Kremen1 BG069617 3.56 1419564_at Zfp467 NM_020589 3.55 1426822_at Rhot2 BM201133 3.55 1436085_at Zbtb34 BB361974 3.55 1442019_at Rcvrn BB627097 3.55 1418135_at Aff1 NM_133919 3.55 1425956_a_at Cdadc1 BC004588 3.55 1416600_a_at Dscr1 AF282255 3.54 1425455_a_at Churc1 BC019472 3.54 1437214_at Lrrtm4 BB667279 3.54 1457097_at Skap2 BB212597 3.54 1433583_at Zfp365 AV327248 3.54 1434567_at 4732496O08Rik BE952228 3.54 1438231_at Foxp2 AV322952 3.54 1456697_x_at Dmtf1 BB248138 3.54 1419173_at Acy1 NM_025371 3.54 1435200_at 6330419J24Rik AV327542 3.53 1447470_at Hdhd2 BF451327 3.53 1440212_at Slc12a1 AI788571 3.53 1436387_at Homer1 BB398124 3.53 1429738_at Myt11 AK013847 3.53 1438379_x_at 2310007F21Rik AV330311 3.53 1436483_at Myt11 BM116113 3.53 1460701_a_at Mrpl52 AV021593 3.53 1425510_at Mark1 BM213279 3.52 1447538_at Crtc1 AW124625 3.52 1415888_at Hdgf NM_008231 3.52 1423161_s_at Spred1 BQ044290 3.52 1448691_at Ubqln4 BB764994 3.52 1424810_at Tasp1 BC005776 3.52 1451751_at Ddit4l AF335325 3.52 1421101_a_at Ldb2 NM_010698 3.52 1460652_at Esrra NM_007953 3.52 1423081_a_at Tomm20 AK002902 3.51 1424191_a_at Tmem41a BC019770 3.51 1425059_at Prmt6 BC022899 3.51 1419754_at Myo5a NM_010864 3.51 1429218_at Det1 BE457100 3.51 1422569_at Yy1 BI665246 3.50 1450844_at Stx6 BQ174465 3.50 1451908_a_at Sec14l1 BC005766 3.50 1448224_at Tfam NM_009360 3.50 1448170_at Siah2 AA414485 3.50 1455885_at Gna12 AV238106 3.50 1455058_at Mtmr9 BM118290 3.50 1460702_at Triap1 AK007514 3.50 1449633_s_at Nt5c31 AI841843 3.50 1420708_at Traip AK012948 3.50 1449472_at Gpr12 NM_008151 3.50 1448889_at Slc38a4 NM_027052 3.49 1420530_at Neud4 AW553317 3.49 1418168_at Zcchc14 BB223737 3.49 1447856_x_at Slc25a25 BB186319 3.49 1449877_s_at Kifc1 NM_016761 3.49 1448947_at 2810004N23Rik NM_025615 3.49 1459372_at Npas4 AV348246 3.48 1422463_a_at Mrpl3 AB049633 3.48 1434693_at BE952823 3.48 1437929_at Dact2 AV306847 3.48 1423461_a_at Ubl3 AV328436 3.48 1454773_at Rxra BQ175050 3.48 1442153_at Uty BB206087 3.48 1419683_at Trp53rk BB400773 3.48 1435141_at Sft2d2 AV238378 3.48 1422487_at Smad4 AK004804 3.48 1421493_a_at Rgs20 NM_021374 3.48 1449392_at Hsd17b1 NM_010475 3.48 1437524_x_at Coro7 BB534801 3.47 1420545_a_at Chn1 NM_029716 3.47 1419445_s_at Sap18 NM_009119 3.47 1426984_at 2310067B10Rik BC007157 3.47 1455609_at C030025P15Rik BB525143 3.46 1418172_at Hebp1 AF117613 3.46 1447959_at Zfp398 AI506402 3.46 1435063_at RP23-25C1.8 AV326964 3.46 1449140_at Nudcd2 BC005646 3.46 1419816_s_at Errfi1 AI788755 3.46 1443129_at BB363699 3.46 1453433_at Wdr89 AK011125 3.46 1418412_at Tpd52l1 NM_009413 3.46 1429417_at 4833446K15Rik AK019528 3.45 1434755_at Coro2b BB538661 3.45 1420374_at Foxj2 BI249549 3.45 1424575_at Rabl5 BC009150 3.45 1433703_s_at Bahd1 BM115038 3.45 1442268_a_at BB749041 3.45 1429573_at Dmrtc1a AK014934 3.45 1448520_at Dclre1b NM_133865 3.45 1455230_at Cacng4 BQ176350 3.45 1451717_s_at Senp2 AV317107 3.44 1436690_at Lrba BB494139 3.44 1415950_a_at Pebp1 NM_018858 3.44 1422486_a_at Smad4 AK004804 3.44 1428526_at Lsmd1 AK018782 3.43 1423038_at Stx6 BQ174465 3.43 1435547_at BQ175722 3.43 1443172_at Orc11 BB210535 3.43 1453262_at 2810032G03Rik AK012851 3.43 1434969_at Brunol5 BB381558 3.43 1450326_at Shc3 NM_009167 3.43 1456786_at Ldb2 BB097063 3.43 1424416_at Nkiras2 BC024398 3.43 1455708_at Tmod3 BB749726 3.43 1425846_a_at Caln1 AF282251 3.42 1418082_at Nmt1 BC016526 3.42 1434183_at LOC666704 BI415031 3.42 1451592_at P42pop AF364868 3.42 1434255_at Pacs2 AV145060 3.42 1424175_at Tef BC017689 3.42 1417626_at Pde4dip NM_031401 3.42 1425479_at Smyd5 BF160651 3.41 1460592_at Epb4.1l1 BI684958 3.41 1424005_at B230219D22Rik BC006718 3.41 1456579_x_at Telo2 BB462079 3.41 1434928_at Gas2l1 BB769886 3.41 1416050_a_at Scarb1 NM_016741 3.40 1420158_s_at Abcf1 AA408356 3.40 1452684_at Akt1s1 BM068933 3.40 1441264_x_at A930005H10Rik AV009179 3.40 1460428_at Ankrd13a BC003286 3.40 1456682_at Lonrf2 AV221843 3.40 1416730_at Rcl1 BC004574 3.40 1418323_at Fem1b BM232562 3.39 1436786_at Sec14l3 AV024133 3.39 1451971_at Cul4a BC024113 3.39 1434085_at Zfp523 AV136654 3.39 1429394_at A130010J15Rik BB025778 3.39 1433526_at Klhl8 BM942651 3.39 1429745_at LOC574405 AK005439 3.39 1436285_at Ccdc113 AV279772 3.39 1433522_at Pskh1 BG073986 3.39 1440286_at Fbxl16 BM232403 3.38 1416977_at Stam2 BB125321 3.38 1447868_x_at Txnl2 BB030680 3.38 1440278_at Dynll1 BM939312 3.38 1448757_at Pml NM_008884 3.38 1434546_at Smg5 BI106637 3.38 1448785_at Runx1t1 BG072085 3.38 1449956_at Prkce NM_011104 3.38 1437037_x_at Snd1 BB240087 3.37 1456606_a_at Phactr1 AV259240 3.37 1457703_at Cacna2d4 BE952838 3.37 1460676_at Josd1 BC006928 3.37 1451046_at Zfpm1 AA014267 3.37 1436525_at Ap3s2 AV225391 3.37 1417710_at Mettl9 NM_021554 3.37 1418250_at Arfl4 NM_025404 3.37 1433680_x_at Siva1 AV216351 3.37 1434922_at Phf12 BB483595 3.37 1424480_s_at Akt2 BC026151 3.37 1427899_at Rnf6 BI738010 3.37 1445685_at Gas7 AI837851 3.36 1454612_at Rkhd2 BI656279 3.36 1452323_at Spryd3 BQ176406 3.36 1421498_a_at 2010204K13Rik NM_023450 3.36 1456427_at Gp1bb BE988990 3.36 1420650_at Atbf1 NM_007496 3.36 1459621_at Itsn1 BB713496 3.36 1453412_a_at Sec14l1 BI652727 3.35 1424195_a_at Inpp5d AF125996 3.35 1450710_at Jarid2 NM_021878 3.35 1430614_at 4632415K11Rik BG962576 3.35 1452015_at 6330416G13Rik AV326978 3.35 1436764_at Pard3 BE199556 3.35 1419470_at Gnb4 BI713933 3.35 1429774_a_at Ccdc98 AK014420 3.35 1434831_a_at Foxo3a BB364488 3.35 1415975_at Carhsp1 AU080787 3.35 1418924_at Rassf7 NM_025886 3.35 1437300_at Mef2d BG067616 3.34 1421175_at Myt11 NM_008666 3.34 1428712_at Mon1b AK017167 3.34 1418080_at B4galt2 AW125856 3.34 1438941_x_at Ampd2 AV330806 3.34 1426679_at Zfp706 AK004076 3.34 1418462_at Exosc9 NM_019393 3.34 1416027_at Pdcd6 NM_011051 3.34 1438313_at BB233366 3.34 1456236_s_at Commd10 AU019438 3.34 1425465_a_at Senp2 AV317107 3.34 1428148_s_at Coro7 BB203098 3.34 1418235_at Atg5 AV168389 3.34 1415749_a_at Rragc NM_017475 3.34 1450539_at Krtap5-1 NM_015808 3.34 1431320_a_at Myo5a AK002362 3.34 1439452_x_at Dnpep BB407163 3.33 1418255_s_at Srf BI662291 3.33 1442093_at Adc AU040368 3.33 1455056_at Lmo7 BM231903 3.33 1427579_at Rhbdl3 BE653576 3.33 1435170_at Tsr2 BQ177187 3.33 1422751_at Tle1 NM_011599 3.32 1459679_s_at Myo1b AA406997 3.32 1447449_at BE983114 3.32 1453183_at 1110034A24Rik BB815668 3.32 1423228_at B4galt6 BG066773 3.32 1423660_at Ctdsp2 BB294133 3.32 1416870_at Dnalc4 NM_017470 3.32 1437597_at BB182944 3.32 1415710_at Cox18 BM123013 3.32 1447831_s_at Mtmr7 BB431693 3.32 1422901_at Mgea5 NM_023799 3.31 1423269_a_at Nedd4l BB663717 3.31 1423350_at Socs5 AA510713 3.31 1451938_a_at Sntb1 BC003748 3.31 1427878_at 0610010O12Rik AK002512 3.31 1436617_at Cetn4 AV211098 3.31 1457790_at Asb3 BB002295 3.31 1441998_at BB369709 3.31 1434706_at Vcpip1 AV175609 3.31 1428950_s_at Nol8 AK017551 3.31 1435974_at Arhgef9 BB271482 3.31 1419650_at Zfr NM_011767 3.30 1443827_x_at BC004044 BB375974 3.30 1460418_x_at H2-T18 X03052 3.30 1426108_s_at Cacnb1 AY094172 3.30 1460021_at EG626231 BB825323 3.30 1432972_at 4921518B13Rik AK014916 3.29 1438727_at Wdr32 BB317629 3.29 1419351_a_at l7Rn6 BC003916 3.29 1429730_at 1810009N02Rik AK007409 3.28 1417311_at Crip2 NM_024223 3.28 1424315_at 1110004E09Rik BC019533 3.28 1448057_at BI320076 3.28 1438932_at Rasgrp2 BE688720 3.28 1455947_at AK162044 BB667208 3.28 1423064_at Dnmt3a BB795491 3.27 1435213_at Nhlrc1 BB309133 3.27 1420377_at St8sia2 BG071333 3.27 1433960_at Isg20l2 BB546077 3.27 1454642_a_at Commd3 BB230296 3.27 1450143_at Rasgrp1 BB354696 3.27 1445668_at Tbce BE993759 3.27 1450106_a_at Evl NM_007965 3.27 1428903_at 3110037I16Rik AK014134 3.27 1437730_at Ppp2r2a C78402 3.27 1417349_at Pldn NM_019788 3.26 1418038_s_at Dusp19 NM_024438 3.26 1424389_at Nupl1 BC026743 3.26 1420870_at Mllt10 BB353133 3.26 1441856_x_at Tysnd1 AV259286 3.26 1448747_at Fbxo32 AF441120 3.26 1424640_at Arl8a BC018479 3.26 1455775_at B230315N10Rik AW988196 3.26 1451106_at Tut1 BC025499 3.26 1435505_at Dmwd BB698273 3.26 1438480_a_at Vps26b AV301675 3.26 1428165_at Vps24 AK009414 3.25 1416126_at Rpo1-2 NM_009086 3.25 1451178_at Mrpl53 BC022162 3.25 1417919_at Ppp1r7 NM_023200 3.25 1446477_at Zfp622 BB038030 3.25 1425081_at Zfp286 BE651907 3.25 1427564_at Diap2 Y15910 3.25 1420643_at Lfng NM_008494 3.25 1425396_a_at Lck BC011474 3.25 1432096_at Snrpn AK010671 3.24 1442524_at AI836848 3.24 1446556_at Kcnd1 BB073575 3.24 1420034_at Ppp2r2d AU019644 3.24 1422083_at Tlr9 AF348140 3.23 1437262_x_at Bcas2 AA940256 3.23 1437861_s_at Prkce BB335101 3.23 1424332_at Rab40c AF422144 3.23 1435604_at Trim37 BM941778 3.23 1435527_at Nfic BB533448 3.23 1454871_at Rbm15b BB776868 3.23 1436138_at Ttc19 BB078627 3.23 1423923_a_at Wdr8 BC016120 3.23 1447249_at BF149076 3.23 1426675_at Tomm70a BB225670 3.23 1435225_s_at Brpf3 BB728354 3.23 1428554_a_at 1810035L17Rik BQ126239 3.22 1437481_at 1110039F03Rik AV032349 3.22 1426398_at Ube2w BB796558 3.22 1440829_x_at Ttll11 AV266869 3.22 1460191_at Ykt6 NM_019661 3.22 1436383_at Cplx2 BE946238 3.22 1421817_at Gsr AK019177 3.22 1438423_at Ssbp2 BB085949 3.21 1452214_at Skil AK018608 3.21 1428672_at Snrpf BI080799 3.21 1440997_at Tnrc6c AV236734 3.21 1454873_at Zfp775 BB667577 3.21 1418986_a_at Uxt NM_013840 3.21 1434058_at Mtmr12 BB667459 3.21 1417044_at Lcmt1 NM_025304 3.21 1435961_at Nat14 BB702376 3.21 1428189_at 5730494M16Rik BQ174627 3.21 1442883_s_at D10Bwg1364e BI413749 3.20 1432478_a_at Ibrdc3 AK015966 3.20 1421968_a_at Nipa2 NM_023647 3.20 1434476_at Crtc1 BM119896 3.20 1433922_at Rab35 BG074549 3.19 1428520_at 1110032A13Rik AK004019 3.19 1419221_a_at Rgs14 NM_016758 3.19 1423317_at 3110001D03Rik BE915283 3.19 1450746_at Keap1 AW764104 3.19 1415792_at Rbck1 NM_019705 3.19 1452958_at Asphd2 AK013495 3.19 1418763_at Nit2 BC020153 3.19 1419081_at Atg10 NM_025770 3.18 1449688_at D10Ertd610e AU014694 3.18 1423873_at Lsm1 BC021460 3.18 1431049_at Ica11 BM964047 3.18 1416501_at Pdpk1 NM_011062 3.18 1438506_s_at Abi1 AV330821 3.18 1434564_at E2f3 BQ176318 3.18 1416432_at Pfkfb3 NM_133232 3.17 1445475_at Pak6 BB003750 3.17 1417461_at Cap1 NM_007598 3.17 1428924_at Mocs3 AU016306 3.17 1422605_at Ppp1r1a NM_021391 3.17 1451680_at Srxn1 BC011325 3.17 1427468_at Ppp3cb M81483 3.17 1455172_at AU020094 BG069639 3.17 1417368_s_at Ndufa2 NM_010885 3.17 1444774_at Det1 BM123155 3.17 1447707_s_at Pde2a BB367174 3.17 1429095_at Cenpp AK006234 3.16 1455289_at Ankrd13b BQ174304 3.16 1431179_at Entpd7 BB873170 3.16 1437715_x_at Apex1 AV100480 3.16 1434243_s_at Tomm70a AV233564 3.15 1424062_at Ube2d1 BC019464 3.15 1435780_at Psd BG966595 3.15 1437363_at Homer1 BQ043238 3.15 1442799_x_at Nudcd3 BB048987 3.15 1455881_at Ier5l BB078200 3.15 1449116_a_at Dtymk NM_023136 3.15 1438217_at A2bp1 AW494804 3.15 1421845_at Golph3 AV174110 3.15 1429929_at Mei1 AK016514 3.15 1429072_at 1110001D15Rik AK003211 3.14 1429715_at Ppp2r2a AK010380 3.14 1421176_at Rasgrp1 BB354696 3.14 1420449_at Heatr5b NM_028084 3.14 1455542_at C630043F03Rik BB226174 3.14 1422441_x_at Cdk4 NM_009870 3.14 1437921_x_at Zfp516 AW744723 3.14 1429108_at Msl211 BB745314 3.14 1423216_a_at 2510049I19Rik AV109006 3.14 1417065_at Egr1 NM_007913 3.14 1449007_at Btg3 NM_009770 3.14 1460352_s_at Pik3r4 BC017537 3.13 1437010_a_at Oaz3 BF319714 3.13 1425599_a_at Gatad1 BC019449 3.13 1424047_at Dera BC016218 3.13 1426861_at Aftph BC004630 3.13 1455771_at Bzrap1 BB467799 3.13 1428824_at 2310003C23Rik AK009106 3.13 1441890_x_at Tmeff1 BB366425 3.13 1436648_at Nanos1 BB251623 3.13 1428267_at Dhx40 AK010512 3.13 1433720_s_at Ndg2 AI647775 3.13 1422526_at Acsl1 BI413218 3.13 1450992_a_at Meis1 AW547821 3.13 1432271_a_at Dcun1d5 AK012764 3.13 1448989_a_at Myo1b AI255256 3.13 1424264_at Med6 BC013096 3.13 1454978_at Ttyh3 BQ180649 3.12 1421500_at Sts NM_009293 3.12 1438416_at Thrap5 BM238407 3.12 1434304_s_at D10Ertd438e BE949414 3.12 1437141_x_at Dym BB549381 3.12 1416622_at Wbscr16 NM_033572 3.12 1452175_at 1810026J23Rik BB027373 3.12 1428926_at 1110003O08Rik AK003388 3.12 1456946_at Sh3md4 BE949337 3.12 1449413_at Mpv17l NM_033564 3.12 1428081_at Klhl21 AK007786 3.12 1447934_at 9630033F20Rik BB128741 3.12 1459998_at Zfp407 AW547438 3.12 1416299_at Shcbp1 NM_011369 3.12 1426247_at Stk24 BG060677 3.12 1422485_at Smad4 AK004804 3.11 1433419_at 4930405A07Rik AK015090 3.11 1459751_s_at Ppp1r16a R75527 3.11 1418575_at Shfm1 NM_009169 3.11 1428327_at Trak1 BB010301 3.11 1428358_at 1810010M01Rik BF581690 3.11 1416743_at Uap1 NM_133806 3.11 1445691_at Chn1 AW121426 3.11 1452593_a_at Tceb1 AI019214 3.11 1439024_at Bag4 BB082119 3.11 1418574_a_at Shfm1 NM_009169 3.11 1451288_s_at 1810043G02Rik BC010330 3.11 1419773_at 2010301N04Rik BB080387 3.11 1416324_s_at Kctd20 AV281062 3.11 1427309_at Pars2 BF583012 3.11 1449634_a_at Anks1b AW046296 3.11 1454032_at Neto2 AK017458 3.11 1419568_at Mapk1 NM_011949 3.10 1418416_x_at Psg23 NM_020261 3.10 1428658_at Pin4 AW012413 3.10 1437743_at Aebp2 BM242614 3.10 1428533_at D1Bwg0212e BG918881 3.10 1455407_at Zfp236 BB282741 3.10 1425781_a_at Plcb1 U85714 3.10 1419984_s_at Zfp644 AU015726 3.10 1435728_at Tyw3 AW457809 3.10 1428474_at Ppp3cb AK004360 3.10 1419995_at D10Ertd641e AU015124 3.10 1435476_a_at Fcgr2b BM224327 3.10 1429382_at Tomm40l AV253376 3.09 1417084_at Eif4ebp2 NM_010124 3.09 1417176_at Csnk1e BE952001 3.09 1457661_at B930036G03Rik BB405928 3.09 1445677_x_at Slc35f2 BB401264 3.09 1439408_a_at Pparbp AV100992 3.09 1427212_at Mapkap1 BC027377 3.09 1455204_at Pitpnc1 BB305507 3.09 1459906_at Dgkh BB655998 3.09 1438306_at Rnf180 AV340072 3.09 1418144_a_at Pip5k1b NM_008847 3.09 1416767_a_at 1110003E01Rik NM_133697 3.09 1428630_x_at Haghl AK021220 3.09 1450685_at Arpp19 BE648432 3.08 1436731_at Zfp533 BB333374 3.08 1438561_x_at Tmem180 BB400326 3.08 1416545_at Zdhhc7 NM_133967 3.08 1436122_at Zfp667 BF467246 3.08 1424317_at Slc25a19 BC018167 3.08 1432447_a_at 2310005N01Rik AK009177 3.08 1458322_x_at E230008N13Rik BB139156 3.08 1416281_at Wdr45l BC004595 3.08 1452026_a_at Pla2g12a AY007382 3.08 1444388_at BB020727 3.08 1428665_at Pfn4 BQ174081 3.08 1416292_at Prdx3 NM_007452 3.08 1416394_at Bag1 NM_009736 3.07 1448611_at Wwc2 NM_133791 3.07 1454996_at Hsdl1 BE985436 3.07 1441356_at Shc2 BB053802 3.07 1437573_at BF018351 3.07 1428593_at 1700029F09Rik BG095162 3.07 1424527_at Ppp2r2d AF366393 3.07 1423519_at 2210412D01Rik BE457744 3.07 1424163_at Rmnd5b BC016075 3.07 1454797_at Tmem55b AW556098 3.06 1448724_at Cish NM_009895 3.06 1437452_x_at Vdac1 AV008054 3.06 1443821_at Lect2 AW122758 3.06 1428585_at Actn1 BE853286 3.06 1426915_at Dapk1 BC021490 3.06 1439611_at Chrm1 BE947522 3.06 1452933_at Ankrd39 AK018681 3.06 1428215_x_at Tomm7 BB609428 3.06 1418656_at Lsm5 NM_025520 3.06 1420390_s_at Zfp354a NM_009329 3.05 1432295_a_at 2310035K24Rik AK009634 3.05 1421123_at Cdk5r1 NM_009871 3.05 1443688_at BC050092 BB710844 3.05 1460372_at Duoxa1 BC019755 3.05 1423967_at Palm BC015297 3.05 1436329_at Egr3 AV346607 3.05 1440343_at Rps6ka5 BQ174267 3.05 1427236_a_at Mll5 BB295149 3.05 1447882_x_at Ddx54 AV318690 3.05 1420219_at 4930461P20Rik BB192718 3.05 1426572_at Me2 BM235734 3.05 1460116_s_at Spred1 AI450584 3.05 1425718_a_at Ivns1abp BC004092 3.04 1447762_x_at Car12 BB503164 3.04 1416664_at Cdc20 NM_023223 3.04 1437679_a_at Glrx2 BB172698 3.04 1459994_x_at Trfr2 AV027486 3.04 1452287_at BB414973 3.04 1435900_at Zbtb43 BF466570 3.04 1415945_at Mcm5 NM_008566 3.04 1424330_at Senp1 BC023129 3.03 1418300_a_at Mknk2 NM_021462 3.03 1418174_at Dbp BC018323 3.03 1420833_at Vamp2 BG871810 3.03 1452046_a_at Ppp1cc BG071790 3.03 1452438_s_at Taf4a BE632382 3.03 1422493_at Cpox BG067254 3.03 1429094_at Ddi2 BI110088 3.03 1427067_at 4933439F18Rik AJ404329 3.03 1426752_at Phf17 BG065238 3.03 1419377_at Med9 BC019367 3.02 1435067_at B230208H17Rik BG075288 3.02 1430305_at 2500004C02Rik AK010870 3.02 1443796_at Il16 BB206877 3.02 1435226_at Ibrdc3 BG064140 3.02 1431352_s_at Pvt1 BI453402 3.02 1428192_at Kbtbd7 AK003597 3.02 1434033_at Tle1 AI325112 3.02 1417840_at 1500031L02Rik NM_025892 3.02 1452275_at Zfand3 BG976649 3.02 1424200_s_at Seh11 AW537349 3.02 1428763_at Smug1 AK004735 3.02 1421395_at Zik1 NM_009577 3.02 1419827_s_at Kif17 AW492270 3.02 1433603_at Ndufs6 C88200 3.02 1431875_a_at E2f1 AK017841 3.02 1453837_at 6330500D04Rik BB867666 3.02 1435714_x_at Il17d BB809808 3.01 1417360_at Mlh1 NM_026810 3.01 1416614_at Eid1 BC010712 3.01 1453348_at 1700085D22Rik AK007007 3.01 1453199_at Acbd6 AK011781 3.01 1431581_at 4922502B01Rik AK013705 3.01 1456183_at Oog4 BB555196 3.01 1421768_a_at Homer1 NM_011982 3.01 1433946_at Zik1 BE824681 3.01 1420849_at Crnkl1 AV143435 3.01 1453274_at Ccdc103 AK006639 3.01 1440005_at Onecut2 BB667396 3.01 1439434_x_at Pcmt1 BB317673 3.01 1460692_at Ehmt2 BI412952 3.01 1422763_at Gipc1 NM_018771 3.01 1455174_at Rps19bp1 AV107461 3.00 1436381_at Dlgap3 BQ175774 3.00 1416991_at Mto1 NM_026658 3.00 1423358_at 1810009K13Rik AK007407 3.00 1430129_a_at Commd8 AK017777 3.00 1448956_at Stard10 NM_019990 3.00 1416439_at 2410015N17Rik NM_023203 3.00 1439869_at 2900054J07Rik BB326106 3.00 1428550_at 1810015A11Rik AK007503 3.00 1439493_at D630040G17Rik BB111712 3.00 1441960_x_at 5730494M16Rik AV267590 2.99 1426465_at Dlgap4 BG066219 2.99 1429438_at Bcor AV318805 2.99 1434848_at BB259283 2.99 1437729_at Rpl27a BG141806 2.99 1457114_at Tor1b BB475906 2.99 1426532_at Zmynd11 BB832996 2.99 1437651_a_at Dtnb AW537479 2.99 1449979_a_at Spock3 NM_023689 2.99 1424790_at Slc25a42 BC025937 2.99 1425848_a_at Dusp26 BC018204 2.99 1452925_a_at 4-Mar AK009364 2.99 1429111_at Tln2 AV270892 2.99 1423285_at Coch BB731671 2.98 1436608_at BC038822 BB457436 2.98 1422762_at Kif17 AB008867 2.98 1443979_at A830021K08Rik AI851680 2.98 1438166_x_at Ndufs4 AV219958 2.98 1439867_at Ubox5 BB079377 2.98 1420885_a_at Sez6 NM_021286 2.98 1416337_at Uqcrb NM_026219 2.98 1425466_at Senp2 AV317107 2.98 1436055_at Lrrc15 AI506528 2.98 1427934_at Lyrm2 AA250510 2.98 1452351_at C030027K23Rik BC025847 2.97 1460703_at Ascc1 AK007519 2.97 1418034_at Mrps9 BC022587 2.97 1448849_at Mrpl40 NM_010922 2.97 1422036_at Strn NM_011500 2.97 1442881_at BF462648 2.97 1417305_at Speg NM_007463 2.97 1460165_at Ppp1ca NM_031868 2.97 1446681_at BB086117 AV327329 2.97 1439838_a_at Tmie BE957021 2.97 1426742_at Atp5f1 AK019459 2.97 1423063_at Dnmt3a BB795491 2.97 1416418_at Gabarapl1 AF180518 2.97 1431304_a_at Tmem183a AU080745 2.97 1448842_at Cdo1 NM_033037 2.97 1454898_s_at Iah1 AU016407 2.97 1455105_at Ptpn12 AV117227 2.96 1452530_a_at Runx1 X97306 2.96 1426640_s_at Trib2 BB354684 2.96 1433761_at Pde4dip AV374669 2.96 1451996_at Tm2d1 AF353993 2.96 1428494_a_at Cox7a1 BB284638 2.96 1456366_at 1700015F17Rik BB833901 2.96 1420103_at AA589431 2.96 1452878_at Prkce AK017901 2.96 1427460_at Taf4a BE632382 2.96 1442408_at 2010305B15Rik BE981170 2.96 1454803_a_at Hdac11 BB183559 2.96 1435865_at Hist3h2a AV297651 2.96 1429918_at Arhgap20 AK018317 2.96 1452607_at 2610030H06Rik BB410125 2.96 1422494_s_at Ccrk NM_053180 2.96 1435260_at Akt3 BG695418 2.95 1438985_x_at Otud5 BB158937 2.95 1434487_at Mef2d AV334508 2.95 1425893_a_at Fhit AF055573 2.95 1429273_at Bmper AK014221 2.95 1427990_at Usp45 BM249286 2.95 1416528_at Sh3bgrl3 NM_080559 2.95 1448027_at 2010305B15Rik AI428254 2.95 1418325_at Sephs2 NM_009266 2.95 1427112_at Ttl AV127581 2.95 1439192_at Nova2 BB524078 2.94 1418434_at Mkrn1 BQ176661 2.94 1458438_at Ccdc122 AW554226 2.94 1449590_a_at Mras AB004879 2.94 1419960_at Anxa11 AU019881 2.94 1456037_x_at Preb BB225012 2.94 1423661_s_at Ctdsp2 BB294133 2.94 1454711_at Trio BB080177 2.94 1441120_at Ceacam16 BB268577 2.94 1449147_at Chst1 NM_023850 2.94 1457719_at AV230618 2.94 1448951_at Tnfrsf1b M60469 2.94 1460434_at Fundc2 AI510221 2.94 1428483_a_at 2610039C10Rik AK012762 2.94 1457513_at 6330569M22Rik BB525203 2.94 1423222_at Cap2 AV261931 2.94 1424514_at Rnf126 BC016543 2.94 1416286_at Rgs4 NM_009062 2.93 1428864_at 5530400B01Rik AK017419 2.93 1441921_x_at Esrrb BB386239 2.93 1424145_at Prr3 BC016636 2.93 1455412_at 9330180L21Rik BG064966 2.93 1426475_at Hmbs AI325144 2.93 1416657_at Akt1 NM_009652 2.93 1449338_at D10Ertd641e BE692118 2.93 1448218_s_at Ywhaz AV027921 2.93 1434365_a_at BC055324 BB093351 2.93 1454759_at Git1 AV230461 2.92 1422976_x_at Ndufa7 NM_023202 2.92 1440555_at Rragd BB354941 2.92 1423551_at Cdh13 BB776961 2.92 1451028_at Baiap2 AA796998 2.92 1436284_s_at Zfp319 BE949045 2.92 1453077_a_at Snapc3 AK016168 2.92 1415794_a_at Spin1 BM228780 2.92 1434345_at Clrn3 BB496366 2.92 1436232_a_at Gabpb1 BQ177003 2.92 1457648_x_at BC004044 BB084182 2.92 1439465_x_at 9430057O19Rik AV277339 2.92 1451037_at Ptpn9 BB224063 2.92 1417032_at Ube2g2 AF296657 2.92 1429623_at Zfp644 AV261187 2.92 1416194_at Cyp4b1 NM_007823 2.92 1445461_at BB096245 2.91 1422414_a_at Calm2 NM_007589 2.91 1450448_at Stc1 BQ032752 2.91 1430046_at Otud3 AK014098 2.91 1451044_at Sip1 AK013414 2.91 1426234_s_at BC002199 BC002199 2.91 1450084_s_at Ivns1abp NM_054102 2.91 1420340_at Cspp1 NM_026493 2.90 1456079_x_at Apex1 AV263745 2.90 1424423_at Lenep BC006806 2.90 1427467_a_at Rpgr AJ238396 2.90 1435422_at 4933433P14Rik AV281088 2.90 1450690_at Ranbp2 BM507707 2.90 1416345_at Timm8a1 W82151 2.90 1429306_at Lzic AK007657 2.90 1428979_at Mtf1 AV316320 2.90 1420107_at Bbs4 C86773 2.90 1437680_x_at Glrx2 BB172698 2.90 1448705_at Zbtb22 BC026964 2.90 1456072_at Ppp1r9a AU067663 2.90 1425600_a_at Plcb1 AW049748 2.90 1455755_at Gm88 BB026554 2.89 1433931_at C030046I01Rik AW553381 2.89 1434169_at 9030409G11Rik BQ175107 2.89 1425777_at Cacnb1 AY094173 2.89 1430803_at Lin28b BB074761 2.89 1421170_a_at Plcb1 BB215274 2.89 1423200_at Ncor1 U22016 2.89 1451409_at 2210021J22Rik BC025858 2.89 1426518_at Tubgcp5 AV333951 2.89 1446768_at BE653578 2.89 1438632_x_at Tnp1 AV206780 2.89 1456582_x_at LOC637796 BB024498 2.89 1425224_at Slmo1 BC019561 2.89 1453380_a_at Xrcc6bp1 AK010471 2.88 1455281_at Wdr33 BB520667 2.88 1415748_a_at Dctn5 NM_021608 2.88 1419690_at 2610002M06Rik NM_025921 2.88 1424785_at Angptl6 BC025904 2.88 1457695_at Ap1gbp1 C76297 2.88 1452051_at Actr3 BE372352 2.88 1425452_s_at AW125753 BC002154 2.88 1449476_at Rage NM_011973 2.88 1426404_a_at Rnf11 BI150320 2.88 1432332_a_at Nudt19 AK008430 2.88 1457378_at Mrpl54 AW123022 2.88 1419144_at Cd163 NM_053094 2.87 1435549_at Trpm4 BI685685 2.87 1449746_s_at Krr1 AU020154 2.87 1441947_x_at BC033915 BB327032 2.87 1451036_at Spg21 BB095517 2.87 1424884_at Fbxw2 BC003834 2.87 1428293_at 2310022M17Rik AK009485 2.87 1460020_at AA930108 W14742 2.87 1419937_at AA536749 C76423 2.87 1426780_at D14Ertd436e AV247381 2.87 1415743_at Hdac5 NM_010412 2.87 1457743_at Rgs7bp BQ175190 2.87 1423399_a_at Yaf2 BG143658 2.87 1440127_a_at AW123182 2.87 1426901_s_at Camta2 BB657856 2.87 1425050_at Isoc1 AK010892 2.87 1417854_at Map2k5 NM_011840 2.86 1451450_at 2010011I20Rik AK008190 2.86 1426850_a_at Map2k6 BB261602 2.86 1420774_a_at 4930583H14Rik NM_026358 2.86 1416574_at 5730589K01Rik NM_023434 2.86 1451722_s_at Smyd5 BF160651 2.86 1447897_x_at Anapc11 AV019615 2.86 1452121_at Fbxo22 BB756840 2.86 1434870_at 2810004N23Rik BB121003 2.86 1441693_at Adamts3 BB193360 2.86 1419763_at 2610020O08Rik NM_025937 2.86 1430208_at Krt42 AK019115 2.86 1456565_s_at Map3k12 BB370469 2.86 1460364_at Gtf2ird1 AI550317 2.86 1458386_at C030009J22Rik BB283503 2.86 1428321_at Eml1 AK003593 2.86 1427449_a_at Adprhl2 BC023177 2.86 1453204_at 4933431K14Rik BB762387 2.86 1419955_at Zfand3 AW539211 2.86 1456964_at Rbm12 BB104271 2.86 1441937_s_at Pink1 AV371921 2.86 1448184_at Fkbp1a AF483488 2.85 1416773_at Wee1 NM_009516 2.85 1416824_at B230118H07Rik NM_026592 2.85 1433762_at C630043F03Rik BB182501 2.85 1433917_x_at D2Bwg1423e BB359828 2.85 1457270_at Rcvrn AI506234 2.85 1429561_at Brf2 AK012680 2.85 1426669_at C530044N13Rik BC025476 2.85 1457610_at Rbck1 BB281404 2.85 1428248_at Nfx1 AK005038 2.85 1432543_a_at Klf13 AK002926 2.85 1429162_at 1500015A07Rik AK005240 2.85 1441892_x_at 4933440H19Rik BB341238 2.85 1416106_at Kti12 NM_029571 2.84 1446937_at Bptf AI450357 2.84 1416928_at Rbm12 NM_029397 2.84 1457061_at Glcci1 BB246045 2.84 1455942_at Fbxl11 BB448023 2.84 1416562_at Gad1 AF326547 2.84 1416128_at Tuba1c NM_009448 2.84 1416586_at Zfp239 NM_008616 2.84 1458107_at BB310489 2.84 1426912_at Rfwd2 BB296692 2.84 1438557_x_at Dnpep BB553721 2.84 1418217_at Nme7 NM_138314 2.84 1453564_a_at Vps24 AK014818 2.84 1441305_at BB360028 2.84 1435523_s_at 2700089E24Rik BB779140 2.84 1439382_x_at Ddr1 BB378700 2.83 1426209_at Strn4 AF414080 2.83 1453064_at Etaa1 AK018594 2.83 1439563_at Zfp3 BB309203 2.83 1418463_at Pik3r2 NM_008841 2.83 1426227_s_at Vps37c BC025865 2.83 1419583_at Cbx4 NM_007625 2.83 1455897_x_at Hmgn1 BF464696 2.83 1448897_at Mkrn2 BI134671 2.83 1439770_at 6430598A04Rik BB387298 2.83 1427296_at BC010304 BB553912 2.83 1451177_at Dnajb4 BC017161 2.83 1440242_at C030014K22Rik BB356428 2.83 1448227_at Grb7 NM_010346 2.83 1438473_at Arl15 BB445175 2.83 1428655_at Ccdc128 AK003781 2.82 1455712_at Hist3h2a AI848909 2.82 1416924_at Bri3 NM_018772 2.82 1440858_at Crkrs BB830220 2.82 1423486_at Cript BB775592 2.82 1427187_at B3gntl1 AV319801 2.82 1417510_at Vps4a NM_126165 2.82 1427909_at 2410015M20Rik BQ031065 2.82 1439106_at Zfp462 AV320128 2.82 1451479_a_at Tmem53 BC019937 2.82 1417448_at 1810008A18Rik NM_133998 2.82 1434326_x_at Coro2b BB317923 2.81 1453846_at Chd2 AK012891 2.81 1434820_s_at Pkig AV209892 2.81 1451073_at Sppl3 BC023131 2.81 1453632_at 4930538K18Rik BB548441 2.81 1460740_at Cltb AK009844 2.81 1426552_a_at Bcl11a BB772866 2.81 1455614_at Tomm40l AV225789 2.81 1434603_at Thrap2 BG074645 2.80 1444106_at D330012F22Rik BB522739 2.80 1435156_at BC046331 BF730275 2.80 1438469_at Bcorl1 BG071122 2.80 1416036_at Fkbp1a AF483488 2.80 1458753_at Gm941 BB548169 2.80 1449125_at Tnfaip8l1 NM_025566 2.80 1453374_at Zfand5 AW744618 2.80 1433450_at Cdk5r1 BB177836 2.80 1447630_x_at Gnb5 BB450318 2.80 1428852_at Dock3 AK018140 2.80 1450557_at Scn4a NM_133199 2.80 1449040_a_at Sephs2 NM_009266 2.80 1454781_x_at Commd9 BB506415 2.80 1417363_at Zfp61 NM_009561 2.80 1451403_at BC024868 BC024868 2.80 1431270_a_at Aste1 AK006153 2.79 1435589_at Ccdc85b AV308222 2.79 1424488_a_at Ppa2 BC011417 2.79 1451098_at Pcoln3 BC010524 2.79 1417918_at Mrpl11 NM_025553 2.79 1450382_at Nf2 L28176 2.79 1448874_a_at Sh2b1 NM_011363 2.79 1458466_at Ctsa AV338634 2.79 1443859_at Rsbn1 AI551821 2.79 1428150_at Coro7 BB203098 2.79 1417265_s_at Coq5 AK014348 2.79 1448845_at Rpp25 NM_133982 2.79 1417657_s_at Dnajc2 BG067003 2.79 1435625_at Entpd7 BG064768 2.79 1427589_at 8430419L09Rik BE283366 2.78 1422770_at Rad51l3 AB052834 2.78 1453821_at N6amt1 BQ033064 2.78 1422510_at Ctdspl NM_133710 2.78 1417207_at Dvl2 BF466091 2.78 1424320_a_at Traf7 BC008598 2.78 1451260_at Aldh1b1 BC020001 2.78 1420199_at Slc35a4 AV144937 2.78 1423304_a_at Rnf111 BB366842 2.78 1451533_at BC022687 BC022687 2.78 1445482_at Gle1l BB375481 2.78 1451518_at Zfp709 BC021921 2.78 1416229_at Rfk NM_019437 2.78 1436086_at 4921526F01Rik AV256234 2.78 1437150_at 1700012H17Rik BB496291 2.78 1459981_s_at Rsbn1 AV240926 2.78 1434481_at 4121402D02Rik BI695596 2.78 1454885_at BC063263 BM211194 2.77 1441534_at C86753 BG067565 2.77 1437267_x_at Hnrph1 BB164501 2.77 1418497_at Fgf13 AF020737 2.77 1438898_at BB309301 2.77 1433858_at Lrrc28 BB667092 2.77 1416093_a_at Mrpl20 NM_025570 2.77 1455185_s_at Phf16 BG064477 2.77 1429053_at 1110012J17Rik BB795266 2.77 1431164_at Rragd AK017818 2.77 1423512_at AW209491 BF715189 2.77 1441317_x_at Jakmip1 BB316060 2.77 1434217_at C330019G07Rik BB400635 2.77 1452119_at 2600005C20Rik BG293527 2.77 1443724_at Jph3 AI849712 2.77 1449947_s_at Atbf1 NM_007496 2.77 1455774_at Jund1 BB049005 2.77 1426818_at Arrdc4 BC025091 2.77 1425053_at Isoc1 AK010892 2.77 1418982_at Cebpa BC011118 2.77 1416448_at Itpa NM_025922 2.77 1415926_at Nup62 NM_053074 2.77 1436808_x_at Mcm5 AI324988 2.76 1420981_a_at Lmo4 NM_010723 2.76 1436902_x_at Tmsb10 BB096368 2.76 1454014_a_at Mkks AK004987 2.76 1417535_at Fbxo25 NM_025785 2.76 1460665_a_at Cnot7 NM_011135 2.76 1455272_at Grm5 BB429139 2.76 1443822_s_at Zcd1 AV347904 2.76 1429779_at Eif2c4 AI481660 2.76 1425114_at Rbbp6 BC025874 2.76 1435097_at Mmab BB772682 2.76 1449511_a_at Ssbp4 NM_133772 2.75 1453305_at Iqcd AK017032 2.75 1420999_at Cnot4 AI448404 2.75 1433780_at Ubn1 BB463209 2.75 1451448_a_at 1110005A03Rik BC026936 2.75 1459920_at 4933415E08Rik AA163908 2.75 1426180_a_at Smr2 U82380 2.75 1436845_at Axin2 BB398993 2.75 1427970_at Zfp689 AV226931 2.75 1427935_at Lyrm2 AA250510 2.75 1453284_at 4930405M20Rik AK015100 2.75 1443890_at BE851943 2.75 1453010_at Iws1 AK006925 2.75 1455673_at Alkbh2 BG070741 2.75 1455169_at Rab11fip2 BG076094 2.75 1419994_s_at D10Ertd641e AU015124 2.75 1452153_at Fbxo18 AF184275 2.75 1415705_at 9130011J15Rik AK018610 2.75 1440205_at Zmynd19 BM195344 2.75 1451223_a_at Btf3l4 AK011367 2.75 1420626_at 2410016F19Rik BF782285 2.74 1459579_at Cacng8 BQ266161 2.74 1426673_at Cdh3 X06340 2.74 1415782_at Sumo2 NM_133354 2.74 1421065_at Jak2 NM_008413 2.74 1421191_s_at Gopc NM_053187 2.74 1439135_at BB129618 2.74 1452271_at Xpr1 AV337591 2.74 1420529_at Neud4 AW553317 2.74 1416768_at 1110003E01Rik NM_133697 2.74 1427879_at 1810031K17Rik AK007667 2.74 1421147_at Terf2 NM_009353 2.74 1452149_at Ube3b AU067784 2.74 1431753_x_at Urm1 AK012124 2.74 1428775_at 1110008L16Rik AK003589 2.73 1433673_at E130309D14Rik BG069691 2.73 1460568_at Trim46 BI156583 2.73 1449109_at Socs2 NM_007706 2.73 1426028_a_at Cit AF086823 2.73 1416805_at 1110032E23Rik NM_133187 2.73 1433710_at Edc3 BM208114 2.73 1420967_at Slc25a15 AW541300 2.73 1459875_x_at 5730494M16Rik AV333371 2.73 1420645_at Pcgf2 NM_009545 2.73 1431939_a_at Mina AK014426 2.73 1453848_s_at Zbed3 AK002774 2.73 1416224_at Zbtb17 NM_009541 2.73 1443719_x_at Ddx42 AW763628 2.73 1453387_at 4833432E10Rik BB095161 2.73 1433992_at Synpo BQ176992 2.73 1434745_at Ccnd2 BQ175880 2.73 1422800_at Bat2 AK019427 2.73 1425478_x_at Ube2i U76416 2.72 1451254_at Ikbkap AF367244 2.72 1419174_at 2410004B18Rik NM_025555 2.72 1443722_at 6030419C18Rik BE945915 2.72 1456098_a_at Elmo2 BG076364 2.72 1425557_x_at Tsc22d3 AF201288 2.72 1419235_s_at Helb BG070273 2.72 1422758_at Chst2 NM_018763 2.72 1448518_at Timm22 AK012130 2.72 1453886_a_at Slc25a26 AK017037 2.72 1426192_at Smarcd2 M60510 2.72 1434630_at Ankrd28 AV261293 2.72 1429908_at 6530403A03Rik AK004216 2.72 1423750_a_at Sf1 BC009091 2.72 1433939_at Aff3 BQ177036 2.72 1426373_at Ski AK019148 2.72 1427071_at Fbxo42 BC003960 2.71 1436561_at Suv39h2 BB440055 2.71 1452179_at Phf17 BG065238 2.71 1440029_at St8sia3 BB379120 2.71 1454800_at Morn2 BF319573 2.71 1421842_a_at Scamp4 AF295102 2.71 1457472_at Tnrc15 BB782031 2.71 1416351_at Map2k1 NM_008927 2.71 1435375_at BC052328 BM224662 2.71 1454650_at Trim35 BQ175280 2.71 1455437_at 9030204A07Rik AV369969 2.71 1427390_at Bloc1s3 BC025913 2.71 1422595_s_at 5730470L24Rik BC004090 2.70 1437801_at Morf41l BM115860 2.70 1417088_at Zfp346 AW493043 2.70 1426316_at 6330416G13Rik AV326978 2.70 1448223_at Fto NM_011936 2.70 1432158_a_at Trappc2 AK007951 2.70 1429238_a_at Ogfod2 AK004916 2.70 1418236_s_at Atg5 AV168389 2.70 1450742_at Bysl BG079188 2.70 1433756_at S100pbp BI452674 2.70 1428609_at Mylc2b AW494458 2.70 1454605_a_at Pi4k2a BB208212 2.70 1448530_at Gmpr NM_025508 2.70 1457635_s_at Nr3c1 BB096079 2.70 1447326_s_at Zmym3 AW122925 2.70 1422725_at Mak BG069426 2.70 1451914_a_at Add2 AF100423 2.70 1419169_at Mapk6 BC024684 2.70 1420634_a_at Smad2 NM_010754 2.69 1423090_x_at Slc37a2 AV216331 2.69 1418865_at Zfp385 NM_013866 2.69 1448195_at Taf5l NM_133966 2.69 1442829_at Rin2 BB128602 2.69 1428695_at 9130227C08Rik BB424872 2.69 1416101_a_at Hist1h1c NM_015786 2.69 1449089_at Nrip1 NM_008735 2.69 1432329_a_at Matk AK018182 2.69 1427299_at Rps6ka3 BE376079 2.69 1433914_at AI747699 BM246193 2.69 1417506_at Gmnn NM_020567 2.69 1426577_a_at 1810054G18Rik BM570079 2.69 1435425_at Pisd AV212090 2.69 1417463_a_at 2400001E08Rik NM_025605 2.69 1440914_s_at Aftph BG071681 2.69 1451379_at Rab22a BC006596 2.69 1418417_at Msc NM_010827 2.69 1424503_at Rab22a BC006596 2.69 1424073_at 5430437P03Rik BC005692 2.68 1448144_at Hnrpab NM_010448 2.68 1434646_s_at Sap18 AV023865 2.68 1447865_x_at Pdzd11 BB037788 2.68 1417959_at Pdlim7 NM_026131 2.68 1441758_at Rhot1 BB830916 2.68 1429931_at 4930553P18Rik AK016112 2.68 1448320_at Stim1 NM_009287 2.68 1418386_at N6amt2 NM_026526 2.68 1459051_at 6530418L21Rik BB781615 2.68 1438765_at Dhx33 BB667230 2.68 1417196_s_at Wwc2 NM_133791 2.68 1420841_at Ptprf BF235516 2.68 1457242_at Ubox5 BB824671 2.68 1447682_x_at Traf5 BB108799 2.68 1436300_at Ripk5 BB435342 2.68 1428354_at Foxk2 BM206907 2.68 1423799_at Eif1 BI693609 2.68 1448637_at Med25 NM_029365 2.68 1460447_at Pus7l AK019372 2.67 1434429_at Syt16 AV348245 2.67 1457717_at Anxa11 AI875033 2.67 1452742_at Trak1 BB010301 2.67 1454767_at D2Bwg1423e BB359828 2.67 1422078_at Akt3 AF124142 2.67 1436758_at Hdac4 AI661423 2.67 1434744_at Yrdc BB230053 2.67 1434624_x_at Rps9 AA762498 2.67 1447302_at BE956835 2.67 1427732_s_at Abcg4 BC026477 2.67 1417778_at Zfp35 NM_011755 2.67 1437539_at Prkaa1 BM236715 2.67 1460572_a_at Zfp511 BQ178976 2.67 1451276_at E030041M21Rik BG066858 2.67 1416781_at Praf2 NM_138602 2.67 1427271_at Zbtb44 BC027138 2.67 1453631_at Stx8 AK011376 2.67 1435618_at Pnma2 BB473446 2.67 1446486_at Dtwd2 BB068132 2.66 1428627_at Zfp509 AK015819 2.66 1457426_at 1700048O20Rik BE980678 2.66 1454835_at Epm2aip1 AV340515 2.66 1428886_at Rbbp5 AK016773 2.66 1460223_a_at Epb4.9 NM_013514 2.66 1436633_at LOC667668 BB323723 2.66 1417215_at Rab27b BB121269 2.66 1418895_at Skap2 NM_018773 2.66 1426972_at Sec24d AK009425 2.66 1459806_x_at Mrps23 BB110309 2.66 1449108_at Fdx1 D43690 2.66 1431076_at Add2 BG976853 2.66 1452846_at Ppfia4 AK003571 2.65 1449913_at Zfp2 NM_009550 2.65 1450184_s_at Tef NM_017376 2.65 1417479_at Ppp2r3c AV367559 2.65 1415952_at Mark2 BI686265 2.65 1450668_s_at Hspe1 NM_008303 2.65 1450436_s_at Dnajb5 AI664344 2.65 1429832_at Ppih AK014665 2.65 1423294_at Mest AW555393 2.65 1442441_at D11Bwg0434e AV008016 2.65 1423302_a_at Paxip1 AW742928 2.65 1434415_at Dact3 BB797871 2.65 1425850_a_at Nek6 BC019524 2.65 1455215_at C530028O21Rik AU067746 2.65 1427870_x_at Igh-6 K00686 2.65 1439776_at Ogfod1 BE956398 2.65 1424474_a_at Camkk2 BI157430 2.65 1423160_at Spred1 BQ044290 2.65 1444502_at Pet112l BG068580 2.64 1460634_at Ralgds NM_009058 2.64 1437927_at Dlg2 AV343743 2.64 1453125_at Sox11 BM508495 2.64 1421619_at Kcnh3 NM_010601 2.64 1451273_x_at BC025546 BC025546 2.64 1427113_s_at Ttl AV127581 2.64 1438201_at Ppm2c AV290622 2.64 1453069_at Pik3cb BF018155 2.64 1455463_at Phyhip BB427286 2.64 1448171_at Siah2 AA414485 2.64 1441727_s_at Zfp467 BE628275 2.64 1428800_a_at Pus7l AK019372 2.64 1423415_at Gpr83 BB110067 2.64 1416514_a_at Fscn1 NM_007984 2.64 1460576_at Exoc6 AV248277 2.64 1426897_at Rcc2 AV122997 2.64 1426307_at Cyb5r4 BC025438 2.63 1450972_at 3110040N11Rik AK019261 2.63 1429278_at Nubpl AK010827 2.63 1444470_x_at Bmp5 AW556834 2.63 1435669_at Zfp532 BB404446 2.63 1426548_a_at Atpbd4 BG070516 2.63 1451334_at 1810009O10Rik BC027022 2.63 1457080_at BB046159 2.63 1456329_at Prtg BB130087 2.63 1417602_at Per2 AF035830 2.63 1447403_a_at Zmynd19 AW125726 2.63 1438922_x_at Slc25a5 AV110784 2.63 1452025_a_at Zfp2 AF483521 2.63 1448630_a_at Sdhc NM_025321 2.63 1456645_at Wdr25 BB352668 2.63 1421820_a_at Nf2 L28176 2.63 1420142_s_at Pa2g4 AA672939 2.63 1455700_at Mterfd3 BB359043 2.63 1434602_at Thrap2 BG074645 2.63 1417016_at Mapkapk5 NM_010765 2.63 1432489_a_at 2410187C16Rik AK010831 2.62 1460498_a_at Dnajc5 BI685030 2.62 1450847_at Ncbp2 BE285362 2.62 1415753_at D10Bwg1364e BC005632 2.62 1437035_x_at Rnf14 BB504639 2.62 1434762_at Tmem142b BF457736 2.62 1451745_a_at Znhit1 BC026751 2.62 1457977_at 2310005N01Rik AI594274 2.62 1440479_at Cbx4 BB821623 2.62 1431836_x_at 5430432N15Rik AK017382 2.62 1441387_at E130309D02Rik BB182811 2.62 1423756_s_at Igfbp4 BC019836 2.62 1427901_at Mrps18c AK004139 2.62 1425656_a_at Baiap2 AF390178 2.62 1435210_s_at Snph BQ174936 2.62 1425304_s_at Prima1 AY043275 2.62 1430127_a_at Ccnd2 AK007904 2.62 1433833_at Fndc3b BG064539 2.62 1452748_at Xrcc3 AW537713 2.62 1419522_at Zmynd19 NM_026021 2.62 1451080_at Usp1 BC018179 2.62 1439405_x_at Becn1 AV296285 2.62 1416247_at Dctn3 NM_016890 2.62 1419987_at C80388 2.62 1416938_at Chchd1 NM_025366 2.62 1426403_at Actr1b BG801851 2.62 1433733_a_at Cry1 BG069864 2.61 1455282_x_at Alas1 AI255644 2.61 1418591_at Dnaja4 NM_021422 2.61 1456785_at Crsp2 BQ175889 2.61 1435844_at A330009N23Rik BB164247 2.61 1441815_at AI851453 AI851453 2.61 1427910_at Cst6 AK003744 2.61 1417507_at Cyb561 BC006732 2.61 1455944_at Zfp516 BB467812 2.61 1451444_s_at 2700008B19Rik BF150550 2.61 1451141_at Mettl8 BC004636 2.61 1427590_at Zfp39 BC011182 2.61 1415925_a_at Nup62 NM_053074 2.61 1433747_at Lnpep BQ176475 2.61 1427966_at BC087945 AW551849 2.61 1448269_a_at Klhl13 NM_026167 2.61 1433593_at Ypel5 BB316456 2.61 1423733_a_at Fiz1 AF126746 2.61 1449339_at D10Ertd641e BE692118 2.60 1452979_at 2610110G12Rik AK014265 2.60 1436102_at Sec22c BB425408 2.60 1428259_at Pxdn AK010185 2.60 1456764_at Slc35f3 BB427860 2.60 1418820_s_at Zcchc10 BC025078 2.60 1448822_at Psmb6 BC013897 2.60 1438481_at AW047464 AI854375 2.60 1435843_x_at Mrps9 AI666552 2.60 1433616_a_at 2310028O11Rik BG793007 2.60 1430128_a_at Reep6 AK002562 2.60 1427898_at Rnf6 BI738010 2.60 1451294_s_at Snrpe BC008262 2.60 1448179_at Usmg5 BC024355 2.60 1460444_at Arrb1 AK004614 2.60 1424358_at Ube2e2 BC016265 2.60 1442060_at Prepl BB224442 2.60 1439630_x_at Sbsn AI844734 2.60 1441610_at N28178 BB306826 2.60 1432232_at Rcor3 AK016486 2.60 1455356_at Camsap1 BG064669 2.59 1429837_at 1700016D18Rik AV278908 2.59 1422616_s_at Wdr54 NM_023790 2.59 1442793_s_at Tbrg4 BB139935 2.59 1456737_x_at Acaa1a BB314154 2.59 1420612_s_at Ptp4a2 BE134116 2.59 1452357_at 4-Sep AF033350 2.59 1460462_at Med18 AK012903 2.59 1429590_at Tacc1 BE862546 2.59 1416796_at Nck2 NM_010879 2.59 1448682_at Dynll1 NM_019682 2.59 1449194_at Mrps25 AK004037 2.59 1429170_a_at Mtf1 AK012676 2.59 1435602_at BE200310 2.59 1422264_s_at Klf9 NM_010638 2.59 1450535_at Krtap12-1 NM_010670 2.59 1455672_s_at Cplx2 BE946238 2.59 1416301_a_at Ebf1 BB125261 2.59 1427173_a_at Mrps33 Y17852 2.59 1456765_at 6430511F03 AV339861 2.59 1449258_at D11Wsu99e AV225714 2.59 1444441_at Rapgefl1 AW490909 2.59 1425147_at 2410075B13Rik BC024536 2.58 1420858_at Pkia AK010212 2.58 1437959_at Zfp324 BB277175 2.58 1443782_x_at Cyp20a1 AV214523 2.58 1451217_a_at Immp1l BC008259 2.58 1443427_at C76966 2.58 1439834_at 2400009B08Rik AA261399 2.58 1460377_a_at Tmem8 BC005722 2.58 1455104_at Mxd1 AV228517 2.58 1452604_at Stard13 BB667840 2.58 1441786_at AW048005 2.58 1436876_at Rgs7bp AV337421 2.58 1449188_at Midn NM_021565 2.58 1435289_at D230014K01Rik BB379430 2.58 1417628_at Supt6h NM_009297 2.58 1417300_at Smpdl3b NM_133888 2.58 1428289_at 2310051E17Rik AW488885 2.58 1428049_a_at Nudt16l1 BC028494 2.58 1422297_at Pfdn5 NM_027044 2.58 1460315_s_at Tbk1 NM_019786 2.58 1420866_at Zfp161 NM_009547 2.57 1449770_x_at D16Bwg1494e N28171 2.57 1455877_a_at Nanos1 BG075296 2.57 1449666_at Atrnl1 AW555641 2.57 1452101_at Blmh BB215260 2.57 1416323_at Kctd20 AV281062 2.57 1435392_at Wdr17 BB277182 2.57 1426487_a_at Rbbp6 BB092954 2.57 1423652_at Isca1 AV209097 2.57 1421557_x_at Txn2 NM_019913 2.57 1453834_at Ssu72 BB857377 2.57 1452988_at 2610306M01Rik AK011997 2.57 1416699_at 1110008F13Rik NM_026124 2.57 1423102_a_at Rnf10 AI746548 2.57 1440478_at D10Ertd438e BM941145 2.57 1427914_a_at Tceb1 AI019214 2.57 1454985_at D030051N19Rik BI872009 2.57 1452516_at Smok3a AJ245454 2.57 1427956_at Pcgf1 BG068910 2.57 1423651_at Isca1 AV209097 2.56 1416155_at Hmgb3 NM_008253 2.56 1460563_at D10Ertd438e BE949414 2.56 1448262_at Psmb2 NM_011970 2.56 1431838_at 1700128E19Rik AK007309 2.56 1437185_s_at Tmsb10 AV148480 2.56 1438422_at Lrrc20 BB143476 2.56 1423456_at Bzw2 BM932775 2.56 1424095_at Rtcd1 BC016519 2.56 1453175_at Zbtb25 AK013374 2.56 1423568_at Psma7 BG297088 2.56 1448505_at C1d NM_020558 2.56 1428619_at 2310005N03Rik BB783243 2.56 1423588_at Arpc4 BG145444 2.55 1421630_at Zfy1 NM_009570 2.55 1427124_at 4932416H05Rik AB041802 2.55 1426810_at Jmjd1a BC026605 2.55 1449076_x_at Adi1 NM_134052 2.55 1427060_at Mapk3 BI414398 2.55 1448882_at Tmem93 NM_025318 2.55 1438689_at 4632433K11Rik BB430846 2.55 1421910_at Tcf20 AW552808 2.55 1426966_at Axin1 BB004060 2.55 1453689_at Fance AK013325 2.55 1452002_at Zfp508 BC024969 2.55 1418121_at Vrk3 NM_133945 2.55 1451595_a_at Kcnq2 AB000497 2.55 1440376_at Fbxo41 AI747062 2.55 1422998_a_at Glrx2 NM_023505 2.55 1426313_at Bre BE984258 2.55 1421342_at Kcns2 NM_008436 2.55 1418732_s_at 1500041N16Rik NM_026399 2.54 1428913_at D2Bwg1335e AK004089 2.54 1428413_at Ccny AK017493 2.54 1422451_at Mrps21 NM_078479 2.54 1436955_at Tssc1 BG065248 2.54 1424240_at Arfip2 BC022942 2.54 1422320_x_at Phxr5 NM_008836 2.54 1454846_at Utp15 AV227804 2.54 1449303_at Sesn3 NM_030261 2.54 1424044_at Jmjd2b BC007145 2.54 1416127_a_at Dnpep NM_016878 2.54 1418112_at Mrpl10 BC016219 2.54 1435019_at Atxn713 BB532141 2.54 1417279_at Itpr1 NM_010585 2.54 1452724_at Ppp1r16a AK013817 2.54 1434228_at Ppm2c AV255921 2.54 1424289_at Osgin2 BB817847 2.54 1416123_at Ccnd2 NM_009829 2.54 1434420_x_at Tomm22 AV102576 2.54 1437148_at Arpc2 BG967632 2.54 1436134_at Scn2b BQ175340 2.54 1439022_at Phactr1 AV259240 2.54 1428603_at Glcci1 AK009885 2.54 1436567_a_at Ndufa7 C88880 2.53 1450484_a_at Tyki AK004595 2.53 1432509_at 5033430I15Rik AK017206 2.53 1431020_a_at Fgfr1op2 AK008983 2.53 1423834_s_at Gga1 BC026802 2.53 1435960_at EG434128 BI733057 2.53 1416507_at Tmem4 NM_019953 2.53 1454987_a_at H2-Ke6 AI323545 2.53 1417774_at Nans BI151886 2.53 1454829_at Rundc1 BB796494 2.53 1452201_at 2310047B19Rik BC019749 2.53 1427006_at Rapgef1 BB339051 2.53 1428528_at 1110007L15Rik BM124553 2.53 1426586_at Slc25a11 AI647500 2.53 1452669_at 2810012G03Rik AK012724 2.53 1452024_a_at Ldb1 AF030333 2.53 1449326_x_at Saa2 NM_011314 2.53 1434381_at BC060631 AV287602 2.53 1428972_at 0610012D17Rik AK007178 2.53 1428515_at 2410012H22Rik AK010472 2.53 1432415_at Rab3c AK014050 2.53 1433460_at Ttc7b BB329157 2.53 1460617_s_at Rab6b AV220161 2.52 1424236_at Tbc1d10b AF285112 2.52 1453737_at Wipf2 BI157060 2.52 1449003_a_at Vti1b NM_016800 2.52 1421992_a_at Igfbp4 NM_010517 2.52 1433682_at Arhgef17 BE287052 2.52 1426716_at Tdrd7 BC025099 2.52 1425650_at Tle4 AF229633 2.52 1455982_at Jmjd4 BF466891 2.52 1449281_at Nrtn NM_008738 2.52 1444531_at Sod2 AI847438 2.52 1426514_at 4631426J05Rik AK019474 2.52 1418827_at Thex1 BF320755 2.52 1422693_a_at Sub1 BE989104 2.52 1457675_at 2510002D24Rik BG063089 2.52 1453030_at Msl2l1 BB745314 2.52 1435912_at Ubxd7 BM220232 2.52 1428447_at Tmem14a AK017734 2.52 1429290_at Cbx6 BE951971 2.52 1451321_a_at Rbm43 BC003333 2.52 1416535_at Mcrs1 BC003746 2.52 1449122_at 3110003A22Rik NM_026534 2.52 1447683_x_at Mettl1 AV118676 2.52 1452048_at Mrpl12 AK002757 2.51 1423502_at Brd2 BI155271 2.51 1428141_at Gga2 AK004632 2.51 1428508_at Tbc1d2b AK004526 2.51 1434832_at Foxo3a BB364488 2.51 1422164_at Pou3f4 X66603 2.51 1451672_at Gprk6 AF040748 2.51 1458979_at BB332816 2.51 1429043_at Smndc1 BG071121 2.51 1428519_at 2610528E23Rik AK019979 2.51 1416850_s_at Zcd1 NM_134007 2.51 1434834_at Socs7 BG067098 2.51 1452380_at Epha7 BB075797 2.51 1420860_at Itga9 NM_133721 2.51 1457250_x_at Nr1d2 BB147330 2.51 1419180_at Bcl9l NM_030256 2.51 1426997_at Thra AW208371 2.51 1431378_at Nr3c1 BB600983 2.51 1451872_a_at Neurl AF400063 2.50 1418915_at Mmachc NM_025962 2.50 1416110_at Slc35a4 NM_026404 2.50 1450061_at Enc1 BM120053 2.50 1448411_at Wfs1 NM_011716 2.50 1422558_at Gamt AF015887 2.50 1441178_at Dtwd2 BB818617 2.50 1424136_a_at Ppih BC016565 2.50 1443133_at Nefm BB394014 2.50 1420472_at Mtpn NM_008098 2.50 1430979_a_at Prdx2 AK011963 2.50 1428531_at Ints7 BB457797 2.50 1427063_at 5330417C22Rik AK017241 2.50 1435902_at Nudt18 BM120193 2.50 1424049_at Lrrc42 BC027203 2.50 1439541_at Mat2a BQ176011 2.50 1455640_a_at Txn2 AV053127 2.50 1444981_at Punc BG067286 2.50 1452921_at B130050I23Rik AK006992 2.50 1437571_at Hic2 BB292220 2.50 1452972_at Ttc32 AK005950 2.50 1415793_at Pnpo NM_134021 2.50 1438387_x_at Top3b BB107606 2.50 1424538_at Ubl4 BI650739 2.50 1426381_at Pprc1 BM199989 2.50 1460307_at Akt3 AF124142 2.50 1416327_at Ufc1 NM_025388 2.49 1423636_at Wdr31 AK017259 2.49 1451140_s_at Prkag2 BB756794 2.49 1426582_at Atf2 BM119623 2.49 1439837_at Tnrc15 BE136147 2.49 1423448_at Rab11b BE986863 2.49 1453020_at 1810048J11Rik AK007829 2.49 1455328_at Accn2 BQ176072 2.49 1452328_s_at Pja2 BF160731 2.49 1419671_a_at Il17rc NM_134159 2.49 1454669_at Tmem11 BG075225 2.49 1438274_at Ikzf4 BG071647 2.49 1420579_s_at Cftr NM_021050 2.49 1448326_a_at Crabp1 NM_013496 2.48 1436994_a_at Hist1h1c BB533903 2.48 1416863_at Abhd8 NM_022419 2.48 1448703_at Lsm8 NM_133939 2.48 1448784_at Taf10 NM_020024 2.48 1434900_at Mkl1 BM196656 2.48 1434843_at A430041B07Rik BG070968 2.48 1419766_at Snf1lk NM_010831 2.48 1416882_at Rgs10 NM_026418 2.48 1436059_at Rfx1 BB151173 2.48 1431387_at AK015329 2.48 1425511_at Mark1 BM213279 2.48 1427496_at Cep152 BC026366 2.48 1455969_at Nudt15 BB198496 2.48 1454018_at Tlk2 AK014829 2.48 1455039_a_at Sin3b BF017589 2.48 1423371_at Pole4 BF577544 2.48 1425884_at Bxdc1 BC025093 2.48 1454874_at Btbd7 BG076293 2.48 1453561_x_at Prkg1 BB809910 2.48 1455489_at Lrrtm2 BB281991 2.48 1430679_at 4933404O12Rik AK016656 2.48 1431334_a_at 4933433P14Rik AK017049 2.48 1434410_at Mina AV276428 2.47 1452343_at D18Ertd653e BQ032363 2.47 1455211_a_at Timm13 BF021416 2.47 1432411_a_at Fbxw2 AK009893 2.47 1437424_at Syde2 BG069296 2.47 1422568_at Ndel1 AF323918 2.47 1428235_at Sdhd AK013962 2.47 1428473_at Ppp3cb AK004360 2.47 1452989_at 2900009J20Rik BB315961 2.47 1419834_x_at Mark1 AW491150 2.47 1442180_at Dleu7 BB382040 2.47 1434029_at 2410025L10Rik AW545676 2.47 1433744_at Lrtm2 BB180412 2.47 1416948_at Mrpl23 NM_011288 2.47 1435636_at 2310051F07Rik AI415636 2.47 1454807_a_at Snx12 BB414983 2.47 1423478_at Prkcb1 BF660388 2.47 1437606_at BB829614 2.46 1434637_x_at Sin3b BF017589 2.46 1435253_at Rab11b BM250605 2.46 1423715_a_at Nedd8 BC004625 2.46 1452467_at Mmab BQ042988 2.46 1436402_at Dohh BG292769 2.46 1427233_at Tshz1 AV291373 2.46 1454851_at Nr2c2 AV162817 2.46 1432091_a_at Rutbc3 AK007455 2.46 1433523_at D930005D10Rik BB521978 2.46 1417230_at Ralgps2 NM_023884 2.46 1440177_at 9.63E+17 BM899529 2.46 1428216_s_at Tomm7 BB609428 2.46 1435675_at Tbc1d12 BF228251 2.46 1416706_at Rpe BG916066 2.46 1439515_at Setd5 BM207556 2.46 1434556_at BI437942 2.46 1448219_a_at Ywhaz AV027921 2.46 1431712_a_at 2310022A10Rik AK009941 2.46 1452039_a_at Bap1 AK009033 2.46 1439371_x_at Timm44 AV102008 2.45 1429033_at Gcc1 AV339946 2.45 1422714_at Ube2i U31934 2.45 1424380_at Vps37b BC026744 2.45 1426888_at Ehmt2 BI412952 2.45 1422418_s_at Supt4h2 NM_011509 2.45 1449080_at Hdac2 NM_008229 2.45 1424940_s_at BC022687 BC022687 2.45 1428457_at 5830472M02Rik AK014262 2.45 1455191_x_at Pip5k1b BB822856 2.45 1424624_at 2900011O08Rik BC022741 2.45 1451187_at 0610037P05Rik BC011300 2.45 1438917_x_at Nup62 AW240611 2.45 1440384_at Nfkbiz AI506779 2.45 1453007_at 3110082I17Rik AK014271 2.45 1423625_a_at Dnajc19 AV053772 2.45 1452791_at Coq2 AK009092 2.45 1422480_at Snx3 NM_017472 2.45 1433924_at Peg3 BM200248 2.45 1417239_at Cetn3 BC002162 2.45 1426112_a_at Cd72 BC003824 2.44 1447540_at Tigd3 AV167924 2.44 1417241_at X83328 NM_025275 2.44 1415686_at Rab14 AV339290 2.44 1448382_at Ehhadh NM_023737 2.44 1422473_at Pde4b BM246564 2.44 1421529_a_at Txnrd1 NM_015762 2.44 1441738_at BE824623 2.44 1424379_at Car11 BC019393 2.44 1434908_at AI480556 BB212188 2.44 1420707_a_at Traip AK012948 2.44 1455279_at Gm1060 BG070552 2.44 1452226_at Rcc2 AV122997 2.44 1428316_a_at Fundc2 AI510221 2.44 1441974_at Camk4 BB209139 2.44 1447653_x_at Rpl24 AV153119 2.44 1452313_at 5930416I19Rik AK011167 2.44 1426769_s_at Maml1 BB522516 2.44 1445479_at Ttbk1 AI839084 2.44 1439180_at A430093A21Rik BE197105 2.44 1431420_s_at Nsd1 BI082843 2.44 1455665_at Lonrf1 BB705689 2.44 1419406_a_at Bcl11a NM_016707 2.44 1420488_at Mrps14 BI246587 2.43 1435747_at Fgf14 AV141013 2.43 1449935_a_at Dnaja3 AK004575 2.43 1451808_at Kcnj4 U11075 2.43 1456767_at Lrfn3 AV062156 2.43 1428797_at Setd6 AK010304 2.43 1437049_at BE627361 2.43 1452860_at Fbxl17 AK004419 2.43 1454805_at Wtap AV141160 2.43 1435039_a_at Pip5k1b BB822856 2.43 1425132_at Neto1 AF448840 2.43 1448543_at Slmo2 NM_025531 2.43 1418234_s_at Bcas2 NM_026602 2.43 1423910_at Centg3 AF459091 2.43 1428097_at 2510009E07Rik AK010940 2.43 1434439_at Gsk3b BQ175580 2.43 1438199_at AI316807 AW228053 2.43 1436763_a_at Klf9 AI267126 2.43 1452833_at Rapgef2 AK018008 2.43 1426012_a_at 2610301G19Rik BC010499 2.43 1429792_at 9530048O09Rik BB398793 2.43 1423843_at Lrrc61 BC027309 2.43 1416853_at Ncdn BC017126 2.43 1438300_at Cdkn2aip BB371618 2.42 1444914_at AW121574 2.42 1429239_a_at Stard4 AK014587 2.42 1440386_at Glce BB272589 2.42 1454640_at Chchd7 BI692256 2.42 1460623_at Skap2 BB753881 2.42 1450942_at Ccdc16 AI530149 2.42 1425052_at Isoc1 AK010892 2.42 1431538_at 4931415C17Rik AV271801 2.42 1428522_at Ttf2 BB283807 2.42 1422938_at Bcl2 NM_009741 2.42 1427039_at Epn1 AF057285 2.42 1424267_at 1810043G02Rik BC010330 2.42 1435755_at 1110001A16Rik BB609468 2.42 1460337_at Sh3kbp1 BB326929 2.42 1452922_at Ppp1r3d AK011539 2.42 1419267_at Nfyb AV250496 2.42 1455327_at Senp2 BI684973 2.42 1453720_at Rnf157 AK011693 2.42 1454695_at Wdr18 BQ176396 2.42 1416135_at Apex1 NM_009687 2.42 1435237_at 2310009A05Rik AV004950 2.42 1455756_at F730014I05Rik BB232864 2.42 1454609_x_at 6430527G18Rik BB770958 2.42 1423971_at Thoc3 BE335845 2.41 1458951_at Vrk1 AV341598 2.41 1436380_at Cdc42bpa BG518726 2.41 1417542_at Rps6ka2 BB737182 2.41 1422718_at Ap3s2 NM_009682 2.41 1455546_s_at Sf3a2 BI900579 2.41 1451170_s_at Nomo1 BC024503 2.41 1429071_at Me3 AK006146 2.41 1426843_at 1810013C15Rik BG065569 2.41 1453013_at Zfp740 AW548228 2.41 1438017_at Rusc1 AV327629 2.41 1457979_at BM938335 2.41 1423189_at 6720456B07Rik BI456243 2.41 1459386_at Zfp709 AV319015 2.41 1422919_at Hrasls NM_013751 2.41 1424293_s_at Tmem55a BC021435 2.41 1455637_x_at EG245305 BB465277 2.41 1424510_at Nudt6 BC027267 2.41 1434853_x_at Mkrn1 AA717142 2.41 1450082_s_at Etv5 BG966751 2.41 1460636_at Map2k2 AW553456 2.40 1428466_at Chd3 AK011183 2.40 1455075_at Pigv BB321057 2.40 1417286_at Ndufa5 NM_026614 2.40 1454963_at E430028B21Rik BQ127172 2.40 1425192_at Klhl25 BC027373 2.40 1430137_at Map3k13 AK004850 2.40 1419189_at Vti1a BC019386 2.40 1435362_at Foxj3 BB026073 2.40 1425071_s_at Ntrk3 BE692701 2.40 1429509_at Lsm12 BB771548 2.40 1455025_at Paqr9 AV103696 2.40 1419138_at B3galt4 NM_019420 2.40 1435556_at Zfp597 AV270881 2.40 1455362_at Angel2 AV275390 2.40 1424520_at 2010305A19Rik BC012391 2.40 1450638_at Pdcd5 AF161074 2.40 1426727_s_at Ppp1r10 BC004771 2.40 1427895_at 2310004N24Rik AK009156 2.40 1452797_at Fastkd3 AK009264 2.40 1423446_at Dapk3 AI642212 2.40 1443948_at Ttc9c BB771921 2.40 1429244_at 1500011B03Rik AW494389 2.40 1449186_at Bag4 NM_026121 2.40 1427413_a_at Cugbp1 AK014492 2.40 1449855_s_at Uchl3 AB033370 2.40 1452254_at Mtmr9 BB292990 2.39 1448483_a_at Ndufb2 NM_026612 2.39 1440364_a_at A230062G08Rik BB760971 2.39 1451347_at Ccdc95 BG065288 2.39 1422798_at Cntnap2 AU079588 2.39 1425180_at Sgip1 AV344708 2.39 1455671_at Commd8 AV365904 2.39 1437239_x_at Phc2 BB334118 2.39 1427158_at Mrps30 AV095945 2.39 1433867_at 1810030O07Rik BG064658 2.39 1451118_a_at 2410018C17Rik BC016596 2.39 1429484_at Kif3c BB752416 2.39 1457279_at BB314393 2.39 1451272_a_at Ube2f BC016117 2.39 1450472_s_at Smad3 BI150236 2.39 1448664_a_at Speg NM_007463 2.39 1434309_at Fntb BG063077 2.39 1416537_at Creld1 NM_133930 2.39 1437848_x_at Adpgk BB490673 2.39 1454637_at Klhl8 BM942651 2.39 1422716_a_at Acp1 AW554436 2.39 1450054_at Add1 BF140063 2.39 1419967_at Seh11 AW540070 2.39 1447320_x_at Rpo1-3 AU018636 2.39 1425940_a_at Ssbp3 AF170906 2.39 1437477_at Lrrfip1 AV301584 2.39 1435802_at ZBTB45 BE134048 2.38 1441453_at Dennd2a BB341122 2.38 1420191_s_at D16Bwg1494e N28171 2.38 1454806_at D12Ertd553e AV228737 2.38 1455497_at Leng9 BB799608 2.38 1418529_at Osgep NM_133676 2.38 1435762_at Pacs1 BM115617 2.38 1424150_at Gdpd5 BC024955 2.38 1417138_s_at Polr2e NM_025554 2.38 1450547_x_at Dub2 U70369 2.38 1424216_a_at Papola U52197 2.38 1453093_at Rasgef1c AK018593 2.38 1416774_at Wee1 NM_009516 2.38 1435598_at BE996371 2.38 1430229_at 6330416L11Rik AK018183 2.38 1436077_a_at Fcho1 BF730694 2.38 1428127_at 4921506J03Rik BG975608 2.38 1423138_at Wdr4 BE854862 2.38 1452873_at 5830415F09Rik AK017931 2.38 1424554_at Ppp1r8 BC025479 2.38 1435683_a_at Abcc5 AV150520 2.38 1423408_a_at 2500003M10Rik BE692070 2.38 1434423_at Gulp1 BB138485 2.38 1443058_at 2900006F19Rik AV242558 2.38 1450943_at 2010012C16Rik AK006303 2.38 1433462_a_at Pi4k2a BB208212 2.37 1417974_at Kpna4 BF018653 2.37 1427326_at 4833439L19Rik BB489450 2.37 1418032_at Itfg2 NM_133927 2.37 1417794_at Zmym3 NM_019831 2.37 1424197_s_at Fance BC016538 2.37 1436053_at Tbc1d22b BB272520 2.37 1418085_at Prkcz NM_008860 2.37 1426344_at Gle11 AK016671 2.37 1460441_at Zxdb BF660409 2.37 1433969_at AU067824 AU067824 2.37 1448582_at Ctnnbl1 NM_025680 2.37 1452657_at Ap1s2 AK005223 2.37 1426811_at Ppp2r5b BB080065 2.37 1448202_x_at 2610524G07Rik NM_025596 2.37 1417339_a_at Dynll1 NM_019682 2.37 1427929_a_at Pdxk BG063905 2.37 1447725_at C030034E14Rik BE948505 2.37 1448542_at Bccip NM_025392 2.37 1435866_s_at Hist3h2a AV297651 2.37 1421196_at Ptpn11 NM_011202 2.36 1455198_a_at Ppp2r3a BB550312 2.36 1418619_at Icam5 NM_008319 2.36 1417612_at Ier5 BF147705 2.36 1417941_at Nanp NM_026086 2.36 1435187_at Tomm20 BG064946 2.36 1440229_at 2310034G01Rik AI854627 2.36 1448817_at Otub1 NM_134150 2.36 1452918_at D19Ertd737e AK011404 2.36 1455032_at Ccnyl1 BQ176303 2.36 1436129_at 4930523C11Rik AV255799 2.36 1428395_at Smurf1 BB832916 2.36 1428991_at Hrasls BQ176967 2.36 1446957_s_at BC004022 C81621 2.36 1460274_at 4921530G04Rik NM_027622 2.36 1424052_at Thap4 BC013538 2.36 1428937_at Atp2b1 BI080417 2.36 1437624_x_at Nudt16l1 AV211435 2.36 1454869_at Wdr40b BB274776 2.35 1451300_a_at Chmp7 BC024115 2.35 1435588_at Wdfy1 BQ031098 2.35 1416267_at Scoc NM_019708 2.35 1441855_x_at Cxcl1 BB554288 2.35 1451726_at Mtmr6 BC020019 2.35 1423984_a_at Mrg2 BC003762 2.35 1423899_at Trip12 BG923744 2.35 1451436_at Sbno1 BC023136 2.35 1418319_at 1810047C23Rik NM_138668 2.35 1423113_a_at Ube2d3 AK009276 2.35 1448946_at Kif3c NM_008445 2.35 1427305_at Piga AV340508 2.35 1438016_at BC068171 BG068512 2.35 1448181_at Klf15 BC013486 2.35 1454944_at Hic2 BE648070 2.35 1435231_at Coq4 BB667890 2.35 1433528_at Gtf2a2 BG075741 2.35 1424710_a_at Gorasp2 BC016455 2.35 1453200_at Rai1 AK013909 2.35 1459657_s_at Rpo1-3 AU018636 2.35 1436523_s_at 1810022K09Rik AV111033 2.35 1431429_a_at Arl4a AK006286 2.35 1454614_at 1810013D10Rik C87702 2.34 1444980_at Onecut2 BE996518 2.34 1457985_at Clmn BE980727 2.34 1456044_at Nedd41 BB309512 2.34 1452208_at Prdm4 AK013393 2.34 1445342_at C230004F18Rik AV338249 2.34 1428742_at Fbxo45 AK011438 2.34 1425830_a_at 2810452K22Rik BC021867 2.34 1422895_at Vamp4 NM_016796 2.34 1437436_s_at Gprk6 BB461269 2.34 1416877_a_at Mrpl51 AI594880 2.34 1416963_at Ubadc1 NM_133835 2.34 1422688_a_at Nras BB018528 2.34 1440228_at Ranbp6 BB477637 2.34 1435685_x_at Abcc5 AV150520 2.34 1420381_a_at Rpl31 NM_053257 2.34 1426620_at Chst10 BB549997 2.34 1428147_at Coro7 BB203098 2.34 1449181_at Fech NM_007998 2.34 1455726_at Gm71 BB756522 2.34 1436081_a_at Zfp414 BE632205 2.34 1452216_at Mcat BB494388 2.34 1425481_at Cnot6l BC018506 2.33 1423032_at Rpl39 AV107150 2.33 1451214_at Kbtbd2 BC022962 2.33 1435883_at Tmco7 BM206080 2.33 1436158_at Eif4ebp2 BI453292 2.33 1456468_x_at 1110012N22Rik BB189996 2.33 1423169_at Taf7 AV213552 2.33 1437336_x_at 1110002E23Rik AV051733 2.33 1429245_at 1810009N02Rik BI202412 2.33 1435426_s_at Pisd AV212090 2.33 1433985_at Abi2 AV263684 2.33 1416502_a_at Preb NM_016703 2.33 1447754_x_at Thap4 BB130418 2.33 1452896_at Gtl3 AK011217 2.33 1428527_at Snx7 AK011015 2.33 1451398_at Cbr4 BC009118 2.33 1416402_at Abcb10 AV382118 2.33 1444661_at Gpr26 BB247791 2.33 1423658_at Sppl3 BC023131 2.33 1455243_at Brpf3 BB728354 2.33 1421262_at Lipg BC020991 2.33 1424771_at H2afj BF661121 2.33 1455430_at Prr8 BQ179705 2.33 1437335_x_at Poldip3 BB534975 2.33 1440124_at Ppap2b BB312387 2.33 1427998_at Lsm12 AV015526 2.33 1434970_a_at AV216669 2.33 1429718_at Slitrk5 BB782729 2.33 1425820_x_at Gpatc4 BC019490 2.33 1438402_at 9630050M13Rik BB131315 2.32 1423794_at D2Ertd391e BM231455 2.32 1423122_at Avpi1 BI649826 2.32 1428718_at Sern1 AW490544 2.32 1439755_at Sipa1l1 BM936013 2.32 1429723_at 6330409N04Rik AK018153 2.32 1452100_at Dullard AV162210 2.32 1417284_at Mapkap1 NM_133841 2.32 1455143_at Nlgn2 AU042744 2.32 1417080_a_at Ecsit NM_012029 2.32 1452680_at Snrpd2 BQ043840 2.32 1427669_a_at Cit AF070066 2.32 1448517_at Timm22 AK012130 2.32 1429759_at Rps6ka6 BB449218 2.32 1448153_at Cox5a NM_007747 2.32 1434794_at Rhof BM241811 2.32 1456557_at 1700041C02Rik BB256666 2.32 1439130_at Slc44a2 BB131502 2.32 1444062_at 2900056L01Rik AI428930 2.32 1441019_at Fbxo3 BB012129 2.32 1450673_at Col9a2 NM_007741 2.32 1436078_at Fcho1 BF730694 2.31 1426786_s_at Dhx38 BM195397 2.31 1448357_at Snrpg NM_026506 2.31 1426408_at Cugbp1 BI412951 2.31 1458040_at D7Wsu130e BM213832 2.31 1440200_at 9630031F12Rik BB128528 2.31 1436373_at Map3k10 AA789425 2.31 1442789_at AW125266 2.31 1418602_at Cdh15 NM_007662 2.31 1458295_at Tmem79 BE626145 2.31 1449172_a_at Lin7b NM_011698 2.31 1433826_at Tspyl3 AV325152 2.31 1453705_at B230110C06Rik AK020961 2.31 1435061_at Nudt10 AI853080 2.31 1449256_a_at Rab11a BC010722 2.31 1452899_at Rian AK017440 2.31 1416952_at Atp6v1d NM_023721 2.31 1459859_x_at Chrac1 BB297047 2.31 1420809_a_at 1500003O03Rik NM_019769 2.31 1429671_at 2410018M08Rik AK010551 2.31 1426222_s_at Loh11cr2a BC004727 2.31 1455208_at Pex19 AV229364 2.31 1455639_at Slc25a39 AV224593 2.31 1431822_a_at Azi2 AK004992 2.31 1451267_at Sharpin BC016203 2.31 1419787_a_at Zfp628 AI608468 2.31 1433971_at Camta1 BB558154 2.31 1424331_at Rab40c AF422144 2.30 1449017_at Nutf2 AA920031 2.30 1452666_a_at Tmcc2 AK004359 2.30 1416018_at Dr1 NM_026106 2.30 1426240_at Chmp4b BC011429 2.30 1437366_at AI608492 BB332542 2.30 1415940_at Zfand2a NM_133349 2.30 1450243_a_at Dscr1l1 NM_030598 2.30 1448968_at D7Wsu128e BC011313 2.30 1431419_at Nsd1 BI082843 2.30 1425507_at Arfrp1 BG695431 2.30 1427427_at Ryr3 AV238793 2.30 1428237_at 2700059D21Rik BI689227 2.30 1442175_at C030027H14Rik BB358264 2.30 1430777_a_at Golph3 AK014644 2.30 1438409_at Cep63 BG061859 2.30 1453169_a_at Gtf2h1 BE688673 2.30 1424296_at Gclc BC019374 2.30 1453570_x_at Bet11 AV252862 2.30 1423823_at Mrto4 BC005734 2.30 1422979_at Suv39h2 NM_022724 2.30 1448131_at Mfn2 NM_133201 2.29 1428524_at Cars2 AK009937 2.29 1417195_at Wwc2 NM_133791 2.29 1428025_s_at Pitpnc1 BC028271 2.29 1423389_at Smad7 BF226166 2.29 1446312_at BB273243 2.29 1424539_at Ubl4 BI650739 2.29 1438434_at Arhgap11a AV349116 2.29 1442763_s_at Ttll10 AW324354 2.29 1418835_at Phlda1 NM_009344 2.29 1416041_at Sgk NM_011361 2.29 1416742_at Cfdp1 NM_011801 2.29 1460439_at BC033915 AK017789 2.29 1438209_at 4632404H12Rik BB216617 2.29 1435799_at Sfrs14 BB772209 2.29 1456789_at Zfp462 AW491540 2.29 1448293_at Ebf1 BB125261 2.29 1423832_at Prkag2 BB756794 2.29 1424620_at D13Wsu177e AK013251 2.29 1422972_s_at Gcn5l2 NM_020004 2.29 1436268_at Ddn AI841578 2.29 1433873_s_at Pcnt BG069597 2.29 1417571_at Mpg BC014754 2.29 1439012_a_at Dck BB030204 2.29 1454012_a_at Parp6 AK021076 2.28 1438091_a_at H2afz AV003424 2.28 1431068_at Rmnd5a BI659732 2.28 1456524_at Nrg1 AV318914 2.28 1440603_at BB073590 2.28 1427279_at Clip4 AK017914 2.28 1442099_at Usp31 BM227490 2.28 1451021_a_at Klf5 BI465857 2.28 1453369_a_at Fundc1 AK007686 2.28 1423833_a_at Brp44 BC018324 2.28 1423534_at Pdcd2 BI526195 2.28 1425929_a_at Rnf14 AF249668 2.28 1435695_a_at A030007L17Rik AA673177 2.28 1421604_a_at Klf3 NM_008453 2.28 1427501_at BC007180 BG083894 2.28 1454034_a_at Usp21 AK013252 2.28 1455023_at N28178 BB171181 2.28 1454749_at Pcnt BG069597 2.28 1456795_at D330027G24Rik BB449568 2.28 1451724_at Ankmy2 BC024959 2.28 1436484_at C030019I05Rik BB800286 2.28 1450910_at Cap2 AV261931 2.28 1434697_at 1110001P04Rik BI692575 2.28 1452800_a_at 0610008C08Rik AK002320 2.28 1460672_at 2410002F23Rik BC016099 2.28 1452812_at Lphn1 AA987131 2.28 1428941_at Zmym2 AK017929 2.28 1435706_at Btbd9 AV232817 2.28 1451348_at Depdc6 BC004774 2.28 1457267_at A130010J15Rik BB667685 2.28 1449048_s_at Rab4a NM_009003 2.28 1416583_at Bad NM_007522 2.28 1417155_at Mycn BC005453 2.28 1443672_at Lars2 AI415729 2.28 1423206_s_at 2310003F16Rik W45949 2.27 1456766_at C330001K17Rik BB036966 2.27 1448711_at Mcm3ap NM_019434 2.27 1459897_a_at Sbsn AI507307 2.27 1428626_at Lysmd2 AK008800 2.27 1424645_at Tnrc6c BB795102 2.27 1424997_at Sfrs8 BC025219 2.27 1439497_at 4933415E08Rik BG065013 2.27 1422647_at Ring1 NM_009066 2.27 1460688_s_at AA407659 BB390422 2.27 1447632_at Fancc BB555719 2.27 1418041_at Tmem186 AW488762 2.27 1437742_at Rab21 BB767504 2.27 1440285_at Ppp1r9a BF472950 2.27 1429874_at Spata17 AV265007 2.27 1454931_at Eid2 BE225694 2.27 1438232_at Foxp2 AV322952 2.27 1429097_at C030044C12Rik BB090042 2.27 1433632_at Irf2bp2 BB183385 2.27 1452521_a_at Plaur X62701 2.27 1417410_s_at Prkci NM_008857 2.27 1455046_a_at Pogz BI250645 2.27 1460739_at Zmiz2 BQ174371 2.27 1432472_a_at Mccc2 AK019760 2.27 1419316_s_at Gnb11 NM_023120 2.27 1434158_at Gmds AI747296 2.27 1415941_s_at Zfand2a NM_133349 2.27 1434214_at 0910001L09Rik BI525016 2.27 1426678_at Zfp706 AK004076 2.27 1419789_at Centg1 AI605509 2.27 1431385_a_at Mbtps1 AK002809 2.27 1460679_at Exosc4 AK005101 2.26 1448875_at Zhx1 NM_009572 2.26 1423211_at Nola3 AK004120 2.26 1436335_at Plch2 BI732150 2.26 1415711_at Arfgef1 BC025221 2.26 1418716_at Mrps25 AK004037 2.26 1434980_at Pik3r5 AV230647 2.26 1431561_a_at Dhx34 AK007461 2.26 1450250_at Otud7a NM_130880 2.26 1433699_at Tnfaip3 BM241351 2.26 1425051_at Isoc1 AK010892 2.26 1434254_at Gna11 BM235739 2.26 1423239_at Impdh1 BB351792 2.26 1433591_at Ppp3r1 BF148071 2.26 1434459_at BC057627 BF147898 2.26 1426430_at Jag2 AV264681 2.26 1451735_at Arfrp1 BG695431 2.26 1420825_at Letm1 BG060855 2.26 1434456_at Gm440 BG075955 2.26 1447899_x_at Tacstd1 AV099587 2.26 1428076_s_at Ndufb4 BG968046 2.26 1460009_at BB667115 2.26 1451016_at Ifrd2 BB540964 2.26 1433572_a_at BC010304 AA673192 2.26 1448915_at Zfp524 NM_025324 2.26 1420978_at Nrf1 NM_010938 2.26 1421903_at Ixl AK005112 2.26 1425462_at Fbxw11 AY038079 2.26 1426710_at Calm3 BB396904 2.26 1449494_at Rab3c AY026947 2.26 1449577_x_at Tpm2 AK003186 2.26 1448720_at Lrrc40 NM_024194 2.26 1429867_at 4933424C08Rik AK016884 2.26 1423107_at Ube2b AK010432 2.25 1429634_at Zfp580 AK005257 2.25 1441773_at 1700061N14Rik AV207074 2.25 1417154_at Slc25a14 NM_011398 2.25 1450466_at B230310J22Rik BI739719 2.25 1458421_at Kcnq3 AW494964 2.25 1417096_at Rrp15 NM_026041 2.25 1437389_x_at AV117555 2.25 1424610_at Trub2 BG064045 2.25 1423335_at 1110004F10Rik AW545354 2.25 1425228_a_at Dguok AY037862 2.25 1417709_at Cyp46a1 NM_010010 2.25 1447757_x_at 1110007A13Rik AV033355 2.25 1426760_at Ipo8 AW413962 2.25 1428285_at 8430427H17Rik AK018446 2.25 1424418_at Slc25a38 BC010801 2.25 1440874_at Slco5a1 BE686667 2.25 1416714_at Irf8 BG069095 2.25 1433902_at Kbtbd8 BB469300 2.25 1429457_at 2310020A21Rik BB001825 2.25 1456585_x_at E130309D02Rik BB546892 2.25 1454680_at D5Ertd579e BG073020 2.25 1422767_at Bys1 BG079188 2.25 1457276_at Snf1lk2 AI315692 2.25 1439934_at Slc30a10 BB540543 2.25 1448358_s_at Snrpg NM_026506 2.25 1453021_at Stxbp5 BM899291 2.25 1423231_at Nrgn AK002933 2.25 1424006_at Aarsd1 BC005711 2.25 1428512_at Bhlhb9 AK012577 2.25 1428266_at Myl3 AK002312 2.25 1426624_a_at Ypel3 BI660196 2.25 1416228_at Pin1 NM_023371 2.24 1434782_at Usp42 AW551594 2.24 1437994_x_at Mier2 BB531179 2.24 1428825_at Nr6a1 AK007201 2.24 1451226_at Pex6 BC003424 2.24 1422579_at Hspe1 NM_008303 2.24 1433621_at Wdr41 BB334979 2.24 1452353_at Gpr155 BB762731 2.24 1417527_at Ap3m2 BC027301 2.24 1429039_s_at 1500034J01Rik BI904336 2.24 1449694_s_at Commd5 C77953 2.24 1427033_at Dnmbp BC025944 2.24 1425264_s_at Mbp BB181247 2.24 1418698_a_at Fech NM_007998 2.24 1428826_at Nr6a1 AK007201 2.24 1452054_at Ube2w BB796558 2.24 1440716_at 6430604M11Rik BB188841 2.24 1424629_at Brca1 U31625 2.24 1435991_at Nr3c2 BQ174990 2.24 1423262_a_at H3f3a BI111967 2.24 1434234_at Zfp341 BM230261 2.24 1434144_s_at 2410187C16Rik BG073505 2.24 1418543_s_at Ccdc43 NM_025918 2.24 1451572_a_at 5230400G24Rik BC016597 2.24 1447739_x_at Klhdc4 AV294746 2.24 1447892_at AV208528 2.24 1453294_at 1700012B15Rik AK007702 2.24 1416885_at 1110038F14Rik NM_054099 2.24 1424419_at Lrrc14 BC022126 2.23 1418899_at Ufm1 NM_026435 2.23 1425624_at Epm2aip1 BC018474 2.23 1429901_at Tcba1 AK018248 2.23 1434116_at Cbx2 BI693188 2.23 1441295_at Lman2l BB712151 2.23 1423945_a_at Pkig BC026550 2.23 1428108_x_at Tmcc2 AK004359 2.23 1457696_at Rilp AI835553 2.23 1436265_at 6330405H19 BB225226 2.23 1419353_at Dpm1 NM_010072 2.23 1424211_at Slc25a33 BC011293 2.23 1433425_at 1700101I19Rik AK007108 2.23 1434509_at Rapgef6 BM250883 2.23 1422589_at Rab3a NM_009001 2.23 1431050_at Rps6ka5 BE291900 2.23 1450204_a_at Mynn AK019238 2.23 1455031_at Cdc2l6 AV278072 2.23 1418545_at Wasf1 NM_031877 2.23 1439113_at LOC77114 BB379753 2.23 1419264_at 1200014M14Rik AK012926 2.23 1460391_at Gtpbp9 AK013055 2.22 1419717_at Sema3e NM_011348 2.22 1424473_at Polr2h BC002306 2.22 1444450_at 9530096D07Rik BB124025 2.22 1415721_a_at 1200013P24Rik AK004750 2.22 1428177_at Trappc6b BG066452 2.22 1459813_at 1700012D01Rik AV040390 2.22 1417557_at Ubxd1 NM_024432 2.22 1448598_at Mmp17 NM_011846 2.22 1458511_at 2410025L10Rik BE687898 2.22 1452069_a_at Thap7 BM210680 2.22 1434139_at Parp11 BB026163 2.22 1455504_a_at Mkrn1 BE133749 2.22 1460733_at AA407659 BB390422 2.22 1429588_at 2810474O19Rik AA509870 2.22 1456480_at Fry BB527078 2.22 1422969_s_at Ihpk1 NM_013785 2.22 1424490_at Zfp428 BC024802 2.22 1418717_at Mrps25 AK004037 2.22 1420196_s_at Tbc1d14 C77429 2.22 1456161_at 0610040B10Rik BE198048 2.22 1429140_at 9830002I17Rik AK020683 2.21 1417517_at Plagl2 NM_018807 2.21 1455286_at Btbd1 AV018484 2.21 1423259_at Id4 BB121406 2.21 1434176_x_at Poldip3 BI963573 2.21 1415936_at Bcar3 NM_013867 2.21 1452817_at Smyd3 AK010447 2.21 1426248_at Stk24 BG060677 2.21 1423378_at Adam23 AI838132 2.21 1460333_at Ddx59 BB829482 2.21 1451078_at 2510039O18Rik BC012878 2.21 1439014_at 1600021P15Rik BB703667 2.21 1428367_at Ndst1 BI652065 2.21 1421297_a_at Cacna1c NM_009781 2.21 1455547_at Zc3h7b BM125518 2.21 1424913_at 2310044G17Rik AK009800 2.21 1451543_at Fbxo21 BC021871 2.21 1434253_s_at Tmcc3 BB711990 2.21 1418327_at 1110058L19Rik NM_026503 2.21 1443773_at Ylpm1 AI851834 2.21 1424300_at Gemin6 BC025157 2.20 1422470_at Bnip3 NM_009760 2.20 1440932_at BB428585 2.20 1425628_a_at Gtf2i AF043220 2.20 1425333_at Rab43 BC010248 2.20 1451166_a_at Ccdc101 BC026784 2.20 1433936_at 0610010E21Rik BG791555 2.20 1455941_s_at Map2k5 BB025010 2.20 1416790_a_at Tdg NM_011561 2.20 1423649_at Tmem68 BC016240 2.20 1453566_at Prr8 AK014289 2.20 1416633_a_at 5730536A07Rik NM_026635 2.20 1423970_at Thoc3 BE335845 2.20 1436976_a_at Pfkfb2 BB201861 2.20 1417387_at Med31 NM_026068 2.20 1428841_at Best1 AK006549 2.20 1455723_at D1Ertd448e AV279416 2.20 1420012_at Xbp1 C77390 2.20 1455273_at BG064695 2.20 1441173_at 4930451G09Rik AV352442 2.20 1436949_a_at LOC668554 AV068352 2.20 1430081_at Phf15 AK004823 2.20 1433878_at Mrps26 AI648866 2.20 1444026_at AI593442 BF658761 2.20 1451624_a_at Phospho2 BC025612 2.20 1418737_at Nudt2 NM_025539 2.20 1450036_at Sgk3 BB768208 2.20 1434315_at Npal3 BB667103 2.20 1435970_at Nlk AU035920 2.20 1458755_at BB125469 2.20 1428468_at 3110043O21Rik AK014175 2.20 1455798_at Galk2 BB771515 2.20 1430021_a_at Sae1 AK011772 2.20 1416910_at Dnajc15 NM_025384 2.20 1418929_at Ift57 NM_028680 2.19 1451075_s_at Ctdsp2 BB294133 2.19 1450520_at Cacng3 NM_019430 2.19 1452839_at Dph5 AK010475 2.19 1449733_s_at Siah1a AA982064 2.19 1416505_at Nr4a1 NM_010444 2.19 1433906_at Asph BE956483 2.19 1428693_at 2610044O15Rik AK011776 2.19 1429367_at Wipi2 BB826276 2.19 1423188_a_at 6720456B07Rik BI456243 2.19 1415729_at Pdpk1 BQ174223 2.19 1417916_a_at Fxc1 NM_019502 2.19 1415746_at Cic AF363690 2.19 1425679_a_at Mapk8ip1 AF109769 2.19 1434196_at Dnaja4 BQ176119 2.19 1416379_at Panx1 NM_019482 2.19 1457699_at E330009J07Rik BB834854 2.19 1428023_at 3110009E18Rik AA879840 2.19 1452777_a_at Nub1 BG066220 2.19 1451188_at Wdr26 BC020044 2.19 1420460_a_at Pex11b NM_011069 2.19 1455588_at Lyrm4 BE952216 2.19 1457990_at Anks1b BB080832 2.19 1459881_at AI595406 AI595406 2.19 1419122_at Mettl1 NM_010792 2.19 1434076_at Wdr37 AV222037 2.19 1424181_at 5-Sep BC010489 2.19 1434427_a_at Rnf157 BB246182 2.19 1460553_at 1700025K23Rik BF466605 2.19 1451568_at A630054L15Rik BC025603 2.18 1428071_at 1110038D17Rik AK004153 2.18 1429042_at 2010200O16Rik AK008415 2.18 1429378_x_at AY761184 AK008498 2.18 1418687_at Arc NM_018790 2.18 1442120_at AA153229 2.18 1439393_x_at Ppp2r4 BB369168 2.18 1448284_a_at Ndufc1 NM_025523 2.18 1450042_at Arx BB322201 2.18 1455234_at B3galt1 BB795733 2.18 1421823_a_at Ppp2cb AI323685 2.18 1434155_a_at 2310061I04Rik BB174350 2.18 1437918_at 4930539E08Rik AV374644 2.18 1460453_at Tagap BM220475 2.18 1428317_at Fundc2 AI510221 2.18 1434995_s_at Dedd BE199280 2.18 1424172_at Hagh BC004749 2.18 1452331_s_at Qser1 BC021511 2.18 1433700_at 4933433P14Rik BB477807 2.18 1436573_at Scrn3 BB434447 2.18 1460320_at Becn1 NM_019584 2.18 1423737_at Ndufs3 BC027270 2.18 1419357_at Isy1 NM_133934 2.17 1431804_a_at Sp3 AK004607 2.17 1451390_s_at Zfand2b BC011495 2.17 1449611_at Cd82 AI894122 2.17 1451005_at Sumo1 BM209585 2.17 1451574_at Bcl9 BC019641 2.17 1453406_a_at Rab28 AK012286 2.17 1426939_at Tsr2 BG070464 2.17 1424250_a_at Arhgef3 BC012262 2.17 1447991_at Pcsk2 AI839700 2.17 1436596_at H2afv BG092470 2.17 1437082_at Akap9 BB246410 2.17 1445194_at Cnksr2 BB355006 2.17 1417903_at Dfna5h NM_018769 2.17 1423663_at Flcn BC025820 2.17 1429213_at 2310030N02Rik AK009547 2.17 1459544_at BB180990 2.17 1456320_at BC049806 BB701297 2.17 1428600_at Nin AK014241 2.17 1419444_at Sap18 NM_009119 2.17 1436405_at Dock4 BG068753 2.17 1455991_at Ccbl2 BG094881 2.17 1436984_at Abi2 BG261647 2.17 1449183_at Comt NM_007744 2.17 1435087_at BC039093 BI409907 2.17 1415688_at Ube2g1 NM_025985 2.16 1434598_at Larp5 BM200204 2.16 1450024_at Sufu AJ308624 2.16 1460188_at Ptpn6 NM_013545 2.16 1452237_at Hrb BB130716 2.16 1446775_at C76272 2.16 1417067_s_at Cabc1 AK014605 2.16 1434266_at AI847670 AI847670 2.16 1415755_a_at Ube2v1 BC019372 2.16 1422119_at Rab5b NM_011229 2.16 1452776_a_at Nub1 BG066220 2.16 1429073_at 2210015D19Rik AK005556 2.16 1443733_x_at Pold3 C85233 2.16 1433544_at Als2cr2 BB277912 2.16 1456089_at Trim23 AV276781 2.16 1418048_at 1110059G10Rik NM_025419 2.16 1424028_at 5830457O10Rik BC023107 2.16 1422516_a_at Fibp NM_021438 2.16 1455003_at AW490810 BQ032496 2.16 1447994_at 1700026B20Rik AV043099 2.16 1429389_at Setmar AK017895 2.16 1429151_at Wdr68 AK011714 2.16 1452923_at Ndufa121 AK007894 2.16 1455042_at Tbl1x BF682509 2.16 1456332_at Tmem17 AV290482 2.16 1455149_at Sh3rf1 BB072090 2.16 1435827_at 4933404O12Rik BB501711 2.15 1439253_x_at 2610524G07Rik AV111335 2.15 1423313_at Pde7a BG070255 2.15 1430361_at 2610005M20Rik BC003771 2.15 1452096_s_at D230025D16Rik BG065702 2.15 1440427_at BB124205 BB127176 2.15 1437617_x_at 1110034G24Rik BB387677 2.15 1429296_at Rab10 BF465974 2.15 1450103_a_at Pscd2 NM_011181 2.15 1455117_at Mcmdc1 AV274182 2.15 1417833_at Zc3h10 NM_134003 2.15 1417873_at Pwp1 BC003199 2.15 1441942_x_at Snupn AV331209 2.15 1438009_at Hist1h2ao W91024 2.15 1424446_at Armc7 BC023126 2.15 1447905_x_at Nup62 BB331755 2.15 1431250_at 9030624G23Rik BG923655 2.15 1428160_at Ndufab1 AK010307 2.15 1453747_at 2810021J22Rik BB620330 2.15 1418726_a_at Tnnt2 NM_011619 2.15 1427163_at Ubr2 AI646734 2.15 1458932_at Pex2 BB639093 2.15 1433639_at 5730593F17Rik AW548096 2.15 1451305_at Pgea1 AF331040 2.15 1431230_a_at Btbd9 Z74630 2.15 1456685_at Nsg2 BB227199 2.15 1422980_a_at Bet11 NM_018742 2.15 1451747_a_at Atg12 AK016474 2.15 1460713_at BC048355 BE949497 2.15 1452584_at 1500032L24Rik BG915677 2.15 1428252_at Chmp2b AA881383 2.15 1427471_at Fbxl3 BF782863 2.15 1436186_at E2f8 BM247465 2.15 1451180_a_at Nt5c3l BC015307 2.15 1420540_a_at Rit1 NM_009069 2.15 1418120_at Rbm8a NM_025875 2.15 1424837_at Rnf113a1 BC024906 2.15 1452888_at 1110034G24Rik AK004090 2.14 1423857_at Mrpl30 BC004614 2.14 1416755_at Dnajb1 AK002290 2.14 1453016_at 2900042B11Rik AK013636 2.14 1415714_a_at 2610209M04Rik BC027564 2.14 1434341_x_at 1110020P15Rik BF681728 2.14 1434250_at Pak2 AW537308 2.14 1426828_at 1300018I17Rik AK005046 2.14 1418314_a_at A2bp1 NM_021477 2.14 1434134_at Wdr42a BB123403 2.14 1455139_at Smarcal1 BB028226 2.14 1432000_a_at Dedd AK006814 2.14 1453048_at Nhlrc2 AK002650 2.14 1459021_at BB307071 2.14 1422677_at Dgat2 AK002443 2.14 1451955_a_at Cacna2d2 AF247140 2.14 1453865_a_at Otud5 AK010750 2.14 1419056_at Rtn2 AF038538 2.14 1433628_at Coq10a BQ044689 2.14 1452601_a_at Acbd6 AK002470 2.14 1434042_s_at Mtmr3 BF466640 2.14 1452938_at Anks1b BQ174247 2.14 1457256_x_at Ptch2 BB530125 2.14 1434104_at Slc35e1 BB041864 2.14 1434218_at C330019G07Rik BB400635 2.14 1452222_at Utrn AI788797 2.14 1449290_at Dpysl5 NM_023047 2.14 1448853_at Synj2bp NM_025292 2.14 1430068_at C030011L09Rik BB356045 2.14 1452207_at Cited2 Y15163 2.14 1437907_a_at Tbca BB559082 2.14 1425710_a_at Homer1 AB019479 2.14 1456218_at Snx22 AV333851 2.14 1419964_s_at Hdgf C80147 2.14 1420008_s_at Wwc1 AU017197 2.14 1428971_at Ccny AK014507 2.14 1425460_at Mtmr2 BB197262 2.14 1426998_at Zfand3 BG976649 2.14 1420852_a_at B3gnt2 AV306734 2.14 1436309_at Neto2 BB125651 2.13 1422859_a_at Rpl23 NM_022891 2.13 1418355_at Nucb2 NM_016773 2.13 1424922_a_at Brd4 BC008532 2.13 1449600_at Gm1683 AA682050 2.13 1427341_at E130103I17Rik U85993 2.13 1423087_a_at 1110002E23Rik BB453951 2.13 1428823_at Hddc2 AK009957 2.13 1440655_at BB076281 2.13 1451265_at Ccdc115 BC019430 2.13 1426496_at Wdr55 AK017695 2.13 1417481_at Ramp1 NM_016894 2.13 1419753_at Nfx1 AK013866 2.13 1424545_at BC003965 W59564 2.13 1457456_at Ttc9b AI481735 2.13 1423240_at Src BG868120 2.13 1423906_at Hsbp1 AK010939 2.13 1459271_at BB036541 2.13 1448196_at Mat2b NM_134017 2.13 1441083_at Clptm1 BB799287 2.13 1439267_x_at Cox5a AV085180 2.13 1437152_at Rkhd3 BG072837 2.13 1418256_at Srf BI662291 2.13 1433855_at Abat BF462185 2.13 1443405_at BB201852 2.13 1454986_at Zfp668 BB152304 2.13 1429869_at 1110020C03Rik AI561792 2.13 1431198_x_at 9430038I01Rik AK020460 2.13 1429380_at Rgs12 AK004813 2.13 1455517_at Rbm4 BB419586 2.13 1425181_at Sgip1 AV344708 2.13 1451570_a_at 6720467C03Rik BC020162 2.13 1450675_at Smap11 NM_133716 2.13 1435197_at Pou3f3 BE993443 2.13 1428989_at 0710001D07Rik AK002941 2.12 1451385_at 2310056P07Rik BC010826 2.12 1427874_at Zfp313 AF502145 2.12 1417105_at Trappc2l NM_021502 2.12 1434092_at Atg9b AW121498 2.12 1434339_at Fnbp11 AW548221 2.12 1433711_s_at Sesn1 BG076140 2.12 1436620_at Ccdc45 BQ173899 2.12 1430832_at 4930426I24Rik BB055339 2.12 1422155_at Hist2h3c2 BC015270 2.12 1419462_s_at Gtl3 NM_008187 2.12 1434951_at Armc8 BE995635 2.12 1451943_a_at Ppm1a AF259673 2.12 1438039_at Hectd1 BE630599 2.12 1416775_at 2310004L02Rik NM_025504 2.12 1417848_at Zfp704 AW413620 2.12 1436449_at BB284627 2.12 1446793_at BB091198 2.12 1449880_s_at Bglap1 NM_007541 2.12 1432431_s_at 2900006F19Rik AK004072 2.12 1438692_at Gtf3c4 BG069995 2.12 1418593_at Taf6 NM_009315 2.12 1440224_at 9330133O14Rik BB077224 2.12 1452140_at Tbc1d20 BC002196 2.12 1434305_at U2af1l4 BF018149 2.12 1415717_at 4931406I20Rik BI735988 2.12 1458350_at AV290089 2.12 1424075_at 9430016H08Rik BC024945 2.12 1459783_s_at Cno AV218165 2.12 1424057_at Gdap2 BC025070 2.12 1454848_at Ppp1r12c BE628628 2.12 1442303_at AV139114 2.12 1425531_at Znhit1 BC026751 2.12 1428757_at Aasdhppt AK013111 2.12 1423635_at Bmp2 AV239587 2.12 1448240_at Mbtps1 NM_019709 2.12 1451290_at Map1lc3a BC010596 2.12 1450926_at Atp6v1g1 BI154058 2.12 1436004_at Usp27x BB021271 2.11 1443836_x_at Wdr48 BB064885 2.11 1456420_at Arid4a BB667227 2.11 1427565_a_at Abcc5 AF213387 2.11 1434934_at Atpaf1 BB771055 2.11 1423675_at Usp1 BC018179 2.11 1434483_at Usp12 AW539189 2.11 1427131_s_at Actb AV234245 2.11 1441764_at Prdm10 BF730739 2.11 1451124_at Sod1 BC002066 2.11 1428405_at Hcfc1r1 BF580567 2.11 1425601_a_at Rtkn BC013820 2.11 1454722_at Pten BG792618 2.11 1424313_a_at Ndufs7 BC013503 2.11 1421315_s_at Cttn BI688355 2.11 1429119_at Iah1 AK005287 2.11 1437350_at RP23-143A14.5 AV290046 2.11 1456109_a_at Mrps15 BB314055 2.11 1451303_at BC002230 BC025577 2.11 1423736_a_at Dym BC018220 2.11 1423245_at Cops7a BG974128 2.11 1428717_at Scrn1 AW490544 2.11 1424529_s_at Cgref1 BC023116 2.11 1433498_at 2010005J08Rik BF585125 2.11 1416668_at Ttc35 NM_025736 2.11 1418632_at Ube2h BI694835 2.11 1428898_at Mon1a AK013387 2.11 1430423_s_at 2610528E23Rik AK019808 2.11 1441203_at BB097040 2.11 1438170_x_at Adrm1 AV166873 2.11 1416920_at Rbm4 NM_009032 2.11 1448963_at Nfyc BC020117 2.11 1419379_x_at Fxyd2 NM_052823 2.11 1455659_at Tmcc1 AA244468 2.11 1443987_at Klhl18 BB283043 2.11 1428106_at 1300001I01Rik AK004841 2.11 1432287_a_at Sntg1 AK016927 2.11 1435566_s_at Araf BG228947 2.10 1433539_at Commd3 BB230296 2.10 1448685_at 2900010M23Rik NM_026063 2.10 1434071_a_at Itga1 AV288264 2.10 1438857_x_at BB058253 2.10 1419803_s_at Ccdc12 C76605 2.10 1440534_at BB177862 2.10 1442994_at Sod2 BQ177195 2.10 1433464_at Ipo13 BB475675 2.10 1451126_at Maf1 BC016260 2.10 1452379_at Auts2 AK012475 2.10 1431087_at Spbc24 BF577722 2.10 1434083_a_at Elmod1 BI738305 2.10 1436990_s_at Ndg2 AA038464 2.10 1448533_at Tbcb NM_025548 2.10 1453364_x_at 9130019O22Rik AK020267 2.10 1438769_a_at Vps26b BF719766 2.10 1424199_at Seh11 AW537349 2.10 1451408_at Trub2 BG064045 2.10 1434435_s_at Cox17 BM941165 2.10 1434019_at Pdap1 BG065186 2.10 1420640_at Jmy BF227962 2.10 1457342_at Ikzf4 BM114149 2.10 1416477_at Ube2d2 NM_019912 2.10 1416550_at Slc35b4 BB320416 2.10 1421051_s_at Vps25 NM_026776 2.10 1424399_at Uck1 BC025146 2.10 1426729_at 2900046G09Rik BC003957 2.10 1420367_at Denr AK010394 2.10 1455959_s_at Gclc AW825835 2.10 1450890_a_at Abi1 AW912678 2.10 1431463_at 1700041C02Rik AK015391 2.10 1443932_at Klhdc1 BQ176961 2.09 1435591_at AI426330 AW495162 2.09 1417609_at Ube2a BG868960 2.09 1423053_at Arf4 BI653265 2.09 1434367_s_at Nutf2 AV217767 2.09 1415799_at Wbp11 NM_021714 2.09 1424013_at Etf1 BC013717 2.09 1449406_at Cyhr1 NM_019396 2.09 1456538_at Sdccag8 BM233019 2.09 1426219_at Scp2 M62361 2.09 1435705_at LOC232875 BB305660 2.09 1423990_at Rab28 BC004580 2.09 1420846_at Mrps2 AV031454 2.09 1418955_at Zfp93 NM_009567 2.09 1451811_at Cacng6 AV091458 2.09 1424784_at 6330416L07Rik AV047635 2.09 1448957_at Rbpsuh NM_009035 2.09 1449099_at Lrba NM_030695 2.09 1426401_at Ppp3ca BQ173889 2.09 1455020_at Snx25 AV351081 2.09 1434438_at Samhd1 BF148012 2.09 1440947_at Bat2d BB481861 2.09 1452690_at Khsrp BQ174458 2.09 1427003_at Ppp2r5c BG072817 2.09 1442999_at B930036G03Rik BB342280 2.09 1453718_at Bcl2l12 AK013411 2.09 1448403_at Lars NM_134137 2.09 1454866_s_at Clic6 BQ176424 2.09 1446624_at Fcmd BB518629 2.09 1423632_at Gpr146 BI103049 2.09 1452721_a_at Ccdc53 BM210204 2.09 1429958_x_at Haghl BI790001 2.09 1438196_at Gpd11 BB727537 2.09 1421524_at Cfc1 NM_007685 2.09 1460363_at Tnrc6c BB795102 2.09 1436522_at Map3k3 BE948629 2.09 1428578_s_at Ppfia4 AK003571 2.08 1455187_at Zbtb40 BM231844 2.08 1460691_at Zfp598 BF385956 2.08 1455507_s_at D8Ertd587e BM223036 2.08 1418996_a_at Lyrm5 BC021522 2.08 1449287_at Srms NM_011481 2.08 1429683_at 5830472M02Rik BG094398 2.08 1426493_a_at Kifc2 BB798224 2.08 1436347_a_at 5530601H04Rik BB501229 2.08 1417211_a_at 1110032A03Rik NM_023483 2.08 1422844_a_at Wdr77 NM_027432 2.08 1452174_at Srebf2 BM123132 2.08 1418568_x_at Srp14 NM_009273 2.08 1419026_at Daxx NM_007829 2.08 1434488_at Arfrp1 BQ176611 2.08 1419912_s_at Strap AW557906 2.08 1438360_x_at Slc25a5 AV003169 2.08 1441578_at Ccdc86 BB142123 2.08 1417825_at Esd NM_016903 2.08 1431059_x_at Htatsf1 AK012522 2.08 1443401_at Astn2 BM941510 2.08 1426643_at Elp3 AK012072 2.08 1455073_at Cdadc1 BB178837 2.08 1420483_at Cnnm3 NM_053186 2.08 1448138_at Ppp2r4 NM_138748 2.08 1428806_at Csnk1g1 AK018639 2.08 1435153_at Btbd6 AV026558 2.08 1431170_at Efna3 AA388313 2.08 1431354_a_at Fars2 AK020164 2.08 1453106_a_at Rnmt AK015403 2.08 1424982_a_at 2700078E11Rik BB137173 2.08 1417591_at Ptges2 NM_133783 2.08 1420611_at Prkacb AV024339 2.08 1417320_at Grpel1 NM_024478 2.08 1451412_a_at Ift20 AY082613 2.08 1438324_at 9330182L06Rik AW550882 2.08 1455583_at Gne AV371374 2.08 1419805_s_at Ggps1 C79210 2.07 1426980_s_at E130012A19Rik BC006054 2.07 1451143_at 1110006G06Rik BC023421 2.07 1416506_at Psma6 NM_011968 2.07 1421066_at Jak2 NM_008413 2.07 1434968_a_at Actr3 BB758476 2.07 1460342_s_at AA536749 U73200 2.07 1457139_at Auts2 AV021813 2.07 1449162_at Pop7 NM_028753 2.07 1423777_at Usp20 AK006800 2.07 1454855_at Magi2 BQ176520 2.07 1428760_at Snapc3 AW537061 2.07 1416776_at Crym NM_016669 2.07 1420888_at Bcl2l1 NM_009743 2.07 1436008_at Tpd52 AV336744 2.07 1433505_a_at Lrrc8d BB315861 2.07 1419454_x_at Pias2 NM_008602 2.07 1423962_at Wdr26 BC020044 2.07 1416834_x_at Ndufb2 NM_026612 2.07 1426017_a_at 0610011L14Rik BC020112 2.07 1426355_a_at 6330578E17Rik BB667858 2.07 1421662_a_at Tusc3 NM_030254 2.07 1421399_at Insm1 NM_016889 2.07 1423303_at Paxip1 AW742928 2.07 1421144_at Rpgrip1 NM_023879 2.07 1437456_x_at Ythdf1 BB332410 2.07 1453342_at Cdc40 AK004569 2.07 1425313_at Carf AF454947 2.07 1421600_a_at Trim26 NM_030698 2.07 1429610_a_at Zfp511 AK010407 2.06 1419630_a_at Trim11 NM_053168 2.06 1431091_at Pygo1 BB595944 2.06 1434994_at Dedd BE199280 2.06 1452914_at 2410024N18Rik AK010587 2.06 1445763_at 1700013F07Rik BG069737 2.06 1428745_a_at 2310003L22Rik AK009123 2.06 1450894_a_at Ap2m1 C76671 2.06 1428681_at 5530400K22Rik AK017426 2.06 1423668_at Zdhhc14 BC021423 2.06 1435092_at Arl4a AV328143 2.06 1457171_at AI854517 BB334885 2.06 1443528_at Ttbk1 BB398560 2.06 1424291_at Nup93 BC023140 2.06 1436034_at Cep68 AW550283 2.06 1429985_at 5430439G13Rik BQ173897 2.06 1452171_at Grwd1 BM200151 2.06 1458541_at Dctn4 BB125218 2.06 1426467_s_at 0610037L13Rik AK002776 2.06 1430769_s_at 2900009I07Rik AW911794 2.06 1452050_at Camk1d BG071931 2.06 1416918_at Dlg3 NM_016747 2.06 1420660_at Lrrc6 NM_019457 2.06 1418975_at Nckipsd NM_030729 2.06 1453795_at Fahd2a BI872590 2.06 1426427_at Ttll1 BB764137 2.06 1443790_x_at 4930414L22Rik AV208084 2.06 1453709_at 3426406O18Rik AV172660 2.06 1418665_at Impa2 NM_053261 2.06 1433707_at Gabra4 BB430205 2.06 1425837_a_at Ccrn4l AF199491 2.06 1435500_at Rab26 BB270778 2.06 1451301_at Tmod2 BB633110 2.06 1456769_at Dusp3 BQ266434 2.06 1443826_x_at Men1 BB269348 2.06 1423460_at Perq1 BI688428 2.06 1439900_at Thtpa BB308405 2.06 1452056_s_at Ppp3ca BQ173889 2.06 1456423_at Ap2m1 AV298746 2.06 1449614_s_at AI314976 AW558560 2.05 1437293_x_at Ust BB561487 2.05 1431985_at 2210010B09Rik AK008693 2.05 1459760_at Ndufs4 BE852624 2.05 1419070_at Cys1 BF466918 2.05 1421945_a_at Bxdc1 NM_023323 2.05 1415678_at Ppm1a BC008595 2.05 1426909_at Uck2 BC004016 2.05 1428345_at Ppapdc2 BB229589 2.05 1448455_at Cln8 AF125307 2.05 1436354_at Dzip11 BE992423 2.05 1460635_at Fastk NM_023229 2.05 1458247_s_at Dctn5 C78178 2.05 1434383_at Pja2 BM114949 2.05 1425410_at Tprkb BC027413 2.05 1442003_at BF537922 2.05 1450706_a_at Arl3 NM_019718 2.05 1436892_at Spred2 BB133520 2.05 1431885_a_at Mus81 AK004647 2.05 1424054_at Btbd2 BC016566 2.05 1420443_at Pcdhb19 NM_053144 2.05 1447926_at Arl5a BB811124 2.05 1434451_at AA216953 2.05 1423440_at 1110001A07Rik AK003196 2.05 1423453_at Nol12 BI408798 2.05 1429383_at Csnk1g3 BM195380 2.05 1455421_x_at 6330503C03Rik AW490145 2.05 1438649_x_at Pebp1 AV207950 2.05 1422885_at Snrpd3 NM_026095 2.05 1430588_at Mro BI076815 2.05 1440459_at Setx BB767941 2.05 1415998_at Vdac1 NM_011694 2.05 1416867_at Bet1 NM_009748 2.05 1417126_a_at Rpl22l1 NM_026517 2.04 1451108_at Rnf185 BC014812 2.04 1434652_at Cdc42bpb BI154551 2.04 1440238_at Gltscr1 BB158599 2.04 1429411_a_at Eny2 AI595744 2.04 1422564_at Actl6b NM_031404 2.04 1441284_at BB283819 2.04 1430017_at 1600016N20Rik AK005476 2.04 1426649_at Tmeff1 BM114154 2.04 1417169_at Usp2 AI553394 2.04 1456602_at 4932417I16Rik AV274211 2.04 1455939_x_at Srp14 AV209126 2.04 1457826_a_at 1810043H04Rik AV056885 2.04 1418646_at Gna-rs1 BF577955 2.04 1434879_at Cdc34 BI794243 2.04 1453132_a_at Gkn2 AK007451 2.04 1416208_at Usp14 BC005571 2.04 1438847_at Mxd3 BB836571 2.04 1416852_a_at Ncdn BC017126 2.04 1434891_at Ptgfrn AV253087 2.04 1418302_at Ppt2 BC013462 2.04 1460381_at BC023179 BC023179 2.04 1429156_at 2610036L11Rik BF453953 2.04 1423976_at 4930453N24Rik BC020029 2.04 1420734_at Ppp1r3f NM_138605 2.04 1422849_a_at Pabpn1 AV028400 2.04 1432003_a_at Rnf41 AK016825 2.04 1422091_at Pfkfb2 BC018418 2.04 1434095_at Unc5a BM226712 2.04 1455118_at D9Ertd402e BM122201 2.04 1419415_a_at Rarg NM_011244 2.04 1449018_at Pfn1 NM_011072 2.04 1436813_x_at Khsrp BB332580 2.04 1449851_at Per1 AF022992 2.04 1427908_at Bnip1 BG073508 2.04 1432435_s_at C030004A17Rik AK012261 2.04 1427025_at Mtmr7 AV094609 2.04 1426256_at Timm17a AF106620 2.04 1424170_at Phf5a BC025161 2.04 1426924_at Rc3h2 AA709668 2.03 1448356_at Ube2d2 NM_019912 2.03 1417847_at Ulk2 NM_013881 2.03 1451442_at Ccdc104 BC005731 2.03 1418966_a_at Dcbld1 AK014521 2.03 1428246_at Vps26b BI081895 2.03 1423757_x_at Igfbp4 BC019836 2.03 1429050_at Chic2 AK015681 2.03 1450386_at Kpna3 BM213828 2.03 1451343_at Vps36 BC010811 2.03 1444208_at Dnahc1 BE950899 2.03 1428075_at Ndufb4 BG968046 2.03 1452635_x_at Josd3 AU044383 2.03 1437937_at Ccbp2 AV220666 2.03 1438412_at Phf17 BM119726 2.03 1441376_at Gabarapl2 BB426412 2.03 1423370_a_at Csnk1g2 AI326320 2.03 1449702_at Zfand2a AU016206 2.03 1457272_at BB284000 2.03 1441782_at Aak1 AW121504 2.03 1442046_at Shroom2 BM119167 2.03 1451736_a_at Map2k7 AW541674 2.03 1460452_at Mospd3 BC003880 2.03 1422018_at Hivep2 NM_010437 2.03 1452626_a_at 1810014F10Rik AK007494 2.03 1435072_at Zfyve1 AV327165 2.03 1416748_a_at Mre11a NM_018736 2.03 1418117_at Ndufs4 NM_010887 2.03 1433897_at AI597468 BQ176744 2.03 1447689_at Gprasp1 AV221773 2.03 1451664_x_at Klra7 AF283248 2.03 1460209_at Usp39 BC026983 2.03 1455841_s_at Grwd1 BB251524 2.03 1435645_at Mmd AA472735 2.03 1453569_s_at Mark4 AK010751 2.03 1427426_at Kcnq5 AV362204 2.03 1426692_at Ccdc97 AK004762 2.02 1452324_at Pvt1 BE956863 2.02 1453002_at Sox11 BE825056 2.02 1421520_at Jph1 NM_020604 2.02 1430500_s_at Mtx2 AK005233 2.02 1449711_at Atp6v1e1 C85064 2.02 1420969_at Btbd14b NM_025788 2.02 1428756_at Aasdhppt AK013111 2.02 1418384_at Apool BC024334 2.02 1460697_s_at 2610209M04Rik BC027564 2.02 1434165_at Clic6 BQ176424 2.02 1433970_at Bola3 BB610351 2.02 1416240_at Psmb7 NM_011187 2.02 1450908_at Eif4e BB406487 2.02 1416192_at Napa BC004804 2.02 1435105_at 1110061N23Rik BG066986 2.02 1456149_at D11Bwg0517e BB386621 2.02 1456125_a_at EG627788 AV006937 2.02 1427702_at Zfp1 X16493 2.02 1443875_at Gm603 BG075083 2.02 1428498_at 2610206B13Rik AK011896 2.02 1454236_a_at C030004A17Rik AK012261 2.02 1434009_at Grlf1 BQ176422 2.02 1448762_at Rad17 NM_011233 2.02 1457778_at Stox2 BB364290 2.02 1450080_at Cxx1c NM_028375 2.02 1431510_s_at 2010110K16Rik AK008567 2.02 1448247_at Bcl7b NM_009745 2.02 1426763_at Oaz2 AW214584 2.02 1418905_at Nubp1 NM_011955 2.02 1439553_s_at Nutf2 AU018817 2.02 1437242_at Ttl112 AV121345 2.02 1452753_at Foxk2 BM206907 2.02 1423146_at Hes5 AV337579 2.02 1456032_x_at H2afz AV215230 2.02 1435886_at Dnajb12 AW456220 2.02 1456013_x_at Slc35a4 BB396486 2.01 1428234_at Cpsf6 BB425379 2.01 1428689_at Tysnd1 BF466063 2.01 1454769_at Tatdn2 BB314680 2.01 1422750_a_at Zmynd10 NM_053253 2.01 1435432_at Centg2 BE688580 2.01 1429799_at Zfp606 BB541617 2.01 1428513_at Calcoco1 AK007393 2.01 1416698_a_at Cks1b NM_016904 2.01 1416373_at Nfs1 NM_010911 2.01 1460562_at Eftud1 BB042938 2.01 1445221_at BB451452 2.01 1430675_at 2900055J20Rik BM932606 2.01 1419736_a_at Eiflay NM_025437 2.01 1423738_at Oxa11 BC027191 2.01 1459846_x_at Tmem4 AV100225 2.01 1425621_at Trim35 AB060155 2.01 1451207_at Cbara1 BC023022 2.01 1429796_at Kalrn AK008844 2.01 1430292_a_at 1810030N24Rik BG792484 2.01 1426665_at Katnb1 AK010364 2.01 1455303_at Rfxap BB735651 2.01 1426613_a_at Snrpb2 AV066554 2.01 1422560_at Rsc1a1 NM_023544 2.01 1437985_a_at 2310061I04Rik BB379386 2.01 1421891_at St3gal2 BB750118 2.01 1430569_at Ttc9c AK008753 2.01 1427915_s_at Tceb1 AI019214 2.01 1425780_a_at Tmem167 BC024352 2.01 1450394_at Golph3 AV174110 2.01 1416499_a_at Dctn6 NM_011722 2.01 1434249_s_at Trim9 BB083438 2.01 1430030_at 5330426P16Rik AK019912 2.01 1423479_at Nol11 AK005123 2.01 1459110_at Myo9b BB344956 2.01 1416369_at Hiatl1 NM_133680 2.01 1456444_at Fbxo41 BB126849 2.01 1419975_at Scp2 C76618 2.01 1447889_x_at Gm50 BB172440 2.01 1431505_at Ppih AK008394 2.01 1418404_at Rad9 NM_011237 2.01 1458103_at Ncor1 AV325208 2.01 1428638_at Efhc2 BE956904 2.01 1423617_at Pdf AA790871 2.01 1419927_s_at Rabif AW549708 2.01 1455522_at Arhgef15 AW492648 2.01 1417930_at Nab2 NM_008668 2.01 1434207_at 2900057K09Rik BE570729 2.01 1424121_at Commd1 AB076722 2.00 1439874_at 9330102E08Rik BB075161 2.00 1434230_at Polb BG094331 2.00 1428213_at Nsmce4a AK010349 2.00 1450909_at Eif4e BB406487 2.00 1423789_at BC005624 BC005624 2.00 1428500_at 2210419D22Rik AK008985 2.00 1420106_at Siah1a AA982064 2.00 1434549_at Rab11a BI083615 2.00 1424685_at Exosc4 AK005101 2.00 1415864_at Bpgm NM_007563 2.00 1428901_at Dtwd2 AK004450 2.00 1436438_s_at Wdr22 BM234499 2.00 1424703_at Hemk1 BC011431 2.00 1419190_at Vti1a BC019386 2.00 1419460_at Rpp14 BI455861 2.00 1438289_a_at Sumo1 AV021595 2.00 1417033_at Ube2g2 AF296657 2.00 1457325_at Kcns2 BB442995 2.00 1452605_at Thnsl1 AV254642 2.00 1437132_x_at Nedd9 BB535494 2.00 1445631_at Tmem16c BE865226 2.00 1433774_x_at Cog1 AA238341 2.00 1428828_at Rpain AK010289 2.00 1419066_at Ier5l NM_030244 2.00 Fold change values (FCH) are given relative to MSN enrichment. Probe = Affymetrix probe identification; Symbol = official gene symbol.

5) Grouping Enriched Genes According to Biological Function

To group striatonigral- and striatopallidal-enriched genes according to biological function, statistically over-represented Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms associated with these genes were searched. GO terms delineate the known molecular function, biological process, as well as the cellular localization (component) for a particular gene (R. Bernardi, P. P. Pandolfi, Nat Rev Mol Cell Biol 8, 1006 (2007), while KEGG pathways summarize known molecular interaction and reaction networks. The most enriched striatonigral KEGG pathways included neuroactive ligand-receptor interaction, long-term depression, and MAPK signaling pathway. The most striatopallidal enriched KEGG pathways included pyrimidine metabolism, purine metabolism, and neuroactive ligand-receptor interaction. Comparison of striatonigral and striatopallidal cell data versus whole brain revealed many striatal-enriched KEGG pathways, including MAPK signaling pathway, long-term potentiation, and insulin signaling pathway. Striatonigral enriched GO molecular function terms included GTPase activity, calcium ion binding, and retinal binding; striatopallidal enriched GO molecular function terms included adrenoceptor activity, calcium channel activity, and rhodopsin-like receptor activity; striatal-enriched GO terms included zinc ion binding, protein serine/threonine kinase activity, and ubiquitin-protein ligase activity.

Example 11 Physiological Differences Between Striatonigral and Striatopallidal Cells

Differentially translated mRNAs discovered in previous examples immediately predict physiological differences between striatonigral and striatopallidal cells. For example, an mRNA selectively enriched in striatopallidal neurons is Gpr6 (S. Gong et al., Nature 425, 917-25 (Oct. 30, 2003).). Gpr6 encodes a G protein-coupled receptor for the lysophospholipid sphingosine 1-phosphate (S1P) (A. Ignatov, J. Lintzel, H. J. Kreienkamp, H. C. Schaller, Biochem Biophys Res Commun 311, 329-36 (Nov. 14, 2003)). In heterologous expression systems, S1P activation of Gpr6 receptors induces the release of Ca2+ from intracellular stores. As intracellular Ca2+ is a crucial regulator of neuronal physiology, whether selective enrichment of Gpr6 in striatopallidal neurons correlated with a differential response to S1P was investigated. To determine whether functional Gpr6 receptors were selectively expressed in striatopallidal neurons, BAC D2 striatopallidal or BAC D1 striatonigral medium spiny neurons (expressing soluble eGFP) were identified in brain slices and patch clamped (FIG. 30) (M. Day et al., Nat Neurosci 9, 251-9 (February, 2006)). Neurons were loaded with Alexa 594 (50 μM) to allow dendrites to be seen and with Fluo-4 (200 μM) to monitor intracellular Ca2+ concentration using 2-photon laser scanning microscopy (2PLSM). After allowing the dyes to equilibrate, neurons were imaged and a second pipette containing S1P (10 μM) was brought into close physical proximity to a dendrite, 60-80 microns from the soma (a and b). With the somatic membrane potential clamped at −70 mV, focal application of S1P consistently and reversibly increased dendritic Ca2+ levels in BAC D2 striatopallidal neurons (Kruskal-Wallis ANOVA, p<0.01, n=6) but not in BAC D1 striatonigral neurons (Kruskal-Wallis ANOVA, p>0.01, n=4). Depletion of intracellular Ca2+ stores with the Ca2+-ATPase inhibitor thapsigargin (10 μM) abolished the response to S1P in BAC D2 striatopallidal neurons (Kruskal-Wallis ANOVA, p>0.01, n=4; c and d), in agreement with previous work showing that Gpr6 receptors mobilize intracellular Ca2+ stores (D. S. Lim et al., Nature 404, 613 (2000). The Gpr6-dependent elevation of cytosolic Ca2+ seen in striatopallidal cells would be predicted to result in a decrease in threonine 34 phosphorylation of the centrally important regulatory protein DARPP-32, due to activation of the Ca2+ and calmodulin-dependent phosphatase calcineurin (A. Nishi, G. L. Snyder, A. C. Nairn, P. Greengard, J Neurochem 72, 2015-21 (May, 1999)) and/or inhibition of adenylyl cyclase type 5 (AC5; Adcy5) (C. E. Glatt, S. H. Snyder, Nature 361, 536-8 (Feb. 11, 1993); Y. Ishikawa et al., J Biol Chem 267, 13553-7 (Jul. 5, 1992)) This demonstrated the physiological consequence of the S1P-induced increase in cytosolic Ca2+ by measurement of the phosphorylation status of DARPP-32. A decrease in threonine 34 DARPP-32 phosphorylation was seen after 5 minutes of S1P treatment of striatal slices (1.04±0.17 normalized units at 0 minutes; 0.58 normalized units±0.24 at 5 minutes after S1P addition, one-tailed Mann-Whitney test, p=0.05, n=12), consistent with cytosolic Ca2+ elevation in D2 cells (which constitute approximately half of the medium-spiny neurons). These data confirm a prediction based on the BACarray translational profiling, demonstrate a strong and cell-type specific response of striatopallidal neurons to sphingosine 1-phosphate, and identify Gpr6 as a novel and important signaling component of striatopallidal, but not striatonigral, medium spiny neurons.

Example 12 Detection of Pharmacologically Induced Transcriptional Changes

1) Overview

In certain embodiments, responses to genetic, pharmacologic or environmental changes can be analyzed effectively in single cell types using translational profiling and molecular Phenotyping methods described herein. To define uncover novel physiological properties of striatal neurons, possible changes in mRNA expression of MSNs upon pharmacological perturbation of dopaminergic signaling was investigated.

Cocaine, a competitive inhibitor of the dopamine transporter, acts as a psychostimulant by elevating synaptic dopamine levels (M. C. Ritz, R. J. Lamb, S. R. Goldberg, M. J. Kuhar, Science 237, 1219-23 (Sep. 4, 1987); G. Di Chiara, A. Imperato, Proc Natl Acad Sci USA 85, 5274-8 (July, 1988)). Adult mice were treated acutely or chronically with cocaine or saline and used for BACarray profiling of striatonigral (D1) and striatopallidal (D2) MSNs.

2) Experimental Design

Quality control steps taken: three biological replicates were performed for each experiment. Arrays were only used if the Spearman correlation between the other arrays for that experiment was >75% but could be adjusted according to biological variation. Quantitative PCR reactions were performed to validate array results using an independent biological source and amplification methodology. The Experiment included 5 comparisons, with each D1 vs. D2 replicates performed on the same day, as described here in Table 14.

TABLE 14 Experimental Design for Effects of Cocaine Striatonigral Cells (D1) Striatopallidal Cells (D2) No treatment No treatment Acute Saline - Replicate 1 Acute Saline - Replicate 1 Acute Cocaine - Replicate 1 Acute Cocaine - Replicate 1 Chronic Saline - Replicate 1 Chronic Saline - Replicate 1 Chronic Cocaine - Replicate 1 Chronic Cocaine - Replicate 1

3) Animals and Sample Preparation:

Origin of biological sample: seven adult BACarray mice (four female, three male) were pooled for each replicate. Striatal tissue was taken between 7-9 weeks of age. Lines of mice used in each experiment: (Drd1a/eGFP-L10a) and (Drd2/eGFP-L10a).

Manipulation of biological sample: all mice were raised under 12 h dark/12 h light conditions, housed 5 mice per cage, with food and water ad libitum. All mice used in the study were heterozygous for the eGFP-L10a transgene; mice from each line had been crossed to wild-type Swiss-Webster (Taconic Farms) four times. Four heterozygous adult BACarray females and three heterozygous adult BACarray males were used for each study. Mice used for the baseline comparison of D1 versus D2 cells were used directly from the home cage. For all cocaine studies, BACarray mice 7-9 weeks of age were singly housed and intraperitoneally injected at the Rockefeller University Laboratory Animal Research Center (LARC) and were moved to the laboratory approximately 16 h before the final injection. For acute cocaine studies, all mice were injected with 100 μl saline (vehicle) once daily for eight days to habituate the mice to handling. On the ninth day mice were injected with a test dose of 20 mg/kg cocaine or 100 μl saline and the striata were harvested 4 h after this test injection. For chronic cocaine studies, mice were injected with 20 mg/kg cocaine or 100 μl saline once daily for fifteen days and the striata were harvested 4 h after the last injection.

Experimental factor values: D1; D2; Acute cocaine D1; Acute saline D1; Acute cocaine D2; Acute saline D2; Chronic cocaine D1; Chronic saline D1; Chronic cocaine D2; Chronic saline D2.

Results:

For the baseline comparison of striatonigral versus striatopallidal cells, a moderated two-tailed paired t-test was performed. The p-value of the moderated t-test was adjusted for multiple hypothesis testing, controlling the false discovery rate (FDR) using the Benjamini-Hochberg procedure. Genes that had an FDR less than 0.1 (10%) and fold change larger than 1.5 were then selected.

For the comparison of striatonigral and striatopallidal BACarray samples versus total brain minus striatum, GeneChip CEL files were imported into Gene spring GX 7.3.1, normalized with the GC-RMA algorithm (BACarray samples and whole brain minus striatum samples separately), and expression values on each chip were further normalized to the expression values of several positive control genes and to a constant value of 0.01. A moderated two-tailed paired t-test was then performed. The p-value of the moderated t-test was adjusted for multiple hypothesis testing, controlling the false discovery rate (FDR) using the Benjamini-Hochberg procedure. Genes that had an FDR less than 0.05 (5%) and fold change larger than 2 were selected

Upon clustering, all cocaine data exhibited a strong effect of the day the samples were prepared. A more complex model that could take into account and adjust for the day effect was utilized. A linear model was adjusted to each gene with factor Treatment (Saline vs Cocaine), Cell type (D1 vs D2), and Day (three different days for acute and chronic experiments). With this analysis, a large number of genes indeed displayed a Day factor. With this model fitted, all the hypotheses were tested (Cocaine vs Saline and its interaction with Cell type in both Acute and Chronic). For assessing differential expression in the comparisons of interest, the moderated t-statistic was used (G. K. Smyth, Stat Appl Genet Mol Biol 3, Article 3 (2004)). In this assessment, an empirical Bayes method is used to moderate the standard errors of the estimated log2-fold changes. This method was particularly useful for the analysis with only three replicates of each condition, as it delivered more stable inference and improved power. The p-value of the moderated t-test was adjusted for multiple hypothesis testing, controlling the false discovery rate (FDR) using the Benjamini-Hochberg procedure. Genes than have FDR less than 0.1 (10%) and fold change larger than 1.4 were selected.

To translate the gene expression data into functional profiles, Gene Ontology (GO) analysis and Pathway analysis was utilized to look for enriched GO terms and Pathways in the set of differentially expressed genes. Analysis of GO annotations was performed using the R packages GOstats and GOtools. To find those GO terms that were over represented in the list of genes in question, for each specific term in a given ontology (Biological Process, Molecular Function, Cellular Component), the proportion of genes in the list that falls into the GO term is compared with the proportion in the whole set of genes in the Mouse Genome 430 2.0 array using a conditional hypergeometric test. A conditional instead of the classical hypergeometric test was utilized to address concerns about the hierarchical structure of GO terms (S. Falcon, R. Gentleman, Bioinformatics 23, 257-8 (2007); A. Alexa, J. Rahnenfuhrer, T. Lengauer, Bioinformatics 22, 1600-7 (2006)). The conditional test uses the relationship among GO terms to decorrelate results. GO with p values <0.1 and with more than one gene were considered. To find overrepresented Pathways, a classical hypergeometric test was utilized to compare the proportion of genes among those that are differentially expressed (Up and Down together) that belong to a certain Pathway, using the whole Mouse Genome 430 2.0 array gene set as the universe for the comparison. A Pathways annotation package available at Bioconductor.org (version 1.16.0) was used. The KEGG Pathways terms in that package were obtained from KEGG: file transfer protocol address: genome.ad.jp/pub/kegg/tarfiles/pathway.tar.gz, build release 41.1, Feb. 1, 2007.

From this analysis hundreds of genes were identified whose expression was increased or decreased in each cell type in response to cocaine (

Table 15 and Table 16). Various genes whose expression has been reported to be affected by cocaine administration were identified, including: Cartpt (J. Douglass, A. A. McKinzie, P. Couceyro, J Neurosci 15, 2471-81 (March, 1995) (up in acute D1; Table S12); Fosb (B. Hope, B. Kosofsky, S. E. Hyman, E. J. Nestler, Proc Natl Acad Sci USA 89, 5764-8 (Jul. 1, 1992)) (up in acute D1, acute D2, and chronic D1;

Table 15 and Table 16); Homerl (P. R. Brakeman et al., Nature 386, 284-8 (Mar. 20, 1997)) (up in acute D1, acute D2, chronic D1, and chronic D2;

Table 15 and Table 16); Per2 (V. Yuferov et al., Synapse 48, 157-69 (Jun. 15, 2003)) (up in acute D2, chronic D1, chronic D2;

Table 15 and Table 16); Vamp2 (C. A. McClung, E. J. Nestler, Nat Neurosci 6, 1208-15 (November, 2003)) (up in chronic D1; Table S13); Kcnd2 (C. A. McClung, E. J. Nestler, Nat Neurosci 6, 1208-15 (November, 2003)) (up in chronic D1; Table 16); and Zfp64 (C. A. McClung, E. J. Nestler, Nat Neurosci 6, 1208-15 (November, 2003)) (up in acute D2, down in chronic D2;

Table 15 and Table 16).

TABLE 15 Gene translation changes upon acute cocaine treatment FCH FCH Probe Symbol GenBank D1 D2 1459589_at Cryl1 C85932 6.86 0.37 1459589_at Vcan BM251152 3.06 0.97 1423287_at Cbln1 AA016422 2.88 1.36 1434950_a_at Armc8 BE995635 2.87 0.78 1422533_at Cyp51 NM_020010 2.84 0.92 1438752_at A230058F20Rik AV327739 2.80 1.67 1428601_at 1700003E16Rik AK005628 2.69 0.76 1420901_a_at Hk1 NM_010438 2.38 1.03 1460330_at Anxa3 AW702161 2.36 1.03 1444137_at A430108G06Rik AW120610 2.36 1.03 1443699_at Ptprk BE957383 2.31 0.63 1443998_at BE980823 2.28 1.02 1422904_at Fmo2 NM_018881 2.26 0.85 1456133_x_at Itgb5 BB543646 2.15 1.31 1452226_at Rcc2 AV122997 2.11 1.28 1433836_a_at 8430408G22Rik AV365503 2.11 0.72 1435872_at Pim1 BE631223 2.11 2.83 1436387_at Homer1 BB398124 2.10 2.41 1422134_at Fosb NM_008036 2.09 2.49 1451554_a_at Aph1a BC012406 2.07 0.89 1431715_a_at Lig3 AK010719 2.06 0.59 1424393_s_at Adhfe1 BC026584 1.97 1.04 1437284_at Fzd1 BB259670 1.97 1.19 1430133_at Tbc1d8b AK014817 1.96 1.31 1435458_at Pim1 AI323550 1.96 2.90 1430600_at Cmtm5 AK013666 1.96 1.71 1440116_at D630045J12Rik AW558117 1.94 1.17 1458284_at Ptbp1 BM195499 1.93 0.87 1425585_at Med12 AF071310 1.92 0.99 1418322_at Crem AI467599 1.89 1.85 1451411_at Gprc5b BC020004 1.88 1.12 1430510_at B130050I23Rik AK009699 1.87 1.09 1436682_at Tmsb10 AW259435 1.87 0.88 1451440_at Chodl AF311699 1.87 0.91 1452003_at Rbm14 BC010294 1.86 0.81 1453787_at Txndc13 AK015667 1.83 0.91 1432466_a_at Apoe AK019319 1.82 1.58 1415851_a_at Impdh2 M33934 1.81 0.87 1460061_at Adra1a AV342748 1.81 1.42 1427465_at Atp1a2 BC025807 1.81 1.08 1426225_at Rbp4 U63146 1.80 1.48 1418520_at Tgoln1 NM_009443 1.80 1.11 1417782_at Lass4 BB006809 1.79 1.13 1441334_at BB528600 1.78 0.94 1423278_at Ptprk AI893646 1.77 1.21 1421571_a_at Ly6c NM_010741 1.77 1.19 1428298_at 1700029G01Rik AK006490 1.77 0.60 1446147_at BB436856 1.77 0.80 1437264_at BC051142 AV278321 1.77 1.22 1455925_at Prdm8 AV349236 1.76 1.11 1434110_x_at Mup1 BF322785 1.76 0.72 1422825_at Cartpt NM_013732 1.76 1.29 1428054_at Slc8a2 AF503502 1.76 0.92 1455277_at Hhip BB088162 1.75 0.92 1457567_at Nagk BM938681 1.75 1.07 1421276_a_at Dst NM_134448 1.74 1.42 1434606_at Erbb3 BF140685 1.74 1.60 1442554_s_at Kalrn BG066934 1.74 0.65 1435559_at Myo6 BB200233 1.73 1.48 1425546_a_at Trf AF440692 1.73 1.50 1422053_at Inhba NM_008380 1.72 2.10 1420158_s_at Abcf1 AA408356 1.72 0.83 1427692_a_at Cask Y17137 1.72 1.08 1423700_at Rfc3 BC026795 1.72 1.12 1424805_a_at 1110039B18Rik BC027046 1.72 1.02 1429628_at 6330407J23Rik AK012378 1.71 0.93 1437920_at Epha5 AI854630 1.70 1.29 1417509_at Rnf19 AF120206 1.70 1.26 1456384_at Nlgn3 BB308872 1.70 1.03 1440862_at Tmem181 BB629079 1.70 1.24 1455492_at B330016D10Rik BB313577 1.69 1.40 1448502_at Slc16a7 NM_011391 1.68 1.10 1453583_at Zzef1 AI466222 1.68 0.80 1420652_at Ate1 BE309332 1.68 0.71 1420720_at Nptx2 NM_016789 1.68 1.43 1437147_at Gabrg2 AV348821 1.67 1.23 1435749_at Gda AW911807 1.67 1.11 1437308_s_at F2r AV024285 1.67 0.90 1416589_at Sparc NM_009242 1.66 1.58 1450708_at Scg2 NM_009129 1.66 1.59 1425227_a_at Atp6v0a1 BC001995 1.66 1.19 1433607_at Cbln4 AV343573 1.66 1.56 1450976_at Ndrg1 AI987929 1.66 1.25 1429475_at 2810457I06Rik AK013361 1.66 1.83 1447005_at BE991564 1.65 0.83 1452754_at Creld2 AK017880 1.65 1.42 1449411_at Dscam NM_031174 1.65 0.99 1428922_at 1200009O22Rik AK004681 1.65 1.56 1421959_s_at Adcy3 AF458089 1.64 1.13 1453056_at Slc16a13 AK005699 1.64 1.08 1455061_a_at Acaa2 BB718075 1.64 1.66 1439764_s_at Igf2bp2 BB098431 1.64 1.10 1451699_at EG668468 BC024416 1.64 0.78 1456825_at Wdr33 BB482118 1.63 0.98 1438725_at Thrap1 BB212816 1.63 1.17 1448384_at Pofut2 BC018194 1.63 1.59 1444690_at Epha5 BE951744 1.63 1.29 1415885_at Chgb NM_007694 1.63 1.54 1442788_at 2600003E23Rik BB698513 1.63 0.98 1449289_a_at B2m BF715219 1.63 1.17 1418132_a_at D7Wsu128e BC011313 1.62 0.79 1427539_a_at Zwint BC013559 1.62 1.42 1450193_at Hcn1 NM_010408 1.62 1.45 1457536_at Gpc5 BB171986 1.62 1.11 1420564_at Insrr NM_011832 1.62 0.81 1460583_at Golt1b AV349681 1.62 1.08 1425110_at Sorcs3 AK018111 1.62 1.19 1421535_a_at Pde4a NM_019798 1.62 0.75 1452768_at Tex261 AK013971 1.62 0.97 1438750_at Atrx BB425841 1.61 1.05 1443898_at Bcl7c BB826733 1.61 1.08 1453531_at Zfp248 AK012874 1.61 1.01 1418260_at Hunk NM_015755 1.61 0.72 1438151_x_at Zdhhc14 BB544336 1.60 1.26 1425899_a_at Itsn1 AF132478 1.60 0.89 1418253_a_at Hspa41 NM_011020 1.60 1.12 1428136_at Sfrp1 AK008943 1.60 1.08 1429256_at Gtl2 AU067739 1.60 0.94 1418350_at Hbegf L07264 1.60 1.48 1436785_a_at Sec14l3 AV024133 1.60 0.69 1451289_at Dcamkl1 AW105916 1.59 1.15 1425725_s_at Ppp2r5c BF136532 1.59 1.30 1435568_at AK129128 AW545855 1.59 1.12 1449151_at Pctk3 NM_008795 1.59 1.19 1436129_at 4930523C11Rik AV255799 1.58 0.84 1460293_at Freq AU079041 1.58 0.78 1428488_at Pigk BB646655 1.58 1.24 1422683_at Irak1bp1 NM_022986 1.58 0.85 1434856_at Ankrd44 AV256780 1.58 1.17 1439015_at Gfra1 AV221299 1.58 1.15 1434987_at Aldh2 AI462635 1.58 0.79 1436454_x_at Fen1 BB393998 1.57 0.91 1449799_s_at Pkp2 AA516617 1.57 2.42 1419557_a_at Tmem9 NM_025439 1.57 1.28 1423668_at Zdhhc14 BC021423 1.57 1.75 1427284_a_at Ttpa AU019171 1.57 0.95 1434270_at Nptxr AV221896 1.57 0.96 1418318_at Rnfl28 AK004847 1.57 1.16 1434233_at 2610030H06Rik BB429625 1.57 0.72 1421111_at Rybp NM_019743 1.57 1.00 1435109_at Tmem175 AW123256 1.57 1.12 1457956_at Camta1 BB777776 1.57 2.10 1418149_at Chga NM_007693 1.57 1.66 1428336_at Agpat4 AK005139 1.56 0.93 1449972_s_at Zfp97 NM_011765 1.56 0.91 1451611_at Hrasls3 BC024581 1.56 1.31 1451961_a_at Mbp L07509 1.56 1.87 1436094_at Vgf BF458396 1.56 1.41 1435904_at Eif2c3 BB355034 1.55 1.44 1450117_at Tcf3 NM_009332 1.55 1.36 1441127_at BB460036 1.54 1.23 1442905_at Arf4 BB066458 1.54 1.14 1448601_s_at Msx1 BC016426 1.54 1.08 1436898_at Sfpq BI738328 1.54 1.00 1424581_at Stac2 BC024864 1.54 1.28 1451406_a_at Pcsk5 BC013068 1.54 1.16 1425415_a_at Slc1a1 U75214 1.54 1.26 1441165_s_at Clstn2 AI448973 1.54 1.04 1416371_at Apod NM_007470 1.54 1.52 1429463_at Prkaa2 BB612385 1.54 1.01 1438118_x_at Vim AV147875 1.54 0.84 1417502_at Tspan7 AF052492 1.53 1.09 1435145_at Cadm2 BB333386 1.53 1.30 1417764_at Ssr1 BG077348 1.53 0.97 1436805_at 2810457I06Rik BG076317 1.53 1.51 1452429_s_at Abcf1 BF236176 1.53 1.34 1423643_at Ddx39 BC020134 1.53 1.44 1425536_at Stx3 D29797 1.53 0.73 1451386_at Blvrb BC027279 1.53 1.19 1456271_at Gm944 BB039066 1.53 0.90 1434199_at Gm323 BM898903 1.53 1.21 1450983_at Akap8 BG069776 1.52 1.11 1452905_at Gtl2 AV015833 1.52 0.67 1449381_a_at Pacsin1 BI731319 1.52 1.08 1445268_at AW550459 1.52 1.09 1425568_a_at Tmem33 BC005562 1.51 1.05 1450699_at Selenbp1 NM_009150 1.51 1.35 1418104_at Nrip3 NM_020610 1.51 1.90 1448050_s_at Map4k4 BF450398 1.51 2.05 1452835_a_at Polrmt AK003792 1.51 1.44 1423070_at Rpl21 BG922742 1.51 1.13 1430798_x_at Mrpl15 AV306676 1.51 1.76 1439380_x_at Gtl2 BB093563 1.51 0.91 1460170_at Ext2 NM_010163 1.51 1.17 1450965_at Tex261 BF181445 1.51 1.24 1456389_at Zeb2 BB244754 1.51 0.90 1421374_a_at Fxyd1 NM_019503 1.51 0.92 1450351_a_at Clip1 NM_019765 1.51 1.10 1424133_at Tmem98 BC011208 1.50 1.24 1434585_at Tulp4 BB667130 1.50 1.45 1429591_at Tacc1 BE862546 1.50 1.45 1418816_at Chmp1b BG073376 1.50 1.42 1424950_at Sox9 BC024958 1.50 0.97 1460743_at Tigd5 BB553398 1.49 1.22 1439559_at 1700040D17Rik AV260432 1.49 1.08 1460331_at Tm9sf2 BB747462 1.49 1.40 1435703_at AW045947 1.49 1.64 1436325_at Rora BB306272 1.49 0.76 1453334_at B230216N24Rik AK021003 1.49 1.60 1423859_a_at Ptgds AB006361 1.49 1.06 1438706_at 6430517E21Rik BB309608 1.49 1.27 1415806_at Plat NM_008872 1.49 1.56 1429055_at 4930506M07Rik BB559293 1.48 1.10 1431755_a_at Ccdc49 BC021478 1.48 1.05 1437495_at Mbtps2 AV272026 1.48 1.16 1421487_a_at Nck1 NM_010878 1.48 1.40 1426781_at Tyw1 BF021045 1.48 1.05 1426852_x_at Nov X96585 1.48 1.53 1436137_at Slc6a17 AV340147 1.48 1.17 1447759_x_at Ccdc22 BB345575 1.48 0.73 1418045_at Inpp1 NM_008384 1.48 1.52 1448242_at Sec61a1 BC003707 1.48 1.20 1424861_at Zbtb24 BM200153 1.48 0.86 1437142_a_at Pigo BB546713 1.48 1.14 1423796_at Sfpq AY034062 1.47 1.40 1458622_at Ntrk2 AW121243 1.47 1.29 1416032_at Tmem109 AW494906 1.47 0.95 1455476_a_at Gse1 BE686504 1.47 1.55 1426311_s_at Zdhhc5 AV241525 1.47 1.51 1418456_a_at Cxcl14 AF252873 1.47 0.92 1455965_at Adamts4 BG064671 1.47 1.26 1455993_at Odz4 BG071076 1.47 1.25 1431385_a_at Mbtps1 AK002809 1.47 1.09 1450172_at Pknox1 AA409923 1.47 1.77 1415901_at Plod3 NM_011962 1.47 1.16 1420838_at Ntrk2 AK018789 1.47 1.61 1455908_a_at Scpep1 AV102733 1.47 0.82 1418402_at Adam19 NM_009616 1.46 1.03 1434505_a_at 6430548M08Rik BF465109 1.46 0.87 1438059_at 1700011I03Rik BM116248 1.46 0.80 1434235_at Slc20a2 BB765719 1.46 1.37 1420845_at Mrps2 AV031454 1.46 1.28 1423566_a_at Hsp110 BI499717 1.46 1.18 1436919_at Trp53i11 BQ175031 1.46 0.88 1428403_at 2410025L10Rik AV330483 1.46 1.43 1430568_at Zc3h13 AW539538 1.46 1.87 1456620_at Gnptab BE944732 1.46 1.18 1458384_at AW491514 1.46 0.85 1437879_at Alg10b BG071776 1.45 0.97 1416702_at Serpini1 NM_009250 1.45 1.63 1451352_s_at Mta3 BC022124 1.45 1.22 1451369_at Commd5 BC025891 1.45 0.67 1427994_at Cd300lf BM230330 1.45 0.99 1460098_at LOC629517 BB363968 1.45 1.46 1439408_a_at Pparbp AV100992 1.45 1.17 1449286_at Ntng1 NM_030699 1.45 1.32 1452624_at Ctnna2 BM900077 1.45 1.78 1437092_at Clip4 AV042829 1.45 1.03 1437966_at Prrt3 AV329802 1.45 1.41 1449164_at Cd68 BC021637 1.45 1.23 1456727_a_at Csnk1d AW413676 1.44 0.88 1435993_at 4930470G03Rik BB027219 1.44 1.75 1416588_at Ptprn NM_008985 1.44 1.27 1419646_a_at Mbp NM_010777 1.44 1.52 1449730_s_at Fzd3 AU020229 1.44 1.08 1452203_at Obfc2a AV313559 1.44 1.42 1426620_at Chst10 BB549997 1.44 1.34 1427021_s_at Eif3s5 AK002778 1.44 0.98 1423423_at Pdia3 BF319868 1.43 1.11 1455009_at Cpd AW550842 1.43 1.35 1451071_a_at Atp1a1 BC025618 1.43 0.94 1418331_at 1110031I02Rik NM_025402 1.43 1.05 1448603_at Srpk2 NM_009274 1.43 1.37 1421816_at Gsr AK019177 1.43 1.23 1434102_at Nfib BI687652 1.43 0.86 1419938_s_at Arhgef17 AW558066 1.43 1.21 1455917_at Ntrk3 BB456963 1.43 0.93 1441880_x_at U2afl14 BB009770 1.43 1.19 1448397_at Gjb6 BC016507 1.43 1.62 1457467_at Pknox1 BB362579 1.43 1.03 1436097_x_at Arhgap9 BB327418 1.43 0.44 1420419_a_at Otof NM_031875 1.43 0.97 1438458_a_at Sfpq BF224766 1.42 1.49 1424781_at Reep3 AK005026 1.42 0.97 1460205_at Dcakd NM_026551 1.42 1.05 1421491_a_at Tmem49 NM_029478 1.42 1.60 1449405_at Tns1 NM_027884 1.42 1.31 1455122_at Ptchd2 AI666776 1.42 1.01 1456379_x_at Dner BB038556 1.42 1.27 1421360_at Inpp4a NM_030266 1.42 1.27 1450010_at Hsd17b12 AK012103 1.42 1.92 1436057_at Gtl2 BM117428 1.42 1.23 1449885_at Tmem47 NM_138751 1.42 2.12 1426280_at AV249152 AF424701 1.42 1.13 1438165_x_at Vat1 BB559097 1.42 0.88 1440258_at Kcnq2 BE951512 1.41 1.35 1448113_at Stmn1 BC010581 1.41 0.93 1428793_at Slc36a1 AK017918 1.41 1.43 1438957_x_at Cds2 BB027654 1.41 1.19 1438975_x_at Zdhhc14 AV361868 1.41 1.49 1448957_at Rbpsuh NM_009035 1.41 1.14 1429257_at Gtl2 AU067739 1.41 0.81 1447774_x_at 5730469M10Rik AV332575 1.41 1.14 1426767_at 3230401M21Rik AK014328 1.41 1.87 1428586_at Tmem41b BB823331 1.41 1.00 1441737_s_at Rassf1 BB757103 1.41 0.90 1450872_s_at Lipa AI596237 1.41 1.18 1417946_at Abhd3 NM_134130 1.41 1.26 1439776_at Ogfod1 BE956398 1.41 1.31 1453581_at Cep170 BB361436 1.41 1.30 1452232_at Galnt7 AV302406 1.41 1.55 1440143_at Pigz AI413722 1.41 0.94 1460327_at Gpr88 BE947345 1.40 1.40 1423294_at Mest AW555393 1.40 1.79 1445605_s_at 4921533L14Rik AI835491 1.40 1.06 1450268_at Fign NM_021716 1.40 1.68 1417605_s_at Camk1 NM_133926 1.40 0.94 1423950_at Kcnab3 BC017518 1.40 1.38 1425301_at Ncam2 AF001287 1.40 0.87 1417149_at P4ha2 NM_011031 1.40 1.53 1420969_at Btbd14b NM_025788 1.39 0.69 1448673_at Pvrl3 NM_021495 1.39 0.67 1451718_at Plp1 BB768495 1.38 1.44 1423826_at Noc41 BC024616 1.38 1.45 1437614_x_at Zdhhc14 AV223474 1.38 1.46 1425474_a_at Vps39 BC007479 1.38 1.61 1430294_at Ssbp1 AK013425 1.38 1.43 1448216_at Syngr3 NM_011522 1.38 1.45 1423192_at Pspc1 BB590675 1.37 1.84 1456783_at 9330107J05Rik BB075402 1.37 1.44 1426449_a_at Pja1 BM199789 1.37 1.45 1437857_at Dpy19l3 AV367203 1.37 1.45 1418349_at Hbegf L07264 1.37 1.62 1444327_at Edd1 BF224640 1.37 1.63 1431802_a_at D5Wsu178e AK014530 1.36 1.47 1435553_at Pdzd2 AV376136 1.36 1.83 1436201_x_at Mbp BB761376 1.36 1.51 1426837_at Metap1 BG064851 1.36 1.77 1417110_at Man1a BB070019 1.35 1.45 1444661_at Gpr26 BB247791 1.35 1.96 1447672_x_at Pskh1 BB102342 1.35 0.64 1417954_at Sst NM_009215 1.35 1.41 1444614_x_at 6430537H07Rik AV258949 1.35 0.33 1435456_at Ttc28 AW552254 1.35 1.44 1451726_at Mtmr6 BC020019 1.34 1.66 1421201_a_at Tro AF241244 1.34 1.50 1442565_at Pcdh10 BB174272 1.34 0.70 1416271_at Perp NM_022032 1.34 0.70 1425656_a_at Baiap2 AF390178 1.34 1.41 1422629_s_at Shroom3 NM_015756 1.34 1.50 1435158_at Rbm12b BB796313 1.34 1.43 1434192_at Zzef1 BQ175699 1.34 1.46 1454608_x_at Ttr BG141874 1.33 0.16 1425329_a_at Cyb5r3 AF332060 1.33 1.76 1439406_x_at Fars2 BB530332 1.33 0.71 1419873_s_at Csflr AI323359 1.33 1.69 1454768_at Kcnfl AV337635 1.33 1.40 1432000_a_at Dedd AK006814 1.32 1.51 1436444_at 6030405A18Rik AV319257 1.32 1.53 1433748_at Zdhhc18 BG073303 1.32 1.45 1418941_at Pcdhb22 NM_053147 1.32 1.54 1437472_at Unc13a BB429098 1.31 1.43 1430193_at Casc5 BF021255 1.31 0.55 1438619_x_at Zdhhc14 BB318221 1.31 1.44 1456070_at 5430405N12Rik AI507538 1.31 1.46 1450905_at Plxnc1 BB476707 1.31 1.54 1451680_at Srxn1 BC011325 1.31 1.58 1425517_s_at Ogt AF363030 1.31 1.44 1427893_a_at Pmvk BI713896 1.30 1.41 1417279_at Itpr1 NM_010585 1.30 1.49 1422621_at Ranbp2 BM507707 1.30 1.54 1436231_at AU067665 AU067665 1.30 1.47 1448883_at Lgmn NM_011175 1.30 1.46 1456833_at Gpr17 BB353220 1.29 1.56 1436611_at Slc39a12 AW046938 1.29 2.04 1422018_at Hivep2 NM_010437 1.29 1.56 1437067_at Phtf2 BM228625 1.29 1.43 1439289_s_at 0710005I19Rik BB197516 1.29 0.62 1434891_at Ptgfrn AV253087 1.28 1.43 1416445_at 2810405J04Rik NM_133747 1.28 1.68 1438078_at BF730550 1.28 1.48 1448139_at Mlc1 NM_133241 1.28 2.12 1416262_at Tmem19 AK018383 1.28 1.53 1418540_a_at Ptpre U35368 1.28 1.43 1416178_a_at Plekhb1 NM_013746 1.28 1.47 1451163_at Tinf2 AF214013 1.28 1.57 1451499_at Cadps2 AF000969 1.27 0.60 1416933_at Por NM_008898 1.27 1.48 1424112_at Igf2r BG092290 1.27 1.59 1451133_s_at Tmem168 BC007160 1.27 1.45 1449209_a_at Rdh11 AB030503 1.27 1.54 1416749_at Htra1 NM_019564 1.27 1.85 1431711_a_at 9030409G11Rik AK018497 1.27 1.67 1440779_s_at Slc5a9 BB502156 1.27 0.63 1418007_at 1810007M14Rik BM932567 1.27 1.42 1428834_at Dusp4 AK012530 1.27 1.55 1452757_s_at Hba-a1 AK011116 1.27 1.53 1419063_at Ugt8a NM_011674 1.27 1.47 1456110_at Ankrd11 BB320513 1.27 1.46 1418626_a_at Clu NM_013492 1.27 1.70 1428031_at Mchr1 BE647763 1.26 1.49 1454654_at Dirc2 BG069395 1.26 1.47 1423574_s_at Srd5a2l BB206480 1.26 1.49 1450971_at Gadd45b AK010420 1.26 1.98 1452377_at Mll1 AK017541 1.26 1.57 1458979_at BB332816 1.26 1.54 1444307_at AW491448 1.26 2.53 1420502_at Sat1 NM_009121 1.26 1.47 1439041_at Slc39a10 BM239325 1.26 1.56 1428387_at Acsl3 AK012088 1.25 1.55 1458842_at AV340888 1.25 1.40 1424482_at Arhgef7 AF343877 1.25 1.46 1418169_at Zcchc14 BB223737 1.25 1.45 1427931_s_at Pdxk BG063905 1.25 1.60 1429183_at Pkp2 AK005020 1.25 1.57 1434510_at Papss2 BF780807 1.25 1.68 1424098_at Elovl7 BC005602 1.25 1.41 1415857_at Emb BG064842 1.25 1.61 1418539_a_at Ptpre U35368 1.24 1.45 1417988_at Resp18 NM_009049 1.24 1.60 1444226_at Foxo3a W07885 1.24 1.81 1450326_at Shc3 NM_009167 1.24 1.44 1438402_at 9630050M13Rik BB131315 1.24 1.51 1439279_at 3110007F17Rik AV339304 1.24 1.45 1415743_at Hdac5 NM_010412 1.24 1.42 1417947_at Pcna BC010343 1.24 1.53 1423795_at Sfpq AY034062 1.24 1.53 1428112_at Armet AK014338 1.24 1.46 1437159_at Eif2c5 AW553762 1.24 1.55 1429517_at Zfyve20 BC017622 1.23 2.63 1448460_at Acvr1 NM_007394 1.23 1.44 1453109_at Arsk AK013194 1.23 0.67 1421010_at Mobp NM_008614 1.23 1.78 1434761_at Lrrtm3 BM224801 1.23 1.85 1449773_s_at Gadd45b AI323528 1.23 1.55 1437633_at Ankrd11 BB320513 1.23 1.46 1421190_at Gabrb3 BB367779 1.23 1.55 1434595_at Trim9 BQ174474 1.23 1.69 1459737_s_at Ttr AA408768 1.23 0.18 1428350_at 2310061F22Rik AV091287 1.23 1.70 1429683_at 5830472M02Rik BG094398 1.22 1.51 1457125_at AV266261 1.22 1.86 1425351_at Srxn1 BC011325 1.22 1.46 1435245_at Gls2 AI195532 1.22 1.43 1431645_a_at Gdi2 AK013104 1.22 1.52 1434759_at Lrrtm3 BM224801 1.22 1.52 1460206_at Grasp NM_019518 1.22 1.67 1437880_at Lbxcor1 AV273001 1.22 0.67 1428765_at Gtl2 AV015833 1.21 0.66 1451895_a_at Dhcr24 BC004738 1.21 1.44 1428781_at Dmkn BI452905 1.21 1.80 1417238_at Ewsr1 NM_007968 1.21 1.85 1425396_a_at Lck BC011474 1.21 0.62 1438116_x_at Slc9a3r1 BB805362 1.21 1.70 1421948_a_at Ccdc123 BB042564 1.20 1.50 1438194_at 2900019G14Rik AW488243 1.20 1.42 1439333_at Kcnv1 BB078927 1.20 1.60 1437573_at BF018351 1.20 0.61 1455291_s_at Znrf2 BG071922 1.20 1.48 1418195_at Galnt10 BG965198 1.20 1.61 1425977_a_at Slk AF112855 1.20 1.85 1427930_at Pdxk BG063905 1.20 1.82 1421199_at Dlg2 BB622767 1.20 1.47 1442400_at Prickle1 BF472052 1.19 0.61 1427077_a_at Ap2b1 AV271093 1.19 1.52 1436501_at Mtus1 BB747681 1.19 1.45 1456388_at Atp11a AV378604 1.19 1.55 1427304_at Vps18 AW541109 1.19 1.52 1427889_at Spna2 AK011566 1.19 1.73 1448353_x_at Rpn1 NM_133933 1.19 1.42 1450538_s_at Mcpt9 AY007568 1.19 0.56 1451078_at 2510039O18Rik BC012878 1.19 1.54 1435947_at 2810455D13Rik BQ175669 1.18 1.44 1428908_at 2600011C06Rik BG228787 1.18 1.69 1423553_at Dnajb3 AK005688 1.18 0.71 1434763_at Tmem142b BF457736 1.18 1.45 1444509_at AI847347 1.18 1.49 1452094_at P4ha1 AI314028 1.18 1.68 1427308_at Dab1 BB644109 1.18 1.57 1430700_a_at Pla2g7 AK005158 1.18 2.25 1436329_at Egr3 AV346607 1.18 1.43 1438565_at A830010M20Rik BB464727 1.18 1.88 1430834_at BB359379 1.18 0.66 1436811_at Kctd3 AV232970 1.17 1.40 1426278_at Ifi27 AY090098 1.17 1.52 1420491_at Eif2s1 BC016497 1.17 1.50 1426910_at Pawr BB398886 1.17 0.68 1455110_at Gabpb2 AW536205 1.17 1.61 1460043_at BB357628 1.17 2.10 1424734_at Rasgrf1 AF169826 1.17 1.45 1453796_a_at Ergic2 AK016275 1.17 1.51 1434893_at Atp1a2 AI845177 1.17 1.59 1420402_at Atp2b2 NM_009723 1.16 1.56 1448154_at Ndrg2 NM_013864 1.16 1.92 1420022_s_at Suz12 AU022339 1.16 1.48 1424077_at Gdpd1 AK016023 1.16 1.95 1424869_at Dhrs7b BC003479 1.16 1.43 1457456_at Ttc9b AI481735 1.16 0.68 1460716_a_at Cbfb NM_022309 1.16 0.71 1427593_at Trim8 BB620112 1.16 1.52 1426959_at Bdh1 BF322712 1.16 1.88 1452034_at Prepl BB005298 1.16 1.45 1426381_at Pprc1 BM199989 1.15 1.54 1421884_at Sos1 BB471450 1.15 1.43 1448908_at Ppap2b NM_080555 1.15 1.65 1455698_at Tloc1 BQ175470 1.15 1.42 1428168_at Mpzl1 AK003513 1.15 1.51 1426770_at Pex5 AJ416473 1.15 1.69 1428540_at 3321401G04Rik BE944524 1.15 1.41 1455632_at Gnb5 BE446953 1.15 2.01 1424684_at Rab5c BC023027 1.15 0.69 1452829_at Cad AK010453 1.15 1.41 1455628_at Epb4.1l4b BB317849 1.14 1.46 1430990_s_at Mrpl44 AK019986 1.14 1.50 1430066_at 4930589M24Rik AK014044 1.14 1.49 1434360_s_at Ptprg AI596632 1.14 1.58 1415964_at Scd1 NM_009127 1.14 1.55 1439254_at Akap13 BE981392 1.14 0.64 1425617_at Dhx9 U91922 1.14 1.67 1450777_at Xrn2 NM_011917 1.14 1.46 1417170_at Lztfl1 NM_033322 1.14 1.52 1439324_at D230004J03Rik BB282778 1.14 0.66 1454915_at Rab3gap2 BB032142 1.14 1.71 1424146_at Gpr37l1 AB016602 1.14 1.58 1416562_at Gad1 AF326547 1.14 1.56 1422779_at Smpd3 NM_021491 1.14 1.63 1455468_at BB768758 1.14 1.43 1427016_at 4932438A13Rik BC027125 1.14 1.41 1427138_at 0610010D24Rik AW556861 1.14 1.41 1423475_at Cnnm2 BB278418 1.13 1.57 1423479_at Nol11 AK005123 1.13 1.53 1451285_at Fus AF224264 1.13 2.12 1456312_x_at Gsn AV224521 1.13 2.63 1436515_at E030004N02Rik BB529913 1.13 1.44 1442714_at D130037M23Rik BB322545 1.13 1.93 1454811_a_at Serinc1 AV026664 1.13 1.55 1420262_at AV291437 1.13 0.67 1454890_at Amot BG067039 1.13 1.40 1454784_at Hs3st2 AV340742 1.13 1.44 1426809_at C430004E15Rik BF582414 1.12 1.83 1453122_at 4921533L14Rik AK019549 1.12 1.44 1416794_at Arl6ip2 NM_019717 1.12 1.61 1458417_at Jmjd1b BB712040 1.12 1.56 1437295_at Pkn2 AI507382 1.12 1.57 1417475_at Atp13a1 NM_133224 1.12 1.48 1455459_at Prdm15 BB213846 1.12 1.44 1417602_at Per2 AF035830 1.12 1.88 1426991_at 1810048J11Rik AV273819 1.12 1.40 1441921_x_at Esrrb BB386239 1.12 0.62 1450480_a_at Gprk6 AF040749 1.12 1.42 1457791_at B830008H07Rik BB333194 1.12 1.62 1426897_at Rcc2 AV122997 1.11 1.42 1424161_at Ddx27 BC011321 1.11 1.54 1429514_at Ppap2b AW111876 1.11 1.60 1453851_a_at Gadd45g AK007410 1.11 1.41 1424373_at Armcx3 AK004598 1.11 1.52 1435890_at AW228836 BB795103 1.11 1.54 1457664_x_at C2 AV227574 1.11 1.45 1427888_a_at Spna2 AK011566 1.11 1.45 1425713_a_at Rnf146 BC019182 1.11 1.45 1423269_a_at Nedd41 BB663717 1.10 1.66 1451308_at Elovl4 BB829575 1.10 1.44 1426600_at Slc2a1 BM209618 1.10 2.23 1421845_at Golph3 AV174110 1.10 1.49 1430034_at Cct4 BE648588 1.10 1.66 1417775_at Rpo1-4 BB729239 1.10 1.43 1453195_at Sdccag3 BF319466 1.10 1.45 1455395_at Oxsm BB446073 1.10 0.70 1442764_at Suv420h1 BE456272 1.09 1.99 1426542_at Endod1 BF168366 1.09 1.41 1455023_at N28178 BB171181 1.09 1.43 1451351_at Ttc13 BC017545 1.09 1.43 1421100_a_at Dab1 NM_010014 1.09 1.46 1424036_at Prpf6 BC014869 1.09 1.43 1440397_at BB464523 1.09 1.48 1435717_at 4833428C12Rik AV250971 1.09 1.48 1451069_at Pim3 BC017621 1.09 1.50 1450135_at Fzd3 AU043193 1.09 1.42 1420978_at Nrf1 NM_010938 1.09 1.45 1450384_at Bace1 AF200346 1.09 1.92 1455194_at Mapk8ip2 AW536912 1.09 1.57 1419283_s_at Tns1 NM_027884 1.09 1.58 1445415_at EG328191 AW048415 1.09 0.70 1423449_a_at Actn4 BM234779 1.09 1.46 1449556_at H2-T23 NM_010398 1.09 1.63 1442094_at Ing5 BB470038 1.08 0.71 1426434_at Tmem43 AI747289 1.08 1.68 1434448_at Txlna BG070463 1.08 1.47 1438459_x_at Sfpq BF224766 1.08 1.43 1421342_at Kcns2 NM_008436 1.08 1.45 1426441_at Slc11a2 BG065264 1.08 1.44 1447326_s_at Zmym3 AW122925 1.08 1.55 1450399_at Psen1 L42177 1.08 1.74 1428352_at Arrdc2 AW542672 1.08 1.41 1419385_a_at Ubqln1 NM_026842 1.08 1.46 1436789_at Ccnjl BM120177 1.08 1.56 1417694_at Gab1 NM_021356 1.08 1.50 1435390_at Exod1 BG067990 1.08 1.49 1456570_at Epb4.1l4b BB801576 1.08 1.66 1452197_at Smc4 AV172948 1.08 1.70 1444468_at Paqr8 AV328983 1.07 1.66 1449026_at Ifnar1 NM_010508 1.07 1.57 1421016_at Ighmbp2 AW259474 1.07 1.41 1427579_at Rhbdl3 BE653576 1.07 1.58 1416783_at Tac1 NM_009311 1.07 1.56 1451446_at Antxr1 AF378762 1.07 1.60 1436787_x_at Sec14l3 AV024133 1.07 0.53 1448235_s_at Hmgb1 BF166000 1.07 0.66 1455763_at Rnf41 BB753958 1.07 0.66 1452524_a_at Elavl3 U29149 1.07 1.51 1436353_at A230046K03Rik AU018634 1.06 1.50 1458584_at 4832406H04Rik BE948494 1.06 1.56 1423678_at BC017643 BC017643 1.06 1.59 1432029_a_at Smap1 AK006817 1.06 1.54 1448591_at Ctss NM_021281 1.06 1.81 1437864_at Adipor2 BE632137 1.06 1.72 1426466_s_at Rps6kl1 BB311088 1.06 1.49 1431422_a_at Dusp14 AK009744 1.06 1.74 1455913_x_at Ttr AV152953 1.06 0.14 1442823_at BB536648 1.05 1.79 1424613_at Gprc5b BC020004 1.05 1.59 1442073_at BQ266693 1.05 1.67 1452441_at Phf3 AW910425 1.05 0.68 1422902_s_at Mgea5 NM_023799 1.05 1.47 1450748_at Smpd3 NM_021491 1.05 1.42 1426590_at Gfm2 BB497484 1.05 1.48 1442257_at Cdh6 BI134319 1.05 1.70 1431056_a_at Lpl AK017272 1.04 1.43 1417749_a_at Tjp1 NM_009386 1.04 1.53 1460694_s_at Svil BM203457 1.04 1.76 1426368_at Rin2 AK014548 1.04 1.47 1450875_at Gpr37 BQ175510 1.04 1.68 1419115_at Alg14 AA198774 1.04 0.61 1427189_at Arih1 BB362210 1.04 1.46 1435621_at Mlstd1 BQ175346 1.04 1.45 1437105_at Jarid1a BM246184 1.04 1.45 1450108_at Kif1a NM_008440 1.04 1.51 1454844_at Mchr1 AW049955 1.04 1.47 1418754_at Adcy8 NM_009623 1.04 1.41 1451115_at Pias3 BC023128 1.04 0.61 1419058_at Polr1e NM_022811 1.04 1.56 1432452_at 1700054O19Rik AK006789 1.04 0.70 1435444_at Atf6 AV270913 1.04 1.42 1431717_at 3526401B18Rik AK014386 1.03 1.63 1452295_at Tmepai AV291712 1.03 1.46 1431035_at Daam1 AW988556 1.03 1.67 1434791_at Atp6v0a2 BM229554 1.03 1.50 1459703_at D7Ertd443e BG067408 1.03 0.55 1422688_a_at Nras BB018528 1.03 1.61 1424532_at Ylpm1 AB033168 1.03 1.60 1448439_at D17Wsu104e NM_080837 1.03 1.59 1416541_at Clpb NM_009191 1.03 1.43 1434548_at Serinc3 BM244064 1.03 1.44 1435590_at D430047L21Rik AV325177 1.03 1.47 1425803_a_at Mbd2 AF072245 1.03 1.41 1437788_at Sp6 BB473680 1.03 0.55 1455945_at Zfp87 AV282347 1.03 1.87 1441323_at Qpctl AV370587 1.03 1.61 1417356_at Peg3 AB003040 1.02 1.44 1424111_at Igf2r BG092290 1.02 1.66 1433599_at Baz1a BM239162 1.02 3.59 1416550_at Slc35b4 BB320416 1.02 1.70 1434506_at Arid2 AV273623 1.02 1.43 1450850_at Vil2 BM119387 1.02 1.64 1428623_at Plxna1 AK011193 1.02 1.43 1450213_at Pde7b NM_013875 1.02 1.46 1454995_at Ddah1 AW556888 1.02 1.57 1420011_s_at Xbp1 C77390 1.02 1.48 1435295_at Dopey1 BG075614 1.01 1.42 1437467_at Alcam AV315205 1.01 0.71 1454680_at D5Ertd579e BG073020 1.01 1.41 1420619_a_at Aes NM_010347 1.01 0.70 1452690_at Khsrp BQ174458 1.01 0.58 1460733_at AA407659 BB390422 1.01 1.41 1417174_at 1810021J13Rik NM_025464 1.01 1.42 1459544_at BB180990 1.01 1.44 1438638_x_at 1700027J05Rik BB195788 1.01 0.51 1438887_a_at Gmcl1 BM239632 1.01 1.64 1423531_a_at Hnrpa1 BI663320 1.01 1.71 1434143_at BC060631 BQ266886 1.01 1.42 1435580_at C230081A13Rik AW553275 1.01 1.46 1420984_at Pctp AF114437 1.00 0.50 1431080_at 1700021K10Rik BB611374 1.00 1.51 1437626_at Zfp36l2 BB031791 1.00 1.43 1427135_at Sfrs12 AV012790 1.00 0.49 1454845_x_at Mchr1 AW049955 1.00 1.43 1424471_at Rapgef3 BC020532 1.00 1.62 1433776_at Lhfp AV149705 1.00 1.73 1417566_at Abhd5 AK007421 1.00 1.46 1432435_s_at C030004A17Rik AK012261 1.00 1.47 1432161_a_at Ptar1 AK006988 1.00 0.63 1448954_at Nrip3 NM_020610 1.00 1.53 1416620_at Smarcal1 NM_018817 0.99 1.43 1425844_a_at Rngtt AF034568 0.99 1.67 1437858_at Dpy19l3 AV367203 0.99 1.51 1451191_at Crabp2 BC018397 0.99 1.58 1450407_a_at Anp32a AF022957 0.99 0.63 1416527_at Rab32 NM_026405 0.99 1.44 1426712_at Slc6a15 BB129409 0.99 1.40 1415836_at Aldh18a1 NM_019698 0.99 1.56 1423301_at Copb1 BF147382 0.99 1.46 1421471_at Npy1r NM_010934 0.99 1.56 1435171_at Tbl1xr1 BB667085 0.99 1.50 1417153_at Btbd14a NM_026495 0.99 0.63 1420922_at Usp9x AW107303 0.99 1.45 1441300_at Kcnf1 BB275623 0.98 1.52 1452415_at Actn1 BC003232 0.98 0.67 1455980_a_at Gas2l3 BB770972 0.98 1.41 1460735_at Svil BM203457 0.98 1.77 1424290_at Osgin2 BB817847 0.98 1.75 1443793_x_at Ints1 BB444598 0.98 2.51 1453264_at Marveld3 AK007346 0.98 0.71 1416432_at Pfkfb3 NM_133232 0.98 1.54 1422469_at Tbk1 NM_019786 0.98 1.41 1423419_at Lig3 BM248530 0.98 1.48 1424876_s_at Spg20 BB040507 0.98 1.43 1429684_at 5830472M02Rik BG094398 0.98 2.11 1439163_at Zbtb16 BQ174973 0.98 1.44 1451142_at Exoc1 BC024678 0.97 1.43 1439909_at Zfp521 BE133660 0.97 1.47 1454944_at Hic2 BE648070 0.97 1.46 1423569_at Gatm AW108522 0.97 1.52 1438511_a_at 1190002H23Rik BB408123 0.97 1.56 1455214_at Mitf BB763517 0.97 1.53 1425007_at Zfp566 BC019790 0.97 1.50 1454647_at Acad11 BQ031255 0.97 1.45 1453527_a_at Neurl AK010787 0.97 0.65 1421844_at Il1rap BE285634 0.97 1.53 1443773_at Ylpm1 AI851834 0.97 1.77 1433649_at Aof1 BM243793 0.96 1.41 1428560_at Xpo5 AK012248 0.96 1.67 1431066_at Fut11 BB626220 0.96 1.61 1423311_s_at Tpbg BQ177165 0.96 1.56 1440251_s_at Zfp64 BE947768 0.96 1.45 1437244_at Gas2l3 BB770972 0.96 1.79 1437745_at Chd7 BB127276 0.96 1.45 1418937_at Dio2 AF177196 0.96 1.53 1437247_at Fosl2 BM245170 0.96 1.43 1456233_at BB080923 0.96 1.46 1426733_at Itpk1 AW552407 0.96 1.56 1452312_at 2810002D19Rik BC022909 0.96 1.75 1454070_a_at Ddhd1 AK014975 0.96 1.71 1422502_at Parp1 AF126717 0.96 1.50 1425987_a_at Kcnma1 L16912 0.96 1.43 1434737_at Obfc1 AV000765 0.95 1.44 1425529_s_at D19Wsu162e BC026369 0.95 1.48 1415685_at Mtif2 NM_133767 0.95 1.52 1420669_at Arnt2 AI428888 0.95 1.49 1452292_at Ap2b1 AV271093 0.95 1.44 1442754_at C030013G03Rik BE692283 0.95 2.35 1420003_at Nsun5 C80273 0.95 1.48 1426979_at Mlxip AF265663 0.95 1.42 1423247_at Txndc4 BI100077 0.94 1.52 1419669_at Prtn3 U97073 0.94 0.64 1420886_a_at Xbp1 NM_013842 0.94 1.48 1416505_at Nr4a1 NM_010444 0.94 0.65 1426606_at Crtac1 BB426194 0.94 1.78 1434537_at Slco3a1 BM237089 0.94 1.43 1428273_at Abhd13 BE573480 0.93 1.53 1427071_at Fbxo42 BC003960 0.93 1.44 1435309_at Rbm13 AV030795 0.93 1.80 1420852_a_at B3gnt2 AV306734 0.93 1.49 1438236_at Nfia BB485864 0.93 0.64 1437818_at 9430016H08Rik AV228487 0.93 1.43 1452963_at 9530077C05Rik AK003384 0.92 1.62 1455735_at Ap1s3 AW259574 0.92 1.45 1427510_at Gnai1 U38501 0.92 1.56 1417878_at E2f1 NM_007891 0.92 0.65 1428174_x_at Khsrp BQ174458 0.92 0.62 1426677_at Flna BM233746 0.92 1.45 1428803_at Acot6 AK014558 0.92 1.43 1418786_at Mapk8ip2 AF220195 0.92 1.52 1434023_at Ccdc100 BG065282 0.92 1.41 1437183_at Lrrc4b AW491122 0.92 1.49 1454730_at Tapt1 AV273867 0.92 1.41 1425287_at Zfp189 BC021326 0.92 1.40 1419224_at Cecr6 NM_033567 0.91 1.40 1425111_at Sorcs3 AK018111 0.91 1.69 1451833_a_at Setdb1 BC007176 0.91 1.56 1418969_at Skp2 AV259620 0.91 2.19 1453727_at Esfl BB612598 0.91 1.89 1454989_at Cspp1 AW553463 0.91 1.68 1450679_at 5730470L24Rik BC004090 0.91 1.50 1451033_a_at Trpc4 BB271442 0.91 1.41 1427998_at Lsm12 AV015526 0.91 1.46 1428911_at Ttll4 AV231607 0.91 1.49 1443955_at 4933434E20Rik BG074246 0.91 0.71 1427208_at Zfp451 BC024435 0.91 1.48 1424686_at 2700008B19Rik BF150550 0.90 1.55 1460737_at Igbp1 AA960310 0.90 1.83 1418726_a_at Tnnt2 NM_011619 0.90 0.67 1435214_at Gja12 BB319817 0.90 1.54 1424755_at Hip1 BB320674 0.90 0.68 1451734_a_at Dbn1 AB064321 0.90 1.41 1416389_a_at Rcbtb2 NM_134083 0.90 1.59 1426835_at Metap1 BG064851 0.90 1.41 1421024_at Agpat1 BB524140 0.89 0.69 1420287_at R75193 0.89 1.41 1418839_at Glmn NM_133248 0.89 1.42 1421771_a_at Ipp NM_008389 0.89 1.51 1416348_at Men1 AB023401 0.89 1.73 1458349_s_at BB278163 0.89 0.64 1434815_a_at Mapkapk3 AI451985 0.89 1.57 1453742_at Vps33a BG087455 0.89 1.55 1421835_at Mtap7 NM_008635 0.89 1.49 1435841_s_at Suclg2 BB757840 0.89 1.42 1427088_at Ccnt2 BI872151 0.88 1.70 1428750_at Cdc42ep2 BF453885 0.88 1.82 1431939_a_at Mina AK014426 0.88 1.40 1452699_at Mett11d1 AK009576 0.88 1.65 1429249_at 4833424O15Rik BB346520 0.88 1.71 1428757_at Aasdhppt AK013111 0.88 1.52 1433618_at C330006A16Rik BB397899 0.88 0.69 1438114_x_at Efs BB168690 0.88 1.50 1419400_at Mttp AW553649 0.87 1.42 1424842_a_at Arhgap24 BC025502 0.87 1.43 1433820_a_at 1110012D08Rik BB489180 0.87 1.53 1438115_a_at Slc9a3r1 BB805362 0.87 1.65 1424894_at Rab13 BC027214 0.87 0.66 1421235_s_at Recql5 NM_130454 0.86 1.47 1439623_at Otud1 BE687142 0.86 0.71 1426563_at Zfp553 BB770954 0.86 1.47 1451136_a_at Eif2b2 BC003326 0.86 1.42 1421895_at Eif2s3x NM_012010 0.86 1.83 1435076_at 2310047D13Rik AV291303 0.85 1.53 1418145_at Tfip11 NM_018783 0.85 1.50 1448346_at Cfl1 NM_007687 0.85 0.71 1441867_x_at 4930534B04Rik AI480494 0.85 0.65 1445763_at 1700013F07Rik BG069737 0.85 0.63 1445824_at Zfp458 AV276001 0.84 1.43 1423560_at Nell2 AI838010 0.84 1.68 1436185_at AI314180 BG076313 0.84 1.50 1452324_at Pvt1 BE956863 0.84 0.70 1435005_at Cenpe BG916502 0.84 2.25 1435937_at Sptlc2 BB794710 0.84 1.55 1444020_at Ncan BM939365 0.84 2.00 1448614_at Nt5c2 BI202534 0.84 1.51 1444874_at Atp5g1 BG074583 0.84 1.52 1460602_at Dlc1 BB768194 0.84 0.61 1416521_at Sepw1 NM_009156 0.84 0.56 1418526_at Fusip1 NM_010178 0.83 1.48 1454745_at Arhgap29 BG074320 0.83 0.70 1417528_at Spag6 NM_015773 0.83 1.43 1459896_at Pogk AW911766 0.83 1.49 1426546_at Tesk2 BQ179435 0.83 1.66 1431469_a_at Cxxc5 AK015150 0.83 0.71 1449365_at Edg8 NM_053190 0.83 1.90 1426217_at 2810441K11Rik AF332866 0.82 1.74 1431131_s_at A630007B06Rik BI106826 0.82 1.41 1439844_at 8430426J06Rik BE628126 0.82 0.70 1437122_at Bcl2 BM119782 0.82 1.52 1456026_at 8030451K01Rik AV303159 0.82 1.83 1418107_at Tcea2 NM_009326 0.82 1.46 1421137_a_at Pkib AV047342 0.82 2.01 1419621_at Ankrd2 NM_020033 0.82 2.02 1454727_at Afap111 BB106834 0.82 1.69 1444395_at Dixdc1 AW455436 0.82 1.77 1448942_at Gng11 NM_025331 0.82 1.54 1417400_at Rai14 NM_030690 0.81 1.74 1416714_at Irf8 BG069095 0.81 1.82 1430135_at Dnase2a AK018651 0.81 0.70 1427148_at Pja2 BF160731 0.81 1.44 1438517_at Wwox BB818997 0.81 1.54 1428919_at Fgfr1op BB667817 0.81 1.47 1460623_at Skap2 BB753881 0.81 0.70 1417384_at Entpd5 NM_007647 0.81 1.61 1422910_s_at Smc6 AU022584 0.80 1.58 1460136_at AW047481 AI462839 0.80 1.42 1423447_at Clpx BF020441 0.80 1.57 1441055_at Akap2 BB431157 0.80 0.60 1434981_at E130303B06Rik AA116393 0.80 1.57 1431592_a_at Sh3kbp1 AK007283 0.79 1.56 1421017_at Nrg3 NM_008734 0.79 1.53 1460390_at Sorl1 BI648081 0.79 1.53 1423339_s_at Ccdc16 AI530149 0.79 1.49 1457816_at Casp9 BB781510 0.79 0.63 1447359_at Zfp575 AI326876 0.78 0.71 1417637_a_at Hmg20b NM_010440 0.78 0.61 1422472_at Pex13 BB045429 0.78 1.45 1449214_a_at Opa1 BC025160 0.78 1.70 1443863_at Fndc3a AA770704 0.78 0.64 1418417_at Msc NM_010827 0.78 1.60 1429162_at 1500015A07Rik AK005240 0.78 2.31 1452884_at Sfrs2ip AK012092 0.78 1.64 1436020_at D8Ertd457e AV099356 0.77 1.59 1458249_at BB771227 0.77 1.41 1424687_at 2700008B19Rik BF150550 0.77 1.47 1447228_at 1110051M20Rik BM248411 0.77 1.41 1456859_at Lnp BI133590 0.77 1.65 1428328_at Nup50 AK006556 0.76 1.41 1434089_at Synpo BB426294 0.76 1.72 1458103_at Ncor1 AV325208 0.76 0.69 1436326_at Rora BB306272 0.75 0.65 1419309_at Pdpn NM_010329 0.75 1.50 1439884_at Nudt16 AI851783 0.75 0.70 1442707_at Camk2a BI134616 0.75 0.68 1427982_s_at Syne2 BF582734 0.74 1.59 1429758_at 1700017B05Rik BB046208 0.73 0.69 1435119_at BE956710 0.73 1.59 1445421_at AV238942 0.73 1.84 1432187_at Nup43 AK011422 0.73 1.44 1453105_at Zfp263 BG069835 0.72 1.48 1459472_at AU022583 AU022583 0.72 1.83 1457426_at 1700048O20Rik BE980678 0.72 0.69 1422557_s_at Mt1 NM_013602 0.71 0.88 1423732_at Tram1 BC012401 0.71 0.90 1428281_at Trub1 AK011362 0.71 0.97 1440795_x_at Rabep2 BB259371 0.71 1.12 1417886_at Ints5 BE307471 0.71 0.91 1429232_at 2610528B01Rik AK012160 0.71 1.09 1436393_a_at Trim37 BG065227 0.71 1.03 1438278_a_at BC003993 BB532946 0.71 1.07 1440161_at Mmp16 BB378819 0.71 1.37 1442408_at 2010305B15Rik BE981170 0.71 1.05 1453783_at Pura BE864772 0.71 0.93 1421375_a_at S100a6 NM_011313 0.71 0.96 1424942_a_at Myc BC006728 0.71 0.88 1426265_x_at Dlat AV336908 0.71 0.94 1426583_at Atf2 BM119623 0.71 1.15 1427104_at Zfp612 BG076162 0.71 0.99 1429790_at Brunol6 AK018246 0.71 0.93 1438684_at Nuak1 BF021001 0.71 1.57 1452127_a_at Ptpn13 BM236743 0.71 1.01 1420767_at 1700019G17Rik NM_029331 0.71 0.88 1433628_at Coq10a BQ044689 0.71 1.03 1440512_at AW048066 BF471100 0.71 0.91 1444139_at Ddit41 BG797099 0.71 0.80 1446244_at Zyg11b BB077439 0.71 0.94 1452609_at Cox4nb BI133691 0.71 0.80 1423757_x_at Igfbp4 BC019836 0.71 0.83 1424430_at Mterfd2 BC002280 0.71 1.22 1426289_at Qrich1 BC006738 0.71 0.93 1434301_at D330050I23Rik BE303700 0.71 0.82 1460362_at 2410001C21Rik BC004803 0.71 0.98 AFFX-BioC- AFFX- 0.71 0.86 5_at BIOC-5 1419101_at Sin3a NM_011378 0.71 1.39 1419606_a_at Tnnt1 NM_011618 0.71 0.96 1429601_x_at 1110019K23Rik AK003824 0.71 1.37 1433500_at Dennd2a AV260555 0.71 1.17 1458163_at BC066028 AV328953 0.71 0.93 1437623_x_at Xrcc3 BB251723 0.71 0.77 1449307_at Dbndd1 NM_028146 0.71 0.88 1450448_at Stc1 BQ032752 0.71 1.02 1459804_at Crebbp BB475090 0.71 1.27 1435077_at Asxl1 BE956516 0.71 0.87 1445461_at BB096245 0.71 1.08 1448495_at Tsta3 NM_031201 0.71 1.01 1448923_at Prkra NM_011871 0.71 0.79 1456061_at Gimap8 BM243674 0.71 0.91 1457262_at 2610207I05Rik BB024162 0.71 1.32 1417204_at Kdelr2 AJ278133 0.71 1.12 1419789_at Centg1 AI605509 0.71 2.20 1428903_at 3110037I16Rik AK014134 0.71 0.95 1449935_a_at Dnaja3 AK004575 0.71 1.13 1433639_at 5730593F17Rik AW548096 0.70 0.72 1436388_a_at 3830406C13Rik BB763097 0.70 0.93 1436581_at Ccdc39 BQ174391 0.70 1.18 1436875_at Dnm3 BB130476 0.70 1.12 1453343_s_at Vrk2 AK012664 0.70 1.08 1424228_at Polr3h AK019868 0.70 0.93 1426138_a_at Ube2j2 AF296656 0.70 1.13 1432972_at 4921518B13Rik AK014916 0.70 1.51 1436833_x_at Ttl11 BB251824 0.70 0.94 1452721_a_at Ccdc53 BM210204 0.70 0.96 1418236_s_at Atg5 AV168389 0.70 1.15 1427098_at Wwp1 BI102531 0.70 0.85 1429243_at 1110054O05Rik BG076073 0.70 0.95 1431059_x_at Htatsf1 AK012522 0.70 1.01 1436099_at AI836003 AV294537 0.70 0.90 1437591_a_at Wdr1 AW542545 0.70 0.99 1452880_at Znhit3 AK003721 0.70 0.96 1425580_a_at Pik3c3 BC024675 0.70 1.24 1426624_a_at Ypel3 BI660196 0.70 0.89 1429710_at Styx BB812465 0.70 1.24 1436456_at 9130023D20Rik BQ031896 0.70 1.34 1438028_at 4930535B03Rik BB817800 0.70 1.13 1460609_at Ints8 BB224305 0.70 0.78 1426078_a_at Gpr108 AF376726 0.70 1.02 1440895_at LOC665193 BB122340 0.70 0.98 AFFX-r2-Ec- “AFFX-R2- bioC-3_at EC-BIOC-3” 0.70 0.96 1418970_a_at Bcl10 AF100339 0.70 1.02 1419257_at Tcea1 BC006022 0.70 0.87 1422857_at Trip4 AV350958 0.70 1.02 1423191_at Fnbp4 AK013152 0.70 1.18 1435864_a_at 1810063B05Rik BG975168 0.70 0.89 1438269_at Zbtb38 BB278987 0.70 0.73 1456366_at 1700015F17Rik BB833901 0.70 0.82 1460670_at Riok3 NM_024182 0.70 1.00 1425203_at Ddx19b BE553267 0.70 0.79 1428172_at Prpf39 BB460975 0.70 1.21 1431429_a_at Arl4a AK006286 0.70 1.11 1436617_at Cetn4 AV211098 0.70 0.92 1437449_at Rsad1 BB818348 0.70 0.95 1456884_at LOC67660 AI854003 0.70 0.95 1419655_at Tle3 NM_009389 0.70 1.40 1430694_at Star BE630656 0.70 0.94 1435744_at 6720401G13Rik BG075556 0.70 0.94 1437202_at Mysm1 BB494594 0.70 1.00 1449746_s_at Krr1 AU020154 0.70 0.90 1423144_at Pik3ca AI528567 0.70 1.13 1426879_at 1190005F20Rik AK004493 0.70 1.17 1428285_at 8430427H17Rik AK018446 0.70 1.05 1443870_at Abcc4 BB291885 0.70 1.17 1452445_at Slc41a2 BC026874 0.70 1.02 1460434_at Fundc2 AI510221 0.70 0.88 1423363_at Sort1 AV247637 0.69 0.80 1436373_at Map3k10 AA789425 0.69 0.97 1437606_at BB829614 0.69 0.89 1448222_x_at Cox8a NM_007750 0.69 0.97 1449999_a_at Cacna2d1 NM_009784 0.69 1.01 1460639_a_at Atox1 NM_009720 0.69 0.96 1419046_at Brp16 NM_021555 0.69 1.25 1421204_a_at Nudt16 NM_029385 0.69 0.94 1422622_at Nos3 NM_008713 0.69 1.16 1439332_at Ddit41 AV251625 0.69 0.92 1455661_at BB211563 0.69 0.94 1429229_s_at 4930534B04Rik BE980134 0.69 0.77 1450849_at Hnrpu BF228203 0.69 2.02 1430999_a_at Scoc BM932452 0.69 0.96 1433969_at AU067824 AU067824 0.69 1.01 1460005_at AF013969 BB542960 0.69 0.95 1435027_at Golga1 AW552851 0.69 1.09 1439341_at AK220484 BB203252 0.69 1.12 1450881_s_at Gpr137b AK010724 0.69 1.02 1417065_at Egr1 NM_007913 0.69 0.99 1424922_a_at Brd4 BC008532 0.69 0.99 1427390_at Bloc1s3 BC025913 0.69 0.94 1450083_at Cnot4 AI448404 0.69 0.97 1458464_at Hecw2 BB445169 0.69 1.22 1453381_at C030033M12Rik BB771017 0.69 1.04 1427992_a_at Rab12 BF729629 0.69 1.01 1419130_at Deadc1 NM_025748 0.69 1.94 1428600_at Nin AK014241 0.69 0.99 1431820_at 4632404H12Rik AK014549 0.69 0.83 1447827_x_at Spop AV353779 0.69 1.15 1420579_s_at Cftr NM_021050 0.68 2.52 1429058_at 1110004B13Rik AK003403 0.68 0.84 1435237_at 2310009A05Rik AV004950 0.68 0.81 1453684_s_at Zc3h15 AK005661 0.68 1.03 1454968_at 1110034A24Rik AV011961 0.68 1.00 1418135_at Aff1 NM_133919 0.68 1.10 1418971_x_at Bcl10 AF100339 0.68 0.99 1437003_at BB323930 0.68 0.71 1448306_at Nfkbia NM_010907 0.68 1.13 1416607_at 4931406C07Rik AI461712 0.68 1.13 1431120_a_at Golga1 BC024637 0.68 0.97 1435544_at Exosc6 BB446614 0.68 1.10 1439888_at BB271581 0.68 1.17 1444150_at Epb4.1 BB462549 0.68 0.88 1448218_s_at Ywhaz AV027921 0.68 0.86 1457825_x_at Tcn2 BB515151 0.68 1.25 AFFX-r2-Ec- “AFFX-R2- bioC-5_at EC-BIOC-5” 0.68 0.89 1418432_at Cab39 AK005226 0.68 1.39 1426230_at Sphk2 AK016616 0.68 0.81 1433743_at Dach1 BG075820 0.68 0.82 1437203_at Cbll1 BB460904 0.68 1.17 1458951_at Vrk1 AV341598 0.68 1.07 1421087_at Per3 NM_011067 0.68 0.95 1455074_at Efcab1 AV307860 0.68 1.31 AFFX-r2-Ec- “AFFX-R2- bioB-3_at EC-BIOB-3” 0.68 0.86 1420053_at Psmb1 C81484 0.68 0.93 1421097_at Endog NM_007931 0.68 0.97 1426326_at Zfp91 U05343 0.68 0.99 1434423_at Gulp1 BB138485 0.68 0.83 1437866_at Dusp18 AV276425 0.68 1.26 1418024_at Narg1 BG067031 0.68 1.14 1430168_at Cstad AK020589 0.68 0.99 1433720_s_at Ndg2 AI647775 0.68 0.95 1440060_at EG331480 AV306340 0.68 1.03 1453754_at S100pbp AK015229 0.68 0.76 1457232_at Fbxl21 BE946365 0.68 0.93 1436104_a_at 2310015A05Rik BG068839 0.68 0.93 1439780_at Rpl711 BG071958 0.67 0.56 1451270_at Dusp18 BC020036 0.67 1.11 1453993_a_at Bnip2 AK014659 0.67 0.88 1428635_at Comtd1 AK007659 0.67 0.97 1433793_s_at Nrip2 AW491344 0.67 0.97 1439497_at 4933415E08Rik BG065013 0.67 0.68 1455463_at Phyhip BB427286 0.67 0.76 1419193_a_at Gmfg NM_022024 0.67 1.00 1447641_at Dmwd AV354897 0.67 0.91 1457354_at Krt222 AI845957 0.67 0.83 1458425_at My16b BM945176 0.67 1.04 1420530_at Neud4 AW553317 0.67 0.80 1423456_at Bzw2 BM932775 0.67 1.04 1423477_at Zic1 BB361162 0.67 1.10 1429914_at Epc1 AK017858 0.67 1.03 1439699_at Pgr BB525237 0.67 0.84 1443579_s_at Depdc6 AI957118 0.67 0.96 1430222_at 9130007G19Rik BB538672 0.67 0.86 1454086_a_at Lmo2 AK013416 0.67 1.01 1428659_at Phf7 AK005852 0.67 0.67 1440202_at 5930412I24Rik BB036159 0.67 1.60 1440338_at E430028B21Rik BM933532 0.67 0.98 1417387_at Med31 NM_026068 0.67 0.98 1423630_at Cygb BM899392 0.67 0.92 1424486_a_at Txnrd1 BB284199 0.67 0.84 1434145_s_at Serhl C80355 0.67 1.12 1435365_at 4732415M23Rik BB614874 0.67 1.07 1429842_at Mdh1b AK007262 0.67 1.04 1436796_at 1110061A14Rik BB237072 0.67 1.15 1451751_at Ddit41 AF335325 0.67 0.95 1452849_at Sin3b AK013202 0.67 0.87 1418408_at Zfand1 NM_025512 0.67 1.04 1430286_s_at 4930402H24Rik AK013448 0.67 0.93 1437840_s_at LOC673662 BB222646 0.67 1.05 1444001_at Strbp AW488249 0.67 1.51 1457669_x_at Rfc2 AV096765 0.67 0.96 1432378_at C030004G16Rik AK021043 0.66 1.03 1451132_at Pbxip1 AV220340 0.66 1.01 1417107_at Tpd5212 NM_025482 0.66 0.94 1421997_s_at Itga3 NM_013565 0.66 1.11 1448851_a_at Dnajc5 AK012029 0.66 1.11 1451213_at Pex11b BB010355 0.66 1.07 1456645_at Wdr25 BB352668 0.66 0.72 1422842_at Xrn2 NM_011917 0.66 1.04 1452517_at Plekhh1 BC006045 0.66 1.25 1424169_at Tax1bp3 BC008166 0.66 0.73 1424207_at Smarca5 BI661719 0.66 1.36 1428841_at Best1 AK006549 0.66 0.99 1425528_at Prrx1 L06502 0.66 1.16 1451020_at Gsk3b BB831420 0.66 0.90 1452228_at Tbc1d23 AV209678 0.66 0.94 1419144_at Cd163 NM_053094 0.66 0.96 1441742_at Kcnh5 AW048994 0.66 1.08 1448553_at Myh7 NM_080728 0.66 0.86 1416622_at Wbscr16 NM_033572 0.66 1.37 1423834_s_at Gga1 BC026802 0.66 0.92 1449470_at Dlx1 NM_010053 0.66 0.87 1426360_at Zc3h11a AV328883 0.66 1.33 1419266_at Nfyb AV250496 0.65 1.22 1432391_at Ccdc21 AK010621 0.65 0.84 1441453_at Dennd2a BB341122 0.65 0.94 1455801_x_at Tbcd BB392080 0.65 1.03 1429125_at Zbtb9 AV283825 0.65 0.93 1426584_a_at Sord BI143942 0.65 0.85 AFFX-BioDn- “AFFX- 3_at BIODN-3” 0.65 0.90 1416112_at Cox8a NM_007750 0.65 0.96 1418172_at Hebp1 AF117613 0.65 1.21 1427334_s_at 2810474O19Rik BE196832 0.65 0.95 1429362_a_at Sf3b2 BF022081 0.65 1.01 1453006_at Fgfbp3 AI506568 0.65 1.00 1424899_at Nmnat3 BC005737 0.65 0.95 1437018_at Pnma2 BG072348 0.65 1.16 1422164_at Pou3f4 X66603 0.65 0.99 1452817_at Smyd3 AK010447 0.65 0.99 1437118_at Usp7 C77542 0.65 1.76 1460021_at EG626231 BB825323 0.65 1.28 1422662_at Lgals8 AI987967 0.64 0.94 1428198_at Adal AK016299 0.64 1.10 1423184_at Itsn2 AI326108 0.64 0.76 1426631_at Pus7 BM199125 0.64 1.11 1437694_at Zfp809 AW536892 0.64 0.92 1455586_at Rnf168 BB275228 0.64 0.49 1447494_at D7Bwg0826e R74675 0.64 1.01 1430242_at Rwdd1 AV356665 0.64 1.14 1430745_at 5930409G06Rik AK020028 0.64 0.71 1458685_at Garnl1 BB552367 0.64 1.03 1438477_a_at Mcee AA960125 0.64 0.86 1446357_at BC020402 BB029175 0.64 1.47 1447097_at 8430426H19Rik BG068083 0.64 0.88 1438491_x_at A530054K11Rik BF227108 0.63 1.22 1448547_at Rassf3 BB703307 0.63 0.85 1450543_at Kctd10 NM_026145 0.63 0.93 1423284_at Mansc1 AK002644 0.63 0.99 1441316_at LOC677060 BB234335 0.63 0.59 1452594_at Dusp11 BB004080 0.63 1.19 1428943_at Nudt13 BE134119 0.63 1.05 1438732_at 6430511F03 BE947483 0.63 1.04 1453771_at Gulp1 BF144687 0.63 0.92 1417219_s_at Tmsb10 NM_025284 0.63 0.67 1424487_x_at Txnrd1 BB284199 0.63 1.09 1426458_at Slmap BB473571 0.63 0.92 1448417_at Ninj1 NM_013610 0.63 0.90 1452161_at Tiparp BB707122 0.63 0.67 1427030_at Ccdc52 BG067674 0.63 0.83 1442281_at D4Ertd796e BG069783 0.63 0.86 1451677_at Narf BI452475 0.63 1.02 1429040_at 6820431F20Rik BE692399 0.63 1.33 1416661_at Eif3s10 AW701127 0.63 1.18 1421479_at Zfp318 NM_021346 0.62 0.78 1452187_at Rbm5 BB541007 0.62 1.32 1456120_at 3110001I20Rik BB427489 0.62 0.95 1428863_at Ankrd39 AK018681 0.62 1.00 1457038_at Frem2 BM201912 0.62 1.71 1457072_at Bcl11a BF731393 0.62 1.04 1419924_at Fnip1 AW557298 0.62 1.60 1425927_a_at Atf5 AF375476 0.62 1.00 1426832_at Ddx26b BB460424 0.62 1.08 1454777_at Slco2b1 BB553107 0.62 0.92 1418323_at Fem1b BM232562 0.61 1.13 1425309_at Catsper2 AF411816 0.61 0.98 1456517_at Tmem44 BB035891 0.61 0.92 1429260_at 1810014B01Rik AW985991 0.61 1.07 1445099_at BB383493 0.61 1.01 1452474_a_at Art3 AJ311773 0.61 1.07 1439073_at Mllt4 BB821719 0.61 1.20 1438481_at AW047464 AI854375 0.61 0.88 1442024_at Ppp1r3e BB328892 0.61 0.98 1453591_at 5730437N04Rik AK017626 0.61 1.06 1418894_s_at Pbx2 NM_017463 0.61 0.97 1440756_at BB712642 0.61 0.82 1456419_at 5730455P16Rik BI108098 0.61 0.87 1437305_at Zfp770 AV296864 0.60 0.98 1438928_x_at Ninj1 BB252065 0.60 0.82 1424117_at BC056474 BC024937 0.60 0.99 1454830_at Fbn2 AV010392 0.60 1.05 1458353_at Nwd1 BB173301 0.60 0.93 1427456_at Wdfy3 BF150771 0.60 1.09 1426421_s_at Rbm26 AK005802 0.60 1.39 1454174_a_at C330007P06Rik AK021190 0.60 2.16 1423353_at Crispld1 BE693025 0.60 0.90 1437108_at Lsm6 BE949068 0.60 0.88 1437884_at Arl5b BB545273 0.60 0.85 1447344_at AI447264 0.60 1.04 1450759_at Bmp6 NM_007556 0.60 1.22 1422230_s_at Cyp2a5 NM_007812 0.60 0.81 1428047_s_at Zfx BC028506 0.60 1.07 1432269_a_at Sh3kbp1 AK018032 0.59 1.49 1440215_at “OTTMUSG 00000004461” BG068916 0.59 0.93 1453083_at 6430701C03Rik AK018294 0.59 0.99 1455998_at Zc3h11a BM232738 0.59 0.96 1427924_at Stx17 BE943517 0.59 0.72 1449357_at 2310030G06Rik NM_025865 0.59 0.82 1438032_at Lrch1 BB755336 0.59 0.74 1417938_at Rad51ap1 BC003738 0.59 1.02 1453320_at 1700027A23Rik AK006407 0.59 1.02 1449109_at Socs2 NM_007706 0.58 1.03 1449677_s_at Tmem38b C77858 0.58 1.11 1452748_at Xrcc3 AW537713 0.58 1.06 1457693_a_at 6430537H07Rik AV343405 0.58 0.85 1441306_at 6820408C15Rik AW048149 0.57 0.97 1439783_at C330018D20Rik BB809870 0.57 0.79 1428826_at Nr6a1 AK007201 0.57 0.94 1456067_at Gli3 AW546010 0.57 0.87 1430520_at Cpne8 AW548480 0.57 1.29 1441161_at B230216G23Rik BB307791 0.57 2.63 1430240_a_at Clgn AK019534 0.56 1.04 1439555_at Rlf BB704706 0.56 1.30 1426534_a_at Arfgap3 BG067878 0.56 0.78 1445564_at BE688513 0.56 1.32 1456674_at E130016E03Rik BB772877 0.55 1.05 1435351_at 2310026E23Rik AA189197 0.55 1.25 1429984_at 5730455O13Rik AK017666 0.55 1.14 1447476_at Abcc10 BB079952 0.55 0.56 1419390_at Pde10a BQ180352 0.55 0.87 1442927_at Ptk2b BB548690 0.55 1.40 1418707_at Bag4 NM_026121 0.55 1.58 1457625_s_at Cdkl2 BE979937 0.55 1.12 1456333_a_at Arhgap6 BB367529 0.54 0.93 1442588_at EG329217 BB117375 0.53 0.80 1458438_at Ccdc122 AW554226 0.53 0.94 1441106_at Zfand5 BB320444 0.52 1.11 1447809_x_at BB080932 0.52 1.41 1452473_at Prr15 AJ132433 0.51 0.58 1453309_at 9330179D12Rik BB749938 0.51 0.70 1426478_at Rasa1 AA124924 0.51 1.36 1449669_at Rabif AW549708 0.51 0.91 1458397_at Dnaja1 BB260315 0.50 0.93 1449141_at Fblim1 BG070068 0.49 0.80 1444141_at Snx13 BB828008 0.49 1.95 1420533_at Gucy1a3 AK004815 0.49 0.92 1445165_at 1200003I07Rik BM194850 0.48 0.91 1460285_at Itga9 NM_133721 0.46 1.44 1426612_at Tipin AK011357 0.45 0.84 1441871_at 1810044D09Rik AV057045 0.45 1.12 1419230_at A830036E02Rik NM_010661 0.43 1.03 1441123_at ORF34 BB023801 0.43 1.41 1449388_at Thbs4 NM_011582 0.41 2.16 Probe = Affymetrix probe identification; Symbol = official gene symbol; FCH D1 = fold change in striatonigral (D1) cells; FCH D2 = fold change in striatopallidal (D2) cells.

TABLE 16 Gene translation changes upon chronic cocaine treatment Probe Symbol GenBank FCHD1 FCHD2 Probe Symbol GenBank FCHD1 FCHD2 1430648_at Scn2b AK013326 12.83 0.60 1446981_at A830010M20Rik BB127813 2.30 0.99 1455799_at Rorb BB751387 3.11 1.93 1435635_at Pcmtd1 BB549335 2.29 0.63 1445081_at A930041I02Rik BB335888 3.02 1.23 1424708_at Tmed10 BI409239 2.27 1.20 1452906_at Gtl2 BE990468 2.92 0.51 1448944_at Nrp1 AK011144 2.26 1.91 1448606_at Edg2 U70622 2.86 1.04 1434393_at Usp34 BM235696 2.23 1.35 1442061_at Btbd7 BB199960 2.60 1.60 1421090_at Epb4.1l1 NM_013510 2.21 1.10 1421978_at Gad2 AF326550 2.56 0.76 1460330_at Anxa3 AW702161 2.19 1.43 1426458_at Slmap BB473571 2.50 0.79 1429256_at Gtl2 AU067739 2.17 0.88 1432558_a_at Mal AK019046 2.50 0.82 1427464_s_at Hspa5 AJ002387 2.16 1.44 1433599_at Baz1a BM239162 2.48 2.03 1436898_at Sfpq B1738328 2.14 1.33 1417029_a_at Trim2 BB283676 2.45 1.80 1455607_at Rspo3 BG072958 2.12 0.97 1438388_at Nxph1 BB078029 2.45 0.77 1428306_at Ddit4 AK017926 2.10 0.26 1418189_sat Ramp2 AF146523 2.42 0.99 1440282_at Tulp4 BB268998 2.10 1.78 1429769_at Pggt1b BI107300 2.39 0.83 1454950_at B930006L02Rik BB699417 2.10 0.94 1457077_at BB306048 2.37 1.49 1417602_at Per2 AF035830 2.07 2.79 1438702_at Flrt2 BG075699 2.35 1.05 1421970_a_at Gria2 NM_013540 2.07 0.79 1418188_a_at Ramp2 AF146523 2.34 1.16 1454655_at Dgkd BB094429 2.07 1.58 1425693_at Braf M64429 2.32 1.10 1452507_at Dlx6 AF022078 2.04 0.57 1418937_at Dio2 AF177196 2.30 0.88 1419029_at Ero1l BM234652 2.04 1.07 1455182_at Kif1b AV104668 2.02 1.10 1437002_at C030011O14Rik BB303443 1.79 1.18 1450760_a_at Ing3 BB020556 2.01 0.61 1421189_at Gabrb3 BB367779 1.79 0.99 1417604_at Camk1 NM_133926 2.00 1.33 1420502_at Sat1 NM_009121 1.78 1.07 1418350_at Hbegf L07264 1.99 1.32 1433582_at 1190002N15Rik AV309085 1.78 1.29 1449372_at Dnajc3 BE624323 1.99 0.88 1423363_at Sort1 AV247637 1.78 1.17 1446147_at BB436856 1.98 0.69 1417875_at Ddx50 NM_053183 1.78 0.93 1429095_at Cenpp AK006234 1.97 0.84 1434673_at Gpr22 BB429977 1.78 0.53 1428157_at Gng2 AV021455 1.97 1.02 1424028_at 5830457O10Rik BC023107 1.78 0.49 1455831_at Fus BE985138 1.97 0.70 1421116_a_at Rtn4 NM_024226 1.77 0.78 1424186_at Ccdc80 BG074158 1.95 1.24 1428484_at Osbpl3 AK004768 1.77 1.73 1428112_at Armet AK014338 1.95 1.53 1426951_at Crim1 AK018666 1.77 1.15 1417110_at Man1a BB070019 1.95 1.64 1437857_at Dpy19l3 AV367203 1.77 1.51 1448545_at Sdc2 AU021035 1.94 0.98 1420850_at Crnkl1 AV143435 1.76 0.80 1452089_at Cacnb4 AW106929 1.94 0.88 1423066_at Dnmt3a BB795491 1.76 1.38 1450708_at Scg2 NM_009129 1.94 1.24 1448008_at Ankhd1 BG081523 1.76 1.31 1426340_at Slc1a3 BB357585 1.94 1.42 1421961_a_at Dnajb5 AI664344 1.76 1.31 1448115_at Htf9c NM_008307 1.93 0.97 1460705_at Rps6kb1 AI451506 1.76 0.85 1424486_a_at Txnrd1 BB284199 1.92 1.10 1428361_x_at Hba-a1 AK011116 1.76 1.22 1442695_at C030007I01Rik AW494399 1.91 1.41 1423720_a_at Sar1a BC005549 1.75 0.96 1418322_at Crem AI467599 1.91 1.84 1425130_a_at Ptpn5 U28216 1.75 0.87 1453796_a_at Ergic2 AK016275 1.90 0.69 1450208_a_at Elmo1 NM_080288 1.74 1.34 1437122_at Bcl2 BM119782 1.90 1.66 1438578_a_at Btbd10 BG064119 1.74 1.17 1420886_a_at Xbp1 NM_013842 1.90 1.27 1436661_at Dpp10 BQ174532 1.74 1.28 1420011_s_at Xbp1 C77390 1.89 1.19 1435471_at Zfp708 BE994129 1.74 0.57 1421354_at Prkg2 NM_008926 1.89 0.59 1453238_s_at A130040M12Rik AU018141 1.74 1.68 1443846_x_at Pank2 BB402666 1.88 0.39 1428946_at Ube1l2 BB417360 1.74 0.76 1428600_at Nin AK014241 1.88 1.04 1452377_at Mll1 AK017541 1.73 1.16 1422134_at Fosb NM_008036 1.88 1.16 1439434_x_at Pcmt1 BB317673 1.73 1.04 1449870_a_at Atp6v0a2 NM_011596 1.88 1.08 1438796_at Nr4a3 BE692107 1.73 1.85 1420720_at Nptx2 NM_016789 1.86 1.73 1426326_at Zfp91 U05343 1.73 1.14 1449552_at Zfr NM_011767 1.86 0.60 1419228_at Elac1 NM_028983 1.72 1.48 1452754_at Creld2 AK017880 1.86 1.41 1419647_a_at Ier3 NM_133662 1.72 1.08 1443579_s_at Depdc6 AI957118 1.84 0.61 1442887_at Gpr39 BQ175060 1.71 1.05 1434674_at Lyst BB463428 1.84 1.23 1456137_at Nrxn3 BB132137 1.71 0.68 1439780_at Rpl7l1 BG071958 1.84 0.81 1428834_at Dusp4 AK012530 1.71 1.84 1418380_at Terf1 NM_009352 1.84 0.92 1435520_at Msi2 BQ174527 1.71 1.58 1448943_at Nrp1 AK011144 1.83 1.16 1449164_at Cd68 BC021637 1.71 1.69 1455403_at Manea BB035197 1.83 0.97 1459750_s_at Gpr123 AU015577 1.71 1.25 1440371_at BQ176223 1.82 1.07 1436343_at Chd4 BM502696 1.70 1.37 1436387_at Homer1 BB398124 1.82 1.91 1420048_at C78859 C78859 1.70 0.94 1438458_a_at Sfpq BF224766 1.82 1.21 1456769_at Dusp3 BQ266434 1.70 1.37 1428130_at Lman1 BG071597 1.82 1.42 1451311_a_at Adipor1 BC014875 1.70 0.97 1449289_a_at B2m BF715219 1.82 1.50 1455085_at 1700086L19Rik BI526033 1.69 1.19 1433595_at Slc35d1 BB409668 1.82 1.34 1430869_a_at Habp4 BF460630 1.69 0.96 1417111_at Man1a BB070019 1.82 1.31 1427256_at Vcan BM251152 1.69 1.32 1437433_at B3galt2 BB254922 1.82 0.97 1437667_a_at Bach2 AW553304 1.68 2.26 1434913_at Hmgcll1 BF471959 1.81 0.72 1454920_at Uhrf2 BQ266387 1.68 1.28 1435770_at Txndc13 BB215068 1.81 1.03 1426736_at Gspt1 AB003502 1.68 1.10 1425942_a_at Gpm6b AF254879 1.81 0.99 1415986_at Clcn4-2 NM_011334 1.68 1.04 1416447_at Tmem30a BE986812 1.80 0.97 1437339_s_at Pcsk5 BB241731 1.68 1.48 1454158_at Mpp7 AK012883 1.80 1.19 1428967_at Igf1r BB446952 1.67 1.38 1416064_a_at Hspa5 NM_022310 1.80 1.54 1457142_at Efcbp1 BB667731 1.67 1.20 1417812_a_at Lamb3 NM_008484 1.80 0.95 1422886_a_at Clk4 NM_007714 1.67 0.66 1450029_s_at Itga9 NM_133721 1.79 1.14 1422579_at Hspe1 NM_008303 1.66 0.98 1429718_at Slitrk5 BB782729 1.79 1.11 1439333_at Kcnv1 BB078927 1.66 0.80 1448701_a_at Krit1 AF310134 1.66 1.12 1436051_at Myo5a BQ174518 1.58 0.95 1421900_at Eif2ak1 BB077436 1.66 0.85 1434421_at Islr2 BB344549 1.58 0.51 1452237_at Hrb BB130716 1.65 1.18 1430667_at Pcdh10 BB077413 1.58 1.16 1429159_at Itih5 AK018605 1.65 0.95 1435158_at Rbm12b BB796313 1.58 1.80 1416714_at Irf8 BG069095 1.64 1.32 1418104_at Nrip3 NM_020610 1.58 0.58 1428142_at Etv5 AK003461 1.64 1.63 1416093_a_at Mrpl20 NM_025570 1.58 1.17 1436094_at Vgf BF458396 1.64 1.57 1426187_a_at Hax1 AF465243 1.58 1.16 1452168_x_at Gspt1 AB003502 1.64 1.29 1439605_at BB464198 1.58 0.69 1456964_at Rbm12 BB104271 1.63 0.76 1425660_at Btbd3 BC018174 1.57 2.19 1424686_at 2700008B19Rik BF150550 1.63 0.96 1423294_at Mest AW555393 1.57 1.22 1449342_at Ptplb NM_023587 1.63 0.94 1416085_s_at Zfand5 AA124553 1.57 1.11 1423314_s_at Pde7a BG070255 1.63 1.47 1421280_at Gabra1 Z36357 1.57 0.66 1430520_at Cpne8 AW548480 1.63 0.47 1424405_at Mbip BC002277 1.57 0.90 1439293_at BC031353 BB369212 1.63 0.87 1453020_at 1810048J11Rik AK007829 1.57 1.05 1426934_at Nhsl1 BC013565 1.63 1.44 1428920_at Hmg20a AI987819 1.57 1.13 1429249_at 4833424O15Rik BB346520 1.63 1.19 1450247_a_at Scamp5 NM_020270 1.57 0.89 1436645_a_at Cnot4 BB066603 1.62 1.04 1456796_at Snai3 BB238756 1.57 1.13 1459858_x_at Crtac1 AV354465 1.62 1.11 1438682_at Pik3r1 BB426164 1.56 1.00 1437114_at A930001N09Rik AV230892 1.62 1.10 1439900_at Thtpa BB308405 1.56 1.55 1441197_at 9530059O14Rik AW494454 1.62 1.47 1423648_at Pdia6 BC006865 1.55 1.07 1442212_at Cpd BB519218 1.62 1.36 1434246_at L3mbtl3 BB022070 1.55 1.08 1449799_s_at Pkp2 AA516617 1.62 1.53 1427358_a_at Dapk1 BC026671 1.55 0.80 1420947_at Atrx BB825830 1.62 0.51 1435357_at D4Wsu53e BE652553 1.55 1.13 1434228_at Ppm2c AV255921 1.62 1.09 1460440_at Lphn3 BE945410 1.55 1.04 1427470_s_at Napb AU067119 1.62 0.88 1424342_at Fyttd1 BC010204 1.55 1.08 1430511_at 1500037O19Rik AK005367 1.62 1.15 1425608_at Dusp3 BC016269 1.55 1.18 1443017_at Cpeb2 BB235708 1.61 1.11 1426824_at Psme4 BM195254 1.55 1.10 1417404_at Elovl6 NM_130450 1.61 1.30 1424532_at Ylpm1 AB033168 1.55 1.14 1419211_s_at 4933424B01Rik NM_138757 1.61 1.03 1426490_at Bfar AK013874 1.55 0.69 1423162_s_at Spred1 BQ044290 1.61 1.39 1424350_s_at Lpgat1 BE987427 1.55 1.20 1448669_at Dkk3 AK004853 1.61 1.42 1457277_at BC038925 AI314927 1.55 1.14 1428999_at Ph13 AK010013 1.61 0.79 1435641_at 9530018I07Rik BB109391 1.55 1.27 1440891_at Gria4 AV336506 1.61 1.49 1422553_at Pten AA214868 1.55 1.10 1434883_at Mtdh AV083741 1.61 0.82 1455009_at Cpd AW550842 1.55 1.19 1421815_at Epdr1 AF353717 1.60 1.63 1433581_at 1190002N15Rik AV309085 1.54 1.21 1417416_at Kcna1 NM_010595 1.60 1.12 1419256_at Spnb2 BM213516 1.54 1.22 1433887_at Dnajc3 AV024539 1.60 1.24 1428793_at Slc36a1 AK017918 1.54 1.20 1420580_at 4930429B21Rik NM_026249 1.60 0.91 1418772_at BC016423 NM_134063 1.54 0.75 1435250_at Ints8 BB206299 1.60 1.39 1423247_at Txndc4 BI100077 1.54 0.95 1421768_a_at Homer1 NM_011982 1.60 0.96 1434283_at Arid5b BB079486 1.54 1.60 1417977_at Eif4e3 BCO27014 1.60 0.83 1424089_a_at Tcf4 U16321 1.53 1.13 1419988_at Map3k7 C87327 1.60 1.12 1426023_a_at Rabep1 AF248489 1.53 1.04 1421346_a_at Slc6a6 NM_009320 1.60 1.04 1441603_at Sstr3 BQ174132 1.53 1.86 1428344_at Ppapdc2 BB229589 1.60 1.25 1443969_at Irs2 BE199054 1.53 1.20 1439661_at Slc16a14 BB426676 1.59 0.86 1450011_at Hsd17b12 AK012103 1.53 1.03 1419110_at Riok1 BM228482 1.59 0.78 1454816_at Rp2h BB431808 1.53 0.48 1415957_a_at Nnp1 AV297071 1.59 0.83 1424244_at Rwdd4a AK005797 1.53 1.30 1416479_a_at Tmem14c NM_025387 1.59 0.68 1425014_at Nr2c2 AU066920 1.53 1.11 1430274_a_at Stard3nl AK018331 1.59 1.01 1425713_a_at Rnf146 BC019182 1.53 1.30 1450213_at Pde7b NM_013875 1.59 1.52 1450184_s_at Tef NM_017376 1.53 1.00 1419650_at Zfr NM_011767 1.59 0.88 1439764_s_at Igf2bp2 BB098431 1.53 1.20 1420838_at Ntrk2 AK018789 1.59 1.58 1438473_at Arl15 BB445175 1.53 0.97 1428461_at Ppp2r5e BB535888 1.59 1.12 1440886_at Cdc37l1 BE949427 1.53 1.65 1436763_a_at Klf9 AI267126 1.59 0.84 1451622_at Lmbrd1 BM206793 1.53 1.21 1417818_at Wwtr1 BC014727 1.58 0.97 1429265_a_at Rnf130 AK011088 1.53 0.93 1420514_at Tmem47 NM_138751 1.52 1.12 1450744_at Ell2 NM_138953 1.48 0.92 1419444_at Sap18 NM_009119 1.52 1.11 1423503_at Jam3 AK017692 1.47 1.32 1421867_at Nr3c1 NM_008173 1.52 0.94 1448376_at Wrnip1 NM_030215 1.47 1.11 1455020_at Snx25 AV351081 1.52 1.14 1419580_at Dlg4 AI646416 1.47 0.58 1427311_at Bptf BB380312 1.52 1.07 1424749_at Wdfy1 BC025226 1.47 1.32 1451018_at Leprotl1 BF658789 1.52 1.15 1427427_at Ryr3 AV238793 1.47 1.13 1420833_at Vamp2 BG871810 1.52 1.26 1431020_a_at Fgfr1op2 AK008983 1.47 0.95 1450174_at Ptprt NM_021464 1.52 1.25 1416123_at Ccnd2 NM_009829 1.47 1.55 1425563_s_at Pcdh10 AF334801 1.51 1.48 1422621_at Ranbp2 BM507707 1.47 1.53 1428523_at Lphn3 BE945410 1.51 0.98 1434877_at Nptx1 AI152800 1.47 1.19 1450907_at Spcs2 BI078449 1.51 0.86 1420849_at Crnkl1 AV143435 1.47 1.06 1437318_at Pak3 BB468082 1.51 0.99 1420661_a_at 4933439F18Rik NM_025757 1.47 1.64 1448896_at Pigf NM_008838 1.51 1.08 1426491_at Herc2 AW701798 1.47 1.43 1422607_at Etv1 NM_007960 1.51 1.36 1428998_at Phf3 AK010013 1.47 0.93 1452757_s_at Hba-a1 AK011116 1.51 1.16 1444015_at Papola AV307016 1.47 0.76 1423060_at Pa2g4 BM232515 1.51 0.79 1434353_at Sfmbt2 BM200222 1.46 0.55 1438407_at Dsel AV336691 1.51 0.95 1417538_at Slc35a1 NM_011895 1.46 0.84 1426301_at Alcam U95030 1.51 0.58 1445843_at BE199465 1.46 0.82 1450668_s_at Hspe1 NM_008303 1.50 1.07 1419914_s_at D10Ertd438e AW538011 1.46 1.25 1418318_at Rnf128 AK004847 1.50 0.88 1436788_at Acp2 AI131584 1.46 1.04 1429857_at Pou3f3 BB807002 1.50 1.22 1419392_at Pclo NM_011995 1.46 1.14 1453380_a_at Xrcc6bp1 AK010471 1.50 1.57 1421990_at Syt1 NM_009306 1.46 1.30 1426258_at Sorl1 BI648081 1.50 1.13 1416133_at C920006C10Rik BB188557 1.46 1.18 1432287_a_at Sntg1 AK016927 1.50 0.92 1416726_s_at Ube2s NM_133777 1.46 0.97 1419035_s_at Csnk2a1 BB283759 1.50 1.08 1417924_at Pak3 BQ174935 1.46 1.12 1425929_a_at Rnf14 AF249668 1.50 1.06 1420165_s_at Dnajc17 N28111 1.46 0.90 1447771_at AW050081 1.50 0.49 1418084_at Nip1 AK011144 1.46 1.60 1450120_at Scn1a AV336781 1.50 1.12 1423753_at Bambi AF153440 1.46 0.94 1460061_at Adra1a AV342748 1.50 1.06 1430134_a_at Yars2 AK008774 1.46 1.19 1421353_at Pde7b NM_013875 1.50 1.29 1425060_s_at Clip1 BG969810 1.45 1.15 1431094_at 1110006E14Rik AK003491 1.50 0.81 1425855_a_at Crk AF239673 1.45 1.05 1434937_at Phr1 AW546647 1.50 0.96 1428620_at Ensa BF584204 1.45 1.24 1429330_at Gabra4 AK013727 1.50 0.95 1417878_at E2f1 NM_007891 1.45 1.33 1450051_at Atrx BB825830 1.50 0.86 1429336_at Tmem87b BB161454 1.45 0.97 1452714_at Tanc1 AK004560 1.49 1.07 1423211_at Nola3 AK004120 1.45 1.09 1427988_s_at Safb2 BM239918 1.49 1.30 1460336_at Ppargc1a BB745167 1.45 0.95 1452335_at 2810423E13Rik AV024565 1.49 0.72 1416266_at Pdyn AF026537 1.45 1.76 1429045_at Smurf2 BI697997 1.49 1.09 1437366_at AI608492 BB332542 1.45 1.02 1436451_a_at Tmed2 AW824211 1.49 1.06 1438452_at Nebl BM121794 1.45 0.86 1425132_at Neto1 AF448840 1.49 1.15 1452894_at Elavl4 AK013588 1.45 0.54 1435226_at Ibrdc3 BG064140 1.49 1.23 1426885_a_at Cdk2ap1 AK004852 1.45 1.06 1430780_a_at Pmm1 BI739353 1.49 0.80 1424886_at Ptprd BC025145 1.45 0.94 1434736_at Hlf BB744589 1.49 1.21 1434956_at AI481227 AV145226 1.45 0.85 1437481_at 1110039F03Rik AV032349 1.49 0.93 1452813_a_at 5033428A16Rik AK017198 1.45 1.33 1426300_at Alcam U95030 1.49 0.84 1455309_at Tmem16f BG074632 1.45 1.15 1434386_at Atp2c1 BM225269 1.49 1.11 1419398_a_at Reep5 NM_007874 1.45 0.96 1434206_s_at Ppp2r5c BM247370 1.48 1.34 1429475_at 2810457I06Rik AK013361 1.45 2.15 1437560_at Ntrk2 BQ174783 1.48 1.26 1428237_at 2700059D21Rik BI689227 1.44 1.15 1450569_a_at Rbm14 NM_019869 1.48 1.22 1452271_at Xpr1 AV337591 1.44 1.10 1448346_at Cfl1 NM_007687 1.48 0.97 1457293_at BF714580 1.44 1.01 1455365_at Cdh8 BB426483 1.48 1.04 1426613_a_at Snrpb2 AV066554 1.44 1.12 1426455_at Sdccag10 AK014025 1.48 0.84 1426310_at Zdhhc5 AV241525 1.44 1.68 1426397_at Tgfbr2 BG793483 1.48 1.37 1455215_at C530028O21Rik AU067746 1.44 1.04 1433772_at Stch BE650268 1.48 1.23 1427308_at Dab1 BB64109 1.44 1.04 1451147_x_at Csdc2 BC016109 1.48 1.38 1434306_at Rab3ip BF19015 1.44 1.33 1436473_at Zfp248 AV300441 1.44 0.48 1416711_at Tbr1 NM_009322 1.41 1.19 1420922_at Usp9x AW107303 1.44 0.93 1453264_at Marveld3 AK007346 1.41 0.58 1432435_s_at C030004A17Rik AK012261 1.44 1.35 1454771_at AW060701 1.41 1.21 1438975_x_at Zdhhc14 AV361868 1.44 1.44 1416510_at Mrpl4 NM_023167 1.41 1.13 1416328_a_at Atp6v0e NM_025272 1.44 1.15 1448344_at Rps12 NM_011295 1.41 0.93 1419397_at Pola1 NM_008892 1.44 1.03 1451572_a_at 5230400G24Rik BC016597 1.41 1.00 1416288_at Dnaja1 BF141076 1.44 1.06 1418367_x_at Hist2h2aa1 BC010564 1.41 1.08 1429564_at Pcgf5 AK002387 1.44 1.35 1425780_a_at Tmem167 BC024352 1.41 0.98 1439573_at Rtn4rl2 BE992565 1.44 2.15 1455632_at Gnb5 BE446953 1.41 1.75 1438258_at Vldlr BE647363 1.43 1.21 1416567_s_at Atp5e NM_025983 1.41 1.20 1460441_at Zxdb BF660409 1.43 0.98 1418469_at Nrip1 NM_008735 1.41 1.40 1417106_at Tpd52l2 NM_025482 1.43 1.22 1418386_at N6amt2 NM_026526 1.41 1.15 1425480_at Cnot6l BC018506 1.43 1.00 1453077_a_at Snapc3 AK016168 1.41 0.97 1428184_at 3110035E14Rik BB348639 1.43 1.21 1433868_at Btbd3 AV028445 1.41 1.09 1436985_at Zfp644 BM115349 1.43 0.69 1434604_at Eif5b BM236870 1.41 1.16 1439847_s_at Klf12 BM249597 1.43 1.12 1437118_at Usp7 C77542 1.41 1.04 1450773_at Kcnd2 BB051684 1.43 0.86 1453406_a_at Rab28 AK012286 1.41 1.12 1451270_at Dusp18 BC020036 1.43 1.00 1435553_at Pdzd2 AV376136 1.40 1.47 1450949_at Katna1 AK012319 1.43 0.80 1416214_at Mcm4 BC013094 1.40 0.82 1419169_at Mapk6 BC024684 1.43 1.11 1449507_a_at Cd47 NM_010581 1.40 1.01 1437021_at Arl13b AV225959 1.43 0.91 1457587_at Ga17 BB196645 1.40 0.97 1448882_at Tmem93 NM_025318 1.43 1.08 1416566_at Strap NM_011499 1.40 1.02 1429488_at Zdhhc21 AK017682 1.43 1.07 1417612_at Ier5 BF147705 1.40 1.18 1436169_at C730029A08Rik BB428892 1.43 1.21 1460174_at Dexi NM_021428 1.40 1.23 1455944_at Zfp516 BB467812 1.43 0.78 1423172_at Napb BB147418 1.40 0.89 1436796_at 1110061A14Rik BB237072 1.43 0.83 1449324_at Ero1l BM234652 1.40 1.21 1419280_at Pip5k2a AK012196 1.43 0.70 1423477_at Zic1 BB361162 1.40 0.79 1420537_at Kctd4 NM_026214 1.43 0.86 1452905_at Gtl2 AV015833 1.40 0.68 1418927_a_at Habp4 NM_019986 1.43 1.05 1442257_at Cdh6 BI134319 1.40 1.56 1434791_at Atp6v0a2 BM229554 1.43 1.45 1417933_at Igfbp6 NM_008344 1.39 1.65 1454987_a_at H2-Ke6 A1323545 1.43 1.10 1453286_at Plxna2 BB085537 1.39 1.64 1441371_at Plxna4 BQ174019 1.43 0.93 1429779_at Eif2c4 A1481660 1.39 1.47 1442051_at Hist2h3c1 BE691662 1.43 1.19 1419127_at Npy NM_023456 1.38 1.65 1427683_at Egr2 X06746 1.43 1.27 1435891_x_at 2610021A01Rik BQ175465 1.38 0.53 1433532_a_at Mbp AI323506 1.43 1.20 1441961_at Mtap9 BB698742 1.37 0.68 1436868_at Rtn4rl1 BM508396 1.43 1.14 1452370_s_at B230208H17Rik BB449608 1.37 0.59 1442027_at Nbeal1 BB223743 1.43 1.56 1454701_at 4930503L19Rik AI450962 1.37 0.59 1423316_at Tmem39a AK017817 1.42 0.91 1424373_at Armcx3 AK004598 1.37 1.45 1426743_at Appl2 BC002232 1.42 1.21 1418003_at 1190002H23Rik NM_025427 1.36 1.78 1431024_a_at Arid4b AK020165 1.42 0.54 1433733_a_at Cry1 BG069864 1.36 1.57 1453787_at Txndc13 AK015667 1.42 1.10 1434202_a_at BC055107 BF682848 1.35 1.42 1431711_a_at 9030409G11Rik AK018497 1.42 1.88 1416316_at Slc27a2 BC013442 1.35 0.58 1422968_at Ihpk1 NM_013785 1.42 0.91 1423278_at Ptprk AI893646 1.35 1.73 1421193_a_at Pbx3 NM_016768 1.42 1.01 1456685_at Nsg2 BB227199 1.34 1.75 1423198_a_at Smek2 AV305633 1.42 0.88 1445669_at Spry4 BB080456 1.34 2.12 1422517_a_at Znrd1 NM_023162 1.42 0.88 1440437_at Herc1 BM247513 1.34 0.51 1423501_at Max AA617392 1.42 0.95 1458358_at Pank2 BB402666 1.34 0.41 1451486_at 1200006F02Rik BC006902 1.42 0.86 1449885_at Tmem47 NM_138751 1.34 1.69 1419954_s_at Zfand3 AW539211 1.42 1.02 1433864_at Lrp12 AV254798 1.34 1.51 1435244_at Vav2 BF535947 1.42 0.92 1456388_at Atp11a AV378604 1.34 1.72 1437614_x_at Zdhhc14 AV223474 1.42 1.42 1416630_at Id3 NM_008321 1.33 1.61 1437858_at Dpy19l3 AV367203 1.42 1.05 1427134_at Sfrs12 AV012790 1.33 1.41 1455076_a_at 4933424B01Rik AV255808 1.42 0.80 1429150_at Jarid1a BM224226 1.33 1.52 1455113_at Armc8 BM232782 1.42 1.01 1452868_at Usp24 AK012842 1.33 1.66 1435451_at Hel308 BG068026 1.42 1.54 1447275_at Bbs12 AI449447 1.33 1.65 1435616_at Cyp20a1 BE686557 1.33 1.51 1424169_at Tax1bp3 BC008166 1.21 0.50 1457670_s_at Lmna AV238225 1.33 0.61 1426373_at Ski AK019148 1.21 1.42 1446090_at BB521597 1.32 1.50 1415840_at Elovl5 NM_134255 1.21 1.43 1416432_at Pfkfb3 NM_133232 1.32 1.56 1426047_a_at Ptprr AF129509 1.21 0.71 1453172_at Stch BE533039 1.32 1.41 1453166_at Ccdc109a AK008216 1.20 0.64 1435156_at BC046331 BF730275 1.32 1.55 1421148_a_at Tial1 NM_009383 1.20 0.68 1419182_at Svep1 NM_022814 1.31 1.90 1427064_a_at Scrib BC006859 1.20 1.41 1456946_at Sh3md4 BE949337 1.31 1.55 1426434_at Tmem43 AI747289 1.20 0.47 1423104_at Irs1 BB345784 1.30 1.45 1440504_at AI844750 BB397002 1.20 0.50 1419453_at Uchl5 AV313813 1.30 0.50 1451525_at Arhgap12 BG064038 1.19 0.69 1423337_at Orc4l BB775020 1.29 0.71 1417675_a_at Mdn1 NM_133874 1.19 1.53 1428083_at 2310043N10Rik AK018202 1.29 1.44 1420845_at Mrps2 AV031454 1.19 1.43 1449913_at Zfp2 NM_009550 1.29 0.67 1422675_at Smarce1 AK018493 1.19 0.69 1455876_at Slc4a7 BM237826 1.29 0.55 1420485_at Nol7 AW060738 1.19 0.66 1436103_at Rab3ip AV235634 1.29 1.44 1460300_a_at Ltk NM_008523 1.19 1.54 1436758_at Hdac4 AI661423 1.29 1.43 1418595_at S3-12 NM_020568 1.19 2.42 1422320_x_at Phxr5 NM_008836 1.28 3.21 1459917_at Ggnbp2 BG078867 1.19 0.64 1436137_at Slc6a17 AV340147 1.28 1.65 1428340_s_at Atp13a2 BM944122 1.19 1.45 1426852_x_at Nov X96585 1.28 1.77 1454962_at Spire1 AU067702 1.18 1.46 1456753_at AW209075 1.28 1.41 1423475_at Cnnm2 BB278418 1.18 1.46 1457693_a_at 6430537H07Rik AV343405 1.28 2.14 1438724_at Osbpl3 AI875442 1.18 1.41 1418966_a_at Dcbld1 AK014521 1.28 1.64 1438838_at B230206F22Rik BG141977 1.18 0.49 1455164_at Cdgap AV308092 1.28 1.42 1440688_at Arhgap26 BM244666 1.18 1.60 1455518_at BB324402 1.27 1.45 1435304_at Sod1 BM240246 1.18 0.71 1454014_a_at Mkks AK004987 1.26 1.57 1424160_at Alg5 BC027160 1.18 0.59 1418587_at Traf3 U21050 1.26 1.53 1426886_at Cln5 AV315220 1.18 1.48 1448569_at Cd8b1 BF682469 1.26 1.47 1439615_at Gan BB187898 1.18 1.49 1422533_at Cyp51 NM_020010 1.26 1.48 1454693_at Hdac4 BQ176116 1.18 1.48 1435229_at Gramd1b BI729743 1.26 1.42 1455260_at Lcorl BE650208 1.17 0.63 1439019_at Fras1 BB541392 1.26 1.73 1422910_s_at Smc6 AU022584 1.17 0.57 1420807_a_at Egfl9 NM_134120 1.25 1.48 1449351_s_at Pdgfc NM_019971 1.17 1.48 1457694_at 6430537H07Rik AV343405 1.25 1.71 1423269_a_at Nedd4l BB663717 1.17 1.40 1417219_s_at Tmsb10 NM_025284 1.25 1.52 1435402_at Gramd1b BB066186 1.17 1.51 1440308_at Cmpk AV347071 1.25 0.67 1444359_at Zfp777 BB354954 1.17 0.51 1460426_at Pde4dip AI639670 1.25 1.47 1451227_a_at Slc10a3 BC027440 1.17 1.47 1422165_at Pou3f4 X66603 1.24 1.44 1427004_at Fbxo2 BB311718 1.17 1.61 1436588_at Rttn BB700837 1.24 1.53 1430986_at Farslb AK012154 1.17 2.67 1427035_at Slc39a14 BB399837 1.24 1.46 1453556_x_at Cd99 AK002762 1.17 1.43 1432431_s_at 2900006F19Rik AK004072 1.24 1.61 1457948_at BF456094 1.16 0.54 1451753_at Plxna2 D86949 1.24 1.69 1427287_s_at Itpr2 BB216794 1.16 1.62 1452123_s_at Frmd4b BG067753 1.24 1.48 1419672_at Spock1 BB308491 1.16 0.59 1420579_s_at Cftr NM_021050 1.23 1.89 1437252_at Gats BB768012 1.16 1.43 1437291_at 2700081O15Rik AV131166 1.23 0.55 1455086_at Uchl5 AV312620 1.16 0.47 1424590_at Ddx19b BC002300 1.23 1.55 1427261_at Wwc1 BQ176786 1.16 1.44 1427902_at Srrm2 BQ176063 1.23 0.63 1456256_at Eif5 BM242785 1.16 1.66 1431541_at Stt3b AK016943 1.23 1.89 1419380_at Zfp423 NM_033327 1.16 1.56 1420530_at Neud4 AW553317 1.23 0.57 1435642_at Sft2d1 BB081437 1.16 1.51 1438007_at AI851790 BB432758 1.22 0.58 1446357_at BC020402 BB029175 1.16 0.71 1427343_at Rasd2 BC026377 1.22 1.82 1455150_at BB038506 1.16 0.63 1457440_at Sstr4 BB451927 1.22 1.40 1455601_at BB507994 1.16 0.69 1441396_at B3galt1 AV328619 1.22 1.76 1421819_a_at Set BF134272 1.15 0.68 1450684_at Etv1 NM_007960 1.22 1.53 1427785_x_at Solh BC022685 1.15 1.41 1437049_at BE627361 1.21 0.51 1428166_at Cdan1 AK005239 1.15 1.51 1448918_at Slco3a1 NM_023908 1.21 1.69 1416246_a_at Coro1a BC002136 1.15 1.65 1423137_at Rala BG073338 1.21 0.64 1427542_at 5330439J01Rik BC027318 1.15 1.74 1454824_s_at Pdgfrl BB699957 1.15 1.44 1455622_at Podxl2 BB461988 1.10 1.90 1417379_at Iqgap1 NM_016721 1.15 1.46 1416444_at Elovl2 NM_019423 1.10 1.76 1425731_at Ankrd24 BC017633 1.15 0.55 1435031_at 2010310D06Rik AV069499 1.10 1.53 1415772_at Ndl BF118393 1.15 0.58 1453434_at 1110019D14Rik AK003812 1.10 1.86 1423213_at Plxnc1 BB476707 1.15 1.46 1416683_at Plxnb2 NM_138749 1.10 1.52 1438011_at Pcyt1a BB280291 1.15 1.50 1437983_at Sall1 BB739342 1.10 1.76 1434505_a_at 6430548M08Rik BF465109 1.15 2.11 1455014_at AV009015 BM213104 1.10 0.66 1451474_a_at Parp8 BC022679 1.14 2.13 1449476_at Rage NM_011973 1.10 0.71 1454878_at 2310047C04Rik BM936291 1.14 0.57 1453500_at Cyp2u1 AK018458 1.10 1.58 1439380_x_at Gtl2 BB093563 1.14 0.66 1434826_at Rfesd AW124912 1.09 0.63 1419757_at Pitpnm2 NM_011256 1.14 1.77 1417216_at Pim2 NM_138606 1.09 0.71 1448655_at Lrp1 NM_008512 1.14 1.47 1425067_at Celsr3 AF427498 1.09 1.73 1434215_at B230308N11Rik AW554529 1.14 1.54 1436030_at Cachd1 BB730977 1.09 1.82 1441636_at BB133117 1.14 1.43 1426979_at Mlxip AF265663 1.09 1.56 1452199_at 2700094F01Rik BB667255 1.14 0.68 1454136_a_at 4921524J17Rik AK013123 1.09 0.62 1416803_at Fkbp7 NM_010222 1.13 0.56 1456697_x_at Dintf1 BB248138 1.09 2.03 1425732_a_at Mxi1 D31824 1.13 1.50 1451156_s_at Vldlr BB628702 1.09 0.70 1436515_at E030004N02Rik BB529913 1.13 1.98 1422742_at Hivep1 NM_007772 1.09 1.74 1426577_a_at 1810054G18Rik BM570079 1.13 1.45 1455448_at Nt5dc3 BG804066 1.08 1.41 1437813_at Aim1l BE948330 1.13 0.66 1423560_at Nell2 AI838010 1.08 1.93 1432156_a_at Rnf32 AK012288 1.13 1.56 1454036_a_at Usp15 AK014891 1.08 1.49 1417312_at Dkk3 AK004853 1.13 1.71 1453246_at Rab39b AK020665 1.08 0.65 1427978_at 4732418C07Rik BB318254 1.13 1.50 1454606_at 4933426M11Rik BF144564 1.08 1.42 1436630_at Aqp11 AI550443 1.13 1.49 1421191_s_at Gopc NM_053187 1.08 1.50 1428301_at LOC554327 BM195235 1.12 1.50 1451332_at Zfp521 BC021376 1.08 1.47 1456316_a_at Acbd3 BI965035 1.12 0.30 1442739_at BC031441 BF225404 1.07 0.55 1440930_a_at Scrt2 BG807042 1.12 0.68 1449390_at Gpatc4 NM_025663 1.07 1.63 1429984_at 5730455O13Rik AK017666 1.12 0.49 1454756_at Lrch3 BB763476 1.07 2.17 1424523_at Elmo1 BCO24727 1.12 1.59 1417303_at Mvd NM_138656 1.07 0.69 1454720_at Hs3st5 AV328620 1.12 1.64 1417551_at Cln3 NM_009907 1.07 1.52 1418025_at Bhlhb2 NM_011498 1.12 1.66 1437020_at Ep400 BM236717 1.07 0.43 1439561_at 2010012O05Rik BB322051 1.12 0.51 1416589_at Sparc NM_009242 1.07 1.71 1418045_at Inpp1 NM_008384 1.12 1.44 1440972_at Nsd1 AI413236 1.07 0.64 1427209_at Baz2a AW910654 1.12 0.66 1442220_at BB313387 1.07 1.53 1443493_at Dhx37 BB766805 1.12 0.46 1418313_at Zfp276 BB667131 1.07 1.50 1417567_at Ctnnbip1 BF457754 1.11 1.45 1415874_at Spiy1 NM_011896 1.06 1.52 1420934_a_at Srrm1 NM_016799 1.11 0.58 1415758_at Fryl BM118442 1.06 1.48 1426594_at Frmd4b BG067753 1.11 1.47 1433682_at Arhgef17 BE287052 1.06 1.42 1450661_x_at Nfic NM_008688 1.11 1.64 1442917_at BM195384 1.06 1.63 1452306_at Zfyve26 BC006654 1.11 1.45 1438363_at EG434128 BB332375 1.06 1.57 1415692_s_at Canx BI653492 1.11 0.70 1440215_at ″OTTMUS 1416049_at Gldc NM_138595 1.11 1.51 G0000000446 BG068916 1.06 0.62 1435952_at Tsc22d1 AV118079 1.11 1.93 1″ 1425492_at Bmpr1a BM939768 1.11 1.43 1435737_a_at Nde1 BQ032232 1.05 1.45 1429489_at Rexo1 AK012127 1.11 1.74 1437891_at Frs2 AV331887 1.05 1.55 1420925_at Tub NM_021885 1.11 1.45 1444072_at AW123332 1.05 0.59 1448396_at Tmem131 NM_018872 1.11 1.48 1429440_at 1810041L15Rik BI734299 1.05 1.74 1455153_at Zfp236 BQ177220 1.11 1.53 1435527_at Nfic BB533448 1.05 1.42 1419362_at Mrpl35 BF787384 1.11 0.53 1440291_at BE954335 1.05 0.62 1450135_at Fzd3 AU043193 1.10 0.62 1416187_s_at Pnrc2 NM_026383 1.05 0.68 1429224_at Pnmal AK017476 1.10 0.70 1442754_at C030013G03Rik BE692283 1.05 1.99 1451362_at Rab7l1 BC016133 1.10 1.49 1443770_x_at Auts2 BB026407 1.05 1.99 1455520_at LOC629605 AV327200 1.10 2.74 1428466_at Chd3 AK011183 1.05 1.50 1436302_at Slc10a7 BB770006 1.10 1.55 1448733_at Bmi1 M64279 1.05 0.63 1435488_at BB427289 1.10 1.68 1460001_at Pdpep1 AI642171 1.05 0.66 1430814_at 1300013D18Rik AK004984 1.05 1.43 1452029_a_at Purg AF479673 0.99 1.54 1447550_at Zfp644 BB545511 1.05 0.62 1429185_at 4631416L12Rik BB774721 0.99 1.81 1449349_at Nudt1 NM_008637 1.04 0.61 1420375_at Kif3a NM_008443 0.99 0.59 1421252_a_at Mef2a NM_013597 1.04 0.63 1435810_at 5730455O13Rik AV297833 0.99 0.71 1434654_at Cog3 BE570422 1.04 1.50 1444736_at Cdh7 BB129525 0.99 1.48 1439783_at C330018D20Rik BB809870 1.04 0.68 1421392_a_at Birc3 NM_007464 0.98 1.72 1422635_at Ache NM_009599 1.04 2.16 1424138_at Rhbdf1 BC027346 0.98 1.42 1425423_at Glis1 AF220434 1.04 0.61 1452347_at Mef2a AV255689 0.98 0.68 1435719_at AI448984 BM935216 1.04 0.62 1417870_x_at Ctsz NM_022325 0.98 1.50 1424042_at Tmem5 BC020100 1.04 0.67 1435335_a_at Gnptab BG144467 0.98 1.42 1454198_a_at Efcab1 AK015866 1.04 0.58 1439506_at Gm98 BB193557 0.98 1.49 1439817_at 2900064A13Rik AI451465 1.04 0.70 1447230_at AI553587 BE954466 0.98 0.63 1428403_at 2410025L10Rik AV330483 1.04 1.95 1455293_at Leo1 BG065311 0.98 0.63 1455333_at Tns3 AI315031 1.03 1.43 1416806_at Fdxr NM_007997 0.97 0.70 1455700_at Mterfd3 BB359043 1.03 0.65 1430776_s_at Ankrd24 AK017778 0.97 0.66 1427172_at Ofd1 AJ278702 1.03 0.49 1436200_at Lonrf3 BE956940 0.97 1.52 1428431_at 2310047A01Rik BB813478 1.03 1.57 1428794_at Specc1 BQ175052 0.97 1.55 1460394_a_at Inppl1 BB769433 1.03 1.47 1434600_at Tjp2 BB758095 0.97 1.49 1455275_at E530001K10Rik AW046441 1.03 0.45 1428732_at 1700008J07Rik AK005774 0.97 0.61 1456520_at Zfp652 BF147545 1.03 1.57 1426865_a_at Ncam1 BB698413 0.97 0.56 1435295_at Dopey1 BG075614 1.03 1.45 1422612_at Hk2 NM_013820 0.97 1.52 1422324_a_at Pthlh NM_008970 1.03 1.63 1436032_at BB201888 0.97 1.96 1435783_at B230112C05Rik BB476773 1.03 0.69 1426848_at Sec24b BE947982 0.97 1.49 1458307_at B230334C09Rik BE992834 1.03 0.51 1431072_a_at Ccdc50 BB865814 0.97 1.59 1426405_at Rnf11 BI150320 1.02 0.69 1455906_at 6030446N20Rik AV222909 0.97 0.59 1455758_at Prkcc BM215011 1.02 1.62 1439558_at Zfp75 BM729399 0.97 0.68 1425118_at Spire2 BC026502 1.02 1.68 1443528_at Ttbk1 BB398560 0.97 1.55 1452267_at Flywch1 BB477613 1.02 0.49 1425968_s_at Speg AF215896 0.96 1.52 1429684_at 5830472M02Rik BG094398 1.02 2.23 1452604_at Stard13 BB667840 0.96 1.47 1421830_at Ak3l1 NM_009647 1.02 1.48 1426316_at 6330416G13Rik AV326978 0.96 0.71 1450937_at Lin7c BQ176612 1.02 0.65 1427269_at Sfrs11 AW261583 0.96 0.61 1442415_at 5830454E08Rik BQ180241 1.02 0.64 1434952_at Cox4nb BI734783 0.96 1.45 1417577_at Trpc3 NM_019510 1.02 0.56 1444208_at Dnahc1 BE950899 0.96 0.57 1421586_a_at Cd46 NM_010778 1.02 0.62 1436707_x_at Ncaph BB725358 0.96 0.70 1434691_at Sfrs2ip BB154529 1.02 0.71 1428223_at Mfsd2 AK006096 0.96 1.82 1448554_s_at Myh7 NM_080728 1.02 0.69 1438476_a_at Chd4 BB201828 0.96 1.49 1457544_at Ube2w BB442807 1.01 0.59 1417384_at Entpd5 NM_007647 0.96 1.97 1429783_at Pdlim5 AK009464 1.01 0.48 1416953_at Ctgf NM_010217 0.96 1.62 1423738_at Oxa1l BC027191 1.01 1.40 1438478_a_at Ppp3ca AI313926 0.96 0.48 1425405_a_at Adar AF291876 1.01 0.71 1428198_at Adal AK016299 0.95 0.67 1449248_at Clcn2 NM_009900 1.01 0.70 1420402_at Atp2b2 NM_009723 0.95 1.97 1429836_at Ugcgl2 BM218963 1.01 1.93 1452055_at Ctdsp1 BB770944 0.95 1.51 1428352_at Arrdc2 AW542672 1.01 0.49 1439516_at 2610201A13Rik AW488471 0.95 0.66 1451625_a_at C8g BC019967 1.00 1.41 1433890_a_at Bat3 AW545877 0.95 1.46 1444001_at Strbp AW488249 1.00 0.52 1417937_at Dact1 NM_021532 0.95 1.61 1452661_at Tfrc AK011596 1.00 0.71 1418924_at Rassf7 NM_025886 0.95 0.68 1458492_x_at Hnt BB081359 1.00 0.67 1452831_s_at Ppat AV305746 0.95 0.67 1440884_s_at A530047J11Rik BB218047 1.00 0.55 1419905_s_at Hpgd AV026552 0.94 0.45 1419047_at Pcnx BG073499 1.00 1.43 1424125_at Kcnk13 BC023443 0.94 1.49 1423097_s_at Capn7 BQ257745 1.00 0.71 1429499_at Fbxo5 AK011820 0.94 0.70 1455497_at Leng9 BB799608 1.00 0.67 1442434_at D8Ertd82e BM195829 0.94 1.40 1436165_at Luc7l2 BI076494 1.00 0.63 1435324_x_at Hmgb1 AI648759 0.94 1.50 1458337_at Tcf12 BB476667 0.99 0.60 1448546_at Rassf3 BB703307 0.94 0.63 1456858_at Gpr149 BB075339 0.99 0.60 143989_at 2900054J07Rik BB326106 0.94 0.51 1438469_at Bcorl1 BG071122 0.99 1.42 1435617_at 1700106N22Rik BQ175501 0.94 0.40 1452338_s_at Itsn1 AA172344 0.94 2.52 1460520_at Mocs1 AK014178 0.89 0.67 1428870_at Nolc1 BM213850 0.94 0.68 1427544_a_at Papola U58135 0.89 0.68 1439463_x_at EG666836 AV127023 0.94 1.48 1448145_at Wwp2 AK004087 0.89 0.70 1439909_at Zfp521 BE133660 0.94 1.51 1428773_s_at Bcor AK018370 0.89 1.41 1425094_a_at Lhx6 AB031040 0.94 2.01 1424687_at 2700008B19Rik BF150550 0.89 1.79 1430435_at Aff3 AK014322 0.94 1.96 1419292_at Htra3 NM_030127 0.89 0.68 1439684_at 4930570G19Rik BB428007 0.94 0.63 1417194_at Sod2 NM_013671 0.89 0.59 1455463_at Phyhip BB427286 0.94 0.60 1429573_at Dmrtc1a AK014934 0.89 0.70 1422725_at Mak BG069426 0.94 1.48 1457811_at BM217632 0.89 1.52 1457536_at Gpc5 BB171986 0.94 2.03 1415680_at Anapc1 NM_008569 0.89 1.45 1421892_at St3gal2 BB750118 0.93 1.42 1443210_at BB354985 0.88 0.62 1422881_s_at Sypl BE333485 0.93 0.69 1434762_at Tmem142b BF457736 0.88 0.66 1431820_at 4632404H12Rik AK014549 0.93 0.66 1424571_at Ddx46 BF023426 0.88 0.50 1449857_at 1200011I18Rik NM_026177 0.93 0.66 1438210_at Gpr149 BB126999 0.88 0.55 1434610_at Plec1 BM210485 0.93 1.74 1420391_at Pard3 NM_033620 0.88 1.70 1426292_at 6330581L23Rik BG068796 0.93 1.42 1437511_x_at Clcc1 BB100861 0.88 0.69 1424400_a_at Aldh1l1 AK007822 0.93 0.71 1434950_a_at Armc8 BE995635 0.87 0.63 1425391_a_at Osbpl5 AB074008 0.93 1.46 1440997_at Tnrc6c AV236734 0.87 0.58 1420487_at Nol7 AW060738 0.93 0.63 1452618_at Cdk5rap2 BM230774 0.87 1.59 1427186_a_at Mef2a AV255689 0.93 0.66 1441534_at C86753 BG067565 0.87 1.81 1435555_at Cd247 AW552088 0.93 1.42 1456781_at AW121365 0.87 0.66 1427138_at 0610010D24Rik AW556861 0.92 1.56 1425489_at Slu7 BC025870 0.87 0.71 1429087_at 1110054O05Rik AK005245 0.92 0.67 1427911_at 2610307O08Rik AK012006 0.87 1.67 1418252_at Padi2 NM_008812 0.92 0.57 1448417_at Ninj1 NM_013610 0.87 0.69 1435110_at Unc5b BG065285 0.92 1.57 1435438_at Sox8 AV345303 0.87 0.67 1419313_at Ccnt1 NM_009833 0.92 1.41 1435456_at Ttc28 AW552254 0.86 1.46 1433979_at Rbms2 BQ176636 0.92 1.45 1448384_at Pofut2 BC018194 0.86 1.52 1439239_at Lin7b BB806633 0.92 1.44 1454969_at Lypd6 AV299725 0.86 1.49 1429517_at Zfyve20 BC017622 0.92 1.54 1440709_at C130081A10Rik BB375586 0.86 0.58 1436726_s_at Sptlc1 BB470898 0.92 0.69 1417489_at Npy2r NM_008731 0.86 1.55 1452127_a_at Ptpn13 BM236743 0.92 1.70 1440251_s_at Zfp64 BE947768 0.86 0.61 1421112_at Nkx2-2 NM_010919 0.92 1.60 1435279_at BC059842 AV338182 0.85 1.80 1433873_s_at Pcnt BG069597 0.91 1.46 1438081_at Mcc BB794635 0.85 1.53 1439485_at Zfp608 AV222442 0.91 1.43 1438951_x_at Nup54 BB168451 0.85 0.70 1449536_at Kcnn1 NM_032397 0.91 0.58 1429556_at 2610024B07Rik BM214769 0.85 1.59 1450097_s_at Gna12 BF302166 0.91 1.56 1437498_at Klf9 BB327336 0.85 0.51 1460121_at 9630010G10Rik BI076710 0.91 0.64 1460333_at Ddx59 BB829482 0.85 0.63 1424638_at Cdkn1a AK007630 0.91 0.57 1443246_at Fastkd2 BB476363 0.85 0.68 1434085_at Zfp523 AV136654 0.91 1.41 1457512_at BB167650 0.85 0.63 1441173_at 4930451G09Rik AV352442 0.91 0.69 1434596_at BB194318 0.85 1.56 1437554_at Plec1 BM232239 0.91 1.62 1419606_a_at Tnnt1 NM_011618 0.84 0.41 1455991_at Ccbl2 BG094881 0.91 0.67 1430019_a_at Hnrpa1 AK007802 0.84 0.70 1459882_at Asf1a AV312905 0.91 0.65 1422615_at Map4k4 NM_008696 0.84 1.58 1416199_at Kifc3 NM_010631 0.91 1.47 1447344_at AI447264 0.84 0.71 1458839_at Exoc8 BB032637 0.90 1.57 1440965_at Pigl AW018348 0.84 0.59 1439038_at 9130227C08Rik BF455240 0.90 0.59 1419048_at Pcnx BG073499 0.84 1.70 1420928_at St6gal1 BG075800 0.90 1.56 1418983_at Inadl AV287690 0.84 1.46 1423130_a_at Sfrs5 AW212917 0.90 0.59 1439060_s_at Wipi1 BI251603 0.84 0.69 1421478_a_at Zfp318 NM_021346 0.90 0.63 1450779_at Fabp7 NM_021272 0.84 0.68 1436524_at 4833438C02Rik AV252102 0.90 0.63 1419310_s_at Rfxank L43164 0.84 0.71 1416536_at Mum1 NM_023431 0.90 0.63 1429634_at Zfp580 AK005257 0.84 0.48 1435108_at Arhgap22 BG295243 0.90 1.42 1435753_a_at Nucks1 BM209748 0.84 0.63 1436278_at BM934468 0.90 0.64 1418369_at Prim1 J04620 0.84 0.67 1426597_s_at C79267 BG066466 0.90 1.53 1452600_at Taf6l AV280284 0.84 0.50 1418534_at Fzd2 BB371406 0.89 1.45 1455694_at Nbeal2 C77476 0.83 1.59 1437140_at Serf2 BB667186 0.83 0.64 1436245_at Usp20 AV272484 0.71 1.10 1441429_at Irs4 BB295945 0.83 2.12 1455124_at Trim68 AW493298 0.71 0.99 1454671_at Insig1 BB005488 0.83 0.69 1455563_at Ddx49 BM210028 0.71 0.94 1451042_a_at Mina AK013451 0.83 0.62 1457378_at Mrpl54 AW123022 0.71 1.23 1448602_at Pygm NM_011224 0.83 1.45 1419967_at Seh1l AW540070 0.70 0.82 1426015_s_at Asph AF302653 0.82 0.71 1441000_at EG237749 BB076832 0.70 0.75 1430135_at Dnase2a AK018651 0.81 0.67 1435152_at Leng8 AV024387 0.70 0.81 1454765_at Gtf3c3 AW549348 0.81 1.48 1431322_at Igsf3 AK019524 0.70 0.70 1422793_at Pafah1b2 BB539054 0.81 0.69 1418070_at Cdyl AF081260 0.70 1.27 1438189_s_at Rai16 BB492312 0.80 2.01 1443372_at BB417900 0.70 0.97 1422036_at Strn NM_011500 0.80 0.68 1445161_at Usp6nl BE952061 0.70 0.82 1415728_at Pabpn1 BG806432 0.80 0.65 1449062_at Khk BC013464 0.70 0.87 1433446_at Hmgcs1 BB705380 0.80 0.69 1439867_at Ubox5 BB079377 0.70 0.83 1443928_at Pscdbp AI462064 0.79 0.56 1422622_at Nos3 NM_008713 0.70 1.04 1434347_s_at Rufy2 BB827830 0.79 0.71 1429229_s_at 4930534B04Rik BE980134 0.70 0.90 1426589_at Gab3 BB037935 0.79 0.60 1443966_at C030046E11Rik BF466594 0.70 0.82 1424721_at Mfap3 BI661422 0.78 1.45 1429895_at 2310010G23Rik AK009271 0.70 0.88 1420146_at Tfb1m AI429207 0.78 0.70 1446041_at BB343268 0.70 0.75 1453518_at Pdzd11 AI429425 0.78 0.52 1424336_at Ppcdc BB329391 0.70 0.91 1434749_at BC067068 BB811762 0.77 0.67 1454727_at Afap1l1 BB106834 0.70 1.47 1439774_at Prrx1 BB051738 0.77 2.18 1458667_at 4930519N13Rik AV266695 0.70 0.64 1452121_at Fbxo22 BB756840 0.77 0.63 1425891_a_at Grtp1 AF329833 0.70 1.08 1444461_at Zmynd11 BB346871 0.77 0.52 1429671_at 2410018M08Rik AK010551 0.70 1.00 1452228_at Tbc1d23 AV209678 0.77 0.54 1433023_at 2310068G24Rik AK010109 0.70 0.98 1427149_at Plekha6 BB486127 0.77 1.52 1439047_s_at Recql BB258011 0.70 0.90 1453163_at Ppp1r12a BI653999 0.77 0.48 1445308_at Tssc1 BB082634 0.70 0.81 1435448_at Bcl2l11 BM120925 0.77 1.66 1436787_x_at Sec14l3 AV024133 0.69 0.65 1426226_at Dyrk1a U58497 0.76 0.54 1438573_at Rmi1 AV339477 0.69 0.93 1441993_at Ap3s2 BB762985 0.76 1.42 1453320_at 1700027A23Rik AK006407 0.69 0.69 1445564_at BE688513 0.75 1.45 1441106_at Zfand5 BB320444 0.69 1.09 1434166_at 9330151L19Rik BM935724 0.75 0.62 1423747_a_at Pdk1 BC027196 0.69 1.01 1457195_at Plekhm1 BB206527 0.74 0.67 1451692_at Tmco6 BC005637 0.69 0.95 1451809_s_at Rwdd3 AF439556 0.74 0.52 1424597_at ORF19 W34605 0.69 0.75 1434423_at Gulp1 BB138485 0.74 0.62 1441867_x_at 4930534B04Rik AI480494 0.69 0.71 1442046_at Shroom2 BM119167 0.74 0.67 1420044_at Nrd1 AI875733 0.69 0.81 1434695_at Dtl AV270035 0.74 1.86 1426230_at Sphk2 AK016616 0.69 0.71 1448700_at G0s2 NM_008059 0.74 1.57 1438852_x_at Mcm6 BB099487 0.69 0.88 1428684_at 1500001M20Rik BG065846 0.74 0.71 1424955_at Ccdc5 BC024400 0.68 0.75 1428825_at Nr6a1 AK007201 0.73 1.43 1433954_at 4632419I22Rik AV227569 0.68 1.17 1435079_at 5730406M06Rik BB767442 0.73 0.45 1434326_x_at Coro2b BB317923 0.68 0.87 1424839_a_at Nsun4 BC024628 0.73 0.70 1417920_at Amn NM_033603 0.68 0.57 1429796_at Kalrn AK008844 0.72 0.67 1418717_at Mrps25 AK004037 0.68 1.17 1434730_at AI854517 BB346879 0.72 0.70 1427838_at Tubb2a M28739 0.68 0.94 1449800_x_at Ph17 AI427892 0.71 0.66 1428704_at Zfp661 BB065039 0.68 1.03 1418984_at Inadl AV287690 0.71 1.81 1434591_at 4732460K03Rik BB004441 0.68 1.18 1428481_s_at Cdca8 AV307110 0.71 0.77 1445285_at Hrh3 AV381093 0.68 1.22 1443710_s_at Spats1 AW045373 0.71 1.04 1418739_at Sgk2 NM_013731 0.68 1.05 1452805_at D11Wsu47e AK017508 0.71 1.21 1455114_at Ccnu BB547482 0.68 0.69 1423854_a_at Rasl11b BC008101 0.71 0.86 1416429_a_at Cat NM_009804 0.68 0.87 1428115_a_at Rab2b AK005230 0.71 0.75 1433600_at Adra2a BB262415 0.68 1.01 1439645_at Adra2b BQ031032 0.71 0.91 1454840_at Mccc2 BB548059 0.68 0.65 1420059_at 2410166I05Rik AI480750 0.71 0.85 1444395_at Dixdc1 AW455436 0.68 1.04 1424742_AT Creb3 BG070002 0.71 0.88 1435691_at C630028N24Rik BB209207 0.68 0.80 1420287_at R75193 0.71 0.74 1436573_at Scrn3 BB434447 0.68 0.63 1454614_at 1810013D10Rik C87702 0.71 0.90 1415877_at Dpysl3 NM_009468 0.68 0.97 1458281_at Arsb BF018283 0.67 0.58 1422307_at NM_031400 0.62 0.77 1431279_s_at Ttll5 AK016145 0.67 0.97 1431578_at Wbp2nl AK015863 0.62 1.00 1421209_s_at Ikbkg NM_010547 0.67 0.95 1430997_at Cd47 AI882525 0.62 0.76 1417325_at Btrc NM_009771 0.67 0.76 1441208_at Hdhd2 AW546142 0.62 1.10 1417752_at Coro1c NM_011779 0.67 1.03 1453607_at Mfap3l AV280494 0.61 1.54 1423690_s_at Gpsm1 BC026486 0.67 0.76 1452328_s_at Pja2 BF160731 0.61 0.80 1431341_at Ppp2cb BF143676 0.67 1.14 1447292_at Actr1b BB815306 0.61 1.01 1453061_at 2610018O07Rik AK011449 0.67 0.71 1458870_x_at Phr1 BG083658 0.61 1.48 1455048_at Igsf3 BB484576 0.67 0.91 1456760_at Centg3 BB342676 0.61 0.94 1428547_at Nt5e AV273591 0.67 0.88 1436332_at Hspb6 BB755506 0.60 0.90 1432391_at Ccdc21 AK010621 0.67 1.14 1436867_at Srl BG795043 0.60 0.72 1438606_a_at Clic4 BB814844 0.67 0.97 1416164_at Fbln5 NM_011812 0.60 0.63 1438733_at Zfp689 BF461546 0.67 1.01 1447544_at BB486335 0.60 1.06 1447278_at Cep164 BB822306 0.67 1.08 1421144_at Rpgrip1 NM_023879 0.60 0.50 1441919_x_at Crat BB515871 0.66 1.06 1439609_at BB536410 0.59 0.86 1423762_at Adck1 BC010539 0.66 0.81 1456641_at 1190007F08Rik AV225851 0.59 0.84 1459703_at D7Ertd443e BG067408 0.66 0.59 1443813_x_at Bckdk AV373435 0.59 0.78 1419069_at Rabgef1 BB280137 0.66 1.55 1443542_at BB767151 0.59 0.79 1434987_at Aldh2 AI462635 0.66 0.85 1420864_at Zfp161 NM_009547 0.59 0.86 1448246_at Hdac1 NM_008228 0.66 0.67 1418128_at Adcy6 NM_007405 0.58 1.65 1460665_a_at Cnot7 NM_011135 0.66 0.52 1433923_at Krt77 AV230775 0.58 0.78 1421786_at Ppp3r1 NM_024459 0.65 1.23 1420913_at Slco2a1 NM_033314 0.58 0.98 1427491_at Nsun6 BI108529 0.65 0.93 1437785_at Adamts9 AV364944 0.57 1.07 1429012_at Arhgef6 BM246754 0.65 0.67 1431206_at 5730601F06Rik BI110339 0.57 0.83 1440084_at AI663975 AV380966 0.65 0.60 1426658_x_at Phgdh L21027 0.57 0.81 1440742_at Epc1 BF019417 0.65 0.91 1417420_at Ccnd1 NM_007631 0.57 0.92 1434844_at Hexdc BF582632 0.65 1.34 1421036_at Npas2 BG070037 0.57 0.79 1443719_x_at Ddx42 AW763628 0.65 0.97 1430222_at 9130007G19Rik BB538672 0.55 0.63 1447851_x_at Atp10a BB487289 0.65 0.89 1445536_at AW061096 AW061096 0.55 0.86 1421986_at Eif4e2 AI449084 0.65 0.87 1449915_at Zfp202 NM_030713 0.55 0.79 1429295_s_at Trip13 AK010336 0.65 0.58 1456784_at ″OTTMUSG 1420192_at D16Bwg1494e N28171 0.65 0.83 00000015762″ BB011034 0.55 0.73 1424451_at Acaa1b BC019882 0.64 0.53 1444304_at A330102K23Rik BE980520 0.54 0.89 1443918_at 2700050L05Rik BG068962 0.64 0.86 1438679_at Trim8 AI843272 0.54 0.70 1426313_at Bre BE984258 0.64 0.49 1456515_s_at Tcfl5 AV044715 0.54 0.96 1439391_at 2810002I04Rik BB559552 0.64 0.78 1455255_at 4833420G11Rik AV250006 0.54 1.12 1446769_at 2810439F02Rik AV028075 0.64 1.01 1430582_at Shprh BB496854 0.54 0.76 1438979_s_at 1700029I15Rik AV043667 0.64 1.20 1452473_at Prr15 AJ132433 0.53 0.60 1451434_s_at Gpatch8 BC019948 0.64 0.88 1447580_at AI835197 0.53 1.41 1420130_s_at D10Wsu52e AA409545 0.64 0.86 1427148_at Pja2 BF160731 0.52 0.75 1445221_at BB451452 0.64 0.96 1418019_at Cpd NM_007754 0.51 0.25 1441380_at 2810439F02Rik BB044461 0.63 0.81 1454429_at 5830462O15Rik AK018030 0.51 0.56 1455805_x_at Ccdc22 BB165451 0.63 0.82 1456799_at E130110O22Rik BB540910 0.51 0.85 1456674_at E130016E03Rik BB772877 0.63 0.77 1417479_at Ppp2r3c AV367559 0.51 0.82 1429698_at LOC208595 AK016498 0.63 0.82 1427963_s_at Rdh9 BE979765 0.50 0.69 1448818_at Wnt5a BC018425 0.63 1.04 1420889_at Hccs BB129992 0.49 1.12 1429819_at Nmnat1 AK017631 0.63 1.06 1459766_x_at Sf1 BB055869 0.47 0.93 1451602_at Snx6 BC025911 0.63 0.81 1431216_s_at Dnajc6 BI730538 0.41 0.79 1421141_a_at Foxp1 BG962849 0.63 1.05 1447773_x_at 6330409D20Rik BB308792 0.41 1.22 1428665_at Pfn4 BQ174081 0.63 0.73 1455075_at Pigv BB321057 0.62 1.10 1432589_at Plcg1 BF166706 0.62 0.83 1447870_x_at 1110002E22Rik BB099116 0.62 0.61 Probe = Affymetrix probe identification; Symbol = official gene symbol; FCH D1 = fold change in striatonigral (D1) cells; FCH D2 = fold change in striatopallidal (D2) cells.

Statistically over-represented Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms associated with genes whose expression was changed upon acute or chronic cocaine administration were searched (tables below). Amongst the most GO biological processes altered in D1-expressing striatonigral neurons upon chronic cocaine administration was the gamma-aminobutyric acid (GABA) signaling pathway (Gabrb3, Gabra1, Cacnb4, and Gabra4). This finding is relevant in light of positron emission tomography (PET) studies that have documented an enhanced sensitivity to the benzodiazepine lorazepam (binds GABA receptors) among chronic cocaine abusers (N. D. Volkow et al., Am J Psychiatry 155, 200-6 (February, 1998)).

TABLE 17 Top 1,000 striatonigral (D1) cell translated mRNAs enriched when compared to a reference sample ProbeSymbol FEN Genbank GeneSymbol ProbeSymbol FEN Genbank GeneSymbol 1452217_at 112.5 BE570050 Ahnak 1435749_at 19.85 AW911807 Gda 1426814_at 100.6 BM248309 AU024582 1423530_at 19.32 BB320288 Stk32c 1418357_at 71.38 NM_008241 Foxg1 1417356_at 19.31 AB003040 Peg3 1419231_s_at 70.83 NM_010661 Krt1-12 1425870_a_at 19.26 AF439339 Kcnip2 1427017_at 70.71 BB104560 Satb2 1427358_a_at 18.94 BC026671 Dapk1 1435564_at 68.33 BB547893 C230078M08Rik 1435615_at 18.72 BB277790 Zfp365 1457132_at 57.88 BF456117 1455034_at 18.64 BB703394 Nr4a2 1458499_at 47.5 AW123977 Pde10a 1444681_at 18.62 BB749686 D14Ertd171e 1443287_at 47.05 BB555669 Gm1337 1434248_at 18.36 BM243756 Prkch 1422079_at 45.93 NM_008856 Prkch 1427343_at 18.15 BC026377 Rasd2 1438022_at 45.81 BQ266518 Rab11fip3 1450071_at 18.15 BG694892 Ash1l 1457424_at 44.35 BB760085 Eya1 1451280_at 17.94 BB159263 MGI: 107562 1426815_s_at 44.33 BM248309 AU024582 1436329_at 17.56 AV346607 Egr3 1436268_at 40.01 AI841578 Ddn 1434653_at 17.09 AV026976 Ptk2b 1456051_at 38.14 BB282271 Drd1a 1434115_at 16.95 BQ176681 1416711_at 36.16 NM_009322 Tbr1 1424507_at 16.92 BC011277 Rin1 1420437_at 34.39 NM_008324 Indo 1452176_at 16.72 BB292874 Nup153 1455629_at 33.34 BE957273 Drd1a 1453129_a_at 16.55 AK004813 Rgs12 1454867_at 32.24 BB234631 Mn1 1427683_at 16.48 X06746 Egr2 1434917_at 31.38 BQ173923 Cobl 1418782_at 16.46 NM_009107 Rxrg 1428642_at 31.19 AK018094 Slc35d3 1452966_at 16.43 AK020296 9130430L19Rik 1423117_at 30.07 BB837171 Pum1 1457341_at 16.07 BE991676 1460325_at 29.16 BB837171 Pum1 1425503_at 16.07 AB037596 Gcnt2 1416776_at 24.48 NM_016669 Crym 1423171_at 15.52 BE947345 Gpr88 1424606_at 23.37 BC024854 Cplx3 1430306_a_at 15.47 AK004157 Atp6v1c2 1450339_a_at 22.89 NM_021399 Bcl11b 1456283_at 15.47 AV346211 Neto1 1449425_at 22.68 BCO26373 Wnt2 1427682_a_at 15.33 X06746 Egr2 1423802_at 22.57 BC017634 Camkv 1422034_a_at 15.24 NM_023128 Palm 1437698_at 22.46 AV370579 Myo5b 1449420_at 15.22 NM_008800 Pde1b 1427807_at 22.23 BC017159 4930448N21Rik 1450072_at 14.84 BG694892 Ash1l 1422720_at 21.52 BQ176915 Isl1 1435227_at 14.76 BM117007 1435083_at 21.47 BI155559 Ctxn1 1455200_at 14.73 BB818370 Pak6 1439854_at 21.22 BQ175572 AI838259 1433566_at 14.65 BB381618 Rasl10b 1450042_at 21.2 BB322201 Arx 1456054_a_at 14.47 BB314559 Pum1 1425756_at 21.04 AF425643 Rab40b 1435366_at 14.24 BB486367 D430042O09Rik 1436650_at 20.92 AV241894 Filip1 1426980_s_at 14.23 BC006054 E130012A19Rik 1436275_at 20.82 AW490636 Kcnip2 1424248_at 14.1 BB159263 MGI: 107562 1427975_at 20.66 AK008807 2210403B10Rik 1416953_at 13.95 NM_010217 Ctgf 1436566_at 20.43 AV364488 Rab40b 1433701_at 13.94 BB371430 Mpped1 1448364_at 13.87 U95826 Ccng2 1435296_at 10.95 AV349563 Adra2c 1418619_at 13.83 NM_008319 Icam5 1434521_at 10.89 BB148972 Rfxdc2 1450750_a_at 13.58 NM_013613 Nr4a2 1438665_at 10.88 BF456582 Smpd3 1450061_at 13.55 BM120053 Enc1 1444978_at 10.86 BM223267 1454043_a_at 13.52 AK015412 Kcnab1 1451331_at 10.75 BC026568 Ppp1r1b 1450723_at 13.4 BQ176915 Isl1 1422710_a_at 10.73 NM_021415 Cacna1h 1435748_at 13.37 AW911807 Gda 1430013_at 10.7 BE993118 2210008F06Rik 1425479_at 13.34 BF160651 Smyd5 1422902_s_at 10.63 NM_023799 Mgea5 1452202_at 13.28 BG069616 Pde2a 1422779_at 10.57 NM_021491 Smpd3 1432646_a_at 13.21 BE859789 2900097C17Rik 1428781_at 10.54 BI452905 1110014F24Rik 1452974_at 13.21 AK017551 Nol8 1460136_at 10.52 AI462839 1460038_at 13.11 BG065255 Pou3f1 1416691_at 10.51 NM_019581 Gtpbp2 1425130_a_at 13.06 U28216 Ptpn5 1450143_at 10.51 BB354696 Rasgrp1 1458342_at 13.01 BB313069 1435741_at 10.46 BB312125 Pde8b 1455190_at 12.97 BM114283 Gng7 1420666_at 10.35 BM117900 Doc2b 1436066_at 12.96 BI965477 Kalrn 1437125_at 10.24 BB476448 Camlaa 1428951_at 12.89 AK017551 Nol8 1454768_at 10.24 AV337635 Kcnf1 1436408_at 12.84 BE946298 1420978_at 10.23 NM_010938 Nrf1 1454906_at 12.83 BB266455 Rarb 1450227_at 10.22 BM199504 Ankrd6 1417560_at 12.81 BB478992 Sfxn1 1447359_at 10.22 AI326876 LOC381955 1440204_at 12.8 AW494150 3110039M20Rik 1436092_at 10.21 BB336256 1424037_at 12.77 BC027291 Itpka 1448327_at 10.11 NM_033268 Actn2 1422230_s_at 12.67 NM_007812 Cyp2a4; Cyp2a5 1420545_a_at 10.1 NM_029716 Chn1 1429422_at 12.57 AK016788 4933412E12Rik 1418691_at 10.03 NM_011268 Rgs9 1436532_at 12.51 BB326709 Dcamkl3 1423756_s_at 10.02 BC019836 Igibp4 1431936_a_at 12.33 AK009828 Neu2 1460343_at 10.02 AF401228 Neurl 1452298_a_at 12.18 AW546331 Myo5b 1421028_a_at 10.01 AI595932 Mef2c 1422153_a_at 12.11 NM_026853 Asb11 1429089_s_at 9.93 BG063749 2900026A02Rik 1427931_s_at 12.09 BG063905 Pdxk 1418015_at 9.916 BI689507 Pum2 1433627_at 12.04 AW546839 Sec23ip 1428950_s_at 9.758 AK017551 Nol8 1417279_at 11.94 NM_010585 Itpr1 1419033_at 9.756 AW556821 2610018G03Rik 1428139_at 11.9 AK015988 4930538D17Rik 1452879_at 9.72 AI848603 Synpo2 1450213_at 11.85 NM_013875 Pde7b 1441368_at 9.671 BB102769 1439552_at 11.83 BE985618 Trio 1447326_s_at 9.625 AW122925 Zfp261 1449634_a_at 11.74 AW046296 C030032C09Rik 1455564_at 9.621 BQ176236 Bcr 1438355_at 11.68 AI414870 1455620_at 9.572 BQ177219 1417355_at 11.64 AB003040 Peg3 1452507_at 9.568 AF022078 Dlx6 1456640_at 11.46 AW910872 Sh3rf2 1415904_at 9.554 BC003305 Lpl 1454721_at 11.44 AV257687 1110018G07Rik 1451507_at 9.554 BB280300 Mef2c 1435780_at 11.44 BG966595 Psd 1428338_at 9.553 AK019166 2610039E05Rik 1418047_at 11.42 NM_009717 Neurod6 1421353_at 9.538 NM_013875 Pde7b 1421176_at 11.39 BB354696 Rasgrp1 1419470_at 9.495 B1713933 Gnb4 1450026_a_at 11.37 AV306734 B3gnt1 1417114_at 9.466 AF163665 Gdl 1457311_at 11.36 AW490258 Camlaa 1448990_a_at 9.433 AI255256 Myo1b 1451604_a_at 11.23 BC014291 Acyrl1 1458406_at 9.404 BG144063 1416437_a_at 11.21 AF262046 Mapk8ip3 1430521_s_at 9.38 AW548480 Cpne8 1451726_at 11.18 BC020019 Mtmr6 1434025_at 9.365 BG069607 1440534_at 11.16 BB177862 1422621_at 9.363 BM507707 Ranbp2 1451808_at 11.16 U11075 Kcnj4 1423202_a_at 9.355 U22016 Ncor1 1434295_at 11.16 BE691356 Rasgrp1 1422165_at 9.336 X66603 Pou3f4 1457143_at 11.12 BB322292 Gli3 1433815_at 9.334 AV290082 MGI: 1923321 1416456_a_at 11.11 BC011134 Chia 1423489_at 9.324 BC021914 Mmd 1442166_at 11.08 BB273427 Cpne5 1460567_at 9.318 BB148972 Rfxdc2 1448366_at 11.05 NM_016801 Stx1a 1446492_at 9.312 BB396896 C630028F04Rik 1421739_a_at 11.05 NM_010768 Matk 1420580_at 9.273 NM_026249 4930429B21Rik 1427045_at 11.03 AI849322 Synpo 1417804_at 9.21 NM_011242 LOC381240 1457052_at 9.179 BG064867 AW536275 1427344_s_at 8.237 BC026377 Rasd2 1460555_at 9.176 BM242294 6330500D04Rik 1437929_at 8.236 AV306847 Dact2 1417680_at 9.153 NM_008419 Kcna5 1421817_at 8.23 AK019177 Gsr 1454656_at 9.123 AV271736 Spata13 1443612_at 8.216 AW123199 1458407_s_at 9.093 BG144063 1428852_at 8.213 AK018140 Dock3 1454752_at 9.081 AV307961 AI606861 1423561_at 8.172 AI838010 Nell2 1452368_at 9.043 AI853148 Bcr 1417440_at 8.166 NM_033566 Arid1a 1453273_at 9.038 AV142265 Kcnv1 1440849_at 8.044 AV327187 6330417G04Rik 1455701_at 9.019 BI965517 Snx26 1437918_at 8.028 AV374644 4930539E08Rik 1452476_at 8.997 W41214 Cacnb2 1433583_at 8.025 AV327248 Zfp365 1418169_at 8.988 BB223737 Zcchc14 1429285_at 8.014 AK009343 Serpina9 1423285_at 8.971 BB731671 Coch 1436734_at 8.003 BB523550 E130309F12Rik 1455447_at 8.93 BM116882 D430019H16Rik 1438985_x_at 8 BB158937 Otud5 1436998_at 8.924 BB428991 Ankrd43 1418017_at 7.974 BI689507 Pum2 1455220_at 8.9 BB353860 Frat2 1435435_at 7.965 BB357580 Cttnbp2 1419224_at 8.894 NM_033567 Cecr6 1455885_at 7.965 AV238106 6530401C20Rik 1442234_at 8.872 BB770422 Chst2 1423544_at 7.964 BB188812 Ptpn5 1439557_s_at 8.849 BB097063 Ldb2 1421446_at 7.962 NM_011102 Prkcc 1457729_at 8.819 AW492805 1454742_at 7.942 BB003229 Rasgef1b 1448978_at 8.804 NM_019867 Ngef 1419389_at 7.941 BQ180352 Pde10a 1419221_a_at 8.803 NM_016758 Rgs14 1426518_at 7.939 AV333951 Tubgcp5 1456515_s_at 8.774 AV044715 Tcfl5 1423636_at 7.931 AK017259 Wdr31 1455272_at 8.747 BB429139 Grm5 1433767_at 7.931 AV257687 1110018G07Rik 1418373_at 8.673 NM_018870 Pgam2 1416562_at 7.91 AF326547 Gad1 1422787_at 8.672 NM_019873 Fkbpl 1427450_x_at 7.907 BI080370 Myo1b 1437091_at 8.631 AV323885 Accn4 1454845_x_at 7.905 AW049955 Gpr24 1451517_at 8.627 AF420001 Rhobtb2 1420388_at 7.905 NM_008939 Prss12 1417462_at 8.62 NM_007598 Cap1 1422901_at 7.896 NM_023799 Mgea5 1455361_at 8.612 AW493391 Dgkb 1416804_at 7.845 NM_053252 LOC114601 1416783_at 8.595 NM_009311 Tac1 1416350_at 7.826 NM_078477 Klf16 1428872_at 8.573 AW495537 4121402D02Rik 1440148_at 7.825 AI852874 Gpr6 1418881_at 8.568 NM_054095 Efcbp2 1424796_at 7.809 BC024705 1700054N08Rik 1431164_at 8.563 AK017818 Rragd 1457440_at 7.808 BB451927 Sstr4 1455754_at 8.54 BQ043839 Lmo3 1427618_at 7.801 BQ176417 Cdh9 1439767_at 8.526 BB130159 Dlgap2 1447863_s_at 7.794 BB322941 Nr4a2 1432378_at 8.526 AK021043 C030004G16Rik 1428535_at 7.775 AK004276 9430020K01Rik 1422018_at 8.509 NM_010437 Hivep2 1431394_a_at 7.754 AK014938 Lrrk2 1441894_s_at 8.487 BB071890 Grasp 1430675_at 7.733 BM932606 2900055J20Rik 1421142_s_at 8.473 BG962849 Foxp1 1460206_at 7.728 NM_019518 Grasp 1423551_at 8.43 BB776961 Cdh13 1423186_at 7.703 BM228957 Tiam2 1420961_a_at 8.428 NM_028582 Ivns1abp 1448807_at 7.7 NM_133849 Hrh3 1455683_a_at 8.423 BB451404 Tbc1d8 1456401_at 7.693 BB078175 Cacnb2 1434077_at 8.407 AV222037 Wdr37 1450690_at 7.69 BM507707 Ranbp2 1436312_at 8.405 AV317621 Zfpn1a1 1434429_at 7.684 AV348245 Syt16 1445539_at 8.395 BE687857 Pde7b 1451506_at 7.667 BB280300 Mef2c 1435221_at 8.388 BM220880 Foxp1 1427426_at 7.619 AV362204 Kcnq5 1441733_s_at 8.387 C88147 Nup153 1445691_at 7.613 AW121426 Chn1 1426915_at 8.387 BC021490 Dapk1 1434695_at 7.594 AV270035 Dtl 1416286_at 8.354 NM_009062 Rgs4 1425833_a_at 7.582 AF326551 Hpca 1418714_at 8.352 NM_008748 Dusp8 1456336_at 7.561 BB182912 A330102K23Rik 1427005_at 8.339 BM234765 Plk2 1437865_at 7.55 AW546433 Spata13 1453782_at 8.33 BI737125 3021401C12Rik 1435605_at 7.538 BB125424 Actr3b 1421101_a_at 8.319 NM_010698 Ldb2 1422678_at 7.531 AK002443 Dgat2 1416266_at 8.283 AF026537 Pdyn 1449472_at 7.521 NM_008151 Gpr12 1423376_a_at 8.251 AV341904 Dok4 1456786_at 7.493 BB097063 Ldb2 1429617_at 8.246 BM119209 Cyld 1429741_at 7.479 AV142265 Kcnv1 1442434_at 7.465 BM195829 D8Ertd82e 1441963_at 6.895 BB427703 MGI: 2656976 1442113_at 7.463 BB183350 5330417C22Rik 1448989_a_at 6.888 AI255256 Myo1b 1448656_at 7.462 NM_007581 Cacnb3 1421027_a_at 6.878 AI595932 Mef2c 1423012_at 7.457 NM_018801 Syt7 1455296_at 6.854 BE952286 Adcy5 1446498_at 7.409 BB551879 E230031K19 1435666_at 6.846 AW553439 1422068_at 7.389 NM_011141 Pou3f1 1435071_at 6.829 AV327165 Zfyve1 1417129_a_at 7.369 U68384 Mrg1 1439548_at 6.82 BB390705 Rap2b 1423344_at 7.368 AK010968 Epor 1429104_at 6.817 AK012581 0610025L06Rik 1454877_at 7.333 BQ174721 Sertad4 1437460_x_at 6.805 BB264363 Rin1 1426585_s_at 7.326 BM209765 Mapk1 1455758_at 6.804 BM215011 1426901_s_at 7.32 BB657856 Camta2 1425929_a_at 6.804 AF249668 Rnf14 1423240_at 7.31 BG868120 Src 1457919_at 6.801 BB446049 D030054H15Rik 1420981_a_at 7.304 NM_010723 Lmo4 1443694_at 6.8 BB794177 Rgs20 1431074_a_at 7.298 BI654600 Pitpnc1 1417090_at 6.799 NM_009037 Rcn1 1438889_at 7.294 BB183456 1452964_at 6.774 AK016577 4932702F08Rik 1418847_at 7.268 NM_009705 Arg2 1431230_a_at 6.748 Z74630 Btbd9 1436733_at 7.257 BB523550 E130309F12Rik 1439569_at 6.734 AV291031 Gpr83 1424201_a_at 7.248 AW537349 Seh1l 1439866_at 6.733 BQ173950 Parc 1457979_at 7.244 BM938335 1447728_x_at 6.677 BB718260 Hspa9a 1421141_a_at 7.232 BG962849 Foxp1 1435210_s_at 6.675 BQ174936 Snph 1450655_at 7.231 AA214868 Herd1 1419542_at 6.653 NM_010021 Dazl 1437586_at 7.22 BB756908 Cnot4 1430617_at 6.632 BB238604 Oip5 1434008_at 7.201 BE993937 Scn4b 1419137_at 6.631 NM_021423 Shank3 1419696_at 7.198 NM_013488 Cd4 1420530_at 6.63 AW553317 Neud4 1417341_a_at 7.194 NM_025800 Ppp1r2 1447301_at 6.625 BG228875 Gm258 1434458_at 7.182 BB444134 Fst 1418144_a_at 6.621 NM_008847 Pip5k1b 1438784_at 7.162 BB329234 1422683_at 6.62 NM_022986 Irak1bp1 1421789_s_at 7.149 NM_007478 Arf3 1455056_at 6.612 BM231903 Lmo7 1452624_at 7.147 BM900077 Lrrtm1 1421346_a_at 6.608 NM_009320 Slc6a6 1450835_a_at 7.147 NM_020014 Gfra4 1434359_at 6.607 AV329070 6330500D04Rik 1419390_at 7.143 BQ180352 Pde10a 1423640_at 6.584 BC026512 Synpr 1429316_at 7.138 AK018120 Rasgef1a 1418080_at 6.581 AW125856 B4galt2 1436381_at 7.133 BQ175774 Dlgap3 1421140_a_at 6.58 BG962849 Foxp1 1433969_at 7.121 AU067824 AU067824 1451285_at 6.58 AF224264 Fus 1426009_a_at 7.119 BC003763 Pip5k1b 1451236_at 6.57 BC026463 Rerg 1434090_at 7.098 BB037094 4930429A22Rik 1435043_at 6.569 BB794831 Plcb1 1437018_at 7.083 BG072348 Pnma2 1434029_at 6.567 AW545676 LOC545794 1430127_a_at 7.067 AK007904 Ccnd2 1443888_at 6.535 BB426608 1415743_at 7.065 NM_010412 Hdac5 1430037_at 6.531 AK017836 Snx27 1452940_x_at 7.049 AI595920 Pitpnc1 1447178_at 6.529 BB173057 1456280_at 7.049 BG067086 Clspn 1420857_at 6.525 NM_133737 Lancl2 1416398_at 7.036 BB474887 Mesdc1 1460447_at 6.52 AK019372 3000003F02Rik 1423231_at 7.034 AK002933 Nrgn 1420146_at 6.514 AI429207 Tiam2 1451270_at 7.031 BC020036 Dusp18 1419216_at 6.506 NM_009734 Azi1 1452467_at 7.029 BQ042988 Mmab 1460262_a_at 6.492 NM_026091 1700037H04Rik 1438799_at 7.028 AV338180 Dlx6os1 1421340_at 6.483 NM_008580 Map3k5 1429637_at 7.024 AK008987 2210419I08Rik 1415976_a_at 6.468 AU080787 Carhsp1 1432073_at 7.021 AK007198 1700113I22Rik 1419066_at 6.46 NM_030244 Ier5l 1435558_at 7.019 BB351248 Bai2 1422706_at 6.45 AV370981 Tmepai 1436738_at 6.98 AV094878 AI449441 1459679_s_at 6.45 AA406997 Myo1b 1456785_at 6.96 BQ175889 Crsp2 1438751_at 6.44 BB736474 Slc30a10 1435808_at 6.954 BB174377 A230051G13Rik 1454960_at 6.43 BI646741 Smad3 1439855_at 6.931 AV341449 BC023818 1425510_at 6.43 BM213279 Mark1 1417524_at 6.918 NM_009920 Cnih2 1429224_at 6.419 AK017476 Pnma1 1435553_at 6.896 AV376136 Pdzk3 1449468_at 6.412 NM_012028 St6galnac5 1431920_a_at 6.896 AK008082 2010004A03Rik 1450748_at 6.41 NM_021491 Smpd3 1431422_a_at 6.399 AK009744 Dusp14 1418535_at 6.023 NM_016846 Rgl1 1423532_at 6.374 AI850285 Rnf44 1425249_a_at 6.007 AB000828 Tyro3 1422335_at 6.373 NM_007418 Adra2c 1453886_a_at 6.005 AK017037 Slc25a26 1422527_at 6.373 NM_010386 H2-DMa 1421897_at 6 AI385733 Elk1 1421191_s_at 6.372 NM_053187 Gopc 1455178_at 6 BB496468 Rutbc1 1434762_at 6.358 BF457736 A730041O15Rik 1436403_at 5.998 BB452429 BC025575 1420377_at 6.336 BG071333 St8sia2 1420610_at 5.979 AV024339 Prkacb 1437001_at 6.333 BQ173949 Gsk3b 1416996_at 5.979 BC005421 Tbe1d8 1435763_at 6.322 BI218684 Tbe1d16 1433451_at 5.967 BB177836 Cdk5r1 1434153_at 6.316 BI408715 Shb 1438664_at 5.966 BB216074 Prkar2b 1427565_a_at 6.309 AF213387 Abcc5 1417461_at 5.957 NM_007598 Cap1 1427468_at 6.307 M81483 Ppp3cb 1459051_at 5.956 BB781615 6530418L21Rik 1440940_at 6.305 BE949510 Cacnb1 1426450_at 5.955 BM207017 Plel2 1449956_at 6.301 NM_011104 Prkce 1455301_at 5.95 BG064092 BQ952480 1436100_at 6.298 AV347995 Sh2d5 1423973_a_at 5.949 BC024935 Arf3 1420871_at 6.293 BF472806 Gucy1b3 1417812_a_at 5.948 NM_008484 Lamb3 1455225_at 6.293 AV237615 Syne1 1423694_at 5.934 BC006935 Ketd10 1448709_at 6.292 NM_033566 Arid1a 1417612_at 5.933 BF147705 Ier5 1438224_at 6.287 BE572265 Zswim5 1425680_a_at 5.92 AF110396 Btrc 1448023_at 6.278 BB662566 Kalrn 1448063_at 5.918 AW226526 Iqsec2 1426024_a_at 6.278 AF187147 Dbn1 1448414_at 5.911 NM_011232 Rad1 1435500_at 6.266 BB270778 Rab26 1423478_at 5.909 BF660388 Prkeb1 1451751_at 6.258 AF335325 Ddit4l 1449921_s_at 5.903 NM_009947 Cpne6 1453836_a_at 6.25 AK006949 Mgll 1429438_at 5.888 AV318805 Bcor 1417559_at 6.244 BB478992 Sfxn1 1457077_at 5.875 BB306048 1456509_at 6.236 BB382807 1110032F04Rik 1427299_at 5.87 BE376079 Rps6ka3 1440361_at 6.23 BB272510 1428892_at 5.869 AK004331 Ppil1 1425465_a_at 6.225 AV317107 Senp2 1449164_at 5.866 BC021637 Cd68 1460279_a_at 6.222 AF043219 Gtf2i 1434763_at 5.864 BF457736 A730041O15Rik 1440207_at 6.219 BI155142 AI505034 1435265_at 5.861 BF466929 1444139_at 6.189 BG797099 Ddit4l 1436754_at 5.86 BB360523 AI839735 1421860_at 6.171 BG065300 Clstn1 1433642_at 5.857 BB384173 Arfrp2 1458443_at 6.17 BE986758 Mect1 1434154_at 5.83 BQ177107 Kctd13 1448374_at 6.167 AK005130 Med28 1449158_at 5.824 NM_010607 Kcnk2 1454762_at 6.164 BM932447 Xkrx 1441867_x_at 5.82 AI480494 4930534B04Rik 1451908_a_at 6.154 BC005766 Sec14l1 1429228_at 5.818 BE980134 4930534B04Rik 1429718_at 6.15 BB782729 Slitrk5 1421116_a_at 5.811 NM_024226 Rtn4 1451696_at 6.146 BC004695 Zfp64 1426416_a_at 5.809 AV216410 Yipf4 1449957_at 6.14 NM_007955 Ptprv 1449241_at 5.803 NM_053105 Klhl1 1439041_at 6.128 BM239325 Slc39a10 1453093_at 5.801 AK018593 Rasgef1c 1433471_at 6.127 AI323642 Tcf7 1417305_at 5.798 NM_007463 Apeg1 1430640_a_at 6.126 BI695530 Prkar2b 1441065_at 5.787 BB305753 1429111_at 6.102 AV270892 Tln2 1442754_at 5.778 BE692283 C030013G03Rik 1421768_a_at 6.092 NM_011982 Homer1 1440711_at 5.777 BB426320 C630001G18Rik 1421000_at 6.085 A1448404 Cnot4 1418263_at 5.774 BC024852 Ddx25 1460718_s_at 6.076 AF192558 Mtch1 1421175_at 5.771 NM_008666 Myt1l 1434775_at 6.06 AW543460 Pard3 1431056_a_at 5.77 AK017272 Lpl 1435218_at 6.058 BI134758 Rasgef1a 1457702_at 5.766 BB284435 Gpr12 1452861_at 6.049 AK008485 2010300C02Rik 1422407_s_at 5.761 NM_008284 Hras1 1454609_x_at 6.047 BB770958 6430527G18Rik 1422208_a_at 5.761 BC016135 Gnb5 1457651_x_at 6.046 BB270375 Rem2 1455387_at 5.76 AV112972 1110001M19Rik 1438068_at 6.042 BB251859 1455191_x_at 5.747 BB822856 Pip5k1b 1452380_at 6.026 BB075797 Epha7 1440918_at 5.735 AW495511 Rasgrf2 1428184_at 6.025 BB348639 3110035E14Rik 1418982_at 5.731 BC011118 Cebpa 1425466_at 6.025 AV317107 Senp2 1433988_s_at 5.729 BG075755 C230098O21Rik 1426012_a_at 6.024 BC010499 2610301G19Rik 1435392_at 5.726 BB277182 Wdr17 1448842_at 5.714 NM_033037 Cdo1 1431749_a_at 5.467 AK013548 Rasgrp1 1426339_at 5.713 AB060081 Ak5 1434902_at 5.456 BB163668 Rnf157 1452917_at 5.709 AK011489 Rfc5 1423488_at 5.453 BC021914 Mmd 1427063_at 5.709 AK017241 5330417C22Rik 1452718_at 5.452 BG073486 Edd1 1436005_at 5.689 BQ174136 Sfrs14 1448598_at 5.449 NM_011846 Mmp17 1447017_at 5.687 BE956696 1436142_at 5.446 BM218877 3526401B18Rik 1426108_s_at 5.685 AY094172 Cacnb1 1424047_at 5.427 BC016218 Dera 1451008_at 5.683 BB360510 St8sia3 1451483_s_at 5.421 BC024705 1700054N08Rik 1424899_at 5.68 BC005737 Nmnat3 1424132_at 5.42 BC011083 Hras1 1428527_at 5.679 AK011015 Snx7 1454784_at 5.419 AV340742 Hs3st2 1415985_at 5.679 NM_133953 Sf3b3 1454835_at 5.413 AV340515 Epm2aip1 1448468_a_at 5.676 AF033003 Kcnab1 1448724_at 5.409 NM_009895 Cish 1418592_at 5.675 NM_021422 Dnaja4 1451098_at 5.406 BC010524 Pcoln3 1416982_at 5.671 AI462296 Foxo1 1418784_at 5.39 NM_008168 Grik5 1433460_at 5.669 BB329157 Ttc7b 1449470_at 5.388 NM_010053 Dlx1 1420496_at 5.666 NM_021489 F12 1427082_at 5.383 AK014586 4632417N05Rik 1444661_at 5.663 BB247791 Gpr26 1455085_at 5.379 BI526033 1700086L19Rik 1448546_at 5.651 BB703307 Rassf3 1455033_at 5.363 BB325849 B430201A12Rik 1447707_s_at 5.649 BB367174 Pde2a 1426241_a_at 5.356 AB030906 Scmh1 1418743_a_at 5.647 NM_021344 MGI: 1930803 1456755_at 5.353 AI467545 2310001H13Rik 1415834_at 5.646 NM_026268 Dusp6 1420718_at 5.352 NM_011856 Odz2 1448747_at 5.636 AF441120 Fbxo32 1457632_s_at 5.35 BB207647 Mrg1 1417192_at 5.631 NM_138599 Tomm70a 1427579_at 5.348 BE653576 Rhbdl4 1416754_at 5.63 NM_008923 Prkar1b 1423228_at 5.347 BG066773 B4galt6 1425617_at 5.626 U91922 Dhx9 1451254_at 5.344 AF367244 Ikbkap 1417522_at 5.606 AF441120 Fbxo32 1416242_at 5.343 NM_026167 Klhl13 1416770_at 5.604 NM_021537 Stk25 1425679_a_at 5.34 AF109769 Mapk8ip1 1421498_a_at 5.595 NM_023450 2010204K13Rik 1439611_at 5.307 BE947522 Chrm1 1441363_at 5.594 BQ174855 L00546381 1425059_at 5.306 BC022899 Hrmt1l6 1421037_at 5.594 BG070037 Npas2 1454985_at 5.303 BI872009 D030051N19Rik 1452278_a_at 5.59 BG922448 Hace1 1435128_at 5.301 BB429313 Baiap2 1421048_a_at 5.583 NM_023249 Ypel1 1434800_at 5.301 BQ175909 Sv2b 1422977_at 5.577 NM_010327 Gp1bb 1421959_s_at 5.298 AF458089 Adcy3 1430168_at 5.57 AK020589 Cstad 1434819_at 5.297 BB709312 St6gal2 1418789_at 5.563 NM_133742 Sntg2 1448687_at 5.291 NM_026125 C1qdc2 1438213_at 5.563 BB128962 A830018L16Rik 1455279_at 5.284 BG070552 Gm1060 1420965_a_at 5.546 BM120053 Enc1 1454832_at 5.275 BG228702 Phactr1 1451630_at 5.544 BC018513 Ttl 1418181_at 5.273 AK014601 Ptp4a3 1426852_x_at 5.539 X96585 Nov 1420872_at 5.27 BF472806 Gucy1b3 1456946_at 5.531 BE949337 4831416G18Rik 1423658_at 5.267 BCO23131 Sppl3 1426354_at 5.53 AK009033 Bap1 1455242_at 5.267 BM220880 Foxp1 1422293_a_at 5.527 NM_134112 Kctd1 1456444_at 5.265 BB126849 Fbxo41 1433724_at 5.519 BM235412 D15Ertd621e 1456930_at 5.264 BE989461 Camsap1 1449125_at 5.518 NM_025566 Tnfaip8l1 1450520_at 5.261 NM_019430 Cacng3 1421622_a_at 5.518 NM_019688 Rapgef4 1438417_at 5.256 BB809239 Pwwp2 1436216_s_at 5.516 BM234799 2610204M08Rik 1434594_at 5.255 BB497449 Dnahc1 1422705_at 5.515 AV370981 Tmepai 1424175_at 5.249 BC017689 Tef 1444723_at 5.506 BB049759 6530418L21Rik 1424028_at 5.249 BCO23107 5830457O10Rik 1441350_at 5.505 AV302620 Fgf3 1435367_at 5.244 BQ177154 Mapk4 1435204_at 5.501 BG065273 Hrmt1l4 1437485_at 5.236 BB098161 Nos1ap 1422799_at 5.501 AK019427 Bat2 1452506_a_at 5.231 BC011427 Ilkap 1427067_at 5.498 AJ404329 4933439F18Rik 1448553_at 5.228 NM_080728 Myh7 1437580_s_at 5.492 C77054 Nek2 1424389_at 5.228 BCO26743 Nupl1 1452057_at 5.49 BG801851 Actr1b 1451273_x_at 5.223 BCO25546 BC025546 1437405_a_at 5.489 BB787243 Igfbp4 1441928_x_at 5.223 BB139475 Ell 1439618_at 5.476 AI448308 Pde10a 1431717_at 5.222 AK014386 3526401B18Rik 1450910_at 5.213 AV261931 Cap2 1424481_s_at 5.042 BC027106 MGC38735 1419581_at 5.212 AI646416 Dlgh4 1422751_at 5.039 NM_011599 Tle1 1423395_at 5.202 BM021706 Tsnax 1423699_at 5.036 BC003900 D15Ertd785e 1456818_at 5.201 BB279083 Stk32a 1433805_at 5.034 BQ032637 Jak1 1448554_s_at 5.199 NM_080728 Myh6 1416087_at 5.03 NM_007457 Ap1s1 1441369_at 5.193 BB329116 1419522_at 5.027 NM_026021 Zmynd19 1448805_at 5.192 NM_009480 Usf1 1449494_at 5.026 AY026947 Rab3c 1435844_at 5.19 BB164247 A330009N23Rik 1425132_at 5.026 AF448840 Neto1 1443129_at 5.19 BB363699 1436648_at 5.025 BB251623 Nanos1 1421312_a_at 5.19 NM_010630 Kifc2 1443676_at 5.023 AV339935 Slc4a4 1428676_at 5.187 AK004939 Tmprss6 1451475_at 5.023 BC019530 Plxnd1 1454759_at 5.183 AV230461 Git1 1421225_a_at 5.022 NM_018760 Slc4a4 1455080_at 5.182 BB375209 Ppp1r16b 1420999_at 5.022 A1448404 Cnot4 1454092_a_at 5.182 AK017176 Gtf2h3 1441967_at 5.021 BB467791 BC023835 1438663_at 5.18 BF730196 Bat2d 1439662_at 5.018 BM124609 Homer1 1416050_a_at 5.176 NM_016741 Scarb1 1437750_at 5.015 BI133257 2310037P21Rik 1435618_at 5.174 BB473446 Pnma2 1426897_at 5.011 AV122997 Rcc2 1427523_at 5.168 AI509029 Six3 1424759_at 5.006 BC017528 Arrdc4 1450930_at 5.167 AK002992 Hpca 1417393_a_at 4.997 NM_026125 C1qdc2 1433987_at 5.163 AV153557 Hpcal4 1435588_at 4.996 BQ031098 1419742_at 5.16 NM_026091 1700037H04Rik 1450486_a_at 4.991 X91813 Oprl1 1440371_at 5.151 BQ176223 1455108_at 4.989 BM119888 Eif4e2 1434825_at 5.151 BQ175287 LOC381742 1459920_at 4.988 AA163908 4933415E08Rik 1423420_at 5.148 AK018378 Adrb1 1417216_at 4.986 NM_138606 Pim2; 1434415_at 5.141 BB797871 LOC229005 1417716_at 5.139 U82470 Got2 1457696_at 4.982 AI835553 Rilp 1449188_at 5.139 NM_021565 Midn 1452938_at 4.979 BQ174247 C030032C09Rik 1417032_at 5.138 AF296657 Ube2g2 1433450_at 4.978 BB177836 Cdk5r1 1441815_at 5.136 AI851453 AI851453 1418172_at 4.974 AF117613 Hebp1 1435767_at 5.132 BE951842 Scn3b 1422552_at 4.965 NM_023396 Rprm 1426430_at 5.131 AV264681 Jag2 1422255_at 4.964 NM_021275 Kcna4 1438913_x_at 5.127 AW322533 Hdgfrp2 1423442_a_at 4.962 BE854125 Fbxw2 1449007_at 5.127 NM_009770 Btg3 1460285_at 4.947 NM_133721 Itga9 1434805_at 5.127 BG063049 Mllt1 1452911_at 4.943 AK017680 Spred1 1423049_a_at 5.126 AK002271 Tpm1 1452851_at 4.937 AK019578 Tnrc4 1423352_at 5.123 BE693025 Crispld1 1422609_at 4.936 BE648432 Arpp19 1424475_at 5.121 BI157430 Camkk2 1429174_at 4.936 AK014304 Wdr34 1452783_at 5.118 AK005500 Fndc3b 1448922_at 4.934 NM_024438 Dusp19 1425363_at 5.113 BC022180 Galgt1 1425711_a_at 4.932 M94335 Akt1 1455313_at 5.104 BB336053 Ablim2 1454844_at 4.929 AW049955 Gpr24 1455703_at 5.102 BG074736 Akt2 1416155_at 4.928 NM_008253 Hmgb3 1434832_at 5.099 BB364488 Foxo3a 1423311_s_at 4.927 BQ177165 Tpbg 1439888_at 5.096 BB271581 1418497_at 4.922 AF020737 Fgf13 1433747_at 5.093 BQ176475 Lnpep 1418463_at 4.915 NM_008841 Pik3r2 1435206_at 5.092 BB767169 Slc24a4 1416448_at 4.914 NM_025922 Itpa 1428173_at 5.088 AK005562 Eml2 1428357_at 4.912 AK011462 2610019F03Rik 1416586_at 5.084 NM_008616 Zfp239 1450269_a_at 4.907 NM_008826 Pfkl 1442370_at 5.082 BB267006 1424199_at 4.905 AW537349 Seh1l 1417311_at 5.075 NM_024223 Crip2 1433833_at 4.902 BG064539 Fndc3b 1440166_x_at 5.066 BB378627 Htr1d 1426383_at 4.898 BF303057 Ciy2 1416402_at 5.066 AV382118 Abcb10 1417626_at 4.898 NM_031401 Usmg4 1422807_at 5.065 NM_007480 Arf5 1415998_at 4.891 NM_011694 Vdac1 1455771_at 5.062 BB467799 Bzrap1 1424208_at 4.891 BC011193 Ptger4 1418417_at 5.058 NM_010827 Msc 1454086_a_at 4.889 AK013416 Lmo2 1448411_at 5.055 NM_011716 Wfs1 1424503_at 4.886 BC006596 Rab22a 1435598_at 5.043 BE996371 Shc2 1456766_at 4.883 BB036966 C330001K17Rik 1423415_at 4.883 BB110067 Gpr83 1427912_at 4.699 AK003232 Cbr3 1434082_at 4.882 BM243464 Pctk2 1458351_s_at 4.697 BB428573 Klhl2 1433707_at 4.881 BB430205 Gabra4 1422762_at 4.697 AB008867 Kif17 1426710_at 4.878 BB396904 Calm3 1433962_at 4.693 BB131965 6720458F09Rik 1417897_at 4.878 NM_134155 Brms1 1458518_at 4.692 BM935843 Cpeb2 1437292_at 4.874 BB431052 A330019N05Rik 1426792_s_at 4.691 BC024790 Rusc2 1416605_at 4.874 BC024944 Nola2 1422718_at 4.688 NM_009682 Ap3s2 1425051_at 4.872 AK010892 Isoc1 1440258_at 4.685 BE951512 Kcnq2 1426539_at 4.872 AI117611 Usp11 1443225_at 4.684 BB396526 Acvr1c 1427006_at 4.87 BB339051 Rapgef1 1416409_at 4.68 AB034914 Acox1 1434563_at 4.87 BM207149 Rps6kc1 1416215_at 4.68 NM_016810 Gosr1 1452292_at 4.869 AV271093 Ap2b1 1417003_at 4.679 BC021536 0610012G03Rik 1421975_a_at 4.868 AF189769 Add2 1434904_at 4.678 AW321867 Hivep2 1455024_at 4.868 AV269836 Tlk1 1428434_at 4.677 AK012833 Zcchc12 1423519_at 4.855 BE457744 2210412D01Rik 1436225_at 4.676 BB082386 Trpm2 1442180_at 4.855 BB382040 Dleu7 1439867_at 4.676 BB079377 Ubox5 1435974_at 4.855 BB271482 Arhgef9 1429229_s_at 4.674 BE980134 4930534B04Rik 1428839_at 4.854 AK005105 Wdr53 1421046_a_at 4.673 NM_130881 Pabpc4; 1431751_a_at 4.85 AK012553 Mpped2 LOC432881 1426069_s_at 4.849 BC016100 Slc7a4 1453323_at 4.664 AK013806 2900079G21Rik 1448285_at 4.848 NM_009062 Rgs4 1429392_at 4.656 BI558553 Wdr40a 1452092_at 4.848 AK019474 4631426J05Rik 1428718_at 4.656 AW490544 Scrn1 1456144_at 4.842 AW537064 Nav3 1424225_at 4.649 AF398969 Asb8 1454797_at 4.838 AW556098 Tmem55b 1435942_at 4.647 BE993301 Kcnq2 1426465_at 4.838 BG066219 Dlgap4 1447711_x_at 4.646 BB265147 4933412E12Rik 1427954_at 4.837 BC028785 BC048403 1439497_at 4.637 BG065013 4933415E08Rik 1451914_a_at 4.834 AF100423 Add2 1453734_at 4.633 BF165715 Atrx 1442562_at 4.83 BB346556 D630040G17Rik 1419056_at 4.632 AF038538 Rtn2 1450710_at 4.825 NM_021878 Jarid2 1447124_at 4.631 BE634581 1449147_at 4.821 NM_023850 Chst1 1447861_x_at 4.629 AV329643 Mrg1 1435285_at 4.816 BB731805 Mpped2 1425844_a_at 4.622 AF034568 Rngtt 1425031_at 4.81 AB077383 Fcmd 1449063_at 4.614 BC009024 Sec22l1 1433714_at 4.808 AV338618 Sult4a1 1451722_s_at 4.611 BF160651 Smyd5 1421606_a_at 4.794 NM_013873 Sult4a1 1417349_at 4.611 NM_019788 Pldn 1450008_a_at 4.783 NM_007614 Ctnnb1 1429367_at 4.611 BB826276 2510001I10Rik 1421107_at 4.778 NM_021420 Stk4 1420901_a_at 4.609 NM_010438 Hk1 1437579_at 4.773 C77054 Nek2 1437017_at 4.606 BB046727 AI480653 1434058_at 4.772 BB667459 Mtmrl2 1420985_at 4.606 BG694892 Ash1l 1432295_a_at 4.77 AK009634 2310035K24Rik 1428878_a_at 4.602 AI595920 Pitpnc1 1419737_a_at 4.769 NM_010699 Ldh1 1436387_at 4.601 BB398124 C330006P03Rik 1423200_at 4.765 U22016 Ncor1 1427232_at 4.601 AV291373 Sdccag33 1449770_x_at 4.753 N28171 D16Bwg1494e 1426978_at 4.6 AW682368 Klhl2 1416683_at 4.75 NM_138749 Plxnb2 1416550_at 4.6 BB320416 Slc35b4 1425718_a_at 4.747 BC004092 Ivns1abp 1428903_at 4.599 AK014134 3110037I16Rik 1422590_at 4.745 NM_007668 Cdk5 1440376_at 4.597 AI747062 Fbxo41 1438196_at 4.739 BB727537 Gpd1l 1434228_at 4.594 AV255921 Ppm2c 1417794_at 4.738 NM_019831 Zfp261 1460030_at 4.594 BB355811 Hecw1 1434693_at 4.737 BE952823 1450484_a_at 4.594 AK004595 Tyki 1442099_at 4.733 BM227490 Usp31 1455140_at 4.593 AV376568 A330068P14Rik 1442220_at 4.733 BB313387 Ube3a 1451998_at 4.592 BC024597 4930485D02Rik 1435547_at 4.728 BQ175722 Mkl2 1460116_s_at 4.59 AI450584 Spred1 1426051_a_at 4.725 BC006628 Cenpb 1427286_at 4.587 BB130195 D11Bwg0517e 1434476_at 4.713 BM119896 Mect1 1437861_s_at 4.584 BB335101 Prkce 1440108_at 4.711 BM964154 Foxp2 1445605_s_at 4.581 AI835491 4921533L14Rik 1423330_at 4.708 AK006149 Ensa 1415803_at 4.578 AF010586 Cx3cl1 1429443_at 4.706 AK014396 Cpne4 1449511_a_at 4.577 NM_133772 Ssbp4 1419420_at 4.577 NM_012028 St6galnac5 1416853_at 4.47 BC017126 Ncdn 1425656_a_at 4.575 AF390178 Baiap2 1426644_at 4.464 BC002196 Tbc1d20 1435222_at 4.575 BM220880 Foxp1 1434339_at 4.461 AW548221 Fnbp1l 1428864_at 4.575 AK017419 5530400B01Rik 1428327_at 4.46 BB010301 2310001H13Rik 1423831_at 4.575 BB756794 Prkag2 1451138_x_at 4.459 BC005748 2700087H15Rik 1439148_a_at 4.574 BE914497 Pfkl 1449863_a_at 4.459 NM_010056 Dlx5 1428540_at 4.566 BE944524 3321401G04Rik 1417178_at 4.458 NM_016867 Gipc2 1426742_at 4.562 AK019459 Atp5f1 1423269_a_at 4.456 BB663717 Nedd4l 1455026_at 4.56 BE947961 Sbno1 1416292_at 4.454 NM_007452 Prdx3 1452234_s_at 4.555 BF457957 D16Bwg1494e 1435698_at 4.452 BQ176694 4921505C17Rik 1425760_a_at 4.554 AF006467 Pitpnm1 1419838_s_at 4.45 AI385771 Plk4 1434682_at 4.554 AV347367 6430601A21Rik 1452063_at 4.448 AK010736 2410081M15Rik 1428095_a_at 4.552 AK004920 Tmem24 1423038_at 4.446 BQ174465 Stx6 1447696_x_at 4.552 BB091427 Adcy5 1424645_at 4.446 BB795102 Tnrc6c 1415710_at 4.55 BM123013 BC038311 1434102_at 4.441 BI687652 Nfib 1455482_at 4.54 BQ175609 Ap2a2 1422431_at 4.439 NM_053201 Magee1 1435425_at 4.539 AV212090 4933439C20Rik; 1459994_x_at 4.435 AV027486 Trfr2 Pisd 1418467_at 4.433 NM_025891 Smarcd3 1438841_s_at 4.539 AV002218 Arg2 1428941_at 4.429 AK017929 Zfp198 1423785_at 4.538 BE995700 Egln1 1439422_a_at 4.429 AV048291 C1qdc2 1453000_at 4.538 AK005444 Camsap1l1 1424480_s_at 4.428 BC026151 Akt2 1429623_at 4.534 AV261187 Zfp644 1419358_at 4.425 NM_030889 Sorcs2 1418110_a_at 4.533 U39203 Inpp5d 1451746_a_at 4.424 AK016474 Atg12 1460223_a_at 4.531 NM_013514 Epb4.9 1454741_s_at 4.421 BG064061 AW547186 1418168_at 4.53 BB223737 Zcchc14 1455247_at 4.419 BB767281 Amotl1 1437366_at 4.525 BB332542 AI608492 1430614_at 4.412 BG962576 4632415K11Rik 1455074_at 4.521 AV307860 1435078_at 4.411 BQ173895 3526402J09Rik 1453077_a_at 4.521 AK016168 Snapc3 1452269_at 4.411 AF026489 Spnb3 1434032_at 4.519 BQ175381 1427930_at 4.405 BG063905 Pdxk 1417709_at 4.519 NM_010010 Cyp46a1 1420810_at 4.403 NM_019769 1500003O03Rik 1428924_at 4.514 AU016306 Mocs3 1417829_a_at 4.401 NM_134050 Rab15 1453428_at 4.514 AK012380 2700045P11Rik 1416287_at 4.401 NM_009062 Rgs4 1423195_at 4.512 BM208582 Hiat1 1422473_at 4.398 BM246564 Pde4b 1450918_s_at 4.503 BG868120 Src 1455961_at 4.397 AV174022 Mme 1457695_at 4.502 C76297 Ap1gbp1 1436513_at 4.396 BM208226 3526402J09Rik 1451028_at 4.501 AA796998 Baiap2 1431711_a_at 4.393 AK018497 9030409G11Rik 1442558_at 4.501 BB180191 1425452_s_at 4.388 BC002154 Ptprj; AW125753 1438067_at 4.497 BB526552 Nf1 1432184_a_at 4.387 AK011884 2610204M08Rik 1459107_at 4.496 BG069378 Kcnh3 1433699_at 4.387 BM241351 Tnfaip3 1438039_at 4.493 BE630599 Hectd1 1417225_at 4.386 NM_022992 Arl6ip5 1433526_at 4.493 BM942651 Klhl8 1436013_at 4.384 A1852434 C230098I05Rik 1460191_at 4.49 NM_019661 0610042I15Rik 1455156_at 4.382 BG519214 Strn 1447771_at 4.49 AW050081 1448378_at 4.382 NM_007984 Fscn1 1442883_s_at 4.488 BI413749 D10Bwg1364e 1437390_x_at 4.378 AV339210 Stx1a 1455023_at 4.487 BB171181 N28178 1455883_a_at 4.375 BB269910 Lrrtm1 1428550_at 4.486 AK007503 1810015A11Rik 1431875_a_at 4.374 AK017841 E2f1 1426643_at 4.485 AK012072 Elp3 1455888_at 4.369 BB125202 Lrrn6c 1453317_a_at 4.484 AK014353 Khdrbs3 1457046_s_at 4.369 BB545241 C77370 1460436_at 4.479 BI652065 Ndst1 1454977_at 4.365 AW551191 AU020772 1429124_s_at 4.477 BQ178367 Rexo1 1432271_a_at 4.364 AK012764 Dcun1d5 1418235_at 4.477 AV168389 Atg5 1423606_at 4.36 BI110565 Postn 1427058_at 4.475 AK010644 Eif4a1 1455548_at 4.357 BB314358 Dlgap4 1453412_a_at 4.475 BI652727 Sec14l1 1436081_a_at 4.354 BE632205 Zfp414 1425104_at 4.473 BC022615 Kctd1 1425710_a_at 4.352 AB019479 Homer1 1430612_at 4.471 BB533148 1810033B17Rik 1416528_at 4.349 NM_080559 Sh3bgrl3 1426972_at 4.471 AK009425 Sec24d 1450038_s_at 4.346 AW107303 Usp9x 1453021_at 4.345 BM899291 Stxbp5 1432091_a_at 4.307 AK007455 Rutbc3 1454675_at 4.344 BI076689 Thra 1448196_at 4.305 NM_134017 Mat2b 1440455_at 4.341 AI848599 AI848599 1431811_a_at 4.303 AK020018 Fbxo34 1433499_at 4.333 BF585125 2010005J08Rik 1458133_at 4.302 BB360008 1432478_a_at 4.333 AK015966 Ibrdc3 1457080_at 4.302 BB046159 1429678_at 4.325 AK017758 5730508B09Rik 1449932_at 4.3 NM_027874 Csnk1d 1460563_at 4.317 BE949414 1433937_at 4.298 BB814564 Trp53bp2 1424418_at 4.316 BC010801 BC010801 1455772_at 4.296 BB428874 Pgr 1452925_a_at 4.314 AK009364 5-Mar 1453069_at 4.292 BF018155 Pik3cb 1428354_at 4.312 BM206907 Foxk2 1416983_s_at 4.289 AI462296 Foxo1 1417481_at 4.311 NM_016894 Ramp1 1432813_at 4.289 AK013749 2900064F13Rik 1422677_at 4.311 AK002443 Dgat2 1436786_at 4.289 AV024133 1110069O07Rik 1415975_at 4.31 AU080787 Carhsp1 1458967_at 4.289 BM939341 1456601_x_at 4.309 AV002675 Fxyd2 1425327_at 4.288 AU067643 BC008163 1441917_s_at 4.307 BB468188 Tmem40 ProbeSymbol = Affymetrix probe identification corresponding to gene symbol; FEN = fold enrichment when compared to reference sample; Genbank = Genbank identification; GeneSymbol = official gene symbol.

TABLE 18 Top 1,000 striatopallidal (D2) cell translated mRNAs enriched when compared to a reference sample ProbeSymbol FEN Genbank GeneSymbol ProbeSymbol FEN Genbank GeneSymbol 1435211_at 183.2 BB554351 Ttc12 1449425_at 16.64 BC026373 Wnt2 1426814_at 104.8 BM248309 AU024582 1422079_at 16.52 NM_008856 Prkch 1452217_at 88.94 BE570050 Ahnak 1437698_at 15.91 AV370579 Myo5b 1440148_at 80.24 AI852874 Gpr6 1424507_at 15.72 BC011277 Rin1 1418950_at 72.42 NM_010077 Drd2 1454581_at 15.58 AK019908 5330425B07Rik 1419231_s_at 65.33 NM_010661 Krt1-12 1433566_at 15.41 BB381618 Rasl10b 1418357_at 52.9 NM_008241 Foxg1 1441368_at 15.29 BB102769 1426815_s_at 44.02 BM248309 AU024582 1452966_at 15.02 AK020296 9130430L19Rik 1457132_at 43.55 BF456117 1450072_at 14.94 BG694892 Ash1l 1443287_at 42.58 BB555669 Gm1337 1455190_at 14.83 BM114283 Gng7 1438022_at 40.99 BQ266518 Rab11fip3 1419230_at 14.66 NM_010661 Krt1-12 1435564_at 40.44 BB547893 C230078M08Rik 1449420_at 14.07 NM_008800 Pde1b 1436268_at 37.26 AI841578 Ddn 1427807_at 14.06 BC017159 4930448N21Rik 1458499_at 36.61 AW123977 Pde10a 1452879_at 13.95 AI848603 Synpo2 1421365_at 32.61 NM_008046 Fst 1452176_at 13.95 BB292874 Nup153 1454867_at 32.18 BB234631 Mn1 1425870_a_at 13.88 AF439339 Kcnip2 1420437_at 31.55 NM_008324 Indo 1434653_at 13.48 AV026976 Ptk2b 1460710_at 28.67 BB748999 1418619_at 13.42 NM_008319 Icam5 1423171_at 27.79 BE947345 Gpr88 1458342_at 13.38 BB313069 1423117_at 24.88 BB837171 Pum1 1436566_at 13.32 AV364488 Rab40b 1434458_at 23.89 BB444134 Fst 1435227_at 12.96 BM117007 1450142_a_at 21.46 NM_130886 Card14 1451280_at 12.87 BB159263 MGI: 107562 1434917_at 20.15 BQ173923 Cobl 1425503_at 12.63 AB037596 Gcnt2 1450339_a_at 19.88 NM_021399 Bcl11b 1454043_a_at 12.44 AK015412 Kcnab1 1435366_at 19.68 BB486367 D430042O09Rik 1436650_at 12.37 AV241894 Filip1 1427017_at 19.47 BB104560 Satb2 1449241_at 12.35 NM_053105 Klhl1 1460325_at 19.42 BB837171 Pum1 1456054_a_at 12.32 BB314559 Pum1 1439854_at 18.58 BQ175572 AI838259 1423530_at 12.24 BB320288 Stk32c 1423802_at 18.24 BC017634 Camky 1452202_at 12.21 BG069616 Pde2a 1450071_at 17.88 BG694892 Ash1l 1444734_at 12.08 BB183877 A330001L22Rik 1418782_at 17.83 NM_009107 Rxrg 1417356_at 12.04 AB003040 Peg3 1436275_at 17.8 AW490636 Kcnip2 1433627_at 11.96 AW546839 Sec23ip 1457143_at 17.66 BB322292 Gli3 1418847_at 11.93 NM_009705 Arg2 1456283_at 11.85 AV346211 Neto1 1452298_a_at 8.862 AW546331 Myo5b 1450026_a_at 11.85 AV306734 B3gnt1 1451917_a_at 8.818 AF155820 Dcamkl1 1435615_at 11.83 BB277790 Zfp365 1424037_at 8.796 BC027291 Itpka 1455683_a_at 11.73 BB451404 Tbc1d8 1422621_at 8.772 BM507707 Ranbp2 1436329_at 11.4 AV346607 Egr3 1443334_at 8.709 BB487579 D430042O09Rik 1428338_at 11.3 AK019166 2610039E05Rik 1422153_a_at 8.653 NM_026853 Asb11 1424248_at 11.23 BB159263 MGI: 107562 1415904_at 8.636 BC003305 Lpl 1434521_at 11.2 BB148972 Rfxdc2 1439552_at 8.633 BE985618 Trio 1427358_a_at 11.19 BC026671 Dapk1 1437460_x_at 8.63 BB264363 Rin1 1435083_at 11.09 BI155559 Ctxn1 1427683_at 8.629 X06746 Egr2 1451331_at 11.09 BC026568 Ppp1r1b 1460136_at 8.61 AI462839 1417090_at 10.95 NM_009037 Rcn1 1448990_a_at 8.524 AI255256 Myo1b 1457311_at 10.92 AW490258 Camk2a 1427931_s_at 8.518 BG063905 Pdxk 1460038_at 10.84 BG065255 Pou3f1 1429089_s_at 8.504 BG063749 2900026A02Rik 1451236_at 10.81 BC026463 Rerg 1427673_a_at 8.502 Z93948 Sema3e 1448807_at 10.78 NM_133849 Hrh3 1419224_at 8.47 NM_033567 Cecr6 1417804_at 10.75 NM_011242 LOC381240 1422068_at 8.454 NM_011141 Pou3f1 1452476_at 10.64 W41214 Cacnb2 1429285_at 8.419 AK009343 Serpina9 1438355_at 10.63 AI414870 1460244_at 8.419 NM_133995 Upb1 1418015_at 10.61 BI689507 Pum2 1434295_at 8.405 BE691356 Rasgrp1 1458406_at 10.6 BG144063 1438985_x_at 8.393 BB158937 Otud5 1427005_at 10.53 BM234765 Plk2 1438889_at 8.391 BB183456 1426980_s_at 10.29 BC006054 E130012A19Rik 1434248_at 8.328 BM243756 Prkch 1450213_at 10.17 NM_013875 Pde7b 1456401_at 8.318 BB078175 Cacnb2 1442166_at 10.03 BB273427 Cpne5 1419696_at 8.287 NM_013488 Cd4 1435749_at 9.907 AW911807 Gda 1441733_s_at 8.245 C88147 Nup153 1423561_at 9.752 AI838010 Nell2 1421141_a_at 8.234 BG962849 Foxp1 1456640_at 9.723 AW910872 Sh3rf2 1430127_a_at 8.229 AK007904 Ccnd2 1455754_at 9.708 BQ043839 Lmo3 1454721_at 8.185 AV257687 1110018G07Rik 1428150_at 9.671 BB203098 Coro7 1454768_at 8.147 AV337635 Kcnf1 1423186_at 9.628 BM228957 Tiam2 1421140_a_at 8.143 BG962849 Foxp1 1444681_at 9.613 BB749686 D14Ertd171e 1437586_at 8.123 BB756908 Cnot4 1454906_at 9.596 BB266455 Rarb 1455629_at 8.11 BE957273 Drd1a 1425833_a_at 9.537 AF326551 Hpca 1460343_at 8.092 AF401228 Neurl 1418169_at 9.532 BB223737 Zcchc14 1418881_at 8.09 NM_054095 Efcbp2 1418714_at 9.511 NM_008748 Dusp8 1435741_at 8.074 BB312125 Pde8b 1425756_at 9.479 AF425643 Rab40b 1455701_at 8.033 B1965517 Snx26 1433701_at 9.466 BB371430 Mpped1 1434008_at 8.029 BE993937 Scn4b 1448327_at 9.366 NM_033268 Actn2 1435748_at 8.028 AW911807 Gda 1419517_at 9.345 NM_028408 Cnih3 1460555_at 8.026 BM242294 6330500D04Rik 1441000_at 9.343 BB076832 1435221_at 7.968 BM220880 Foxp1 1451604_a_at 9.334 BC014291 Acyrl1 1436532_at 7.932 BB326709 Dcamkl3 1422710_a_at 9.333 NM_021415 Cacna1h 1450061_at 7.921 BM120053 Enc1 1440204_at 9.247 AW494150 3110039M20Rik 1454845_x_at 7.898 AW049955 Gpr24 1417680_at 9.208 NM_008419 Kcna5 1423544_at 7.878 BB188812 Ptpn5 1438799_at 9.154 AV338180 Dlx6os1 1457341_at 7.864 BE991676 1433815_at 9.13 AV290082 MGI: 1923321 1427343_at 7.862 BC026377 Rasd2 1418373_at 9.111 NM_018870 Pgam2 1416996_at 7.811 BC005421 Tbc1d8 1458407_s_at 9.046 BG144063 1417355_at 7.81 AB003040 Peg3 1434115_at 9.043 BQ176681 1452135_at 7.783 AV001252 Gpx6 1449158_at 8.965 NM_010607 Kcnk2 1451517_at 7.723 AF420001 Rhobtb2 1435334_at 8.945 BG866904 Ttc7 1428547_at 7.718 AV273591 Nt5e 1438665_at 8.935 BF456582 Smpd3 1436738_at 7.707 AV094878 AI449441 1437125_at 8.93 BB476448 Camk2a 1418691_at 7.696 NM_011268 Rgs9 1460567_at 8.928 BB148972 Rfxdc2 1455361_at 7.674 AW493391 Dgkb 1435780_at 8.914 BG966595 Psd 1452507_at 7.668 AF022078 Dlx6 1422706_at 7.638 AV370981 Tmepai 1452452_at 6.863 M73818 1456858_at 7.624 BB075339 Gpr149 1420580_at 6.861 NM_026249 4930429B21Rik 1427519_at 7.603 BG311385 Adora2a 1416350_at 6.855 NM_078477 Klf16 1450748_at 7.6 NM_021491 Smpd3 1424409_at 6.851 BC019534 Cldn23 1420146_at 7.544 AI429207 Tiam2 1455272_at 6.83 BB429139 Grm5 1421739_a_at 7.531 NM_010768 Matk 1416456_a_at 6.824 BC011134 Chia 1438841_s_at 7.492 AV002218 Arg2 1457052_at 6.783 BG064867 AW536275 1427015_at 7.466 BI732921 LOC380969 1428951_at 6.765 AK017551 Nol8 1418908_at 7.398 NM_013626 Pam 1431936_a_at 6.749 AK009828 Neu2 1419221_a_at 7.395 NM_016758 Rgs14 1439767_at 6.715 BB130159 Dlgap2 1459299_at 7.39 AV381193 A430065P19Rik 1419216_at 6.709 NM_009734 Azi1 1425479_at 7.377 BF160651 Smyd5 1455564_at 6.703 BQ176236 Bcr 1422165_at 7.374 X66603 Pou3f4 1429617_at 6.702 BM119209 Cyld 1416804_at 7.373 NM_053252 LOC114601 1447178_at 6.679 BB173057 1454762_at 7.368 BM932447 Xkrx 1444441_at 6.676 AW490909 1427344_s_at 7.364 BC026377 Rasd2 1421101_a_at 6.671 NM_010698 Ldb2 1420852_a_at 7.355 AV306734 B3gnt1 1427975_at 6.648 AK008807 2210403B10Rik 1433767_at 7.344 AV257687 1110018G07Rik 1421000_at 6.647 AI448404 Cnot4 1452368_at 7.313 AI853148 Bcr 1420545_a_at 6.634 NM_029716 Chn1 1416691_at 7.294 NM_019581 Gtpbp2 1445539_at 6.63 BE687857 Pde7b 1426915_at 7.292 BC021490 Dapk1 1419542_at 6.615 NM_010021 Dazl 1454752_at 7.276 AV307961 AI606861 1439557_s_at 6.585 BB097063 Ldb2 1417279_at 7.259 NM_010585 Itpr1 1436998_at 6.578 BB428991 Ankrd43 1456336_at 7.245 BB182912 A330102K23Rik 1424796_at 6.575 BC024705 1700054N08Rik 1419389_at 7.231 BQ180352 Pde10a 1456468_x_at 6.555 BB189996 1110012N22Rik 1455447_at 7.218 BM116882 D430019H16Rik 1455301_at 6.535 BG064092 BQ952480 1450690_at 7.212 BM507707 Ranbp2 1422718_at 6.531 NM_009682 Ap3s2 1422705_at 7.205 AV370981 Tmepai 1456051_at 6.527 BB282271 Drd1a 1455200_at 7.202 BB818370 Pak6 1456818_at 6.464 BB279083 Stk32a 1422678_at 7.198 AK002443 Dgat2 1427523_at 6.461 AI509029 Six3 1436066_at 7.162 BI965477 Kalrn 1423240_at 6.423 BG868120 Src 1447359_at 7.13 AI326876 LOC381955 1449634_a_at 6.416 AW046296 C030032C09Rik 1423231_at 7.123 AK002933 Nrgn 1426901_s_at 6.376 BB657856 Camta2 1436408_at 7.113 BE946298 1440534_at 6.373 BB177862 1420978_at 7.112 NM_010938 Nrf1 1438417_at 6.362 BB809239 Pwwp2 1436216_s_at 7.097 BM234799 2610204M08Rik 1426572_at 6.359 BM235734 Me2 1451726_at 7.091 BC020019 Mtmr6 1452380_at 6.348 BB075797 Epha7 1442434_at 7.087 BM195829 D8Ertd82e 1422208_a_at 6.347 BC016135 Gnb5 1453782_at 7.086 BI737125 3021401C12Rik 1435435_at 6.333 BB357580 Cttnbp2 1448364_at 7.072 U95826 Ccng2 1454877_at 6.319 BQ174721 Sertad4 1435296_at 7.051 AV349563 Adra2c 1419754_at 6.298 NM_010864 Myo5a 1438210_at 7.049 BB126999 Gpr149 1420666_at 6.296 BM117900 Doc2b 1416776_at 7.04 NM_016669 Crym 1424028_at 6.292 BCO23107 5830457O10Rik 1432073_at 7.007 AK007198 1700113I22Rik 1417440_at 6.266 NM_033566 Arid1a 1432972_at 7.004 AK014916 4921518B13Rik 1453886_a_at 6.265 AK017037 Slc25a26 1426518_at 6.977 AV333951 Tubgcp5 1456946_at 6.262 BE949337 4831416G18Rik 1427045_at 6.977 AI849322 Synpo 1420377_at 6.23 BG071333 St8sia2 1429156_at 6.97 BF453953 2610036L11Rik 1443365_at 6.216 BB431008 Htr4 1417129_a_at 6.954 U68384 Mrg1 1416437_a_at 6.213 AF262046 Mapk8ip3 1429422_at 6.945 AK016788 4933412E12Rik 1427682_a_at 6.21 X06746 Egr2 1425130_a_at 6.941 U28216 Ptpn5 1448978_at 6.205 NM_019867 Ngef 1421353_at 6.94 NM_013875 Pde7b 1454656_at 6.19 AV271736 Spata13 1439569_at 6.925 AV291031 Gpr83 1428535_at 6.16 AK004276 9430020K01Rik 1430675_at 6.915 BM932606 2900055J20Rik 1455178_at 6.142 BB496468 Rutbc1 1452618_at 6.887 BM230774 Cdk5rap2 1416562_at 6.137 AF326547 Gad1 1439566_at 6.882 BB245373 C030038J10Rik 1448709_at 6.131 NM_033566 Arid1a 1457979_at 6.128 BM938335 1453836_a_at 5.523 AK006949 Mgll 1435666_at 6.098 AW553439 1416050_a_at 5.505 NM_016741 Scarb1 1433583_at 6.093 AV327248 Zfp365 1420985_at 5.46 BG694892 Ash1l 1422018_at 6.075 NM_010437 Hivep2 1450655_at 5.455 AA214868 Herc1 1455056_at 6.068 BM231903 Lmo7 1450042_at 5.44 BB322201 Arx 1436092_at 6.045 BB336256 1417626_at 5.435 NM_031401 Usmg4 1455296_at 6.031 BE952286 Adcy5 1421116_a_at 5.427 NM_024226 Rtn4 1420534_at 6.016 AK004815 Gucy1a3 1438784_at 5.42 BB329234 1421142_s_at 6.012 BG962849 Foxp1 1434775_at 5.419 AW543460 Pard3 1427450_x_at 6.007 BI080370 Myo1b 1419390_at 5.411 BQ180352 Pde10a 1434429_at 6.002 AV348245 Syt16 1418017_at 5.398 BI689507 Pum2 1423420_at 5.998 AK018378 Adrb1 1418535_at 5.387 NM_016846 Rgl1 1416627_at 5.974 NM_016907 Spint1 1438068_at 5.378 BB251859 1435808_at 5.95 BB174377 A230051G13Rik 1435825_at 5.364 BG969012 Acvrl1 1440711_at 5.93 BB426320 C630001G18Rik 1431056_a_at 5.359 AK017272 Lpl 1435763_at 5.899 BI218684 Tbc1d16 1417612_at 5.347 BF147705 Ier5 1417114_at 5.894 AF163665 Gdl 1420871_at 5.339 BF472806 Gucy1b3 1453129_a_at 5.892 AK004813 Rgs12 1423532_at 5.337 AI850285 Rnf44 1417709_at 5.881 NM_010010 Cyp46a1 1438664_at 5.337 BB216074 Prkar2b 1453264_at 5.87 AK007346 Marveld3 1417812_a_at 5.325 NM_008484 Lamb3 1455703_at 5.867 BG074736 Akt2 1447861_x_at 5.322 AV329643 Mrg1 1427912_at 5.858 AK003232 Cbr3 1419033_at 5.32 AW556821 2610018G03Rik 1419137_at 5.825 NM_021423 Shank3 1455220_at 5.317 BB353860 Frat2 1455620_at 5.814 BQ177219 1435767_at 5.314 BE951842 Scn3b 1429111_at 5.808 AV270892 Tln2 1435285_at 5.312 BB731805 Mpped2 1433988_s_at 5.808 BG075755 C230098O21Rik 1439611_at 5.305 BE947522 Chrm1 1435210_s_at 5.785 BQ174936 Snph 1448063_at 5.302 AW226526 Iqsec2 1434090_at 5.771 BB037094 4930429A22Rik 1427426_at 5.295 AV362204 Kcnq5 1450143_at 5.766 BB354696 Rasgrp1 1421028_a_at 5.291 AI595932 Mef2c 1435265_at 5.763 BF466929 1421346_a_at 5.268 NM_009320 Slc6a6 1441963_at 5.744 BB427703 MGI: 2656976 1430013_at 5.263 BE993118 2210008F06Rik 1448023_at 5.741 BB662566 Kalrn 1452964_at 5.26 AK016577 4932702F08Rik 1447771_at 5.706 AW050081 1422787_at 5.259 NM_019873 Fkbpl 1416286_at 5.696 NM_009062 Rgs4 1450227_at 5.254 BM199504 Ankrd6 1460206_at 5.688 NM_019518 Grasp 1426450_at 5.25 BM207017 Plcl2 1423376_a_at 5.685 AV341904 Dok4 1441468_at 5.242 BB326028 1435558_at 5.683 BB351248 Bai2 1436733_at 5.239 BB523550 E130309F12Rik 1455961_at 5.675 AV174022 Mme 1429316_at 5.234 AK018120 Rasgef1a 1447017_at 5.661 BE956696 1440166_x_at 5.232 BB378627 Htr1d 1460285_at 5.647 NM_133721 Itga9 1455225_at 5.217 AV237615 Syne1 1432184_a_at 5.611 AK011884 2610204M08Rik 1435043_at 5.214 BB794831 Plcb1 1431751_a_at 5.605 AK012553 Mpped2 1417522_at 5.211 AF441120 Fbxo32 1422902_s_at 5.594 NM_023799 Mgea5 1448468_a_at 5.207 AF033003 Kcnab1 1431394_a_at 5.59 AK014938 Lrrk2 1452295_at 5.207 AV291712 Tmepai 1434359_at 5.589 AV329070 6330500D04Rik 1426037_a_at 5.192 U94828 Rgs16 1445691_at 5.588 AW121426 Chn1 1434745_at 5.186 BQ175880 Ccnd2 1435071_at 5.583 AV327165 Zfyve1 1447728_x_at 5.183 BB718260 Hspa9a 1425510_at 5.578 BM213279 Mark1 1426024_a_at 5.177 AF187147 Dbn1 1427038_at 5.577 M13227 Penk1 1448989_a_at 5.176 AI255256 Myo1b 1417462_at 5.576 NM_007598 Cap1 1416398_at 5.172 BB474887 Mesdc1 1433937_at 5.559 BB814564 Trp53bp2 1429637_at 5.163 AK008987 2210419I08Rik 1415743_at 5.537 NM_010412 Hdac5 1450930_at 5.161 AK002992 Hpca 1452861_at 5.534 AK008485 2010300C02Rik 1442558_at 5.16 BB180191 1453273_at 5.533 AV142265 Kcnv1 1433962_at 5.153 BB131965 6720458F09Rik 1419358_at 5.53 NM_030889 Sorcs2 1441894_s_at 5.148 BB071890 Grasp 1460352_s_at 5.526 BC017537 Pik3r4 1447707_s_at 5.127 BB367174 Pde2a 1433451_at 5.109 BB177836 Cdk5r1 1438224_at 4.663 BE572265 Zswim5 1422609_at 5.084 BE648432 Arpp19 1448598_at 4.662 NM_011846 Mmp17 1420530_at 5.083 AW553317 Neud4 1415834_at 4.658 NM_026268 Dusp6 1432646_a_at 5.076 BE859789 2900097C17Rik 1435218_at 4.656 BI134758 Rasgef1a 1423285_at 5.065 BB731671 Coch 1416683_at 4.647 NM_138749 Plxnb2 1435605_at 5.039 BB125424 Actr3b 1416983_s_at 4.643 AI462296 Foxo1 1451483_s_at 5.039 BC024705 1700054N08Rik 1437929_at 4.642 AV306847 Dact2 1428321_at 5.027 AK003593 Emil1 1452092_at 4.641 AK019474 4631426J05Rik 1448842_at 5.02 NM_033037 Cdo1 1433450_at 4.634 BB177836 Cdk5r1 1420496_at 4.998 NM_021489 F12 1435392_at 4.631 BB277182 Wdr17 1442113_at 4.991 BB183350 5330417C22Rik 1451475_at 4.628 BC019530 Plxnd1 1451078_at 4.988 BC012878 2510039O18Rik 1420981_a_at 4.628 NM_010723 Lmo4 1459679_s_at 4.975 AA406997 Myo1b 1418144_a_at 4.62 NM_008847 Pip5k1b 1425363_at 4.931 BC022180 Galgt1 1452917_at 4.614 AK011489 Rfc5 1434141_at 4.928 BG072799 Gucy1a3 1425466_at 4.611 AV317107 Senp2 1454832_at 4.917 BG228702 Phactr1 1421622_a_at 4.611 NM_019688 Rapgef4 1449863_a_at 4.911 NM_010056 Dlx5 1434153_at 4.603 BI408715 Shb 1434077_at 4.896 AV222037 Wdr37 1422977_at 4.594 NM_010327 Gp1bb 1423358_at 4.89 AK007407 1810009K13Rik 1455463_at 4.594 BB427286 Phyhip 1424379_at 4.877 BC019393 Car11 1422473_at 4.59 BM246564 Pde4b 1431320_a_at 4.872 AK002362 Myo5a 1426553_at 4.579 AK017830 Dnajc14 1435222_at 4.872 BM220880 Foxp1 1436002_at 4.579 BB484128 C230013L11Rik 1456786_at 4.87 BB097063 Ldb2 1435482_at 4.575 BB318003 Fibcd1 1454742_at 4.87 BB003229 Rasgef1b 1452851_at 4.573 AK019578 Tnrc4 1424201_a_at 4.864 AW537349 Seh1l 1426354_at 4.57 AK009033 Bap1 1423658_at 4.86 BC023131 Sppl3 1441764_at 4.562 BF730739 Prdm10 1441884_x_at 4.854 BB278992 Cx3cl1 1454759_at 4.56 AV230461 Git1 1422901_at 4.85 NM_023799 Mgea5 1434825_at 4.556 BQ175287 LOC381742 1440940_at 4.845 BE949510 Cacnb1 1437017_at 4.556 BB046727 AI480653 1448724_at 4.841 NM_009895 Cish 1435128_at 4.546 BB429313 Baiap2 1436645_a_at 4.84 BB066603 Cnot4 1419066_at 4.546 NM_030244 Ier5l 1429438_at 4.838 AV318805 Bcor 1448805_at 4.545 NM_009480 Usf1 1420872_at 4.79 BF472806 Gucy1b3 1437018_at 4.536 BG072348 Pnma2 1458133_at 4.784 BB360008 1439618_at 4.531 AI448308 Pde10a 1418172_at 4.778 AF117613 Hebp1 1437001_at 4.527 BQ173949 Gsk3b 1415803_at 4.775 AF010586 Cx3cl1 1421048_a_at 4.521 NM_023249 Ypel1 1423551_at 4.771 BB776961 Cdh13 1455881_at 4.52 BB078200 Ier5l 1419225_at 4.768 NM_009785 Cacna2d3 1449188_at 4.52 NM_021565 Midn 1423489_at 4.764 BC021914 Mmd 1428540_at 4.509 BE944524 3321401G04Rik 1435500_at 4.756 BB270778 Rab26 1426454_at 4.507 AK002516 Arhgdib 1449472_at 4.747 NM_008151 Gpr12 1429124_s_at 4.502 BQ178367 Rexo1 1457651_x_at 4.737 BB270375 Rem2 1425617_at 4.501 U91922 Dhx9 1424899_at 4.734 BC005737 Nmnat3 1417207_at 4.497 BF466091 Dvl2 1416982_at 4.725 AI462296 Foxo1 1422762_at 4.489 AB008867 Kif17 1423640_at 4.718 BC026512 Synpr 1451630_at 4.486 BC018513 Ttl 1441065_at 4.717 BB305753 1457632_s_at 4.485 BB207647 Mrg1 1427006_at 4.717 BB339051 Rapgef1 1443036_at 4.477 BG073535 C630007C17Rik 1455313_at 4.716 BB336053 Ablim2 1446840_at 4.475 BQ032894 1450448_at 4.714 BQ032752 Stc1 1449468_at 4.468 NM_012028 St6galnac5 1452974_at 4.706 AK017551 Nol8 1423415_at 4.463 BB110067 Gpr83 1452411_at 4.692 BG966295 Lrrc1 1441928_x_at 4.461 BB139475 Ell 1425680_a_at 4.692 AF110396 Btrc 1428950_s_at 4.461 AK017551 Nol8 1417341_a_at 4.692 NM_025800 Ppp1r2 1431230_a_at 4.461 Z74630 Btbd9 1430617_at 4.68 BB238604 Oip5 1424475_at 4.459 BI157430 Camkk2 1434594_at 4.679 BB497449 Dnahc1 1448687_at 4.457 NM_026125 C1qdc2 1428095_a_at 4.663 AK004920 Tmem24 1428864_at 4.456 AK017419 5530400B01Rik 1437378_x_at 4.45 BB224405 Scarb1 1460717_at 4.252 AF042180 Tspyl1 1423785_at 4.448 BE995700 Egln1 1437579_at 4.246 C77054 Nek2 1442370_at 4.436 BB267006 1431920_a_at 4.234 AK008082 2010004A03Rik 1424175_at 4.433 BC017689 Tef 1442708_at 4.225 BB504418 1460718_s_at 4.433 AF192558 Mtch1 1424759_at 4.224 BC017528 Arrdc4 1436100_at 4.431 AV347995 Sh2d5 1441786_at 4.217 AW048005 1440479_at 4.431 BB821623 Cbx4 1430037_at 4.216 AK017836 Snx27 1451696_at 4.43 BC004695 Zfp64 1426978_at 4.213 AW682368 Klhl2 1426585_s_at 4.429 BM209765 Mapk1 1455555_at 4.212 BF224468 AV071699 1439548_at 4.428 BB390705 Rap2b 1443888_at 4.206 BB426608 1436754_at 4.42 BB360523 AI839735 1451507_at 4.205 BB280300 Mef2c 1433707_at 4.419 BB430205 Gabra4 1435441_at 4.204 BB336053 Ablim2 1451285_at 4.416 AF224264 Fus 1435845_at 4.2 BB764479 2900006N09Rik 1439422_a_at 4.414 AV048291 C1qdc2 1415985_at 4.199 NM_133953 Sf3b3 1456755_at 4.388 AI467545 2310001H13Rik 1448747_at 4.194 AF441120 Fbxo32 1416537_at 4.386 NM_133930 Creld1 1421897_at 4.194 AI385733 Elk1 1423478_at 4.377 BF660388 Prkcb1 1416402_at 4.184 AV382118 Abcb10 1424480_s_at 4.374 BC026151 Akt2 1426465_at 4.175 BG066219 Dlgap4 1422751_at 4.374 NM_011599 Tle1 1455771_at 4.174 BB467799 Bzrap1 1417456_at 4.372 NM_010322 Gnpat 1430128_a_at 4.169 AK002562 Dp1l1 1418168_at 4.371 BB223737 Zcchc14 1437865_at 4.168 AW546433 Spata13 1448656_at 4.365 NM_007581 Cacnb3 1427300_at 4.167 D49658 Lhx8 1426241_a_at 4.358 AB030906 Scmh1 1440258_at 4.165 BE951512 Kcnq2 1423049_a_at 4.352 AK002271 Tpm1 1426012_a_at 4.147 BC010499 2610301G19Rik 1426900_at 4.35 AV293164 Jmjd1c 1434805_at 4.147 BG063049 Mllt1 1458443_at 4.345 BE986758 Mect1 1417524_at 4.144 NM_009920 Cnih2 1426822_at 4.344 BM201133 Rhot2 1455772_at 4.141 BB428874 Pgr 1417461_at 4.335 NM_007598 Cap1 1424384_a_at 4.14 BC006765 Znrf1 1427674_a_at 4.33 D29763 Sez6 1416448_at 4.137 NM_025922 Itpa 1450835_a_at 4.329 NM_020014 Gfra4 1435204_at 4.129 BG065273 Hrmt1l4 1454844_at 4.325 AW049955 Gpr24 1422527_at 4.123 NM_010386 H2-DMa 1421962_at 4.324 A1664344 Dnajb5 1455548_at 4.122 BB314358 Dlgap4 1424645_at 4.32 BB795102 Tnrc6c 1423694_at 4.121 BC006935 Kctd10 1455080_at 4.317 BB375209 Ppp1r16b 1428872_at 4.117 AW495537 4121402D02Rik 1423228_at 4.317 BG066773 B4galt6 1421175_at 4.116 NM_008666 Myt1l 1423200_at 4.317 U22016 Ncor1 1426897_at 4.115 AV122997 Rcc2 1417393_a_at 4.314 NM_026125 C1qdc2 1434762_at 4.114 BF457736 A730041015Rik 1418467_at 4.305 NM_025891 Smarcd3 1436005_at 4.111 BQ174136 Sfrs14 1420427_a_at 4.296 NM_133941 Dhx32 1420738_at 4.109 NM_016793 Zfp98 1422407_s_at 4.294 NM_008284 Hras1 1423699_at 4.107 BC003900 D15Ertd785e 1425929_a_at 4.289 AF249668 Rnf14 1438663_at 4.106 BF730196 Bat2d 1451908_a_at 4.287 BC005766 Sec14l1 1435674_at 4.101 BB351953 Rhobtb2 1415998_at 4.284 NM_011694 Vdac1 1420718_at 4.093 NM_011856 0dz2 1428937_at 4.281 BI080417 Atp2b1 1454960_at 4.09 BI646741 Smad3 1417062_at 4.279 NM_026034 2810037C14Rik 1447947_at 4.089 BM220786 Zfyve16 1428148_s_at 4.271 BB203098 Coro7 1435598_at 4.089 BE996371 Shc2 1441369_at 4.27 BB329116 1416397_at 4.077 BB474887 Mesdc1 1431074_a_at 4.268 BI654600 Pitpnc1 1433526_at 4.076 BM942651 Klhl8 1441815_at 4.266 AI851453 AI851453 1460279_a_at 4.074 AF043219 Gtf2i 1442562_at 4.263 BB346556 D630040G17Rik 1453972_x_at 4.072 AK005207 Pparbp 1459920_at 4.262 AA163908 4933415E08Rik 1425679_a_at 4.066 AF109769 Mapk8ip1 1458399_at 4.261 AI413999 Lrrc3 1448553_at 4.063 NM_080728 Myh7 1448877_at 4.26 NM_010054 Dlx2 1429738_at 4.057 AK013847 Myt1l 1439866_at 4.255 BQ173950 Parc 1439497_at 4.056 BG065013 4933415E08Rik 1423522_at 4.254 BB811478 Npm3 1428443_a_at 4.048 AK005063 Rap1ga1 1449214_a_at 4.252 BC025160 Opa1 1443694_at 4.045 BB794177 Rgs20 1421340_at 4.043 NM_008580 Map3k5 1428259_at 3.91 AK010185 2310075M15Rik 1430640_a_at 4.039 BI695530 Prkar2b 1440207_at 3.907 BI155142 AI1505034 1416605_at 4.036 BC024944 Nola2 1450853_at 3.904 AU045006 Tle4 1435206_at 4.036 BB767169 Slc24a4 1427579_at 3.904 BE653576 Rhbdl4 1428147_at 4.035 BB203098 Coro7 1426973_at 3.903 AK004377 Gpr153 1446312_at 4.034 BB273243 1439888_at 3.902 BB271581 1429174_at 4.032 AK014304 Wdr34 1427618_at 3.901 BQ176417 Cdh9 1428508_at 4.029 AK004526 1810061M12Rik 1435844_at 3.896 BB164247 A330009N23Rik 1456280_at 4.022 BG067086 Clspn 1426644_at 3.886 BC002196 Tbc1d20 1434563_at 4.021 BM207149 Rps6kc1 1436563_at 3.882 AV277283 4932441J04Rik 1440870_at 4.018 BB356786 Prdm16 1436381_at 3.88 BQ175774 Dlgap3 1454835_at 4.014 AV340515 Epm2aip1 1460552_at 3.878 AI327392 Ascc3l1 1422293_a_at 4.008 NM_134112 Kctd1 1454609_x_at 3.878 BB770958 6430527G18Rik 1452063_at 4.007 AK010736 2410081M15Rik 1429715_at 3.878 AK010380 Ppp2r2a 1425600_a_at 4.007 AW049748 Plcb1 1452911_at 3.872 AK017680 Spred1 1455242_at 3.999 BM220880 Foxp1 1455956_x_at 3.864 AV310588 Ccnd2 1456930_at 3.999 BE989461 Camsap1 1416981_at 3.864 AI462296 Foxo1 1433499_at 3.998 BF585125 2010005J08Rik 1456838_at 3.863 BE995608 LOC237403 1417216_at 3.997 NM_138606 Pim2; 1417749_a_at 3.862 NM_009386 Tjp1 LOC229005 1434969_at 3.859 BB381558 1440487_at 3.995 BE824778 Dcc 1421107_at 3.859 NM_021420 Stk4 1437400_at 3.992 BB309512 Nedd4l 1454875_a_at 3.857 BF011461 Rbbp4 1434904_at 3.991 AW321867 Hivep2 1422807_at 3.856 NM_007480 Arf5 1439867_at 3.987 BB079377 Ubox5 1460436_at 3.856 BI652065 Ndst1 1420961_a_at 3.983 NM_028582 Ivns1abp 1434082_at 3.851 BM243464 Pctk2 1421498_a_at 3.982 NM_023450 2010204K13Rik 1436513_at 3.85 BM208226 3526402J09Rik 1451008_at 3.982 BB360510 St8sia3 1452844_at 3.844 AK009674 Pou6f1 1429718_at 3.981 BB782729 Slitrk5 1422590_at 3.844 NM_007668 Cdk5 1451098_at 3.98 BC010524 Pcoln3 1453915_a_at 3.841 AK012071 Slc37a3 1433642_at 3.974 BB384173 Arfrp2 1443612_at 3.84 AW123199 1456785_at 3.973 BQ175889 Crsp2 1419080_at 3.831 NM_010275 Gdnf 1422255_at 3.97 NM_021275 Kcna4 1460568_at 3.819 BI156583 Trim46 1429104_at 3.964 AK012581 0610025L06Rik 1426643_at 3.815 AK012072 Elp3 1447642_x_at 3.96 AV354897 Dmwd 1435618_at 3.814 BB473446 Pnma2 1420999_at 3.958 AI448404 Cnot4 1422485_at 3.814 AK004804 Smad4 1459994_x_at 3.955 AV027486 Trfr2 1452057_at 3.807 BG801851 Actr1b 1455820_x_at 3.955 BB138434 Scarb1 1435125_at 3.804 BB303627 BB120497 1456606_a_at 3.949 AV259240 Chst11; Phactr1 1416122_at 3.801 NM_009829 Ccnd2 1455365_at 3.948 BB426483 Cdh8 1428839_at 3.8 AK005105 Wdr53 1455191_x_at 3.948 BB822856 Pip5k1b 1426770_at 3.799 AJ416473 Pex5 1440177_at 3.948 BM899529 9.63E+17 1452940_x_at 3.798 AI595920 Pitpnc1 1443073_at 3.945 BB355954 LOC545681 1441867_x_at 3.796 AI480494 4930534B04Rik 1422335_at 3.942 NM_007418 Adra2c 1423195_at 3.796 BM208582 Hiat1 1460223_a_at 3.942 NM_013514 Epb4.9 1456444_at 3.795 BB126849 Fbxo41 1433743_at 3.937 BG075820 Dach1 1433811_at 3.794 BG245272 Mllt6 1426785_s_at 3.931 BI411560 Mgll 1433747_at 3.793 BQ176475 Lnpep 1415976_a_at 3.93 AU080787 Carhsp1 1417305_at 3.793 NM_007463 Apeg1 1452938_at 3.924 BQ174247 C030032C09Rik 1457077_at 3.791 BB306048 1418323_at 3.921 BM232562 Fem1b 1428853_at 3.79 BG071079 Ptch1 1424132_at 3.92 BC011083 Hras1 1428852_at 3.788 AK018140 Dock3 1426487_a_at 3.918 BB092954 Rbbp6 1424150_at 3.788 BC024955 Gdpd5 1426108_s_at 3.917 AY094172 Cacnb1 1421191_s_at 3.786 NM_053187 Gopc 1426383_at 3.917 BF303057 Cry2 1427468_at 3.782 M81483 Ppp3cb 1428914_at 3.917 AK009333 2310014D11Rik 1418080_at 3.782 AW125856 B4galt2 1455279_at 3.911 BG070552 Gm1060 1453689_at 3.781 AK013325 Fance 1460610_at 3.91 BG143491 9430057O19Rik 1419234_at 3.781 BG070273 Helb 1460210_at 3.78 NM_013630 Pkd1 1428716_at 3.656 BG076082 Pex1 1428173_at 3.777 AK005562 Eml2 1451998_at 3.653 BC024597 4930485D02Rik 1435547_at 3.776 BQ175722 Mkl2 1431717_at 3.653 AK014386 3526401B18Rik 1428892_at 3.773 AK004331 Ppil1 1454685_at 3.649 BQ177047 1456766_at 3.773 BB036966 C330001K17Rik 1423269_a_at 3.649 BB663717 Nedd4l 1439662_at 3.771 BM124609 Homer1 1450910_at 3.647 AV261931 Cap2 1428786_at 3.77 BM238906 Nckap1l 1416535_at 3.644 BC003746 Mcrs1; 1445194_at 3.77 BB355006 Cnksr2 LOC433780 1438266_at 3.77 BB764453 Thsd6 1417032_at 3.644 AF296657 Ube2g2 1428926_at 3.769 AK003388 1110003O08Rik 1421312_a_at 3.643 NM_010630 Kifc2 1427071_at 3.767 BC003960 Fbxo42 1460009_at 3.639 BB667115 Ier5 1452149_at 3.766 AU067784 Ube3b 1416550_at 3.639 BB320416 Slc35b4 1422737_at 3.765 NM_008679 Ncoa3 1449168_a_at 3.638 BC003735 Akap2 1423842_a_at 3.764 AF305730 Rnf41 1418639_at 3.631 NM_011359 Sftpc 1426972_at 3.763 AK009425 Sec24d 1435504_at 3.63 BM217861 Rsnl2 1454659_at 3.762 BG069699 Dctd 1435019_at 3.629 BB532141 Atxn7l3 1423519_at 3.759 BE457744 2210412D01Rik 1423673_at 3.628 BC023053 Ldoc1l 1445359_at 3.756 BE993830 Adcy1 1437842_at 3.626 BB311508 Plcxd1 1447696_x_at 3.754 BB091427 Adcy5 1433787_at 3.623 AI841091 B230343H07Rik 1429228_at 3.754 BE980134 4930534B04Rik 1451568_at 3.62 BC025603 A630054L15Rik 1421817_at 3.754 AK019177 Gsr 1452624_at 3.618 BM900077 Lrrtm1 1440741_at 3.751 BB829587 Htr1d 1450520_at 3.617 NM_019430 Cacng3 1457919_at 3.739 BB446049 D030054H15Rik 1433699_at 3.617 BM241351 Tnfaip3 1456601_x_at 3.738 AV002675 Fxyd2 1433468_at 3.614 BB770958 6430527G18Rik 1452718_at 3.736 BG073486 Edd1 1418016_at 3.614 BI689507 Pum2 1442883_s_at 3.734 BI413749 D10Bwg1364e 1419248_at 3.612 AF215668 Rgs2 1417844_at 3.734 NM_026119 Med4 1429021_at 3.608 AK013481 Epha4 1420965_a_at 3.732 BM120053 Enc1 1427954_at 3.606 BC028785 BC048403 1455023_at 3.728 BB171181 N28178 1449932_at 3.605 NM_027874 Csnk1d 1452913_at 3.725 AV337888 Pcp4l1 1454985_at 3.604 BI872009 D030051N19Rik 1453428_at 3.72 AK012380 2700045P11Rik 1451722_s_at 3.602 BF160651 Smyd5 1432813_at 3.718 AK013749 2900064F13Rik 1429122_a_at 3.6 AK006658 1700040I03Rik 1433924_at 3.709 BM200248 Peg3 1424317_at 3.6 BC018167 Slc25a19 1438913_x_at 3.707 AW322533 Hdgfrp2 1447124_at 3.599 BE634581 1438213_at 3.705 BB128962 A830018L16Rik 1437989_at 3.598 BB357157 Pde8b 1429201_at 3.705 AK013508 Cyld 1448374_at 3.595 AK005130 Med28 1416125_at 3.699 U16959 Fkbp5 1424044_at 3.594 BC007145 Jmjd2b 1457213_a_at 3.698 AV276089 Dgkh 1434832_at 3.59 BB364488 Foxo3a 1416106_at 3.695 NM_029571 Kti12 1436483_at 3.589 BM116113 Myt1l 1442099_at 3.695 BM227490 Usp31 1453324_at 3.588 BF459399 6330509M23Rik 1421860_at 3.689 BG065300 Clstn1 1437750_at 3.586 BI133257 2310037P21Rik 1450366_at 3.689 NM_007545 Hrk 1443542_at 3.582 BB767151 1433777_at 3.688 BB152370 L3mbtl2 1429392_at 3.58 BI558553 Wdr40a 1416953_at 3.685 NM_010217 Ctgf 1456144_at 3.58 AW537064 Nav3 1426009_a_at 3.685 BC003763 Pip5k1b 1457456_at 3.577 AI481735 Map3k10 1422453_at 3.674 NM_138659 Prpf8 1419056_at 3.577 AF038538 Rtn2 1445854_at 3.674 BB380166 C230004F18Rik 1429832_at 3.575 AK014665 Ppih 1452438_s_at 3.672 BE632382 Taf4a 1458351_s_at 3.572 BB428573 Klhl2 1426069_s_at 3.671 BC016100 Slc7a4 1428936_at 3.572 BI080417 Atp2b1 1424047_at 3.67 BC016218 Dera 1418173_at 3.571 NM_133730 4631426H08Rik 1433987_at 3.666 AV153557 Hpcal4 1454728_s_at 3.569 AW610650 Atp8a1 1454700_at 3.665 BB093349 Lrfn4 1422474_at 3.568 BM246564 Pde4b 1448498_at 3.663 NM_019924 Rps6ka4 1451106_at 3.565 BC025499 Rbm21 1416863_at 3.663 NM_022419 Abhd8 1422677_at 3.564 AK002443 Dgat2 1428222_at 3.66 AK018179 Dcamkl2 1423973_a_at 3.562 BC024935 Arf3 1430413_at 3.66 AK018228 6330540D07Rik 1422799_at 3.562 AK019427 Bat2 1446681_at 3.562 AV327329 BB086117 1437580_s_at 3.453 C77054 Nek2 1451506_at 3.557 BB280300 Mef2c 1460262_a_at 3.451 NM_026091 1700037H04Rik 1429678_at 3.557 AK017758 5730508B09Rik 1434339_at 3.45 AW548221 Fnbp1l 1457361_at 3.555 BB628626 C630007C17Rik 1428777_at 3.447 AK017680 Spred1 1424117_at 3.552 BC024937 BC056474 1418743_a_at 3.447 NM_021344 MGI: 1930803 1436142_at 3.55 BM218877 3526401B18Rik 1450040_at 3.444 M93954 Timp2 1452467_at 3.549 BQ042988 Mmab 1455033_at 3.444 BB325849 B430201A12Rik 1434830_at 3.547 AV228517 Mxd1 1427930_at 3.444 BG063905 Pdxk 1428327_at 3.547 BB010301 2310001H13Rik 1457256_x_at 3.441 BB530125 Ptch2 1423442_a_at 3.545 BE854125 Fbxw2 1454092_a_at 3.441 AK017176 Gtf2h3 1423330_at 3.543 AK006149 Ensa 1427993_at 3.434 AI852705 Rufy2 1433621_at 3.536 BB334979 Wdr41 1448554_s_at 3.432 NM_080728 Myh6 1455395_at 3.536 BB446073 4933425A18Rik 1448445_at 3.431 AB030038 Acp6 1425180_at 3.535 AV344708 Sgip1 1426786_s_at 3.431 BM195397 Dhx38 1427091_at 3.532 BC025488 AI481105 1455401_at 3.428 AW061083 Camkk2 1423395_at 3.53 BM021706 Tsnax 1420694_a_at 3.425 BB522228 Dach1 1437921_x_at 3.53 AW744723 Zfp516 1416087_at 3.419 NM_007457 Ap1s1 1419577_at 3.524 NM_133999 A530089I17Rik 1449502_at 3.418 NM_010021 Dazl 1434154_at 3.523 BQ177107 Kctd13 1419583_at 3.416 NM_007625 Cbx4 1416292_at 3.521 NM_007452 Prdx3 1425415_a_at 3.414 U75214 Slc1a1 1438478_a_at 3.52 AI313926 Ppp3ca 1434902_at 3.413 BB163668 Rnf157 1434415_at 3.519 BB797871 1454827_at 3.41 BG070415 Pogz 1449494_at 3.514 AY026947 Rab3c 1435169_at 3.409 BB431047 A930001N09Rik 1424503_at 3.511 BC006596 Rab22a 1420533_at 3.409 AK004815 Gucy1a3 1455247_at 3.508 BB767281 Amotl1 1422431_at 3.408 NM_053201 Magee1 1417349_at 3.507 NM_019788 Pldn 1437214_at 3.408 BB667279 Lrrtm4 1452269_at 3.506 AF026489 Spnb3 1417829_a_at 3.406 NM_134050 Rab15 1460623_at 3.506 BB753881 Scap2 1455865_at 3.404 BB468410 Insm1 1429330_at 3.504 AK013727 Gabra4 1437980_at 3.404 BB814947 9130230N09Rik 1425580_a_at 3.504 BC024675 Pik3c3 1451273_x_at 3.404 BC025546 BC025546 1418895_at 3.502 NM_018773 Scap2 1434720_at 3.403 BF320240 9530033F24Rik 1428642_at 3.497 AK018094 Slc35d3 1419834_x_at 3.4 AW491150 Mark1 1428550_at 3.496 AK007503 1810015A11Rik 1434531_at 3.397 BB400317 GnT-IX 1452234_s_at 3.493 BF457957 D16Bwg1494e 1435233_at 3.396 BM234716 Ncoa2 1428600_at 3.49 AK014241 3110068G20Rik 1424199_at 3.396 AW537349 Seh1l 1448745_s_at 3.487 NM_008508 Lor 1438039_at 3.396 BE630599 Hectd1 1434603_at 3.485 BG074645 Thrap2 1455102_at 3.393 BB213860 D330037H05Rik 1419522_at 3.484 NM_026021 Zmynd19 1435549_at 3.392 BI685685 Trpm4 1444363_at 3.477 BB269445 1416013_at 3.392 NM_011116 Pld3 1423978_at 3.476 BC025837 Sbk1 1438272_at 3.391 BB315073 Csmd3 1436013_at 3.476 AI852434 C230098I05Rik 1453485_s_at 3.391 AK016897 1110005A03Rik 1433558_at 3.476 BM231046 Dab2ip 1428924_at 3.39 AU016306 Mocs3 1424320_a_at 3.474 BC008598 Traf7 1436260_at 3.387 BB131085 1434025_at 3.469 BG069607 1436354_at 3.386 BE992423 Dzip1l 1438421_at 3.469 BI134275 Pyrl1 1436763_a_at 3.386 A1267126 Klf9 1429588_at 3.468 AA509870 2810474O19Rik 1421662_a_at 3.384 NM_030254 Tusc3 1450436_s_at 3.466 AI664344 Dnajb5 1421037_at 3.384 BG070037 Npas2 1416918_at 3.465 NM_016747 Dlgh3 1438662_at 3.382 AW123234 LOC433810 1429618_at 3.465 BM119209 Cyld 1431076_at 3.382 BG976853 Add2 1449843_at 3.465 BG071333 St8sia2 1456863_at 3.381 AI385584 Epha4 1455265_a_at 3.464 BB100249 Rgs16 1421399_at 3.38 NM_016889 Insm1 1450863_a_at 3.462 BQ174703 Dcamkl1 1452292_at 3.379 AV271093 Ap2b1 1417794_at 3.461 NM_019831 Zfp261 1425132_at 3.377 AF448840 Neto1 1423583_at 3.46 AK005041 Fem1a 1437231_at 3.374 AV246497 Slitrk6 1457729_at 3.458 AW492805 1423777_at 3.374 AK006800 Usp20 1428585_at 3.456 BE853286 Actn1 1451751_at 3.372 AF335325 Ddit4l 1439106_at 3.371 AV320128 Zfp462 1447301_at 3.338 BG228875 Gm258 1421027_a_at 3.37 AI595932 Mef2c 1417890_at 3.336 NM_020271 Pdxp 1436085_at 3.37 BB361974 Zbtb34 1437918_at 3.335 AV374644 4930539E08Rik 1434819_at 3.369 BB709312 St6gal2 1434228_at 3.334 AV255921 Ppm2c 1441572_at 3.369 BB264612 C030036D22Rik 1432091_a_at 3.333 AK007455 Rutbc3 1453200_at 3.368 AK013909 Rai1 1417375_at 3.332 AW491660 Tuba4 1418181_at 3.366 AK014601 Ptp4a3 1442093_at 3.331 AU040368 1448141_at 3.364 NM_029101 1110014J01Rik 1451533_at 3.328 BC022687 BC022687 1452647_a_at 3.361 AK011199 Dph2 1427212_at 3.328 BC027377 Mapkap1 1455670_at 3.36 BQ175884 Rbms3 1458967_at 3.326 BM939341 1423367_at 3.36 AK004683 Wnt7a 1449063_at 3.326 BC009024 Sec22l1 1423832_at 3.359 BB756794 Prkag2 1455029_at 3.325 BB342219 Kif21a 1453260_a_at 3.355 AK010380 Ppp2r2a 1453268_at 3.322 AV224102 5730409G07Rik 1436396_at 3.355 AV291191 D430033N04Rik 1422594_at 3.318 BC004090 5730470L24Rik 1419879_s_at 3.354 AA960166 Trim25 1455289_at 3.317 BQ174304 Ankrd13b 1421943_at 3.351 M92420 Tgfa 1423344_at 3.316 AK010968 Epor 1415753_at 3.35 BC005632 D10Bwg1364e 1426416_a_at 3.316 AV216410 Yipf4 1434476_at 3.349 BM119896 Mect1 1438434_at 3.313 AV349116 Arhgap11a 1418174_at 3.347 BC018323 Dbp 1456760_at 3.311 BB342676 1451888_a_at 3.341 AB025413 Odz4 1451207_at 3.31 BC023022 Cbara1 ProbeSymbol = Affymetrix probe identification corresponding to gene symbol; FEN = fold enrichment when compared to reference sample; Genbank = Genbank identification; GeneSymbol = official gene symbol.

TABLE 19 Top 1,000 brain stem cholinergic motor neuron (Chat) translated mRNAs enriched when compared to a reference sample ProbeSymbol = Affymetrix probe identification corresponding to gene symbol; FEN = fold enrichment when compared to reference sample; Genbank = Genbank identification; GeneSymbol = official gene symbol. ProbeSymbol FEN Genbank GeneSymbol ProbeSymbol FEN Genbank GeneSymbol 1422203_at 1618.00 NM_021712 Slc18a3 1416762_at 52.76 NM_009112 S100a10 1422639_at 1463.00 NM_054084 Calcb 1448182_a_at 51.25 NM_009846 Cd24a 1422530_at 385.40 NM_013639 Prph1 1436520_at 50.88 BB378317 AI450948 1452004_at 363.20 AF330212 Calca 1417013_at 50.33 AF250139 Hspb8 1417788_at 192.50 NM_011430 Sncg 1455439_a_at 48.08 AI642438 Lgals1 1452243_at 190.40 BB282273 Kcnj14 1427357_at 46.95 AK008793 Cda 1438042_at 177.90 AV332957 Shox2 1418787_at 45.08 NM_010776 Mbl2 1426511_at 149.60 AK004703 Susd2 1453351_at 44.45 AK020409 Tbx20 1455907_x_at 124.40 BB059017 Phox2b 1427351_s_at 43.40 BB226392 Igh-6 1438967_x_at 120.00 AV259665 Amhr2 1421359_at 41.03 NM_009050 Ret 1436461_at 115.40 BQ175922 G3bp ″AFFX-r2- 1416034_at 108.50 NM_009846 Cd24a Bs-dap-3_at″ 40.73 AFFX-r2-Bs- 1433578_at 103.50 BE824538 E130304D01 dap-3 1422644_at 98.31 NM_015825 Sh3bgr 1457021_x_at 39.81 AV209206 Amhr2 1451440_at 90.54 AF311699 Chodl 1449106_at 39.51 NM_008161 Gpx3 1420388_at 86.56 NM_008939 Prss12 1453127_at 38.86 AK009235 Ppm1j 1439609_at 85.66 BB536410 1423856_at 38.80 BC003896 Popdc3 1425964_x_at 85.58 U03561 Hspb1 1443906_at 38.28 BE686894 Daf1 1449254_at 83.13 NM_009263 Spp1 1449271_a_at 38.26 NM_019487 Hebp2 1443372_at 80.13 BB417900 Chat 1436239_at 37.11 BB328706 Slc5a5 1455893_at 78.48 BG067392 Rspo2 1448816_at 35.41 NM_008968 Ptgis 1419200_at 74.35 NM_022007 Fxyd7 1427989_at 35.01 AF503863 Amhr2 1421799_at 69.66 NM_011910 Uts2 1423557_at 34.83 BF537076 Ifngr2 1460668_at 62.98 NM_010253 Gal 1436359_at 34.72 BF471533 Ret 1422943_a_at 61.77 NM_013560 Hspb1 1423693_at 34.64 BC011218 Ela1 1454660_at 59.01 BB367590 1100001E04Rik 1419091_a_at 34.50 NM_007585 Anxa2 1419573_a_at 58.02 NM_008495 Lgals1 1415983_at 34.34 NM_008879 Lcp1 1418917_at 52.82 NM_019487 Hebp2 1418517_at 34.07 NM_008393 Irx3 1437137_at 33.87 AV280875 6430550H21Rik 1422825_at 20.10 NM_013732 MGI:1351330 ″AFFX- 1421223_a_at 20.05 NM_013471 Anxa4 DapX-3_at″ 31.89 AFFX-DapX-3 1420984_at 19.96 AF114437 Pctp 1437502_x_at 31.73 BB560574 Cd24a 1439015_at 19.88 AV221299 Gfra1 1454171_x_at 31.57 AK020608 9530053H05Rik 1428664_at 19.81 AK018599 Yip 1421428_at 31.11 NM_022025 Slc5a7 1439086_at 19.60 BB278168 A930009L07Rik 1418762_at 30.97 NM_010016 Daf1 1439885_at 19.35 AA051236 Hoxc5 1448291_at 30.74 NM_013599 Mmp9 1424531_a_at 19.22 BC010807 Tcea3 1446681_at 30.58 AV327329 BB086117 1424176_a_at 19.16 BE628614 Anxa4 1436503_at 30.52 BF302511 BC048546 1438444_at 18.99 AV381832 A230091H23Rik 1448160_at 30.41 NM_008879 Lcp1 1418176_at 18.97 AV290079 Vdr 1453855_at 30.02 BG295762 1810057P16Rik 1423427_at 18.96 AI323434 Adcyap1 1423947_at 29.93 BC024615 1110008P14Rik 1450869_at 18.84 AI649186 Fgf1 1428536_at 29.46 AK015001 Kcng4 1452968_at 18.50 AK003674 Cthrc1 1422586_at 28.71 NM_021306 Ecel1 1434779_at 18.48 BQ175551 Cbln2 1456532_at 28.49 BB428671 Pdgfd 1432198_at 18.14 AK018172 1418352_at 28.42 BC012682 Hsd17b2 1427975_at 18.09 AK008807 2210403B10Rik 1421862_a_at 28.31 AK018783 Vamp1 1416298_at 17.83 NM_013599 Mmp9 1416357_a_at 28.06 NM_023061 Mcam 1423281_at 17.70 BM115022 Stmn2 1418415_at 27.45 NM_008268 Hoxb5 1453638_at 17.69 AK018520 9030420J04Rik 1423560_at 27.34 AI838010 Nell2 1449545_at 17.67 NM_008005 Fgf18 1422860_at 26.84 NM_024435 Nts 1451814_a_at 17.61 AF061972 Htatip2 1452913_at 26.70 AV337888 Pcp4l1 1453593_at 17.42 BG066866 1700110N18Rik 1450610_at 26.27 NM_021290 Ucn 1447791_s_at 17.37 AV230778 Gna14 1434442_at 25.66 BB667844 D5Ertd593e 1429154_at 17.30 BG069340 Slc35f2 ″AFFX-r2- 1437762_at 17.18 BB130995 Rab39 Bs-dap-M_at″ 24.43 AFFX-r2-Bs- 1430596_s_at 17.13 BG066866 1700110N18Rik dap-M 1426063_a_at 17.06 U10551 Gem 1455919_at 24.09 AV330588 1422452_at 16.97 NM_013863 Bag3 1441778_at 23.92 BB171354 Adcyap1 1435790_at 16.91 BG864960 Olfm2 1444165_at 23.75 BB530994 E030012M19Ri 1450723_at 16.81 BQ176915 Isl1 k 1417960_at 16.61 NM_007755 Cpeb1 1452010_at 23.39 AF472588 Chrna3 1457048_at 16.38 BB526932 Gpr103 1451515_s_at 23.24 BC010799 Glyat 1440677_at 16.34 BB308744 LOC245305 1424900_at 22.65 BC025599 Slc29a4 1416713_at 16.34 NM_026481 2700055K07Rik ″AFFX- 1460255_at 16.17 NM_033622 Tnfsf13b DapX-M_at″ 22.56 AFFX-DapX- 1422852_at 16.02 NM_019686 Cib2 M 1420256_x_at 15.90 AV354142 1422635_at 22.40 NM_009599 Ache 1439578_at 15.80 BB713457 Lsm11 1435184_at 22.18 BG066982 B430320C24Rik 1455642_a_at 15.79 AI844703 Tspan17 1445281_a_at 21.58 BB309504 B230311B06Rik 1418901_at 15.77 NM_009883 Cebpb 1417312_at 21.31 AK004853 Dkk3 1428544_at 15.64 BE689422 0610007L01Rik 1436141_at 21.14 BM934616 2610510L01Rik 1456247_x_at 15.57 AV166926 Plp2 1436332_at 20.96 BB755506 Hspb6 1424249_a_at 15.48 BC024535 Arhgap9 ″AFFX-r2- 1459750_s_at 15.27 AU015577 Gpr123 Bs-thr- 20.94 AFFX-r2-Bs- 1452246_at 15.22 U58888 Ostf1 3_s_at″ thr-3 1424927_at 15.10 BC025083 Glipr1 1460299_at 20.73 NM_019944 Hlxb9 1436622_at 15.02 AW492241 Iqsec2 1449899_at 20.42 NM_130455 Grin3b 1426937_at 15.01 AK018128 6330406I15Rik 1420385_at 20.25 NM_008137 Gna14 1459890_s_at 14.98 C79326 1110008P14Rik 1427747_a_at 20.23 X14607 Lcn2 1422118_at 14.92 NM_023485 Sync 1442608_at 20.21 AV071586 E030012M19Ri 1417956 at 14.86 NM_007702 Cidea k 1425245_a_at 14.81 BC019741 Rgs11 ″AFFX- 1429696_at 14.81 BE946247 Gpr123 ThrX-3 at″ 20.17 AFFX-ThrX-3 1422720_at 14.68 BQ176915 Isl1 1417228_at 20.11 AF084459 Capn1 1427900_at 14.53 BC028795 Pip5kl1 1424443_at 14.46 AV378394 Tm6sf1 1453591_at 12.38 AK017626 5730437N04Rik 1449155_at 14.40 NM_026190 Polr3g 1457008_at 12.36 AW456706 Chrnb4 1449571_at 14.38 M59811 Trhr 1434493_at 12.35 BG092222 1810022K09Rik 1451232_at 14.34 U89772 Cd151 1449429_at 12.31 NM_016863 Fkbp1b 1416935_at 14.31 NM_011706 Trpv2 1429732_at 12.24 AK016334 Ppp1r1c 1456925_at 14.22 AW490413 P2rxl1 1437914_at 12.20 BB104097 E2f6 1450791_at 14.19 NM_008726 Nppb 1423426_at 12.19 BB018522 1300012G16Rik 1433626_at 14.17 BB826296 Plscr4 1450885_at 12.12 BB446076 Dffa 1429385_at 14.17 BI082535 Wdr68 1415978_at 12.09 NM_023279 Tubb3 1459300_at 14.14 BB226459 1423136_at 12.04 AI649186 Fgf1 1448259_at 14.02 BI452727 Fstl1 1427854_x_at 12.02 AF047377 Hspb1 1459921_at 13.98 BQ042959 Nxph1 1456468_x_at 12.00 BB189996 1110012N22Rik 1427853_a_at 13.97 AF047377 Hspb1 1416656_at 11.95 NM_033444 Clic1 1424692_at 13.85 BC004753 2810055F11Rik 1428751_at 11.95 AK005771 Pacrg 1449161_at 13.83 NM_007902 Edn2 1448669_at 11.94 AK004853 Dkk3 1444060_at 13.80 BB497990 Bcas3 1418571_at 11.94 NM_013749 Tnfrsf12a 1451346_at 13.74 BG075139 Mtap 1424487_x_at 11.93 BB284199 Txnrd1 1457779_at 13.64 AV013305 1110046J04Rik 1426606_at 11.88 BB426194 Crtac1 1449368_at 13.64 NM_007833 Dcn 1429953_at 11.85 AK008705 2210011C24Rik 1453111_a_at 13.61 AK019396 D11Ertd333e 1429934_at 11.81 AK015678 4930502E18Rik 1455239_at 13.57 BM116861 6330512M04Rik 1416431_at 11.67 NM_026473 Tubb6 1447493_at 13.55 AI413840 A530088H08Rik 1438763_at 11.64 BB016751 1415832_at 13.54 NM_007429 Agtr2 1448660_at 11.58 NM_008113 Arhgdig 1452451_at 13.53 AF240460 MGI:1929597 1418780_at 11.52 NM_018887 Cyp39a1 1424680_at 13.50 BC025893 BB146404 1441957_x_at 11.51 AV216663 2410076I21Rik 1419810_x_at 13.50 AU043488 Arhgap9 1452508_x_at 11.50 BC021465 A230083G16Rik 1449130_at 13.50 NM_007639 Cd1d1 1442180_at 11.49 BB382040 Dleu7 1449832_at 13.48 NM_028570 1700091H14Rik 1443684_at 11.48 BE956557 1418610_at 13.47 NM_080853 Slc17a6 1418649_at 11.39 BB284358 Egln3 1425051_at 13.46 AK010892 Isoc1 1453755_at 11.38 BM951302 Lsm11 1421973_at 13.44 BE534815 Gfra1 1426221_at 11.37 BC004727 Loh11cr2a 1416080_at 13.41 NM_009614 Adam15 1442631_at 11.34 BM935306 1427512_a_at 13.32 X84014 Lama3 1451322_at 11.27 BC024580 2310016A09Rik 1451636_at 13.31 BC022726 Ankrd33 1436238_at 11.23 AI841179 Lgi3 1422545_at 13.28 NM_009324 Tbx2 1434341_x_at 11.20 BF681728 1110020P15Rik 1434340_at 13.26 BF681728 1429231_at 11.18 AK015078 1426540_at 13.21 BF168366 2310067E08Rik 1451956_a_at 11.15 AF226605 Oprs1 1417291_at 13.20 L26349 Tnfrsf1a 1450230_at 11.10 NM_138582 D17H6S56E-3 1428384_at 13.18 AK003950 D4Bwg0951e 1456251_x_at 11.08 BB132602 Bzrp 1456596_at 13.13 BB093996 6430550H21Rik 1417943_at 11.06 NM_010317 Gng4 1456746_a_at 13.05 BB334959 Cd9912 1451413_at 11.03 AB026997 Cast 1444790_at 13.04 AV048499 1810005K13Rik 1427004_at 10.98 BB311718 Fbxo2 1424246_a_at 12.98 BC003808 Tes 1418268_at 10.98 NM_013561 Htr3a 1419555_at 12.97 BC012424 Elf5 1456642_x_at 10.94 AV295650 S100a10 1439905_at 12.93 BE948639 1421483_at 10.91 NM_010712 Lhx4 1422245_a_at 12.91 U63408 Mrvi1 1430539_at 10.91 BB469133 1810057P16Rik 1417014_at 12.91 AF250139 Hspb8 1443505_at 10.87 BE651535 A630076J17Rik 1424093_x at 12.86 U89772 Cd151 1437139_at 10.84 AV344554 Glra1 1452103_at 12.77 BI456906 2600005N12Rik 1425341_at 10.81 BF467278 Kcnk3 1422870_at 12.60 NM_013553 Hoxc4 1426255_at 10.77 M20480 Nefl 1418471_at 12.54 NM_008827 Pgf 1425326_at 10.70 BI456232 Acly 1442865_at 12.50 BB361845 MGI:3580254 1454891_at 10.69 BM214378 Cds2 1435813_at 12.44 BM212838 Mypn 1427375_at 10.68 BB198308 Rg9mtd2 1438948_x_at 12.43 AV101079 Bzrp 1426926_at 10.66 AW546508 Plcg2 1429007_at 12.43 AK003377 Slc35b2 1420559_a_at 10.64 NM_013665 Shox2 1455931_at 12.42 BB460687 Chrna3 1422185_a_at 10.62 NM_029787 Cyb5r3 1436528_at 10.60 AI842353 Kazald1 1426444_at 9.49 BB233055 Rhbdl7 1417389_at 10.56 NM_016696 Gpc1 AFFX-r2-Bs- 9.47 AFFX-r2-Bs- 1445475_at 10.56 BB003750 Pak6 thr-M_s_at thr-M 1445124_at 10.54 BB193580 A930009L07Rik 1422481_at 9.47 NM_008473 Krt2-1 1418916_a_at 10.54 NM_029269 Spp2 1424976_at 9.43 BC021307 Rhov 1448883_at 10.53 NM_011175 Lgmn 1450001_a_at 9.40 NM_023649 Ush1c 1452806_at 10.51 AK005271 1500016010Rik 1460378_a_at 9.40 BC010465 Tes 1427539_a_at 10.50 BC013559 Zwint 1441481_at 9.39 AV262974 Mfap3l 1455162_at 10.50 BI147002 4922503N01Rik 1428190_at 9.37 AK005070 Slc25a1 1434976_x_at 10.49 AV216412 Eif4ebp1 1457696_at 9.36 AI835553 Rilp 1424847_at 10.46 M35131 Nefh 1439186_at 9.34 BB022463 E130319B15Rik 1450389_s_at 10.45 NM_008846 Pip5k1a 1429775_a_at 9.33 AK009736 Tm7sf1 1456524_at 10.44 AV318914 Nrg1 1424580_at 9.33 AW822833 Slc35a3 1421391_at 10.44 NM_009511 Vipr2 1437263_at 9.32 BB138441 A730089K16Rik 1449397_at 10.43 NM_134032 Hoxb2 1459888_at 9.31 AI429562 LOC545261 1417988_at 10.42 NM_009049 Resp18 1460351_at 9.24 BC021916 S100a11 1457281_at 10.41 BB224547 4930461P20Rik 1453341_a_at 9.22 AK008510 4930425N13Rik 1451680_at 10.36 BC011325 Srxn1 1425050_at 9.20 AK010892 Isoc1 1417473_a_at 10.35 NM_026494 Ppcs 1419455_at 9.18 NM_008349 Il10rb 1456187_at 10.33 BB278836 Slc7a14 1423720_a_at 9.18 BC005549 Sara1 1436035_at 10.31 AV225683 3830431G21Rik 1450444_a_at 9.16 NM_013839 Nr1h3 1424048_a_at 10.31 BC024618 Cyb5r1 1434092_at 9.14 AW121498 Nos3as 1421781_at 10.29 NM_009476 Upk2 1437670_x_at 9.13 BB113673 Cd151 1416695_at 10.28 BC002055 Bzrp 1452936_at 9.08 AV341285 Crtac1 1416750_at 10.27 NM_011014 Oprs1 1417133_at 9.06 NM_008885 Pmp22 1429348_at 10.27 AK004119 Sema3c 1423425_at 9.03 BB018522 1300012G16Rik 1448781_at 10.23 NM_008667 Nab1 1447773_x_at 9.02 BB308792 6330409D20Rik 1418403_at 10.22 NM_009616 Adam19 1449426_a_at 8.98 NM_011922 Anxa10 1441280_at 10.19 BF322939 Kcnk12 1429684_at 8.97 BG094398 5830472M02Rik 1431597_a_at 10.16 AK020731 Nrip3 1423561_at 8.94 AI838010 Nell2 1425963_at 10.16 AF419324 Cabp7 1418710_at 8.94 NM_007652 Cd59a 1416111_at 10.16 NM_009856 Cd83 1460174_at 8.92 NM_021428 Dexi 1427940_s_at 10.13 BB046347 Mycbp 1449848_at 8.91 NM_008137 Gna14 1451345_at 10.12 BG075139 Mtap 1460191_at 8.91 NM_019661 0610042I15Rik 1452449_at 10.10 AI006067 F830020C16Rik 1439006_x_at 8.89 BB093996 6430550H21Rik 1456747_x_at 10.05 BB334959 Cd99l2 1449619_s_at 8.88 AU043488 Arhgap9 1435815_at 10.03 AW541598 Ldoc1 1416592_at 8.88 AF276917 Glrx1 1427951_s_at 10.02 BG141739 Ccdc28a 1451099_at 8.86 BC011482 Mbc2 1432224_at 10.02 AK019510 4831407H17Rik 1420664_s_at 8.85 NM_011171 Procr 1432211_a_at 9.97 AK018482 Fbxo9 1460242_at 8.85 NM_010016 Daf1 1455736_at 9.97 AI326984 Mybpc2 1416168_at 8.83 NM_011340 Serpinf1 1436295_at 9.95 BB803143 Hcrtr1 1460379_at 8.82 AV307188 Hoxb4 1449079_s_at 9.90 NM_018784 St3gal6 1415919_at 8.82 NM_008721 Npdc1 1434055_at 9.83 BB048942 Galnt9 1446729_at 8.81 BB179083 Disp2 1455947_at 9.83 BB667208 9630025C22 1448722_s_at 8.81 NM_026494 Ppcs 1423966_at 9.78 BB038546 Cd99l2 1434528_at 8.79 AV256613 Aard 1419100_at 9.78 NM_009252 Serpina3n 1431160_x_at 8.78 AW543121 6030426L16Rik 1416871_at 9.77 NM_007403 Adam8 1418345_at 8.77 NM_023517 ″Tnfsf13 ; 1456229_at 9.70 BG073383 Hoxb3 Tnfsf12- 1426595_at 9.69 BB011871 Slc18a1 tnfsf13″ 1421195_at 9.69 BC020534 Cckar 1421834_at 8.76 NM_008846 Pip5k1a 1430521_s_at 9.65 AW548480 Cpne8 1425052_at 8.74 AK010892 2610034N03Rik 1430114_at 9.62 AK017323 5430420C16Rik 1430577_at 8.68 BE686390 2410007P03Rik 1457429_s_at 9.61 BB549174 Gm106 1441363_at 8.67 BQ174855 LOC546381 1459665_s_at 9.57 BB800078 Mrvil 1416435_at 8.66 NM_010736 Ltbr 1418713_at 9.57 NM_025273 Pcbd1 1416148_at 8.66 BC019120 Laptm4b 1451210_at 8.65 BC010332 Ppap2c 1415818_at 7.88 NM_013472 Anxa6 1449928_at 8.65 NM_025975 Tcte1l 1437040_at 7.85 AV280878 Etnk2 1422520_at 8.64 NM_008691 Nef3 1418107_at 7.84 NM_009326 Tcea2 1436948_a_at 8.62 BB520013 6430550H21Rik 1437648_at 7.84 BB541022 Pcyt1b 1434046_at 8.58 BG961961 AA467197 1433600_at 7.83 BB262415 1435972_at 8.57 BB148748 Cast 1426542_at 7.82 BF168366 2310067E08Rik 1460702_at 8.56 AK007514 1810015M01Rik 1441049_at 7.81 AV328356 Kcna6 1456085_x_at 8.55 AV169215 Cd151 1418686_at 7.80 NM_033541 Oas1c 1427340_at 8.50 BB274058 Rgs6 1449921_s_at 7.80 NM_009947 Cpne6 1420566_at 8.46 NM_025367 1440531_at 7.80 BB333100 Rbm11 1429246_a_at 8.45 AK013026 Anxa6 1416221_at 7.80 BI452727 Fstl1 1426319_at 8.44 AF335583 Pdgfd 1421606_a_at 7.79 NM_013873 Sult4a1 1438055_at 8.43 BB035017 Rarres1 1418572_x_at 7.79 NM_013749 Tnfrsf12a 1439807_at 8.43 BB816169 Tmem74 1452450_at 7.79 BG277213 Atp8b2 1453067_at 8.43 AU043467 2610040C18Rik 1439959_at 7.77 AW488620 Fgfl1 1424129_at 8.41 BC024891 Mfsd1 1431105_a_at 7.77 AK012413 Tmem33 1425828_at 8.40 AF357883 Nkx6-1 1445424_at 7.76 BB082380 1448429_at 8.38 NM_013755 Gyg1 1415856_at 7.75 BG064842 Emb 1456778_at 8.34 BB409477 Nrp2 1427182_s_at 7.73 BQ032363 D18Ertd653e 1430648_at 8.32 AK013326 Scn2b 1441902_x_at 7.73 BB264298 AFFX-ThrX- 8.31 AFFX-ThrX- 1441284_at 7.72 BB283819 M_at M 1451707_s_at 7.72 BC011108 Slc41a3 1452399_at 8.29 BB274058 Rgs6 1439603_at 7.72 AW125555 1458932_at 8.24 BB639093 Pex2 1443198_at 7.68 BB107751 1446838_at 8.20 BB292625 Atad1 1434494_at 7.68 BG071693 Zar1 1460377_a_at 8.18 BC005722 Tmem8 1426672_at 7.64 BB327301 Tmem16k 1423345_at 8.18 AV286991 Degs1 1420654_a_at 7.64 NM_028803 Gbe1 1448693_at 8.17 NM_024472 BC002216 1448260_at 7.64 NM_011670 Uchl1 1430999_a_at 8.15 BM932452 Scoc ″AFFX-r2- 1430778_a_at 8.15 AK020876 Nubp1 Bs-phe-5_at″ 7.63 AFFX-r2-Bs- AFFX-LysX- 8.14 AFFX-LysX-3 phe-5 3_at 1417038_at 7.63 NM_017380 1435134_at 8.12 AV369935 Aadacl1 1434665_at 7.63 AV052058 Aga 1451666_at 8.12 BI456232 Acly 1417430_at 7.62 NM_007672 Cdr2 1449961_at 8.10 NM_011286 Rph3a 1441551_at 7.60 BB196537 Mypn 1436509_at 8.09 BB000110 2410014A08Rik 1420731_a_at 7.60 NM_007792 Csrp2 1438717_a_at 8.07 BG070848 Osbpl6 1423087_a_at 7.60 BB453951 1110002E23Rik 1432319_at 8.07 AK007004 1700085C21Rik 1430780_a_at 7.60 BI739353 Pmm1 1427708_a_at 8.06 L22989 Nf2 1431086_s_at 7.60 AA389937 Pcmt1 1453523_at 8.03 AK020698 A030006P16Rik 1417780_at 7.59 BB006809 Lass4 1428400_at 8.03 BB066985 2200002K05Rik 1455783_at 7.58 AV361804 1429204_at 8.03 AK013788 2900075A18Rik 1426622_a_at 7.57 BB150720 Qpct 1429203_at 7.99 AK010725 2410076121Rik ″AFFX-r2- 1460239_at 7.97 BB807707 Tspan13 Bs-dap-5_at″ 7.56 AFFX-r2-Bs- 1453232_at 7.97 AK006582 Calr3 dap-5 1442600_at 7.97 BB456595 1443745_s_at 7.55 AV020965 Dmp1 1437107_at 7.95 AV220161 Rab6b 1449139_at 7.54 NM_025509 2310008M10Rik 1429266_at 7.95 AK020670 Tmhs 1430514_a_at 7.52 AK002762 2410026K10Rik 1452362_at 7.94 BB834440 Trim16 1440071_at 7.52 BB367260 Baiap1 1419473_a_at 7.94 NM_031161 Cck 1418173_at 7.50 NM_133730 4631426H08Rik 1444204_at 7.93 BE687976 Hoxb2 1437217_at 7.50 BM225135 Ankrd6 1429087_at 7.91 AK005245 1110054O05Rik 1425573_a_at 7.48 BE943736 Ddef1 1424050_s_at 7.91 M33760 Fgfr1 1449491_at 7.47 NM_130859 Card10 1424518_at 7.90 BC020489 ″2310016F22Rik 1421321_a_at 7.47 NM_019671 Net1 ; 1451828_a_at 7.47 AB033886 Acsl4 LOC223672″ 1420693_at 7.47 NM_010867 Myom1 1440413_at 7.46 BB800596 A830006F12Rik 1448944_at 6.96 AK011144 Nrp1 1449189_at 7.45 NM_030252 BC003266 1424178_at 6.94 BC014728 Tmem38a 1418175_at 7.44 AV290079 Vdr 1454604_s_at 6.93 BB072896 Tspan12 1417507_at 7.43 BC006732 Cyb561 1420386_at 6.93 NM_028112 Seh1l 1417969_at 7.42 NM_133765 Fbxo31 1455622_at 6.89 BB461988 Podxl2 1425329_a_at 7.42 AF332060 Cyb5r3 1418752_at 6.89 NM_007436 Aldh3a1 1450731_s_at 7.39 BG972377 Tnfrsf21 1443812_x_at 6.89 BB319311 Prkg1 1416046_a_at 7.39 BM054266 Fuca2 ″AFFX- 1422583_at 7.37 NM_023537 Rab3b DapX-5_at″ 6.88 AFFX-DapX-5 1417902_at 7.37 NM_054087 Slc19a2 1443746_x_at 6.86 AV020965 Dmp1 1426543_x_at 7.36 BF168366 2310067E08Rik 1418084_at 6.85 AK011144 Nrp1 1449983_a_at 7.35 NM_020282 Nqo2 1443664_s_at 6.85 AW123724 Cd164l2 1424560_at 7.35 U87814 Pstpip1 1451386_at 6.84 BC027279 Blvrb 1431255_at 7.34 AI324734 Calr3 1429783_at 6.83 AK009464 Pdlim5 1417539_at 7.34 NM_011895 Slc35a1 1452545_a_at 6.80 U37029 Itgb1 1424882_a_at 7.34 BC011230 2510015F01Rik 1450440_at 6.79 BE534815 Gfra1 1418206_at 7.34 NM_022324 Sdf2l1 1460409_at 6.79 AI987925 Cpt1a 1436339_at 7.32 BE627374 1810058I24Rik 1418474_at 6.79 NM_033146 1500005A01Rik 1418248_at 7.27 NM_013463 Gla 1430726_at 6.78 AV331288 Rassf8 1449012_s_at 7.25 BC027164 Fndc4 1438623_x_at 6.78 AV038578 Rbx1 1435523_s_at 7.25 BB779140 2700089E24Rik 1433796_at 6.77 BI734389 2310067E08Rik 1431946_a_at 7.24 AK013520 Apba2bp 1455085_at 6.76 BI526033 1700086L19Rik 1435575_at 7.21 AW536884 Kntc1 1427548_a_at 6.75 U53455 Clns1a 1427919_at 7.19 BC028307 Srpx2 1442015_at 6.75 BG070940 1419728_at 7.18 NM_009141 Cxcl5 1422854 _at 6.74 BB753533 Shc1 1418129_at 7.18 BG295389 Dhcr24 1416593_at 6.72 AF276917 Glrx1 1455025_at 7.14 AV103696 Paqr9 1436902_x_at 6.70 BB096368 Tmsb10 1450500_at 7.13 AW012751 Uhmk1 1459717_at 6.70 AW045505 1418478_at 7.13 NM_057173 Lmo1 1436861_at 6.68 BG069762 Il7 1434256_s_at 7.13 BM214378 Cds2 1418451_at 6.68 BB522409 Gng2 1448549_a_at 7.12 NM_007875 Dpagt1 1427196_at 6.68 BG074348 Wnk4 1455775_at 7.12 AW988196 1450138_a_at 6.67 NM_009254 Serpinb6a 1429712_at 7.12 AK005093 Etohi1 1448772_at 6.65 BG868960 Ube2a 1445564_at 7.11 BE688513 1418452_at 6.65 BB522409 Gng2 1421127_at 7.10 NM_025339 Tmem42 1444178_at 6.64 BB609792 LOC433237 1430631_at 7.10 AK016942 Ppm1f 1455427_at 6.64 AV269710 Angpt4 1416914_s_at 7.10 NM_023166 Mtvr2 1423954_at 6.64 K02782 C3 1429300_at 7.09 AK010867 Ankrd9 1423280_at 6.64 BM115022 Stmn2 1425440_x_at 7.09 BC011108 Slc41a3 1460375_at 6.62 BC019418 0610038D11Rik 1422158_at 7.08 NM_022319 Clstn2 1419398_a_at 6.61 NM_007874 Dp1 1418371_at 7.07 AY029255 6720463E02Rik 1419383_at 6.61 NM_009115 1454893_at 7.06 BB765852 1110013L07Rik 1454227_at 6.61 AK015389 Htatip2 1418187_at 7.04 AF146523 Ramp2 1438007_at 6.60 BB432758 AI851790 1417807_at 7.04 NM_027356 2700038NO3Rik 1419518_at 6.59 NM_017379 Tuba8 1424486_a_at 7.03 BB284199 Txnrd1 1431446_at 6.57 AK002366 0610009B14Rik 1424109_a_at 7.02 BC024663 Glo1 1428139_at 6.56 AK015988 4930538D17Rik 1452807_s_at 7.02 AK005271 1500016O10Rik 1436150_at 6.54 BB391675 2310028H24Rik 1433787_at 7.01 AI841091 B230343H07Rik 1450486_a_at 6.54 X91813 Oprl1 1433793_s_at 7.01 AW491344 Nrip2 1416673_at 6.53 NM_019517 Bace2 1445437_at 7.00 BE948587 2310015A05Rik 1419550_a_at 6.52 BG919998 Stk39 1456857_at 6.99 BB782615 1437043_a_at 6.52 AV012400 1110012M11Rik 1451427_a_at 6.99 BC024610 Egfl7 1450787_at 6.48 NM_016691 Clcn5 1418890_a_at 6.98 BB349707 Rab3d 1420772_a_at 6.47 NM_010286 Tsc22d3 1440248_at 6.97 BB238478 Casc4 ″AFFX- 1460466_at 6.97 BB824055 1700047117Rik PheX-M_at″ 6.46 AFFX-PheX- 1439407_x at 6.96 AV212626 Tagln2 M 1441281_s_at 6.45 AU024536 Ninj1 1418104_at 6.09 NM_020610 Nrip3 1446218_at 6.42 BB306329 Ppfia2 1421034_a_at 6.09 NM_010557 Il4ra 1449433_at 6.42 NM_033321 P2rx5 1431241_at 6.08 AA692147 Chchd3 1422597_at 6.41 NM_008609 Mmp15 1429522_at 6.07 BB796723 Ankrd42 1417290_at 6.41 NM_029796 Lrg1 1429753_at 6.07 AK004130 Nxph4 1444291_at 6.41 BB184872 4930506D23Rik 1427773_a_at 6.07 L40934 Rabac1 1441456_at 6.40 BB335489 Mmp24 1440308_at 6.06 AV347071 0610011D08Rik 1448300_at 6.40 NM_025569 Mgst3 1424732_s_at 6.06 BC020157 3110005G23Rik 1452825_at 6.40 AK005155 5330410G16Rik 1455197_at 6.05 BE852181 Rnd1 1427956_at 6.40 BG068910 Pcgf1 1431684_at 6.05 AK016622 4933402J24Rik 1417399_at 6.38 NM_019521 Gas6 1424773_at 6.03 BC020119 1110012M11Rik 1438635_x_at 6.38 BB258476 B930041F14Rik 1417040_a_at 6.03 NM_016778 Bok 1449460_at 6.38 AF403041 Asb13 1418934_at 6.02 NM_011839 Mab21l2 1426858_at 6.37 BB253137 Inhbb 1425053_at 6.02 AK010892 Isoc1 1428495_at 6.35 BF300229 2410003K15Rik 1423321_at 6.01 BI078799 Myadm 1423951_at 6.35 BC024620 Tm2d3 1429756_at 6.01 AK016481 4931428F04Rik 141599_at 6.35 NM_009270 Sqle ″AFFX-r2- 1451426_at 6.34 AF316999 D11Lgp2e Bs-lys-M_at″ 6.01 AFFX-r2-Bs- 1417022_at 6.34 NM_007515 Slc7a3 lys-M 1422620_s_at 6.33 NM_008903 Ppap2a 1451428_x_at 6.00 BC024610 Egfl7 1423840_at 6.33 BC026206 D11Ertd99e 1460059_at 6.00 BB272732 Upp2 1429840_at 6.33 AW491495 4933439G12Rik 1420712_a_at 6.00 NM_008281 Hpn 1424356_a_at 6.32 BC024445 Metrnl 1436408_at 5.99 BE946298 1452244_at 6.31 AK018128 6330406I15Rik 1422645_at 5.98 AJ306425 Hfe 1442011_at 6.31 BB177150 A230065H16Rik 1423948_at 5.98 BC016230 Bag2 1416710_at 6.30 NM_026239 Tmem35 1429830_a_at 5.97 AK005507 Cd59a 1416410_at 6.30 NM_008776 Pafah1b3 1435332_at 5.96 BQ175864 Htr7 1437501_at 6.30 BB125794 AF529169 1421015_s_at 5.96 NM_021498 Pole3 1431359_a_at 6.29 AK003526 1110007C09Rik ″AFFX- 1450738_at 6.29 NM_016705 Kif21a LysX-M_at″ 5.94 AFFX-LysX- 1433462_a_at 6.29 BB208212 Pi4k2a M 1454811_a_at 6.28 AV026664 Tde2 1451501_a_at 5.94 BC024375 Ghr 1424426_at 6.28 BG075139 Mtap 1454219_at 5.94 AK013295 Dnajc2 1444687_at 6.27 BB804635 C1ql2 1459669_at 5.94 AW913782 D930010J01Rik 1458438_at 6.27 AW554226 4933415L06Rik 1457076_at 5.93 BQ175967 Gm693 1426376_at 6.25 BI249075 Dp1 1448477_at 5.93 NM_021528 Chst12 1430520_at 6.25 AW548480 Cpne8 1436127_at 5.93 AI854101 Crhbp 1427202_at 6.24 AV002340 4833442J19Rik 1428823_at 5.93 AK009957 1416979_at 6.24 NM_025624 2510048O06Rik 1449473_s_at 5.91 NM_011611 Cd40 1433835_at 6.23 BE825122 Ppp3cb 1436718_at 5.91 BB274960 Nxph1 1431226_a_at 6.21 AK013203 Fndc4 ″AFFX- 1458413_at 6.20 BB357976 Fbxw8 PheX-3_at″ 5.91 AFFX-PheX-3 1418359_at 6.20 NM_024479 Wbscr27 1450040_at 5.90 M93954 Timp2 1452608_at 6.20 BB046347 Mycbp 1436416_x_at 5.88 BB609429 Fxc1 1422637_at 6.20 NM_018750 Rassf5 1458395_at 5.84 BB811756 B930054O08 1432271_a_at 6.19 AK012764 Dcun1d5 1426220_at 5.84 BC019457 4930471M23Rik ″AFFX-r2- 1449347_a_at 5.83 NM_021365 Xlr4b ; Xlr4a Bs-phe-M_at″ 6.16 AFFX-r2-Bs- 1427246_at 5.83 BB702366 Magi1 phe-M 1448699_at 5.82 NM_025317 Mrpl54 1417458_s_at 6.16 NM_025415 Cks2 1448852_at 5.82 NM_009060 Rgn 1417026_at 6.15 BC024693 Pfdn1 1453605_s_at 5.82 AK007017 1810060J02Rik 1417979_at 6.15 AF291655 Tnmd 1449283_a_at 5.82 BC021640 Mapk12 1417203_at 6.15 BC010592 Ethe1 1437292_at 5.81 BB431052 A330019N05Rik 1423101_at 6.14 BB279185 Paqr4 1432349_a_at 5.81 AK004279 Sync 1430098_at 6.14 AK018150 6330409D20Rik 1460150_at 5.81 BE979371 Sh2d3c 1429273_at 6.11 AK014221 Bmper 1438568_at 5.81 BB373312 Mrgpre 1417149_at 5.80 NM_011031 P4ha2 1428357_at 5.59 AK011462 2610019F03Rik 1451683_x_at 5.80 M34962 H2-D1 1419118_at 5.57 NM_021387 2900093B09Rik 1455346_at 5.80 BB477214 Masp1 1428804_at 5.57 AK017269 Mfap3l 1450661_x_at 5.79 NM_008688 Nfic 1429110_a_at 5.56 AW701004 2810405F18Rik 1444009_at 5.78 AV217868 Rassf4 1416022_at 5.55 BC002008 Fabp5 1437637_at 5.77 BE852433 Phtf2 1422965_at 5.55 NM_009642 Agtrap 1458299_s_at 5.76 BB820441 Nfkbie 1443811_at 5.55 BB319311 Prkg1 1454771_at 5.76 AW060701 1430332_a_at 5.55 AK002832 Gusb 1454800_at 5.76 BF319573 MGI:2674071 1453281_at 5.54 BB700084 Pik3cd 1424767_at 5.76 AB019618 Cdh22 1448987_at 5.54 BB728073 Acadl 1459552_at 5.75 BB404047 1439221_s_at 5.54 BB220422 Cd40 1453607_at 5.75 AV280494 Mfap3l 1435404_at 5.54 BB023743 Disp2 1418344_at 5.75 NM_021793 Tmem8 1453461_at 5.53 AK002949 Fxc1 1423211_at 5.75 AK004120 Nola3 1427373_at 5.53 BC010598 Amigo1 1416994_at 5.74 NM_133795 Ttc1 1434049_at 5.53 BB547934 Entpd3 1428377_at 5.74 AK018115 Btbd11 1452717_at 5.53 BM230959 Slc25a24 1419399_at 5.73 AW553649 Mttp 1428663_at 5.53 AK017223 5133401H06Rik 1416028_a_at 5.73 NM_008258 Hn1 1423158_at 5.52 AK008566 Gnpnat1 1427329_a_at 5.73 AI326478 Igh-6 1423686_a_at 5.52 BC016234 1110020C13Rik 1424278_a_at 5.72 BC004702 Birc5 1437462_x_at 5.52 BB484002 1454613_at 5.72 AV162270 9430041P20Rik 1424699_at 5.51 BC006583 4921511K06Rik 1438378_at 5.72 AV208934 1700013G23Rik 1457124_at 5.50 AV328224 1429743_at 5.72 BG068031 6720468P15Rik 1426298_at 5.50 AF295369 Irx2 1451690_a_at 5.71 BC024948 Pvrl4 1420554_a_at 5.49 NM_133223 Rac3 1427937_at 5.70 BB410125 ″2610030H06Ri 1433667_at 5.48 BB187947 Lgi3 k ; 1430111_a_at 5.48 AK012821 Bcat1 LOC434793 ; 1416637_at 5.46 NM_009207 Slc4a2 LOC545620″ 1452770_at 5.46 AK003237 Vkorc1 1452579_at 5.70 AK009021 Nifun 1439152_at 5.46 BE985292 BC052066 1417457_at 5.69 NM_025415 Cks2 1437936_at 5.45 AI428240 6330534C20Rik 1423552_at 5.69 BF658789 Leprotl1 1428379_at 5.45 BQ180367 Slc17a6 1416749_at 5.69 NM_019564 Htra1 1425780 a at 5.45 BC024352 0610041E09Rik 1455378_at 5.68 BI735497 BC057371 1416953 at 5.45 NM_010217 Ctgf 1432287_a_at 5.67 AK016927 Sntg1 1448592_at 5.45 NM_019922 Crtap 1436869_at 5.67 AV304616 Shh 1451817_at 5.44 BC011214 Suv420h1 1423653_at 5.67 BC025618 Atp1a1 1418482_at 5.44 NM_019720 Cyb561d2 1424842_a_at 5.67 BC025502 Arhgap24 1438388_at 5.44 BB078029 1436124_at 5.66 BE996519 Pcyt1b 1420113_s_at 5.43 AA409325 2410022L05Rik 1416230_at 5.66 NM_019437 Rfk 1420548_a_at 5.43 NM_023197 2310008H09Rik 1457232_at 5.66 BE946365 Fbxl21 1423286_at 5.41 AA016422 Cbln1 1428988_at 5.65 AK006128 Abcc3 1455793_at 5.41 BB766438 BC035537 1450010_at 5.65 AK012103 Hsd17b12 1435244_at 5.40 BF535947 Vav2 1449078_at 5.65 NM_018784 St3gal6 1453715_at 5.39 AK015921 Sv2c 1453752_at 5.65 BF453369 Rpl17 1446260_at 5.39 BB282393 Six6os1 1455147_at 5.64 BB177828 1421863_at 5.38 AK018783 Vamp1 1427481_a_at 5.64 BC027114 Atp1a3 1419740_at 5.37 NM_008806 Pde6b 1426918_at 5.64 BM120341 Itgb1 1428734_at 5.37 AK018169 3200002M19Rik 1435551_at 5.63 BG066491 FHOS2 1427181_at 5.37 BQ032363 D18Ertd653e 1425351_at 5.62 BC011325 Srxn1 1435109_at 5.37 AW123256 ″0710007G10Ri 1455812_x_at 5.62 BB530515 Slitl2 k ; 1416781_at 5.61 NM_138602 Praf2 3010001K23 1447975_a_at 5.60 AI429562 LOC545261 Rik″ 1459927_at 5.60 AA867818 4833445I07Rik 1449267_at 5.36 BC006876 3110023E09Rik 1449471_at 5.60 NM_021452 Kcnmb4 1437943_s_at 5.36 AA764430 Mea1 1418888_a_at 5.60 NM_013759 Sepx1 1448140_at 5.36 NM_134141 Ciapin1 1456231_at 5.59 BB315153 Pla2g3 1449021_at 5.36 NM_026308 Rpp21 1429212_a_at 5.36 AK005758 Lrrc51 1419254_at 5.09 BG076333 Mthfd2 1422717_at 5.35 AW554436 Acp1 1424528_at 5.09 BC023116 Cgref1 1456663_x_at 5.35 BB718785 Tm2d2 1423608_at 5.09 BI966443 Itm2a 1448472_at 5.35 AF087680 Vars2 1422559_at 5.09 AY039837 Ube2n 1438746_at 5.35 BB253959 A530058N18Rik 1435017_at 5.09 AV000569 Mel13 1425155_x_at 5.35 M21149 Csf1 1418736_at 5.08 BC003835 B3galt3 1417240_at 5.34 NM_011777 Zyx 1436250_at 5.08 BM946369 5430405G05Rik 1453558_at 5.33 AK015697 4930504H06Rik 1447683_x_at 5.08 AV118676 Mettl1 1422952_at 5.32 NM_023893 MGI:2388100 1449850_at 5.08 NM_022723 Scube1 1437950_at 5.32 BB818275 BC035537 1424403_a_at 5.07 AW494299 D5Bwg0860e 1430219_at 5.32 AK017861 Fts 1432181_s_at 5.07 AK013765 Ecgf1 1420614_at 5.31 NM_025975 Tcte1l 1429602_at 5.06 AK009888 Cd164l2 1436978_at 5.30 AV273409 Wnt9a 1450506_a_at 5.06 NM_026531 Isg20l1 1416366_at 5.30 NM_024220 Ndufc2 1450925_a_at 5.06 BB836796 Rps27l 1460030_at 5.29 BB355811 Hecw1 1460483_at 5.05 AV115271 2610034E01Rik 1436433_at 5.28 AV264828 BC049762 1430040_at 5.05 AK006867 Hspa12a 1440772_x_at 5.28 BB257157 9530077C05Rik 1460628_at 5.05 AV245208 Nme3 1425784_a_at 5.28 D78264 Olfm1 1442466_a_at 5.04 BG071019 B430315C20Rik 1451409_at 5.27 BC025858 2210021J22Rik 1436377_at 5.04 BI410102 AI428855 1436800_at 5.27 AV376318 Fstl5 1429274_at 5.04 AK009282 2310010M24Rik 1416940_at 5.27 NM_134084 Ppif 1437055_x_at 5.04 BB143944 1200003O06Rik 1451493_at 5.27 BC026372 Ndfip1 1417832_at 5.03 BB156359 Smc1l1 1451395_at 5.26 BC021492 D5Bwg0834e 1435130_at 5.03 AI131918 LOC383103 1426254_at 5.26 AF353993 Tm2d1 1418746_at 5.03 NM_019999 MGI:1930773 1436517_at 5.26 BI416101 H1fx 1460674_at 5.03 BC022922 Paqr7 1453304_s_at 5.25 BM245572 Ly6e 1454006_a_at 5.03 AK016964 Ubxd6 1423034_at 5.25 AK004219 Txnl5 1429088_at 5.03 AK007400 MGI:1925139 1432415_at 5.25 AK014050 Rab3c 1430889_a_at 5.01 AK002335 Tpmt 1426621_a_at 5.25 BB560759 Ppp2r2b 1430053_a_at 5.00 AK019142 2810409H07Rik 1432591_at 5.25 BB635017 Pappa 1447728_x_at 5.00 BB718260 Hspa9a 1416245_at 5.24 NM_025338 Aurkaip1 ″AFFX-r2- 1434980_at 5.24 AV230647 Pik3r5 Bs-phe-3_at″ 5.00 AFFX-r2-Bs- 1452923_at 5.24 AK007894 1810058I14Rik phe-3 1419445_s_at 5.23 NM_009119 Sap18 1424579_at 5.00 AW822833 Slc35a3 1430869_a_at 5.23 BF460630 Habp4 1449256_a_at 4.99 BC010722 Rab11a 1430866_at 5.23 BB432681 4921537D05Rik 1423423_at 4.99 BF319868 Pdia3 1442143_at 5.22 AI463477 Tmem16d 1434549_at 4.99 BI083615 Rab11a 1417619_at 5.22 BE368753 Gadd45gip1 1449517_at 4.99 BB119571 Qpctl 1431694_a_at 5.21 AK003595 Ctnnbip1 1422034_a_at 4.99 NM_023128 Palm 1417627_a_at 5.21 NM_010717 Limk1 ″AFFX-r2- 1435297_at 5.21 BI737842 Gja9 Bs-lys-3_at″ 4.99 AFFX-r2-Bs- 1428753_a_at 5.21 AK019346 Dgcr6 1ys-3 1452427_s_at 5.19 BM950003 AW742319 1438251_x_at 4.98 BB559067 Htra1 1442542_at 5.18 BB363812 Eya4 1454572_at 4.98 AK013093 2810414N06Rik 1425090_s_at 5.16 BC024837 Kcnc4 1449107_at 4.97 NM_027722 Nudt4 1415857_at 5.15 BG064842 Emb 1416167_at 4.97 NM_016764 Prdx4 1430777_a_at 5.14 AK014644 Golph3 1428650_at 4.97 AK003780 Tns1 1451128_s_at 5.14 BC003427 Kif22 1458997_at 4.97 BB183491 A230106M20Ri 1431507_a_at 5.14 AK008254 Synj2bp k 1431510_s_at 5.12 AK008567 2010110K16Rik 1426528_at 4.97 BQ176723 Nrp2 1453358_s_at 5.11 BB238321 ″5830467E07Ri 1438957_x_at 4.96 BB027654 Cds2 k ; 1436512_at 4.96 BI964400 Arl7 C730024G19 1449083_at 4.96 AA067702 1810060J02Rik Rik″ 1436094_at 4.95 BF458396 1426400_a_at 5.11 BI159030 Capns1 1457558_at 4.95 AV325555 A330050F15Rik 1437249_at 5.10 BG075562 Scap1 1417916_a_at 4.95 NM_019502 Fxc1 ; 8030491N06Rik 1459858_x_at 4.79 AV354465 Crtac1 1430134_a_at 4.94 AK008774 Yars2 1456233_at 4.78 BB080923 1428447_at 4.93 AK017734 Tmem14a 1435650_at 4.78 BB082407 Hapln4 1417219_s_at 4.93 NM_025284 Tmsb10 1418184_at 4.78 NM_025639 2610019I03Rik 1418281_at 4.93 NM_011234 Rad51 1436671_at 4.77 BB151200 A030011M19 1423035_s_at 4.93 AK004219 Txnl5 1420124_s_at 4.77 C85065 Tcta 1434362_at 4.92 BM249454 AW550831 1455825_s_at 4.77 BB131619 Lnx1 1435429_x_at 4.92 AV111399 Rps271 1428699_at 4.77 AW744143 Sms 1451931_x_at 4.92 M69068 H2-D1 1419124_at 4.77 NM_133829 2210010L05Rik 1457230_at 4.92 BB458901 Gnpda2 1437360_at 4.76 BB053591 Pcdh19 1436105_at 4.91 BG068839 2310015A05Rik 1451643_a_at 4.76 BC007147 Rab4b 1420873_at 4.91 BI662615 Ptk9 1434826_at 4.76 AW124912 AI256775 1451609_at 4.91 BC024685 Tspan33 1429520_a_at 4.75 BE573407 Phca 1424334_at 4.90 BC010346 Tspan17 1436341_at 4.75 BM125569 F830020C16Rik 1423082_at 4.90 AW545361 Derl1 1426187_a_at 4.75 AF465243 Hax1 1418795_at 4.90 BB487604 Cds2 1416849_at 4.75 NM_134007 D10Ertd214e 1427894_at 4.90 AK012169 Slitl2 1440815_x_at 4.74 BB099075 LOC545039 1451353_at 4.89 AV378394 Tm6sf1 1416503_at 4.74 NM_016753 Lxn 1438928_x_at 4.88 BB252065 Ninj1 1417593_at 4.74 NM_019742 Tusc2 1448887_x_at 4.88 NM_019502 Fxc1 1439963_x_at 4.73 BB660640 1700129I04Rik 1453317_a_at 4.87 AK014353 Khdrbs3 1458396_at 4.73 BB452660 Ssrp1 1435755_at 4.87 BB609468 1110001A16Rik 1429916_at 4.73 AK021129 9530077C05Rik 1418927_a_at 4.87 NM_019986 Habp4 1450868_at 4.73 BF468249 D8Ertd354e 1425118_at 4.87 BC026502 Spire2 1423170_at 4.73 AV213552 Taf7 1428605_at 4.86 AI227096 1810023B24Rik 1427230_at 4.73 BB322737 B930041F14Rik 1429984_at 4.86 AK017666 5730455013Rik 1448619_at 4.73 NM_007856 Dhcr7 1435750_at 4.85 BB610454 Gchfr 1417541_at 4.73 NM_008234 Hells 1417959_at 4.85 NM_026131 Pdlim7 1430574_at 4.72 AK010426 Cdkn3 1415681_at 4.85 NM_053164 Mrpl43 1415947_at 4.72 BC027426 Creg1 1429600_at 4.85 AK003824 1110019K23Rik 1426541_a_at 4.71 BF168366 2310067E08Rik 1442379_at 4.85 BB020678 MGC99845 1416965_at 4.71 AF181560 Pcsk1n 1434342_at 4.84 BB316114 1418743_a_at 4.71 NM_021344 MGI:1930803 1452272_a_at 4.84 BI901126 Gfer 1419056_at 4.71 AF038538 Rtn2 1426416_a_at 4.84 AV216410 Yipf4 1460639_a_at 4.71 NM_009720 Atox1 1431611_a_at 4.84 AK013775 Igsf4a 1424496_at 4.71 BC026742 5133401N09Rik 1438210_at 4.84 BB126999 Gpr149 1460711_at 4.70 BG071611 4930461P20Rik 1427921_s_at 4.83 AW045976 2310061C15Rik 1452976_a_at 4.70 AK004710 Slc9a3r2 1444908_at 4.83 BM228113 Habp4 1433950_at 4.70 BB769866 Igsf21 1417103_at 4.83 NM_010027 Ddt 1423759_a_at 4.70 BC020098 Tmco1 1428157_at 4.83 AV021455 Gng2 1430274_a_at 4.70 AK018331 Stard3nl 1415910_s_at 4.82 NM_134141 Ciapin1 1416696_at 4.70 NM_080837 D17Wsu104e 1416379_at 4.81 NM_019482 Panx1 1427943_at 4.70 BI730288 Acyp2 1424441_at 4.81 BC023114 Slc27a4 1424197_s_at 4.69 BC016538 Fance 1449536_at 4.80 NM_032397 Kcnn1 1448950_at 4.69 NM_008362 Il1r1 1457052_at 4.80 BG064867 AW536275 1447541_s_at 4.69 AV210813 Itgae 1449448_at 4.80 BC019367 Med9 1433635_at 4.68 BG073188 Wdr18 1429213_at 4.80 AK009547 2310030N02Rik 1448700_at 4.68 NM_008059 G0s2 1438713_at 4.79 BB391868 Rassf8 1450397_at 4.79 BB731480 Mtap1b

TABLE 20 Top 1,000 cerebellar Purkinje (Pcp2) cell translated mRNAs enriched when compared to a reference sample ProbeSymbol = Affymetrix probe identification corresponding to gene symbol; FEN = fold enrichment when compared to reference sample; Genbank = Genbank identification; GeneSymbol = official gene symbol. ProbeSymbol FEN Genbank GeneSymbol ProbeSymbol FEN Genbank GeneSymbol 1438405_at 520.4 BB791906 Fgf7 1446771_at 44.34 BB047533 1450079_at 441.1 AK012873 Nrk 1457011_at 44.01 BB797251 1440436_at 353.6 BQ176471 A730030A06 1424443_at 43.15 AV378394 Tm6sf1 1449240_at 333.9 BC026822 MGI:1333876 1417552_at 42.43 NM_007986 Fap 1427624_s_at 304.2 AJ249492 Il22 ; Iltifb 1447405_at 42.03 BB479096 1420994_at 301 BM214359 B3gnt5 1442751_at 41.29 AI587721 D430034N21 1432515_at 298.4 AK010774 2410124H12Rik 1449102_at 40.59 U71189 Ebf2 1419084_a_at 289.1 NM_008790 Pcp2 1437836_x_at 38.92 BB357560 0610011L14Rik 1436410_at 254.9 BB346846 AI449023 1453127_at 38.89 AK009235 Ppm1j 1418095_at 223.9 NM_025357 Smpx 1433579_at 38.28 BG068678 Tmem30b 1427291_at 180.5 AV275615 Sycp1 1437254_at 38.26 BB315525 6230425F05Rik 1435750_at 168.7 BB610454 Gchfr 1416892_s_at 37.97 BC021353 3110001A13Rik 1424958_at 168.2 BC010773 Car8 1459589_at 37.6 C85932 Cfyl1 1424859_at 155.8 BC005773 Homer3 1438708_x_at 37.14 BI465579 Ywhab 1457904_at 144.2 BB125515 1423452_at 37.03 AV173139 Stk17b 1455970_at 134.4 BE370618 1445913_at 36.44 AV251751 A730090H04Rik 1458341_x_at 130.3 BB397841 1433553_at 36.26 BB131106 Garnl3 1443699_at 119.9 BE957383 Ptprk 1427760_s_at 35.5 X75557 Plf ; Plf2 ; 1419518_at 109.9 NM_017379 Tuba8 Mrpplf3 1458284_at 108.9 BM195499 Ptbp1 ; 1419061_at 35.41 D89821 Rhod LOC236294 1455431_at 33.78 AV371434 Slc5a1 1450428_at 106.6 AV335209 Lhx1 1458434_at 33.56 BB245484 A730009L09Rik 1457523_at 98.38 BB125017 9530056K15Rik 1456798_at 32.78 BB317517 9330118A15Rik 1448509_at 96.98 BC021353 3110001A13Rik 1455758_at 32.74 BM215011 1439232_at 93.96 BB781460 1425469_a_at 32.47 BC003855 1437209_at 89.42 AV324566 6230425F05Rik 1454884_at 32.03 BG073185 Btbd4 1435275_at 84.82 AV013496 Cox6b2 1430310_at 32.02 BB535262 Tspan11 1438340_at 80.48 BB277600 A930006D11 1457337_at 31.88 AV028487 1447742_at 79.1 BB216290 Laptm5 1453223_s_at 31.6 AK010743 Dppa2 1429346_a_at 78.36 BB733638 4933440J22Rik 1421951_at 31.31 AV335209 Lhx1 1419057_at 77.86 AF208031 Slc5a1 1431197_at 31.19 BB641782 Arl6ip2 1450078_at 77.37 AK012873 Nrk 1443824_s_at 31.1 BB193643 Car7 1425179_at 75.87 AF237702 Shmt1 1445576_at 30.75 AI413817 4930526H21Rik 1455512_at 75.01 AI852151 1417597_at 30.24 NM_007642 Cd28 1418173_at 69.35 NM_133730 4631426H08Rik 1429719_at 30.19 AK009204 Foxp4 1441776_at 68.9 BB732603 Tspan11 1440727_at 30.1 BF472124 1434973_at 68.36 BE650380 Car7 1424046_at 29.81 AF002823 Bub1 1440944_at 66.97 BF463236 LOC544718 1447439_at 29.67 AI848394 1700023E05Rik 1450997_at 66.54 AV173139 Stk17b 1419326_at 29.63 NM_026188 1700028P14Rik 1460118_at 63.38 BF455409 1427676_a_at 29.62 X66118 Grik1 1418494_at 61.18 U71189 Ebf2 1420348_at 29.14 NM_008499 Lhx5 1436035_at 57.75 AV225683 3830431G21Rik 1438773_at 28.87 BB817972 Steap2 1427707_a_at 57.14 BC004585 Sil 1416893_at 28.74 BC021353 3110001A13Rik 1434588_x_at 52.98 AI181686 Tbca 1439745_at 28.58 AV338037 Cacng7 1452484_at 52.2 AF291660 Car7 1448738_at 28.2 BB246032 Calb1 1459995_at 51.58 AV047656 1700015G11Rik 1460482_at 27.39 AW123948 3110047P20Rik 1457558_at 51.52 AV325555 A330050F15Rik 1437025_at 27.27 AV313615 Cd28 1437580_s_at 51.44 C77054 Nek2 1455607_at 26.98 BG072958 Rspo3 1423366_at 50.63 BE133651 Scd3 1417299_at 26.87 NM_010892 Nek2 1422243_at 49.69 NM_008008 Fgf7 1458667_at 26.77 AV266695 4930519N13Rik 1417843_s_at 47.32 NM_133191 Eps812 1443999_at 26.77 BB203873 Nek2 1441166_at 46.97 AW123460 A330050F15Rik 1421129_a_at 26.42 NM_016745 Atp2a3 1433639_at 46.62 AW548096 5730593F17Rik 1456834_at 26.03 BB277828 Ibrdc2 1422481_at 46.07 NM_008473 Krt2-1 1452404_at 25.64 BB321846 Phactr2 1418933_at 45.46 NM_009200 Slc1a6 1453904_at 25.52 AK015926 4930528G09Rik 1428536_at 44.48 AK015001 Kcng4 1441785_at 25.37 AW120650 1423978_at 25.35 BC025837 Sbk1 1422752_at 18.77 BG070121 Polr3k 1441236_at 25.32 AV373598 Slc9a3 1433582_at 18.76 AV309085 1190002N15Rik 1452986_at 25.31 AK002584 Hgd 1455952_at 18.69 AI503324 Adprhl1 1446612_at 25.06 BB169371 9330118A15Rik 1449874_at 18.66 NM_016923 Ly96 1426015_s_at 24.98 AF302653 Asph 1435959_at 18.4 BM246535 Arhgap15 1416794_at 24.9 NM_019717 Arl6ip2 1436326_at 18.27 BB306272 Rora 1428349_s_at 24.75 AK014058 Ebf3 1437880_at 18.02 AV273001 Lbxcor1 1429262_at 24.27 AK005472 Rassf6 1433892_at 18.02 BM208112 Spag5 1438558_x_at 24.07 AV009267 Foxq1 1418237_s_at 17.9 NM_009929 Col18a1 1426047_a_at 23.71 AF129509 Ptprr 1421392_a_at 17.78 NM_007464 Birc3 1440815_x_at 23.69 BB099075 LOC545039 1452889_at 17.75 AK009207 2310007H09Rik 1446894_at 23.56 BB117494 Arnt2 1437444_at 17.74 BB479976 Pcdhgc3 1442209_at 23.42 BB251707 2400009B08Rik 1448355_at 17.71 NM_019429 Prss16 1456729_x_at 23.29 BB218661 Rtel1 1426955_at 17.58 D17546 Col18a1 1418957_at 23.25 NM_016853 Stac 1420334_at 17.54 NM_134251 Slc12a8 1429712_at 23.19 AK005093 Etohi1 1418733_at 17.53 NM_011658 Twist1 1421309_at 22.81 NM_008598 Mgmt 1451931_x_at 17.43 M69068 H2-D1 1447828_x at 22.69 BB226589 2310005P05Rik 1419985_s_at 17.41 C87579 D11Ertd461e 1439181_at 22.67 AV020760 BC043301 1436716_at 17.3 BM941586 Ppp1r14b 1443939_at 22.58 BF021397 LOC230628 1445173_at 17.28 BF472675 AK129341 1443591_at 22.42 BB827848 Dlx4 1452704_at 17.28 AK004786 1200015F23Rik 1420052_x_at 22.4 C81484 Psmb1 1422529_s_at 17.26 NM_009814 Casq2 1433605_at 22.29 BB307136 Inpp5a 1455977_x_at 17.2 AV053098 Klk6 ; Klk5 1450956_at 21.88 BE133651 Scd3 1437101_at 17.18 AV271979 Lats2 1436627_at 21.84 BB368203 D17Ertd663e 1439146_s_at 16.94 AA867167 Lck 1440688_at 21.83 BM244666 Arhgap26 1439729_at 16.77 BB463084 A930038B10Rik 1439834_at 21.73 AA261399 2400009B08Rik 1439377_x_at 16.68 BB041150 Cdc20 1454171_x_at 21.52 AK020608 9530053H05Rik 1448459_at 16.6 NM_027398 Kcnip1 1433588_at 21.11 BM238232 D6Wsu116e 1422537_a_at 16.52 NM_010496 Id2 1460666_a_at 20.9 NM_010096 Ebf3 1429166_s_at 16.42 AW494443 Clmn 1437250_at 20.87 AV298358 MGI:2151839 ″AFFX- 1416698_a_at 20.85 NM_016904 Cks1b DapX-3_at″ 16.41 AFFX-DapX- 1447178_at 20.83 BB173057 3 1459801_at 20.67 BB189298 B3galt5 1435487_at 16.38 BB049642 Grid2 1448117_at 20.66 BB815530 Kitl 1417088_at 16.37 AW493043 Zfp346 ″AFFX-r2- 1428977_at 16.23 AK005217 Chst8 Bs-dap_3 at″ 20.63 AFFX-r2-Bs- 1439266_a_at 16.18 AV260647 Polr3k dap-3 1423790_at 16.14 BC024876 Dap 1451683_x at 20.56 M34962 H2-D1 1455101_at 16.12 BE631955 1424904_at 20.35 BC019143 1300010F03Rik 1459660_at 16.05 BE944772 1417178_at 20.32 NM_016867 Gipc2 1457441_at 16.04 AI385540 Ebf1 1420993_at 20.27 BM214359 B3gnt5 1431446_at 16 AK002366 0610009B14Rik 1446071_at 20.07 BB529332 Steap2 1428196_a_at 15.96 AK004786 1200015F23Rik 1454653_at 19.99 BB274531 A730016F12Rik 1415947_at 15.95 BC027426 Creg1 1454079_at 19.88 AK016591 4933400F03Rik 1450389_s_at 15.78 NM_008846 Pip5k1a 1451112_s_at 19.86 BC024876 Dap 1420453_at 15.73 NM_009967 Crygs 1419473_a_at 19.72 NM_031161 Cck 1418709_at 15.68 AF037370 Cox7a1 1425198_at 19.7 BG076152 Ptpn2 1424826_s_at 15.5 BC024131 Mtss1 1441836_x_at 19.66 AV045004 1700006H03Rik 1454713_s_at 15.43 BG072171 Hdc 1447004_at 19.26 BB128185 1420583_a_at 15.35 NM_013646 Rora 1433893_s_at 19.24 BM208112 Spag5 1451707_s_at 15.31 BC011108 Slc41a3 1415855_at 18.97 BB815530 Kitl 1434201_at 15.21 AV144145 Chrdl1 1426738_at 18.96 BC014860 Dgkz 1417164_at 15.19 NM_022019 Dusp10 1420664_s_at 18.94 NM_011171 Procr 1421446_at 15.12 NM_011102 Prkcc 1417577_at 18.9 NM_019510 Trpc3 1444790_at 15.08 AV048499 1810005K13Rik 1443560_at 18.77 BB283805 Pofut2 1420522_at 15.06 NM_026202 2610529H08Rik 1450276_a_at 15.03 NM_009132 Scin 1434006_at 12.27 BQ030992 BC051227 1427178_at 14.89 BB667469 Tmc4 1436948_a_at 12.14 BB520013 6430550H21Rik 1444582_at 14.68 AV032530 4833420K19Rik 1439254_at 12 BE981392 Akap13 1435226_at 14.46 BG064140 Ibrdc3 1452751_at 11.99 AK014058 Ebf3 1423365_at 14.4 AW494038 Cacna1g 1424037_at 11.97 BC027291 Itpka 1423652_at 14.39 AV209097 Hb1d2 1451776_s_at 11.96 BC024546 MGI:1916782 1443798_at 14.38 BB207248 Pik3cd 1421834_at 11.96 NM_008846 Pip5k1a 1434950_a_at 14.35 BE995635 Armc8 1428268_at 11.86 AK018116 Psd2 1434034_at 14.27 BI905090 Cerk 1433589_at 11.85 BM238232 D6Wsu116e 1417279_at 14.26 NM_010585 Itpr1 1422409_at 11.84 NM_008237 Hes3 1450896_at 14.19 BM248774 Arhgap5 1423062_at 11.76 AV175389 Igfbp3 1425545_x_at 14.14 M86502 H2-D1 ; H2-L 1438035_at 11.73 BB748934 AW061290 1419017_at 14.14 NM_016869 Corin 1456631_at 11.65 AV272901 LOC381279 1449577_x_at 14.02 AK003186 Tpm2 1425440_x_at 11.61 BC011108 Slc41a3 1426923_at 14.02 BB130716 Hrb 1446441_at 11.55 BB126659 Slc20a1 1426500_at 13.95 BF462080 Icmt 1458918_at 11.52 BB552741 Slc12a8 1415948_at 13.89 BC027426 Creg1 1451602_at 11.5 BC025911 Snx6 1458129_at 13.86 BB559501 Rora 1452462_a_at 11.45 BC013339 Banp 1416302_at 13.81 BB125261 Ebf1 1418254_at 11.43 NM_019735 Apip 1420595_at 13.79 NM_007867 Dlx4 1452478_at 11.42 AY044451 Alpk2 1421996_at 13.77 NM_011547 Tcfap2a 1428064_at 11.41 BI248650 Centd2 1437987_at 13.73 BB389497 1455455_at 11.39 BB008997 Glt28d2 1441786_at 13.7 AW048005 1450914_at 11.37 BE986849 Ppp1r14b 1452890_at 13.66 AK009255 Ttll5 1455343_at 11.37 BF159528 Plekha7 1436690_at 13.55 BB494139 Lrba 1447282_at 11.36 BE979542 Chtf18 1418758_a_at 13.43 NM_011182 Pscd3 1416785_at 11.34 NM_027398 Kcnip1 1419999_at 13.4 C81413 Igbp1 1448799_s_at 11.24 NM_011885 Mrps12 1439959_at 13.31 AW488620 Fgf11 1457232_at 11.22 BE946365 Fbxl21 1438036_x_at 13.29 BB748934 AW061290 1438812_x_at 11.2 BB771589 Usp19 1447625_at 13.25 BB286270 E2f5 1434273_at 11.18 BG073439 A830073O21Rik 1439447_x_at 13.19 AV056887 Rpl37a 1438987_at 11.17 AV044525 4921509E07Rik 1421436_at 13.13 NM_008167 Grid2 1416645_a_at 11.16 NM_007423 Afp 1456934_at 13.11 BB177770 1426324_at 11.01 M33151 H2-D1 1416160_at 13.1 AI463873 Nr2f2 1416664_at 10.97 NM_023223 Cdc20 1418831_at 13.06 AW475993 Pkp3 1428345_at 10.96 BB229589 4932443D16Rik 1436867_at 13 BG795043 Srl 1450852_s_at 10.9 BQ173958 F2r 1432478_a_at 12.96 AK015966 Ibrdc3 1443187_at 10.89 AV235077 Rspo3 1416301_a_at 12.96 BB125261 Ebf1 1420653_at 10.89 NM_011577 Tgfb1 1452362_at 12.89 BB834440 Trim16 1453829_at 10.89 BE948719 2310007J06Rik 1442502_at 12.89 BB011593 Lrch1 1434036_at 10.72 AV024771 Mtss1 1426981_at 12.86 BI157485 Pcsk6 1450310_at 10.69 NM_133355 Grid2ip 1421435_at 12.86 NM_008167 Grid2 1456596_at 10.63 BB093996 6430550H21Rik 1439789_at 12.85 BQ177189 1453409_at 10.62 AK004156 Cgrrf1 1423536_at 12.67 BF148627 Strn3 1433581_at 10.62 AV309085 1190002N15Rik 1438073_at 12.66 AW047633 1457118_at 10.61 AV353605 6230417E10Rik 1439699_at 12.66 BB525237 Pgr 1421078_at 10.56 NM_053085 Tcf23 1451784_x_at 12.65 L36068 H2-D1 ; H2-K1 ; 1438151_x_at 10.55 BB544336 Zdhhc14 H2-L ; 1424709_at 10.52 AB016248 Sc5d LOC56628 1436399_s_at 10.52 BI901194 Nrk 1428636_at 12.62 AK015015 Steap2 1442015_at 10.49 BG070940 1460084_at 12.62 BB473929 1455165_at 10.45 BE335227 Rora 1433764_at 12.6 BB026221 Clec21 1457942_at 10.42 BI467159 Pdcl 1453936_at 12.6 BQ032796 1452095_a_at 10.37 AK005204 H47 1455775_at 12.55 AW988196 1439849_at 10.33 BB215381 1431241_at 12.29 AA692147 Chchd3 1433919_at 10.31 AV302111 Asb4 1422426_at 12.29 NM_020612 Cmar 1455637_x_at 10.29 BB465277 1442823_at 10.24 BB536648 1420667_at 8.751 BM117900 Doc2b 1451426_at 10.23 AF316999 D11Lgp2e 1418233_a_at 8.719 AV226526 Trappc5 1431167_at 10.16 AI646503 Dgkg 1451163_at 8.717 AF214013 Tinf2 1440827_x_at 10.15 BB018032 Sox5 1449461_at 8.67 NM_023462 Rbp7 1449872_at 10.14 NM_019960 Hspb3 1445218_at 8.661 BE955408 Vax2 1415887_at 10.12 NM_019678 Tfg 1436281_at 8.642 BB465277 1441622_at 10.05 AV374024 LOC433023 1437841_x_at 8.624 BB248249 Csdc2 ″AFFX-r2- 1438502_x at 8.618 AA030209 Rps17 ; Bs-phe-3_at″ 10.02 AFFX-r2-Bs- LOC383032 phe-3 1443197_at 8.608 BB501165 Wnt9b 1416158_at 10.02 AI463873 Nr2f2 1431073_at 8.594 AK015225 4930428J16Rik 1458753_at 9.948 BB548169 Gm941 1425028_a_at 8.571 BC024358 Tpm2 1422753_a_at 9.868 BG070121 Polr3k 1436112_at 8.555 BB128317 AI118078 1441025_at 9.865 BB333919 3110047P20Rik 1425891_a_at 8.555 AF329833 Grtp1 1422157_a_at 9.816 NM_008403 Itgb1bp1 1417147_at 8.535 NM_019833 B230317C12Rik 1432355_at 9.808 AK008780 2210039B01Rik 1452244_at 8.517 AK018128 6330406115Rik 1453060_at 9.804 AK018337 Rgs8 1439441_x_at 8.505 BB134767 Lats2 1423422_at 9.794 AV113827 Asb4 1417430_at 8.503 NM_007672 Cdr2 1424541_at 9.785 BC011320 Tmem70 1425779_a_at 8.469 AF326960 Tbx1 1450897_at 9.748 BM248774 Arhgap5 1444730_at 8.421 BB297867 Tlk1 1416131_s_at 9.717 BB188557 C920006C10Rik 1435517_x_at 8.385 BB465250 Ralb 1458395_at 9.717 BB811756 B930054O08 1434881_s_at 8.365 BM220945 Kctd12 1458595_at 9.712 AW907704 Pdzk6 1436895_at 8.353 BB182934 Centd1 1449876_at 9.684 NM_011160 Prkg1 1437392_at 8.352 AW123113 LOC433485 1443733_x_at 9.655 C85233 Pold3 1416398_at 8.346 BB474887 Mesdc1 1458677_at 9.609 BB517186 Entpd5 1426374_at 8.34 AK010825 2410166I05Rik 1428601_at 9.415 AK005628 1700003E16Rik 1444112_at 8.331 BQ174086 1449202_at 9.38 NM_011976 Sema4g 1459107_at 8.323 BG069378 Kcnh3 1434110_x_at 9.284 BF322785 Mup1 1448160_at 8.305 NM_008879 Lcp1 1430780_a_at 9.284 B1739353 Pmm1 1416121_at 8.303 M65143 Lox 1434014_at 9.27 BB291836 Atg4c 1416793_at 8.293 NM_019717 Arl6ip2 1427594_at 9.263 BC002224 Zfp697 ″AFFX-r2- 1455164_at 9.239 AV308092 Cdgap Bs-dap-M_at″ 8.268 AFFX-r2-Bs- 1429884_at 9.16 AK005172 Srgap2 dap-M 1441161_at 9.152 BB307791 B230216G23Rik 1424350_s_at 8.259 BE987427 Lpgat1 1442042_at 9.113 BG092211 1436728_s_at 8.218 BG071028 Rtel1 1451536_at 9.105 AK009195 Mtfr1 1447864_s_at 8.212 AV377712 Pogk 1419660_at 9.097 NM_025904 1600012F09Rik ″AFFX- 1425017_at 9.095 BB314809 Pcdhac1 DapX-M_at″ 8.187 AFFX-DapX- 1451147_x_at 9.037 BC016109 Csdc2 M 1435735_x_at 9.034 BB285733 H47 1460735_at 8.169 BM203457 Svil 1449307_at 9.014 NM_028146 2810427I04Rik 1423194_at 8.139 BM248774 Arhgap5 1429156_at 8.998 BF453953 2610036L11Rik 1452642_at 8.115 BE372017 Tmem16f 1430125_s_at 8.981 AK009256 Pqlc1 1460647_a_at 8.106 NM_010150 Nr2f6 1449403_at 8.978 NM_008804 Pde9a 1419647_a_at 8.096 NM_133662 Ier3 1450702_at 8.907 AJ306425 Hfe 1427940_s_at 8.082 BB046347 Mycbp 1448704_s_at 8.889 NM_024439 H47 1448812_at 8.055 NM_016677 Hpcal1 1440725_at 8.882 BB131092 Gpr63 1419614_at 8.039 BC021592 Pla2g12b 1450837_at 8.853 NM_031499 Prh1 1434064_at 8.026 BQ175677 BC061259 1439006_x_at 8.849 BB093996 6430550H21Rik 1437683_x_at 8.004 AV047585 Serf2 1444363_at 8.848 BB269445 1453528_at 7.985 AK005161 Lta4h 1428760_at 8.831 AW537061 Snapc3 1435208_at 7.966 AV327407 Dtx3l 1420058_s_at 8.83 AI480750 2410166I05Rik 1423535_at 7.959 BF148627 Strn3 1459300_at 8.784 BB226459 1420566_at 7.945 NM_025367 1447296_at 8.755 AW490725 Fbxo27 1435671_at 7.945 BB526605 Mipol1 1459592_a_at 8.755 BB752796 LOC547217 1456731_x_at 7.932 AV169424 Polr3k 1437914_at 7.923 BB104097 E2f6 1446786_at 7.197 BG073077 Aplp2 1450500_at 7.916 AW012751 Uhmk1 1449347_a_at 7.19 NM_021365 Xlr4b ; Xlr4a 1420666_at 7.908 BM117900 Doc2b 1455056_at 7.188 BM231903 Lmo7 1443766_x_at 7.895 BB311234 Rab11fip4 1419406_a_at 7.177 NM_016707 Bcl11a 1454842_a_at 7.884 AI853240 B3galnt2 1451353_at 7.171 AV378394 Tm6sf1 1426048_s_at 7.883 BC021623 Tcfap2a 1452451_at 7.168 AF240460 MGI:1929597 1445728_at 7.88 BB667663 1435491_at 7.167 BI076615 AI875089 1416159_at 7.878 AI463873 Nr2f2 1438501_at 7.166 AA030209 1430489_at 7.83 BB086802 5430416G10Rik 1455710_x_at 7.16 AI528732 Mtcp1 1416542_at 7.826 NM_009343 Phf1 1449079_s_at 7.154 NM_018784 St3gal6 1425197_at 7.813 BG076152 Ptpn2 1417653_at 7.141 NM_013645 Pvalb 1440735_at 7.812 BB283168 Polr3k 1419836_at 7.105 AU040583 1425177_at 7.809 AF237702 Shmt1 1435594_at 7.1 AV334690 Arl6ip2 1454161_s_at 7.733 AK002308 0610007P14Rik 1456251_x_at 7.095 BB132602 Bzrp 1439425_x_at 7.729 AV100801 BC024814 1451190_a_at 7.063 BC025837 Sbk1 1418909_at 7.722 NM_013848 1417344_at 7.009 BE915256 2900064A13Rik 1450235_at 7.718 NM_015759 Fgd3 1455850_at 7.006 BG073353 2310003H01Rik 1420304_x_at 7.709 AV128236 1437308_s_at 7 AV024285 F2r 1456722_at 7.685 BI966456 Chrdl1 1458358_at 6.994 BB402666 Pank2 1452827_at 7.657 AK005178 1500009C09Rik 1417121_at 6.991 NM_008068 Gabra6 1449176_a_at 7.637 NM_007832 Dck 1447753_at 6.987 BB391093 Cdc37l1 1457341_at 7.636 BE991676 1431172_at 6.987 BB620704 Orc4l 1417960_at 7.623 NM_007755 Cpeb1 1431299_a_at 6.979 AK009340 2310014H01Rik 1449584_at 7.562 NM_138650 Dgkg 1420059_at 6.964 AI480750 2410166I05Rik 1438698_at 7.547 AV305379 4632425D07Rik 1418932_at 6.936 AY061760 Nfil3 1433623_at 7.546 BE629588 Zfp367 1443714_at 6.935 AI451920 1424902_at 7.481 AF378760 Plxdc1 1423055_at 6.917 NM_010942 Nsg1 1451796_s_at 7.479 AF109137 Hdc 1429204_at 6.905 AK013788 2900075A18Rik 1443846_x_at 7.477 BB402666 Pank2 1426032_at 6.897 AF289078 Nfatc2 1421833_at 7.473 NM_008846 Pip5k1a 1456337_at 6.896 AV291818 Centd1 1458536_at 7.455 BB097972 Ccni 1440700_a_at 6.895 BB227996 Arhgef18 1422941_at 7.449 NM_053116 Wnt16 1419880_x_at 6.893 AA204020 1455448_at 7.415 BG804066 MGI:2677061 1449621_s_at 6.892 AW121720 Thsd1 1436322_a_at 7.391 BE952576 2810001A02Rik 1426189_at 6.89 AF357887 Dusp15 1426937_at 7.383 AK018128 6330406I15Rik 1455160_at 6.882 BM220421 2610203C20Rik 1441271_at 7.371 C78231 Idh3b 1460462_at 6.871 AK012903 Med18 1458328_x_at 7.36 BE993003 3110007P09Rik 1427556_at 6.869 BC019408 Mylk2 1424692_at 7.351 BC004753 2810055F11Rik 1419742_at 6.862 NM_026091 1700037H04Rik 1416632_at 7.335 BC011081 Mod1 1437935_at 6.85 BB821151 4930486G11Rik 1425737_at 7.324 BC002195 2510016G02Rik 1454692_x_at 6.844 BB722680 1454866_s_at 7.313 BQ176424 Clic6 1437942_x_at 6.843 AV100095 Tube1 1417876_at 7.307 AF143181 Fcgr1 1424216_a_at 6.831 U52197 Papola 1419422_at 7.301 BB706784 Pkd2l2 1436829_at 6.82 BB667112 Trim67 1428948_at 7.297 BE952741 5730414M22Rik 1428452_at 6.813 AK012812 2810025M15Rik 1438967_x_at 7.297 AV259665 Amhr2 1432059_x_at 6.775 AK017143 5031425E22Rik 1442614_at 7.297 BE945253 Il1rap 1453554_a_at 6.771 AK009297 Wdr33 1424034_at 7.297 BI660199 Rora 1452016_at 6.759 BC026209 Alox5ap 1430827_a_at 7.271 AV301702 Ptk2 1434877_at 6.746 AI152800 Nptx1 1428197_at 7.263 AK020159 Tspan9 1449836_x_at 6.743 NM_007546 Biklk 1431226_a_at 7.26 AK013203 Fndc4 1428604_at 6.739 AK011986 2610305D13Rik 1459875_x_at 7.25 AV333371 1418028_at 6.735 NM_010024 Dct 1432271_a_at 7.247 AK012764 Dcun1d5 1425178_s_at 6.732 AF237702 Shmt1 1436528_at 7.222 AI842353 Kazald1 1438948_x_at 6.73 AV101079 Bzrp 1426922_s_at 7.212 BB130716 Hrb 1442376_at 6.717 BB560441 Ablim1 1430490_at 7.21 AK017409 1424784_at 6.709 AV047635 1700029I01Rik ; 1423845_at 7.202 BC016109 Csdc2 LOC433791 ; LOC545705 1419756_at 6.348 NM_138650 Dgkg 1427878_at 6.695 AK002512 0610010O12Rik 1416848_at 6.343 NM_025401 Ubl5 1457330_at 6.682 BB014986 Semcap2 1420774_a_at 6.34 NM_026358 4930583H14Rik 1436141_at 6.681 BM934616 2610510L01Rik 1417222_a_at 6.34 NM_133739 2310075C12Rik 1442099_at 6.681 BM227490 Usp31 1430866_at 6.339 BB432681 4921537D05Rik 1437642_at 6.674 BB243375 Hrbl 1436087_at 6.328 BQ176414 Dpp10 1450168_at 6.666 NM_025971 Ankrd12 1439117_at 6.328 AU067755 Clmn 1436536_at 6.665 AW108488 C730015A04Rik 1415854_at 6.321 BB815530 Kitl 1425701_a_at 6.662 AF350047 Rgs3 1431229_at 6.286 AK019361 C030032C09Rik 1442791_x_at 6.657 BF459282 6720407P12Rik 1431356_at 6.282 AK019889 6430710C18Rik 1454939_at 6.643 BB268102 Phf20l1 1460262_a_at 6.274 NM_026091 1700037H04Rik 1421360_at 6.636 NM_030266 Inpp4a 1450115_at 6.267 NM_008139 Gnaq AFFX-ThrX- 6.611 AFFX-ThrX-3 1418247_s_at 6.246 BG277926 Rbm9 3_at 1439837_at 6.242 BE136147 Tnrc15 1450537_at 6.601 AF196480 Mid2 1430519_a_at 6.228 AK007767 Cnot7 1457894_at 6.601 BB284616 1422313_a_at 6.222 NM_010518 Igfbp5 1433792_at 6.597 AW491344 Nrip2 1439825_at 6.209 BB705351 Dtx3l 1416846_a_at 6.59 NM_018884 Pdzn3 1448613_at 6.205 NM_007899 Ecm1 1459842_x_at 6.588 AV035368 Nubp2 1460479_at 6.201 AK020726 A330094K24Rik 1433429_at 6.584 BB309245 Pigs 1423020_at 6.188 NM_053120 1431279_s_at 6.569 AK016145 Ttll5 1424604_s_at 6.18 BC026981 Sumf1 1420020_at 6.559 AU022339 Suz12 1453301_a_at 6.178 AK008083 2010004B12Rik 1431421_x_at 6.55 BI082843 2610524G07Rik 1450622_at 6.176 NM_009954 Bcar1 1454219_at 6.543 AK013295 Dnajc2 1418760_at 6.168 AB030503 Rdh11 1431088_at 6.536 BQ286886 Foxp4 1439479_at 6.16 C78577 Lct 1451840_at 6.535 BG261945 Kcnip4 1424846_at 6.159 BC027174 BC027174 1431293_a_at 6.519 AK012260 1110019C08Rik 1458268_s_at 6.133 AI649005 Igfbp3 1439078_at 6.513 BB037068 Klhl4 1418007_at 6.125 BM932567 1810007M14Rik 1435621_at 6.513 BQ175346 Mlstd1 1435092_at 6.125 AV328143 Arl4 1457290_at 6.49 BB127590 Mab21l1 1448487_at 6.124 NM_008515 Lrrfip1 1436347_a_at 6.485 BB501229 5530601H04Rik 1415791_at 6.122 NM_030564 Rnf34 1458320_at 6.47 BB012140 1427593_at 6.114 BB620112 Trim8 1454638_a_at 6.464 AW106920 Pah 1437762_at 6.106 BB130995 Rab39 1457256_x_at 6.463 BB530125 Ptch2 1453024_at 6.105 AK014128 Wdr37 1429583_at 6.46 BE865033 LOC554362 1428344_at 6.093 BB229589 4932443D16Rik 1433836_a_at 6.447 AV365503 8430408G22Rik 1448441_at 6.086 NM_016904 Cks1b 1427174_at 6.433 BM235840 3100002L24Rik 1423854_a_at 6.085 BC008101 Rasl11b ; LOC329575 ; 1449310_at 6.079 BC005440 Ptger2 LOC433520 ; 1449896_at 6.07 NM_053015 Mlph LOC433523 ; 1435947_at 6.069 BQ175669 LOC433531 ; 1425439_a_at 6.06 BC011108 Slc41a3 LOC545489 ; 1460382_at 6.06 BC020535 BC020535 LOC545490 ; 1437631_at 6.038 BB188374 Kcnip4 LOC545491 ; 1418202_a_at 6.031 NM_011717 Wiz LOC545492 1435238_x_at 6.028 AV004950 2310009A05Rik 1423371_at 6.417 BF577544 Pole4 1444601_at 6.02 AI463033 1419399_at 6.416 AW553649 Mttp 1459468_at 5.999 C79743 C79743 1456926_at 6.4 BB049544 Rims4 1453591_at 5.98 AK017626 5730437N04Rik 1442160_at 6.4 AV360034 7530404M11Rik 1431707_a_at 5.976 AK016645 Pscd3 1451601_a_at 6.399 BC025823 BC011467 1451085_at 5.974 BC019462 C030006K11Rik 1436661_at 6.394 BQ174532 Dpp10 1426544_a_at 5.974 BB118847 Ttc14 1430803_at 6.391 BB074761 2810403D23Rik 1456210_at 5.967 AV222628 1455080_at 6.376 BB375209 Ppp1r16b 1450506_a_at 5.914 NM_026531 Isg20l1 1431642_at 6.373 AV122850 Eif2s3y 1437262_x_at 5.902 AA940256 Bcas2 1452237_at 6.361 BB130716 Hrb 1430763_at 5.9 BB016642 4930563E22Rik 1424191_a_at 6.354 BC019770 Tmem41a 1449259_at 5.896 BB349707 Rab3d 1419305_a_at 5.879 NM_025386 Fbxo36 LOC546355 1424092_at 5.871 BC017137 Epb4.1 1423668_at 5.537 BC021423 Zdhhc14 1417933_at 5.857 NM_008344 Igfbp6 1434165_at 5.523 BQ176424 Clic6 1421281_at 5.851 Z36357 Gabra1 1427170_at 5.521 BF318721 Psma8 1437594_x_at 5.835 BB376188 Pigt 1436160_at 5.516 BB150142 4732407F15Rik 1423229_at 5.835 BM217803 Inpp5e 1423671_at 5.515 AF370126 Dner 1435204_at 5.83 BG065273 Hrmt1l4 1457652_x_at 5.509 BB477203 1449072_a_at 5.828 NM_026526 2510005D08Rik 1438992_x_at 5.507 AV314773 Atf4 1433134_at 5.826 AK017998 5830448L01Rik 1433787_at 5.491 AI841091 B230343H07Rik 1430879_at 5.825 BB283753 C030008P14Rik 1435138_at 5.456 AV016797 Tmem28 1440708_at 5.803 BM121854 Myh9 1418796_at 5.446 NM_009131 Clec11a 1427981_a_at 5.79 AY033912 Csad 1451365_at 5.445 BC025619 Rbm19 1449509_at 5.788 NM_011353 Serf1 1456563_at 5.443 BB769119 1437371_at 5.785 BB008528 9930021J17Rik 1451418_a_at 5.428 BC023083 Spsb4 1451975_at 5.783 BC005638 2810453I06Rik 1421799_at 5.423 NM_011910 Uts2 1439152_at 5.78 BE985292 BC052066 1422093_at 5.421 NM_009522 Wnt3a 1455755_at 5.776 BB026554 Gm88 1444380_at 5.412 AI844869 AI844869 1437595_at 5.762 BB531414 E030010A14Rik 1426552_a_at 5.412 BB772866 Bcl11a 1424229_at 5.748 BC006704 Dyrk3 1457472_at 5.407 BB782031 Tnrc15 1456313_x-at 5.744 BB257397 Mrpl28 1418386_at 5.384 NM_026526 2510005D08Rik 1429971_at 5.731 BE948556 Txnrd2 1435277_x_at 5.377 AV156640 Nme1 1435270_x_at 5.72 BF730076 2510005D08Rik 1436454_x_at 5.372 BB393998 Fen1 1444141_at 5.711 BB828008 Snx13 1421746_a_at 5.366 NM_015796 Fbxo17 1449287_at 5.695 NM_011481 Srms 1432161_a_at 5.363 AK006988 4930428J16Rik 1451363_a_at 5.686 BC008266 Dennd2d 1430156_at 5.351 AK015855 4930520O04Rik 1457433_x_at 5.685 BB187816 Zfp120 1449910_at 5.345 NM_029813 2210418O10Rik 1420150_at 5.685 AI596360 Spsb1 1419739_at 5.342 AK003186 Tpm2 1447771_at 5.679 AW050081 1419913_at 5.339 AW557906 Strap 1418890_a_at 5.678 BB349707 Rab3d 1460652_at 5.338 NM_007953 Esrra 1448986_x_at 5.675 NM_010062 Dnase2a 1438014_at 5.337 BE984852 Mrpl34 1422847_a_at 5.674 AF251036 Prkcd 1418583_at 5.337 NM_025798 Hint3 1439035_at 5.673 BG075830 Zfp322a 1423286_at 5.331 AA016422 Cbln1 1457961_at 5.667 BB713538 1449209_a_at 5.323 AB030503 Rdh11 1449897_a_at 5.664 NM_010839 Mtcp1 1459941_at 5.323 BB096900 4933402J24Rik 1437820_at 5.654 BB040642 Fkhl18 1426901_s_at 5.313 BB657856 Camta2 1440242_at 5.647 BB356428 C030014K22Rik 1419851_at 5.305 W13854 Slc4a8 1428216_s_at 5.645 BB609428 Tomm7 1427997_at 5.305 BG064890 1110007M04Rik 1449362_a_at 5.642 NM_016713 Mink1 1427318_s_at 5.302 BI555209 Dysf ; Fer1l3 1458347_s_at 5.635 BB414224 Tmprss2 1429726_at 5.301 AK004684 Slc16a9 1423384_s_at 5.633 BF181445 Tex261 1426856_at 5.293 BM200015 Hsdl2 1430590_at 5.628 BG228878 D3Ertd751e 1453968_at 5.282 AK019523 4833439F03Rik 1428447_at 5.611 AK017734 Tmem14a 1448999_at 5.281 AV226526 Trappc5 1419421_at 5.609 NM_031158 Ank1 ″AFFX-r2- 1424969_s_at 5.604 BC027189 Upp2 Bs-thr-3_s_at″ 5.276 AFFX-r2-Bs- 1438793_x_at 5.601 BB222127 1200007D18Rik thr-3 1458132_at 5.592 BB429249 Cnnm2 1441883_at 5.266 AV092359 0610010O12Rik 1435970_at 5.588 AU035920 Nlk 1455599_at 5.264 AV220135 AI850995 1418389_at 5.585 NM_133703 2810453I06Rik 1450965_at 5.263 BF181445 Tex261 1455281_at 5.581 BB520667 Wdr33 1423048_a_at 5.263 BB400304 Tollip 1422645_at 5.576 AJ306425 Hfe 1444703_at 5.242 BB525928 2810403D21Rik 1420887_a_at 5.569 NM_009743 Bcl2l1 1459871_x_at 5.241 AV349132 2-Mar 1436906_at 5.56 BF781579 Rnf166 1423686_a_at 5.241 BC016234 1110020C13Rik 1434382_at 5.547 AV083936 Tde21 1437857_at 5.234 AV367203 Dpy19l3 1436879_x_at 5.544 AV124668 Afp 1428472_at 5.232 AK003707 Spsb1 1437711_x_at 5.541 BB519474 Odc1 ; 1449762_at 5.231 AV047896 LOC545783 ; 1436897_at 5.227 BE225764 Mfhas1 1419351_a_at 5.226 BC003916 0610007P06Rik 1417677_at 5.037 NM_010098 Opn3 1425330_a_at 5.226 AJ271836 Ppm1b ; 1459896_at 5.036 AW911766 Pogk LOC433336 1439574_at 5.029 BI737205 1110020A21Rik 1416521_at 5.225 NM_009156 Sepw1 1438549_a_at 5.007 BB020681 Srr 1442711_at 5.222 BB129490 A530065E19Rik 1456467_s_at 5.006 BB389081 Nlk 1452169_a_at 5.221 BC014860 Dgkz 1458662_at 4.999 BB546123 Daam1 1429881_at 5.221 AK018058 Arhgap15 1455052_a_at 4.998 BI153133 2410129H14Rik 1429092_at 5.212 AK009497 Vkorc1l1 1454225_s_at 4.984 AK012700 D3Ertd751e 1436189_at 5.207 AV380561 1421330_at 4.979 NM_019933 Ptpn4 1435516_x_at 5.204 BB752007 Rtel1 1448740_at 4.975 NM_021329 2400006H24Rik 1418234_s_at 5.202 NM_026602 Bcas2 1451395_at 4.972 BC021492 D5Bwg0834e 1434177_at 5.199 AI551117 Ece1 1447097_at 4.966 BG068083 1437137_at 5.197 AV280875 6430550H21Rik 1420010_at 4.966 C80083 LOC574418 1448344_at 5.189 NM_011295 Rps12 ; 1456056_a_at 4.963 AV338866 D6Wsu116e LOC432865 ; AFFX-LysX- 4.957 AFFX-LysX-3 LOC546370 ; 3_at LOC546371 ; 1460203_at 4.95 NM_010585 Itpr1 LOC546372 ; 1429620_at 4.949 BB040330 8430406I07Rik LOC546373 1442218_at 4.945 BM201230 Map3k9 1451881_at 5.186 BC027044 1417335_at 4.94 NM_017465 Sult2b1 1437489_x_at 5.184 BB497590 Sdhd 1435875_at 4.934 AV223660 Prkab2 1429972_s_at 5.183 BE948556 Txnrd2 1442073_at 4.931 BQ266693 1432579_at 5.177 AA544511 Rshl2 1459696_at 4.928 AI844428 9330186A19Rik 1439150_x_at 5.166 BB480256 Grtp1 1420148_at 4.923 AA589629 Slc6a6 1451596_a_at 5.161 AF068749 Sphk1 1448661_at 4.922 NM_008874 Plcb3 1447397_at 5.158 BB733030 1700129I04Rik 1429005_at 4.919 BB107412 Mfhas1 1434664_at 5.151 BI153133 2410129H14Rik 1429664_at 4.917 AI464135 Cdkl1 1452274_at 5.13 BG976649 Tex27 1424848_at 4.915 U09383 Kcnma1 1422627_a_at 5.121 BF581250 Mkks 1447552_s_at 4.913 AV169798 Catnd2 1422490_at 5.116 AV144704 Bnip2 1455885_at 4.902 AV238106 6530401C20Rik 1418259_a_at 5.112 NM_009849 Entpd2 1451387_s_at 4.894 BC021463 0610039D01Rik 1432343_at 5.111 AK015168 4930419G24Rik 1453993_a_at 4.883 AK014659 Bnip2 1426880_at 5.106 BM250266 BC026657 1453077_a_at 4.882 AK016168 Snapc3 1438769_a_at 5.105 BF719766 MGI:1925112 1451525_at 4.877 BG064038 Arhgap12 1452640_at 5.104 AK014019 3110007F17Rik 1454824_s_at 4.865 BB699957 Mtus1 ; LOC546367 ; 1438623_x_at 4.86 AV038578 Rbx1 LOC546368 1436681_x_at 4.857 BE199670 0610009D07Rik 1436475_at 5.103 AI527205 Nr2f2 1440882_at 4.852 BB750940 Lrp8 1435237_at 5.098 AV004950 2310009A05Rik 1435755_at 4.85 BB609468 1110001A16Rik 1419738_a_at 5.094 AK003186 Tpm2 1438656_x_at 4.831 AV157500 Timm17b 1428296_at 5.085 AV102258 Polr2l 1450648_s_at 4.83 NM_010379 H2-Ab1 1418008_at 5.085 BM932567 1810007M14Rik 1439509_at 4.826 BE985592 2900008C10Rik 1436042_at 5.084 BI648366 Tln1 1449012_s_at 4.818 BC027164 Fndc4 1445074_at 5.078 BB519491 1436869_at 4.817 AV304616 Shh 1455601_at 5.072 BB507994 1439388_s_at 4.813 BB251739 Bcar1 1454898_s_at 5.072 AU016407 4833421E05Rik 1428662_a_at 4.809 AK009007 MGI:1916782 1417946_at 5.058 NM_134130 Abhd3 1429988_at 4.804 AK002713 Zfp235 1452768_at 5.058 AK013971 Tex261 1436614_at 4.804 AI843639 AI843639 1434291_a_at 5.054 AA709993 Serf1 1419112_at 4.803 NM_008702 Nlk 1437677_at 5.053 BG063913 AI449595 1449983_a_at 4.799 NM_020282 Nqo2 1428822_a_at 5.053 BB815011 Snx24 1441481_at 4.799 AV262974 Mfap3l 1421083_x_at 5.042 NM_011793 Banf1 1460030_at 4.796 BB355811 Hecw1 1426382_at 5.042 AJ271833 Ppm1b 1457644_s_at 4.785 BB554288 Cxcl1 1437980_at 5.041 BB814947 9130230N09Rik 1440886_at 4.781 BE949427 Cdc37l1 1449471_at 5.04 NM_021452 Kcnmb4 1428309_s_at 4.78 AK003410 Pdrg1 1438408_at 5.039 BB131927 5730467H21Rik 1456036_x_at 4.777 AV003026 Gsto1 1425149_a_at 4.775 BC006578 Pdcl 1453782_at 4.562 BI737125 3021401C12Rik 1419400_at 4.761 AW553649 Mttp 1440603_at 4.555 BB073590 1418996_a_at 4.759 BC021522 4930469P12Rik 1451370_at 4.549 BC027414 1428607_at 4.754 AK010060 1451607_at 4.544 AB039276 Klk21 1423318_at 4.75 AK012795 Rad18 1443073_at 4.543 BB355954 LOC545681 1421295_at 4.749 NM_031258 Chrdl1 1459890_s_at 4.539 C79326 1110008P14Rik 1449027_at 4.747 AF378088 Rhou 1417912_at 4.536 NM_025318 0610009E20Rik ″AFFX- 1438619_x_at 4.533 BB318221 Zdhhc14 PheX-3 at″ 4.746 AFFX-PheX-3 1436698_x_at 4.529 AV167328 BC054438 1430203_at 4.746 BG067256 Usp16 1421640_a_at 4.528 NM_011529 Tank 1434732_x_at 4.745 AV044898 Tomm7 1453386_at 4.528 AK008612 Tusc1 1420655_at 4.729 NM_054088 Adpn 1424944_at 4.526 BC024853 Pcp2 1417095_a_at 4.729 NM_015765 Hspa14 1448954_at 4.523 NM_020610 Nrip3 1420556_at 4.728 NM_011025 Oxt 1422153_a_at 4.522 NM_026853 Asb11 1458466_at 4.726 AV338634 Ppgb 1451477_at 4.518 AV047635 LOC545705 ; 1444475_at 4.721 BE983052 1810009N02Rik LOC545706 1440226_at 4.717 BB088782 BC029103 1437067_at 4.517 BM228625 Phtf2 1440995_at 4.709 BE946005 6430531B16Rik 1430616_at 4.513 BB185833 4930528A17Rik 1430686_at 4.705 AK014719 4833418N02Rik 1434032_at 4.511 BQ175381 1429114_at 4.699 AV276619 Sestd1 1419808_at 4.506 AU022131 Cog4 1454663_at 4.698 BQ176989 Eif5 1428488_at 4.498 BB646655 Pigk 1417590_at 4.693 NM_024264 Cyp27a1 1436926_at 4.491 AV333667 Esrrb 1460648_at 4.684 NM_010150 Nr2f6 1423219_a_at 4.49 BM951442 Mrpl49 1439012_a_at 4.683 BB030204 Dck 1421280_at 4.485 Z36357 Gabra1 1420888_at 4.673 NM_009743 Bcl2l1 1417163_at 4.473 NM_022019 Dusp10 1415956_a_at 4.668 NM_011049 Pctk1 1416132_at 4.467 BB188557 C920006C10Rik 1433700_at 4.667 BB477807 4933433P14Rik 1450770_at 4.465 BC023359 3632451O06Rik 1449534_at 4.661 NM_011517 Sycp3 1456500_at 4.465 AV232784 Aph1b 1435523_s_at 4.656 BB779140 2700089E24Rik 1448586_at 4.461 NM_015765 Hspa14 1456097_a_at 4.653 BB830191 Itgb3bp 1422897_at 4.457 NM_009203 Slc22a12 1434763_at 4.646 BF457736 A730041O15Rik 1451272_a_at 4.455 BC016117 2510010F15Rik 1426035_at 4.646 BC026459 LOC574418 1458638_at 4.451 AI852796 Mtdh 1440663_at 4.634 BB322506 Xpo4 1426496_at 4.447 AK017695 Wdr55 1460553_at 4.623 BF466605 1700025K23Rik 1449752_at 4.445 AI596360 4930422J18Rik 1449021_at 4.618 NM_026308 Rpp21 1428996_at 4.443 AK014774 Gm1705 1437213_at 4.618 BG070110 Nudt21 1440883_at 4.439 BM125576 Usp6nl 1431072_a_at 4.614 BB865814 2610529H08Rik 1422683_at 4.428 NM_022986 Irak1bp1 1438947_x_at 4.613 BB459744 Sema3f 1452291_at 4.426 AV375176 Centd1 1441839_s_at 4.61 BB250824 BC024814 1441960_x_at 4.423 AV267590 5730494M16Rik 1436294_at 4.608 AV337707 Ankrd29 1421230_a_at 4.416 BI696168 Msi2h 1450752_at 4.604 NM_009989 Cyct 1433847_at 4.415 BB098407 D330017J20Rik 1434135_at 4.602 BB107552 B3galnt2 1430521_s_at 4.412 AW548480 Cpne8 1423005_a_at 4.597 NM_019585 Espn 1456727_a_at 4.41 AW413676 Csnk1d 1434777_at 4.596 BG064871 Lmyc1 1433927_at 4.408 AW552571 E430026A01Rik 1423833_a_at 4.592 BC018324 Brp44 1453567_s_at 4.404 AK014997 2810441K11Rik 1456782_at 4.589 BB525604 D1Ertd251e 1424847_at 4.403 M35131 Nefh 1422293_a_at 4.587 NM_134112 Kctd1 1419380_at 4.403 NM_033327 Zfp423 1454119_at 4.587 AK010697 Miknk1 1437810_a_at 4.4 AV311770 Hbb-bh1 1455890_x_at 4.586 BB709603 Smpn ; 1451432_x_at 4.399 BC022729 2010004B12Rik LOC545062 1446566_at 4.397 BB461266 Ap2b1 1451377_a_at 4.583 BC025501 Aaas 1417740_at 4.394 BB756370 Cdc37l1 1435966_x_at 4.579 BE953095 Mrpl13 1425780_a_at 4.393 BC024352 0610041E09Rik 1418891_a_at 4.577 BB349707 Rab3d 1451998_at 4.391 BC024597 4930485D02Rik 1454102_at 4.57 AK017143 5031425E22Rik 1417035_at 4.391 BI134670 Sac3d1 1433462_a_at 4.564 BB208212 Pi4k2a 1432096_at 4.39 AK010671 Snrpn 1436889_at 4.564 BQ268470 Gabra1 1447927_at 4.388 BG092512 Mpa2l 1457395_at 4.381 AW553048 5330421F07Rik 1457545_at 4.321 BB110572 Shh 1428308_at 4.373 AK003410 Pdrg1 1435956_at 4.317 BB277380 A930004K21Rik 1434249_s_at 4.371 BB083438 1460133_at 4.315 BF607205 Efna5 1423874_at 4.368 BC019463 Wdr33 1437637_at 4.315 BE852433 Phtf2 1424262_at 4.364 BC024599 2810003C17Rik 1452238_at 4.311 BB130716 Hrb 1428568_at 4.363 BB376573 B230217C12Rik 1436060_at 4.31 BB480659 0710005M24Rik 1435510_at 4.363 BB283101 Ppm1h 1452374_at 4.309 BB315154 Zfp322a 1454572_at 4.352 AK013093 2810414N06Rik 1451385_at 4.308 BC010826 2310056P07Rik 1423869_s_at 4.352 AF349659 Txnrd3 1421468_at 4.29 NM_008426 Kcnj3 1451548_at 4.35 BC027189 Upp2 1449557_at 4.289 NM_025904 1600012F09Rik 1418716_at 4.347 AK004037 Mrps25 1421985_a_at 4.288 AI449084 Eif4e2 1426558_x_at 4.334 BB283527 3100002L24Rik 1448699_at 4.286 NM_025317 Mrpl54 1420596_at 4.332 NM_007583 Cacng2 1450095_a_at 4.283 NM_025421 Acyp1 1441453_at 4.328 BB341122 B930096L08Rik 1423495_at 4.272 BE952632 Decr2 1454086_a_at 4.324 AK013416 Lmo2 1427039_at 4.271 AF057285 Epn1 1429087_at 4.323 AK005245 1110054O05Rik 1416407_at 4.269 AI323543 Pea15 1456163_at 4.323 AV127670 2700049P18Rik 1436349_at 4.267 BI408855 2700094K13Rik

TABLE 21 Probesets used as negative controls in the comparison of BACarray samples with the reference sample. GeneSymbol = official gene symbol; ProbeSymbol = Affymetrix probe identification corresponding to gene symbol. Probesets with an average expression over 100 in the reference sample were plotted. Gene Probe Gene Probe Gene Probe Gene Probe Symbol Symbol Symbol Symbol Symbol Symbol Symbol Symbol Cnp1 1418980_a_at Glul 1426235_a_at Apoe 1432466_a_at Slc1a3 1426340_at Cnp1 1437341_x_at Glul 1426236_a_at Apoe 1444108_at Slc1a3 1426341_at Cnp1 1449296_a_at Mbp 1419646_a_at Laptm5 1417721_s_at Slc1a3 1439072_at Cnp1 1460032_at Mbp 1433532_a_at Laptm5 1426025_s_at Slc1a3 1440491_at Fthfd 1424400_a_at Mbp 1436201_x_at Laptm5 1436905_x_at Slc1a3 1443749_x_at Fthfd 1424401_at Mbp 1451961_a_at Laptm5 1447742_at Slc1a3 1452031_at Galc 1420547_at Mbp 1454651_x_at Laptm5 1459841_x_at Vim 1438118_x_at Galc 1449900_at Mbp 1456228_x_at Slc1a2 1439940_at Vim 1450641_at Galc 1452907_at Mog 1448768_at Slc1a2 1451627_a_at Vim 1456292_a_at Gfap 1426508_at Plp 1425467_a_at Slc1a2 1457800_at Gfap 1426509_s_at Plp 1425468_at Slc1a2 1458314_at Gfap 1440142_s_at Plp 1451718_at Slc1a2 1459014_at

Example 13 Physiological Evaluation of TRAP Methodology Results

1) Overview

In order to evaluate the physiological significance of differential expression of genes associated with GABA_(A) receptors in D1 striatonigral of mice treated with cocaine, mice were chronically treated with cocaine or vehicle (saline) and then GABA_(A) receptor function was assayed electrophysiologically.

2) Brain Slice Preparation for Optical/Electrophysiological Study

Slices were obtained from 32- to 37-day-old BAC D1 or BAC D2 soluble eGFP-expressing transgenic mice (S. Falcon, R. Gentleman, Bioinformatics 23, 257-8 (2007)). All animals were handled in accord with Northwestern University ACUC and NIH guidelines. For chronic cocaine studies, mice were injected with 20 mg/kg cocaine or 100 μl saline once daily for fifteen days and brain slices were prepared 4 h after the last injection, as in the mRNA studies. Coronal slices containing the striatum were prepared at a thickness of 250 μm. The mice were deeply anesthetized with ketamine and Xylazine, transcardially perfused with oxygenated, ice cold, artificial cerebral spinal fluid (ACSF), and decapitated. Brains were rapidly removed and sectioned in oxygenated, ice-cold, ACSF using a Leica VT1000S vibratome (Leica Microsystems, Germany). The ACSF contained the following (in mM): 126 NaCl, 3 KCl, 2 CaCl2, 1 MgCl2, 25 NaHCO3, 1.25 NaH2PO4, and 15.6 D-(+)-glucose. Unless otherwise noted, all chemicals and reagents were obtained from Sigma (St. Louis, Mo.). The slices were transferred to a holding chamber in which they were completely submerged in ACSF at 35° C. for 1 h, after which they were stored at room temperature (22° C.-23° C.) until whole-cell recording. All ACSF solutions were bubbled continuously with 95% O2 and 5% CO2 to maintain oxygenation and a ph≈7.4, and periodically checked to ensure ≈300 mOsm/l.

3) Electrophysiological Analysis of GABAergic Synaptic Events

Whole-cell voltage-clamp recordings were performed using standard techniques. Individual slices were transferred to a submersion-style recording chamber on Olympus Optical (Melville, N.Y.) BX50WI microscope and continuously superfused with ACSF at a rate of 2-3 ml/min at 22° C.-23° C. Whole-cell voltage-clamp recordings were performed on striatal medium spiny neurons detected in the slice with the help of an infrared-differential interference contrast (IR-DIC) video microscope with an Olympus OLY-150 camera/controller system (Olympus, Japan). The following were added to the superfusion medium for all experiments to isolate mIPSCs: 50 μM 2-amino-5-phosphonopentanoic acid (AP-5, Tocris Cookson, Ellisville, Mo.) to block NMDA glutamate receptors, 5 μM 1,2,3,4-Tetrahydro-6-nitro2,3-dioxo-benzo quinoxaline-7-sulfonamide (NBQX) to block AMPA/kainite glutamate receptors, and 1 μM tetrodotoxin (TTX, Alomone Labs, Jerusalem, Israel) to block sodium channels. Patch electrodes were made by pulling Sutter BF150-86-10 glass on a P-97 Flaming/Brown micropipette puller (Sutter Instrument, Novato, Calif.) and fire polished before recording. Pipette resistance was typically 2.5-4 MΩ after filling with an internal solution containing the following (in mM): 140 CsCl, 1.5 MgCl2, 10 HEPES, 0.1 BAPTA-Cs, 5 QX-314, 2 ATP-Na2, 0.4 GTP-Na2, pH 7.25-7.3 adjusted with CsOH, 270-280 mOsm/L. Miniature IPSCs were recorded with a Multiclamp 700 A amplifier, a Digidata 1322 A 16-bit data acquisition system, and pClamp software version 8.2 (Molecular Devices, Union City, Calif.) in gap free mode. Neurons were voltage-clamped at −80 mV and allowed to reach a stable baseline (≈5 min) before mIPSCs were recorded for 7 min. Mini Analysis (Synaptosoft Inc., Fort Lee, N.J.) was used to analyze mIPSC amplitude, frequency, 10-90% rise time, decay time, and non-stationary noise analysis. A threshold of 5 times the root mean square baseline noise level (commonly≈20-25 pA) was set for event detection. Records were then visually inspected and all events triggered by noise were discarded. Frequency analysis was carried out on all mIPSCs that met threshold criteria. Events were then selected for amplitude, 10-90% rise time, and decay time analysis on the following criteria: 1) Events with 10-90% rise times faster than 1 ms were selected to minimize space clamp errors and electrotonic filtering; 2) Events with decay times faster than 50 ms were selected to minimize events in which multiple events precluded accurate decay time measurement. For non-stationary noise analysis, the same criteria for amplitude and mIPSCs kinetics were used followed by these additional criteria: 3) No multiple events in which the decay did not return to baseline; 4) Events had to have a stable baseline before the rise and after the end of the decay. Mean values of mIPSC measures were compared between groups with a t-test or Mann-Whitney rank sum test using Sigma Stat 3.0 (Systat Software Inc., Richmond, Calif.). Pooled data are presented as means±SE and box plots using IGOR 5.00 (WaveMetrics, Lake Oswego, Oreg.).

4) 2-Photon Laser Scanning Microscopy (2PLSM)

Striatonigral (BAC D1) or striatopallidal (BAC D2) neurons expressing soluble eGFP (S. Falcon, R. Gentleman, Bioinformatics 23, 257-8 (2007)) in 275 μm thick corticostriatal slices were identified by somatic eGFP fluorescence. eGFP 2PLSM green signals (500-550 nm) were acquired from eGFP+ BAC Drd2 neurons using 810 nm excitation, while eGFP+ BAC Drd1 neurons required 900 nm excitation. eGFP+ MSNs were then patched using infrared-differential interference contrast (IR-DIC) video microscopy with a Hamamatsu C2400 Newvicon camera/controller system (Hamamatsu, Japan) and a 60X/0.9NA water-dipping lens. Patch electrodes were made by pulling BF150-86-10 glass on a P-97 Flaming/Brown micropipette puller (both from Sutter Instrument Co., Novato, Calif.). The pipette solution contained the following (in mM): 135 KMeSO4 (ICN Biomedicals Inc., Aurora, Ohio), 5 KCl, 10 HEPES, 2 MgATP, 0.2 Na2GTP, and 0.1 spermine, pH=7.25-7.3 with KOH, 270 mOsm/l. As measured in the bath, the pipette resistance was ˜4 MΩ. Seals were formed in voltage-clamp mode on the cell somas with series resistance >1 GΩ. After seal rupture in whole-cell configuration the series-resistance decreased to 10-15 MΩ. Alexa 594 (50 μM, Ca²⁺-insensitive red dye), for visualization of the dendrites, and Fluo-4 (200 μM, Ca²⁺-sensitive green dye), for measuring changes in intracellular dendritic Ca²⁺, was dissolved in the internal pipette solution. eGFP fluorescence was below the threshold for detection in the dendrites and did not contribute background signal during calcium imaging, Thapsigargin was also added to the internal solution in some experiments to block ER-mediated Ca²⁺ release. The thapsigargin was initially dissolved in DMSO at a concentration of 10 mM. This stock was then diluted 1000× in the internal recording solution. Following break in, the internal solution was allowed to approach diffusional equilibrium for at least 15 min prior to imaging. The cells were voltage clamped at −70 mV and monitored for somatic depolarization.

For the duration of the recordings, 2PLSM green and red signals (570-620 nm) were acquired using 810 nm excitation with 90 MHz pulse repetition frequency and ˜250 fs pulse duration at the sample plane. A second puffer pipette containing sphingosine 1-phosphate (S1P, 10 μM) was positioned for focal dendritic application. The S1P was initially dissolved in DMSO at a concentration of 10 mM. This stock was then diluted 1000× into HEPES-buffered ACSF containing 4 mg/ml BSA, pH=7.4. Changes in dendritic Ca²⁺ were measured by acquiring high magnification maximum projection images of dendrite segments 50-100 μm from the soma. These images were acquired with 0.17 μm² pixels and 2.2 μs dwell time and consisted of 10-20 images taken at 0.5 μm focal steps. The change in Ca²⁺ was determined by calculating the percent change in fluorescence of the green dye normalized to the red dye (ΔG/R). Following Ca²⁺ imaging, maximum projection images of the soma and dendritic field were acquired with 0.27 μm² pixels and 2.6 μs pixel dwell time and consisted of ˜80 images taken at 0.7 μm focal steps.

The two-photon excitation source was a Chameleon-XR tunable laser system (705 nm to 980 nm, Coherent Laser Group, Santa Clara, Calif.). Laser average power attenuation was achieved with two Pockels cell electro-optic modulators (models 350-80 and 350-50, Con Optics, Danbury, Conn.). The two cells are aligned in series to provide enhanced modulation range for fine control of the excitation dose (0.1% steps over four decades). The laser-scanned images were acquired with a Bio-Rad Radiance MPD system (Hemel Hempstead, England, UK). The fluorescence emission was collected by external or non-de-scanned photomultiplier tubes (PMTs). The green fluorescence (500 to 550 nm) was detected by a bialkali-cathode PMT and the red fluorescence (570 nm to 620 nm) was collected by a multi-alkali-cathode (S20) PMT. The fluorescence was acquired in 16 bit photon counting mode. The laser light transmitted through the sample was collected by the condenser lens and sent to another PMT to provide a bright-field transmission image in registration with the fluorescent images. The stimulation, display, and analysis software was a custom-written shareware package, WinFluor and PicViewer (John Dempster, Strathclyde University, Glasgow, Scotland; UK).

5) Evaluation of Differential GABA_(A) Receptor Function in D1 and D2 Following Chronic Cocaine Treatment

Cocaine treatment increased the frequency of GABA_(A) receptor miniature inhibitory postsynaptic currents (mIPSCs) in striatonigral neurons as presented in the following figures.

FIG. 31 showed that cocaine treatment increased the frequency of small amplitude GABAergic mIPSCs in BAC D1 striatonigral neurons. (a) Representative spontaneous mIPSCs traces from BAC D1 striatonigral neurons (expressing soluble eGFP under the D1 promoter) taken from mice treated for 15 days with saline or (b) cocaine (20 mg/kg/day). (c) Bar graph summary of mean mIPSC frequency showing a increase in BAC D1 striatonigral neuron mIPSCs frequency following cocaine treatment (Mann-Whitney Rank Sum Test, p<0.05, saline median=0.82 Hz, n=22; cocaine median=1.03 Hz, n=26). (d) Bar graph summary showing that the number of small amplitude mIPSCs (<75 pA) in equal length records (7 min) increased in BAC D1 striatonigral neurons following cocaine treatment (t-test, p<0.05, saline=79.4±8.2, n=22; cocaine=104.2±6.3, n=26). (e) Representative variance-mean current plots from saline treated and cocaine treated BAC D1 neurons suggesting that the cocaine-induced small amplitude events arise from synapses that have fewer GABAA receptors (N) per synapse but receptors with an unchanged unitary receptor conductance (g) (saline N=33, g=31 pS; cocaine N=27, g=30 pS). (f) Representative spontaneous mIPSCs traces from BAC D2 striatopallidal neurons following saline treatment for 15 days and (g) following cocaine treatment for 15 days. (h) Bar graph summary of mean mIPSC frequency in saline and cocaine treated neurons, showing no effect of treatment condition (t-test, p>0.05, saline=0.72±0.05 Hz, n=12; cocaine=0.78±0.07 Hz, n=16). (i) Bar graph summary showing that the number of small amplitude mIPSCs (<75 pA) in equal length records (7 min) was not altered by treatment condition in BAC D2 neurons (t-test, p>0.05, saline=68.1±9.7, n=12; cocaine=71.7±7.5, n=16). (j) Representative variance-mean current plots showing that cocaine treatment did not change in the number of receptors per synapse or the unitary receptor conductance in BAC D2 neurons (saline N=29, g=33 pS; cocaine N=31, g=29 pS).

In summary, FIG. 32 showed that cocaine treatment decreased the number of GABAA channels per synapse in BAC D1 striatonigral neurons. Box plot summaries showing mean mIPSCs kinetics and non-stationary noise analysis measures of individual striatonigral neurons taken from saline-treated control and 15-day treated cocaine BAC D1 mice. (a) Neither mean 10-90% rise times (t-test, p>0.05, Saline=0.64±0.008, n=22; Cocaine=0.65±0.006, n=26) nor (b) mean decay times (ttest, p>0.05, Saline=17.3±0.5, n=22; Cocaine=17.0±0.5, n=26) were different between groups. Non-stationary noise analysis of mIPSCs demonstrated no change between groups in the (c) mean unitary conductance (g) of GABAA receptors of striatonigral neurons (t-test, p>0.05, Saline g=31.1±1.6, n=22; Cocaine g=30.8±1.2, n=26), and a decrease in the (d) mean number of channels per synapse in striatonigral neurons taken from cocaine treated BAC D1 mice (t-test, p<0.05, Saline N=33.1±1.4, n=22; Cocaine N=29.3±1.0, n=26).

In summary, FIG. 33 showed that cocaine treatment did not alter GABAergic mIPSCs kinetics or GABAA receptor number per synapse or unitary receptor conductance in BAC D2 striatopallidal neurons. Box plot summaries showing mean mIPSCs kinetics and non-stationary noise analysis measures of individual striatopallidal neurons taken from saline-treated control and 15-day treated cocaine BAC D2. (a) Neither mean 10-90% rise times (t-test, p>0.05, Saline=0.65±0.007, n=12; Cocaine=0.64±0.010, n=16) nor (b) mean decay times (t-test, p>0.05, Saline=18.0±1.0, n=12; Cocaine=18.0±0.5, n=16) were different between groups. Non-stationary noise analysis of mIPSCs demonstrated no change between groups in the (c) mean unitary conductance (g) of GABAA receptors of striatopallidal neurons (t-test, p>0.05, Saline g=30.7±1.5, n=12; Cocaine g=30.3±1.2, n=16) or in the (d) mean number of channels per synapse in striatopallidal neurons taken from cocaine treated BAC D2 mice (t-test, p>0.05, Saline N=33.8±2.7, n=12; Cocaine N=33.0±1.6, n=16).

Example 14 Molecular Phenotyping and Translational Profiling of Cerebellar Cells

1) Overview

The cerebellum is a region of the brain that plays a role in the integration of sensory perception and motor control. In order to coordinate motor control, there are several neural pathways linking the cerebellum with the cerebral motor cortex (which sends information to the muscles causing them to move) and the spinocerebellar tract (which provides proprioceptive feedback on the position of the body in space). The cerebellum integrates these pathways using constant feedback on body position to fine-tune motor movements (Fine E J, Ionita C C, Lohr L (2002). “The history of the development of the cerebellar examination”. Semin Neurol 22 (4): 375-84. doi:10.1055/s-2002-36759). Studies show that the cerebellum also serves other functions, such as cognitive functions, including attention and the processing of language, music, and other sensory temporal stimuli (Rapp, Brenda (2001). The Handbook of Cognitive Neuropsychology: What Deficits Reveal about the Human Mind. Psychology Press, 481).

Damage to the cerebellum typically is not so debilitating as to cause paralysis, but rather presents as feedback deficits resulting in disorders in fine movement, equilibrium, posture, and motor learning. Ataxia is a complex of symptoms, generally involving a lack of coordination, that is often found in disease processes affecting the cerebellum. To identify cerebellar problems, the neurological examination includes assessment of gait (a broad-based gait being indicative of ataxia), finger-pointing tests and assessment of posture. Structural abnormalities of the cerebellum (hemorrhage, infarction, neoplasm, degeneration) may be identified on cross-sectional imaging. Magnetic resonance imaging is the modality of choice, as computed tomography is insufficiently sensitive for detecting structural abnormalities of the cerebellum (Gilman S (1998). “Imaging the brain. Second of two parts”. N. Engl. J. Med. 338 (13): 889-96).

2) BACarray Mice Expressing eGFP in Individual Cerebellar Cell Types

There are multiple cell layers and several cerebellar cell types. The innermost granular layer contains the cell bodies three types of cells: the numerous and tiny granule cells, the larger Golgi cells and the medium sized neuronal unipolar brush cells. The middle Purkinje layer contains only one type neuron, the Purkinje cell. Purkinje cells are the primary integrative neurons of the cerebellar cortex and provide its sole output. Bergmann glia, a type of glia also known as radial epithelial cells are astrocytes in the cerebellum that have their cell bodies in the Purkinje cell layer and processes that extend into the molecular layer, terminating with bulbous endfeet at the pial surface (Eccles J. C Ito, M and Szentagothai J. (1967). “The cerebellum as a neuronal machine”. Springer Verlag; Llinás, R., Baker, R. and Sotelo, C. (1974). “Electrotonic coupling between neurons in cat inferior olive”. J. Neurophysiol 37: 560-571). The outermost layer of the cerebellar cortex contains two types of inhibitory interneurons: the stellate and basket cells. It also contains the dendritic arbors of Purkinje neurons and parallel fiber tracts from the granule cells. BACarray mice with specific expression patterns in specific cerebellar cell types can be created: expression of tagged ribosomal protein under the control of a regulatory region from the Neurod1 loci to label cerebellar granule cells; expression of tagged ribosomal protein under the control of a regulatory region from the Grm2 loci to label cerebellar inner golgi neurons; expression of tagged ribosomal protein under the control of a regulatory region from the Grp loci to label unipolar brush cells; expression of tagged ribosomal protein under the control of a regulatory region from the Pttg3 loci to label cerebellar astrocytes; and expression of tagged ribosomal protein under the control of a regulatory region from the Lypd6 loci to label stellate, basked cells.

3) Translational Profiling and Molecular Phenotyping of Cells from AT Mice

Ataxia telangiectasia (AT) is a disease whose principle features includes ataxia, development of lymphocytic tumors and cerebellar degeneration (P. J. McKinnon, EMBO Rep 5, 772 (2004); F. Gumy-Pause, P. Wacker, A. P. Sappino, Leukemia 18, 238 (2004); L. Farina et al., J Comput Assist Tomogr 18, 724 (1994); F. Tavani et al., Neuroradiology 45, 315 (2003)). AT is caused by recessive loss of function mutations in the gene encoding ataxia telangiectasia mutated (ATM), a ubiquitously expressed nuclear protein kinase involved in the DNA damage response and cell cycle control (K. Savitsky et al., Science 268, 1749 (1995); R. T. Abraham, Genes Dev 15, 2177 (2001); Y. Shiloh, Nat Rev Cancer 3, 155 (2003); M. F. Lavin, S. Kozlov, Cell Cycle 6, 931 (2007); S. Matsuoka et al., Science 316, 1160 (2007)). Atm knockout mice display many of the characteristics of the human disorder, including sensitivity to irradiation and lymphocytic tumor formation (C. Barlow, K. D. Brown, C. X. Deng, D. A. Tagle, A. Wynshaw-Boris, Nat Genet 17, 453 (1997); K. H. Herzog, M. J. Chong, M. Kapsetaki, J. I. Morgan, P. J. McKinnon, Science 280, 1089 (1998); A. Elson et al., Proc Natl Acad Sci USA 93, 13084 (1996); Y. Xu et al., Genes Dev 10, 2411 (1996); P. R. Borghesani et al., Proc Natl Acad Sci USA 97, 3336 (2000)). However, one of the distinguishing features of AT, the degeneration of cerebellar Purkinje cells, does not occur in the mouse model of the disease. Since clinical evidence of substantial Purkinje cell loss in AT patients is not seen until affected children reach several years of age (R. A. Gatti et al., Clin Rev Allergy Immunol 20, 87 (2001)), and since the average lifespan of a mouse is less than two years, it is possible that the pathogenic process simply does not have enough time to result in Purkinje cell degeneration in the AT mouse model. In one embodiment, ribosomes in specific cerebellar cell types in a mouse model of AT can be molecularly labeled (Doyle et al., submitted).

Molecular phenotyping and translational profiling of Purkinje cells in Atm knockout mice, hereafter Atm −/− mice, could reveal changes in response to the mutation. This enables the identification of genes that are differentially regulated in a cell-type specific manner, by employing the BACarray strategy and TRAP methods. Changes in response to the Atm mutation were analyzed in Purkinje cells and Bergmann glia, a specialized glial cell population with similar abundance and anatomical distribution as Purkinje cells.

Transgenic BACarray mice expressing the eGFP-L10a fusion protein were prepared using the Purkinje cell protein 2 (Pcp2) and Septin 4 (Sept4) BAC vectors described by the GENSAT project (world wide web address: gensat.org; S. Gong et al., Nature 425, 917 (2003)) (FIG. 34). BAC transgenic animals were generated using modified BAC constructs as previously described (J. Zahringer, B. S. Baliga, H. N. Munro, Proc Natl Acad Sci USA 73, 857-61 1976)) using either the Pcp2 gene or the Septin 4 gene as drivers for the ribosomal protein L10 enhanced green fluorescent protein (eGFP L10a) construct in the Purkinje cells, and Bergmann glia, respectively. In other embodiments, other drivers of cell-type specific gene expression could be used, and is in no means meant to be limiting. Transgenic lines were generated and genotyping confirmed using conventional techniques. Pcp2 and Sept4 BACarray transgenic animals were then crossed with Atm +/animals. After two generations, the Pcp2 and Sept4 BACarray constructs could be recovered in Atm +/+ or Atm −/− animals. Heterozygote Atm +/littermates were bred to generate wildtype +/+ and knockout animals. Immunohistochemical and double immunofluorescence staining of sections from the BACarray mice is presented in FIG. 35. Panel A demonstrated the expression of the eGFP-L10a fusion specifically in the soma and primary dendrites of cerebellar Purkinje cells in the Pcp2 line, and panel B demonstrated its expression in Bergmann glia in the Sept4 line.

To ensure that Purkinje cell and Bergmann glial cell mRNAs could be enriched from the Pcp2 and Sept4 BACarray transgenic lines, microarray data were collected from each of the BACarray lines in triplicate by affinity purification of eGFP-L10a containing polysomes. In each case, the levels of expression of immuno-precipitated (IP) mRNAs were compared to their expression levels in the unbound fraction to determine their enrichment in Purkinje cells or Bergmann glia. Scatter plots (FIG. 36) of these data revealed that thousands of mRNAs could be enriched by affinity purification of polysomes from either the Pcp2 or Sept 4 BACarray cerebella. As expected, known Purkinje cell specific mRNAs were specifically enriched in IP samples from the Pcp2 BACarray mice (

Table 22), and known Bergmann glial enriched mRNAs were specifically enriched in the IP samples from the Sept4 BACarray mice (Table 23). The data establish translational profiles and molecular phenotypes of these two cerebellar cell types.

Microarray data were imported into Genespring GX 7.3.1 (Agilent Technologies, Santa Clara, Calif.) as GeneChip CEL files. For each experiment, Atm +/+ and Atm −/− data sets were imported together and normalized using GC RMA. For total cerebellum experiments, four replicates for each condition (Atm +/+ or Atm −/−) were used, for the Septin4 experiments, five replicates for each condition were used, and for the Pcp2 experiments, six replicates were used for each condition. Once imported, experiments were viewed as scatter plots for further analysis. For PCP2 and Septin 4 experiments, Atm +/+ and Atm −/− data were further normalized per chip using the Affymetrix hybridization controls as positive control genes and divided by the constant value of 0.001. In order to generate lists of regulated genes, data were filtered on expression with a cutoff of 25 and filtered on fold change with a cutoff of 1.5. One-way ANOVA analysis was used to determine statistical significance with a p value of ≤0.05.

TABLE 22 Purkinje Cell Positive Control Genes. Fold Change, Fold Change, Gene Symbol GenBank Accession Affymetrix ID PCP2 IP vs. Unb Septin4 IP vs. Unb Slc1a6 NM_009200 1418933_at 14.76 0.126 Kcnip1 NM_027398 1416785_at 12.26 0.627 Pcp4l1 AV337888 1452913_at 11.59 0.959 Car8 BC010773 1424958_at 11.37 0.0251 Cdr2 NM_007672 1417430_at 10.23 0.0651 Trpc3 NM_019510 1417577_at 9.951 0.0976 Kcnip1 NM_027398 1448459_at 9.694 0.456 Pcp2 NM_008790 1419084_a_at 9.553 0.173 Kitl BB815530 1448117_at 9.02 0.599 Kitl M64262 1426152_a_at 7.551 1.116 Plxdc1 AF378760 1424902_at 6.91 0.0552 Kitl BB815530 1415855_at 6.547 1.077 Pcp2 NM_008790 1419085_at 6.21 0.287 Grid2ip NM_133355 1450310_at 5.514 0.414 Calb1 BB246032 1448738_at 4.769 0.0384 Thy1 AV028402 1423135_at 4.488 0.167 Kitl AW551461 1440621_at 3.836 0.985 Dab1 NM_010014 1421100_a_at 3.085 0.346 Car8 X61397 1427482_a_at 2.984 0.0834 Pcp4 NM_008791 1460214_at 2.434 0.642 Dab1 BB644109 1427308_at 2.388 0.151 Calb1 BB246032 1417504_at 1.824 0.0755 Grid2 BG075811 1459245_s_at 1.816 1.032 Kcnip1 AK013657 1454504_at 1.752 1.223 Dab1 BB429377 1435577_at 1.659 0.122 Slc1a6 NM_009200 1418933_at 14.76 0.126 Dab1 BB644109 1427307_a_at 1.529 0.774 Genes known to be specifically expressed in Purkinje cells were analyzed by scatter plot analyses with data from both Pcp2 and Septin4 lines. These genes are enriched in the Pcp2 IP data relative to total cerebellum (Unb), whereas they are underrepresented in the Septin4 IP data relative to total cerebellum (Unb).

TABLE 23 Bergmann Glial Cell Positive Control Genes Fold Change, Fold Change, Gene Symbol GenBank Accession Affymetrix ID Septin4 IPvsUnb PCP2 IPvsUnb Vim AV147875 1456292_a_at 20.41 0.0313 Vim M24849 1450641_at 13.29 0.053 Mlc1 NM_133241 1448139_at 13.07 0.0218 Slc1a3 BB357585 1426340_at 12.85 0.0635 Phgdh L21027 1426657_s_at 12.32 0.106 Vim AV147875 1438118_x_at 12.31 0.0556 Slc1a3 BB357585 1426341_at 11.79 0.268 NM_009115 1419383_at 10.77 0.039 4-Sep AW208509 1455422_x_at 10.48 0.415 Slc1a3 BB357585 1452031_at 9.359 0.0409 Fabp7 NM_021272 1450779_at 8.724 0.0966 Phgdh AV216768 1437621_x_at 8.696 0.0343 4-Sep NM_011129 1448729_a_at 8.277 0.474 Ednrb BF100813 1437347_at 7.807 0.103 Slc1a3 BB308757 1439072_at 7.424 0.537 Slc1a3 BB085413 1443749_x_at 6.165 0.102 Gfap BB183081 1426508_at 6.075 1.031 Gsta4 NM_010357 1416368_at 5.171 0.0499 Ednrb BB451714 1423594_a_at 5.026 0.153 Gfap BB183081 1426509_s_at 4.77 0.408 Gfap BB750040 1440142_s_at 4.311 0.0098 Ednrb BB770914 1426314_at 3.959 0.651 Phgdh BB495884 1456584_x_at 3.039 0.105 Phgdh L21027 1426658_x_at 2.927 0.997 Mthfd1 AV215673 1436704_x_at 2.072 0.663 Phgdhl1 BC024467 1423928_at 2.006 0.576 Phgdhl1 BC024467 1423929_at 1.844 1.363 Nes AI413223 1449022_at 1.794 0.732 Mthfd1 NM_138745 1415917_at 1.786 0.759 Mthfd1 NM_138745 1415916_a_at 1.773 0.32 Genes known to be expressed in Bergmann glial cells were analyzed by scatter plot analyses with data from both Septin4 and Pcp2 lines. These genes are robustly enriched in the Septin4 IP data relative to data from total cerebellum (Unb). Conversely, these genes are underrepresented in data from Pcp2 IP relative to total cerebellum (Unb).

4) Loss of ATM Function

Pcp2 and Sept4 BACarray transgenic animals were then crossed with Atm +/− animals. After two generations, the Pcp2 and Sept4 BACarray constructs could be recovered in Atm +/+ or Atm −/− animals. To determine whether Purkinje cells respond differently than do Bergmann glia to loss of ATM function, microarray data were collected from total cerebellum, from Purkinje cells and from Bergmann glial cells using 6-8 week old Pcp2 and Sept4 wt and Atm −/− BACarray mice. Lists of genes regulated in Atm −/− animals were generated by identifying all genes with expression values greater than 25 and at least a 1.5 fold difference in expression between wt and Atm −/− animals. One-way ANOVA analysis with p-value cutoff of 0.05 was used to select for significance. Scatter plot and Venn diagram analyses were used to identify genes whose expression changed in response to the Atm mutation in whole cerebellum, Purkinje cells or Bergmann glia (FIG. 37). Data for individual genes are presented in Table 24-Table 29.

The majority of genes whose expression is altered in Purkinje cells of Atm −/− mice (65/89) can not be detected by microarray analysis of total cerebellar mRNA. Moreover, only 17 of the 89 mRNAs whose expression changes in Purkinje cells in Atm −/− animals are also changed in Bergmann glial cells. Finally, the levels of expression of 94 genes are altered specifically in Atm −/− Bergmann glia versus Purkinje cells. The responses of specific cell types to the Atm mutation are largely distinct, and that translational profiling and molecular phenotyping of specific cell types using the BACarray strategy can reveal responses that can not be observed by analysis of histologically complex tissues.

TABLE 24 Genes Upregulated in Atm−/− Total Cerebellum Data Gene Symbol GenBank Accession Affymetrix ID Fold Change AI451250 AI451250 1447436_at 21.3 Scn2b AK013326 1430648_at 16.86 Nedd4 NM_010890 1421955_a_at 6.112 2010007H06Rik BB483585 1446748_at 5.587 MGI:2136405 BB272589 1440386_at 3.752 C920006O11Rik BB552195 1435704_at 3.486 Ddx6 BC021452 1424598_at 3.371 Opcml BB383296 1457446_at 2.273 Cyp11a1 C87524 1439947_at 2.113 Btbd15 BC027138 1427271_at 2.048 Mod1 BC011081 1416632_at 2.032 Cgnl1 BB409331 1452309_at 1.941 Loh11cr2a BC004727 1426221_at 1.914 Arcn1 BB757123 1436062_at 1.829 AI835735 AI835735 1459716_at 1.817 Map2k6 BB261602 1426850_a_at 1.783 Nedd4 BG073415 1451109_a_at 1.757 Grid2 NM_008167 1421435_at 1.749 Hif3a AF416641 1425428_at 1.747 Arid5b BB699910 1420973_at 1.747 LOC546041 AI585679 1436740_at 1.744 Thy1 AV028402 1423135_at 1.727 AI593442 BF658761 1444026_at 1.697 Usp2 AI553394 1417168_a_at 1.697 Enpp3 BB039510 1439260_a_at 1.693 Cdkn1a AK007630 1424638_at 1.688 Tspan18 AK011742 1429856_at 1.665 Mecr NM_025297 1417097_at 1.656 D13Ertd787e AU020421 1420098_s_at 1.645 Bcl6 U41465 1421818_at 1.644 Arid5b BB079486 1434283_at 1.64 A730090H04Rik AV251751 1445913_at 1.63 Sgk NM_011361 1416041_at 1.628 1500041N16Rik NM_026399 1418732_s_at 1.602 Dlat AV336908 1426265_x_at 1.589 A430108E01Rik AW541326 1438049_at 1.584 Hif3a BB486740 1446708_at 1.573 Syt13 BE648447 1451045_at 1.564 Mtap6 BM233608 1457316_at 1.555 Cald1 AK014755 1433147_at 1.549 Elmo1 BC024727 1424523_at 1.547 Arf2 AV023312 1438661_a_at 1.544 2610005L07Rik BB471300 1437717_x_at 1.534 AI449310 AW552116 1455451_at 1.523 Scn2b BQ175340 1436134_at 1.522 Mvp NM_080638 1448618_at 1.521 Pcdha11 BB265776 1451769_s_at 1.518 Txnrd3 AI196535 1449623_at 1.516 Frmd6 BC019939 1451264_at 1.513 4732464A07Rik BB004948 1459951_at 1.51 BB627097 1442019_at 1.509 8430436O14Rik AK018466 1452761_a_at 1.508 Genes which are upregulated in Atm−/− cerebellum relative to Atm+/+ cerebellum. Microarray data from Atm+/+ and Atm−/− cerebellum were compared in scatter plots, and filtered on expression of at least 25 and on fold change of at least 1.5, with Atm−/− values greater than Atm+/+. Enriched genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.

TABLE 25 Genes Downregulated in Atm−/− Total Cerebellum Data Gene Symbol GenBank Accession Affymetrix ID Fold Change Tmod3 AK017725 1423088_at 0.66 Leo1 BG065311 1455293_at 0.658 Sirt4 AK016400 1426847_at 0.656 Kcna6 AV328356 1441049_at 0.656 BG066504 1435998_at 0.655 Osbpl6 AI851164 1449627_at 0.654 Cryab AV016515 1434369_a_at 0.653 Malat1 AW012617 1427285_s_at 0.646 Ddx42 AW763628 1443719_x_at 0.642 AV372127 1438754_at 0.639 6430514L14Rik BC025646 1425213_at 0.637 Smarce1 AK018493 1422675_at 0.632 Dnaja4 BQ176119 1434196_at 0.63 1700047I17Rik BB516625 1445334_at 0.629 2010309E21Rik BF017486 1441796_at 0.629 Htr2c BQ174268 1435513_at 0.625 Dpy19l1 BE949451 1433759_at 0.615 Gpr153 AK004377 1426973_at 0.613 Ncam2 AF001287 1425301_at 0.602 2310004I03Rik BM939621 1428698_at 0.598 Ier2 NM_010499 1416442_at 0.597 Cd80 BQ175573 1440592_at 0.591 Vps11 BB322416 1441922_x_at 0.591 1110037N09Rik BG143502 1438216_at 0.588 Gcl BM239632 1438888_at 0.586 Nrip1 BB041915 1454295_at 0.586 Tmod2 AK018223 1430153_at 0.582 Rfxdc2 BB148972 1460567_at 0.577 A130022J15Rik BI149851 1433671_at 0.577 Pou3f2 BE991235 1457734_at 0.567 Tmem25 BB245618 1436644_x_at 0.562 Phf20l1 BB268102 1454939_at 0.556 Mcam NM_023061 1416357_a_at 0.552 Aph1c; Aph1b AK002310 1429466_s_at 0.543 Cryab NM_009964 1416455_a_at 0.541 Fmr1 AF170530 1452550_a_at 0.531 B3galt1 AV328619 1441396_at 0.516 4833427G06Rik AK014780 1430762_at 0.496 Pts NM_011220 1450660_at 0.467 BC027092 BB445523 1435676_at 0.43 BC034204 BB830346 1433661_at 0.425 BC038167 BB211614 1458104_a_at 0.422 Ptch2 BB530125 1457256_x_at 0.415 Dixdc1 BC024834 1425256_a_at 0.415 Fxyd6 AB032010 1417343_at 0.369 Bace1 AF200346 1421825_at 0.356 Pdgfd BB428671 1456532_at 0.34 Lrrc49 BB484412 1447954_at 0.327 Tipin BB796715 1459720_x_at 0.277 C030014I23Rik AK021081 1453044_at 0.265 2310030G06Rik NM_025865 1449357_at 0.238 Rps25 BM729504 1430978_at 0.129 Ppcdc BB329391 1424336_at 0.103 Genes which are downregulated in Atm−/− cerebellum relative to Atm+/+ cerebellum. Microarray data from Atm+/+ and Atm−/− cerebellum were compared in scatter plots, and filtered on expression values of at least 25 and selected for genes with wt values at least 1.5 fold greater than Atm−/− values. Differential genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.

TABLE 26 Genes Upregulated in Atm−/− Septin4 IP Data Gene Symbol GenBank Accession Affymetrix ID Fold Change 2010007H06Rik BB483585 1446748_at 14.16 Nedd4 NM_010890 1421955_a_at 4.926 Bmp5 AV032115 1455851_at 4.228 C920006O11Rik BB552195 1435704_at 3.964 Scn2b AK013326 1430648_at 3.629 AI451250 AI451250 1447436_at 3.451 Ddx6 BC021452 1424598_at 2.672 Bmp5 NM_007555 1421282_at 2.57 Bmp5 NM_007555 1421283_at 2.44 Ccl27 NM_011336 1419188_s_at 2.179 BB520860 1458145_at 2.178 Tle1 NM_011599 1422751_at 1.973 Loh11cr2a BC004727 1426221_at 1.957 Kcna4 BB131475 1438613_at 1.955 Mod1 BC011081 1416632_at 1.942 MGI:2136405 BB272589 1440386_at 1.866 AI467657 1442025_a_at 1.864 Cebpd BB831146 1423233_at 1.846 Btbd15 BC027138 1427271_at 1.777 Tpm1 BM232388 1456623_at 1.767 Wee1 NM_009516 1416773_at 1.621 D15Ertd366e AV114522 1450629_at 1.579 4832420M10 BB012037 1440990_at 1.57 Hhip NM_020259 1421426_at 1.547 4930466F19Rik AV044111 1432648_at 1.541 Il11ra1; Il11ra2 BC004619 1417505_s_at 1.52 MGI:1921571 AK011357 1426612_at 1.512 Genes which are upregulated in Atm−/− relative to Atm+/+ in the Septin4 experiments. Microarray data from Atm+/+ and Atm−/− Septin4 IPs were compared in scatter plots, and filtered on expression of at least 25 and with Atm−/− values at least 1.5 fold greater than Atm+/+. Enriched genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.

TABLE 27 Genes Downregulated in Atm−/− Septin4 Data Gene Symbol GenBank Accession Affymetrix ID Fold Change Aak1 AW121504 1441782_at 0.667 AI503515 1437979_at 0.666 Nmnat1 AF260924 1425773_s_at 0.663 BM940493 1445699_at 0.663 Dpy19l1 BE949451 1433759_at 0.663 Jmjd3 BB494168 1447612_x_at 0.662 Zfyve20 BC017622 1429517_at 0.662 2410076I21Rik AV216663 1441957_x_at 0.66 Tln2 AV270892 1429111_at 0.657 Hdlbp C77256 1449615_s_at 0.657 Fbxo5 AK011820 1429499_at 0.657 Ddc AF071068 1426215_at 0.656 Hmg20a AI987819 1428920_at 0.649 Pcdhb12 NM_053137 1422877_at 0.649 Zcchc3 AV140894 1428401_at 0.647 Vps11 AK004695 1428110_x_at 0.647 Itsn1 AA172344 1452338_s_at 0.646 LOC545039 BB099075 1440815_x_at 0.646 2810452K22Rik AK007812 1454253_at 0.644 Zdhhc24 BB271151 1428278_at 0.644 BB354954 1444359_at 0.644 Sstr3 BQ174132 1441603_at 0.641 4933427G17Rik AK016951 1431905_s_at 0.638 Calml4 AY061807 1424713_at 0.637 Tbl3 BC019504 1415750_at 0.636 A830053O21Rik BB271275 1446501_at 0.635 Car7 BB193643 1443824_s_at 0.634 Zfp316 AV367169 1444528_at 0.63 4833424O15Rik BB346520 1429249_at 0.629 2310076O21Rik BC017618 1451304_at 0.627 Dusp16 BB121278 1440615_at 0.627 Tgoln1 AI314055 1423308_at 0.625 Sv2a BC026494 1423406_at 0.625 Map3k10 BG070411 1440500_at 0.624 Foxp1 BM220880 1435221_at 0.623 Bace1 AF200346 1421825_at 0.623 Nr2f2 AI527205 1436475_at 0.619 AV028487 1457337_at 0.617 1110025F24Rik AK012340 1453562_a_at 0.617 AV023306 1438395_at 0.617 8430427H17Rik AK018446 1428284_at 0.616 Cops3 BB283617 1443570_at 0.616 4930506M07Rik BB559293 1429055_at 0.614 Larp6 NM_026235 1418400_at 0.609 Lzp-s AV058500 1439426_x_at 0.609 BQ175164 1441091_at 0.607 C230004F18Rik BB380166 1445854_at 0.607 Doc2b BM117900 1420667_at 0.606 Atm AK021102 1428830_at 0.605 A930001A20Rik BB280276 1431522_at 0.603 Pim1 AI323550 1435458_at 0.602 Susd4 BF455403 1460593_at 0.598 BB384728 1447012_at 0.598 4930538K18Rik BB548441 1453632_at 0.596 Csf1r AI323359 1419872_at 0.594 Fhad1 AW537651 1439190_at 0.59 MGI:1925112 BF719766 1438769_a_at 0.583 Cd276 NM_133983 1417599_at 0.578 Cpm AK004327 1453009_at 0.574 1110059F07Rik BE956260 1447533_at 0.573 Pts NM_011220 1450660_at 0.567 Prss18 NM_011177 1448982_at 0.559 BC027092 BB445523 1435676_at 0.556 Cryab NM_009964 1416455_a_at 0.536 H2-DMb1 NM_010387 1418638_at 0.534 Tmem25 BB245618 1436644_x_at 0.533 Dixdc1 BB758432 1435207_at 0.532 D630044F24Rik AV285979 1454839_a_at 0.528 Sox10 BC018551 1451689_a_at 0.525 Dnaja4 BQ176119 1434196_at 0.518 Rfxdc2 BB148972 1460567_at 0.506 3110005L24Rik AA611589 1431381_at 0.504 Lzp-s AV066625 1436996_x_at 0.498 Islr NM_012043 1418450_at 0.486 Pex2 AV327933 1451062_a_at 0.475 Lemd1 AV156411 1445642_at 0.472 Nrp2 BB752129 1435349_at 0.451 B230220N19Rik BB539872 1442585_at 0.445 Fos AV026617 1423100_at 0.42 BB047533 1446771_at 0.412 AI836671 1459747_at 0.394 Mcam NM_023061 1416357_a_at 0.384 Rab27a BB025283 1429123_at 0.326 4921517L17Rik BC027204 1425047_a_at 0.31 Rps25 BM729504 1430978_at 0.308 Dixdc1 BC024834 1425256_a_at 0.289 C030014I23Rik AK021081 1453044_at 0.287 Ppcdc BB329391 1424336_at 0.244 Tipin BB796715 1459720_x_at 0.199 Genes which are downregulated in Atm−/− relative to Atm+/+ in the Septin4 experiments. Microarray data from Atm+/+ and Atm−/− Septin4 IPs were compared in scatter plots, and filtered on expression values of at least 25 and selected for genes with Atm+/+ values at least 1.5 fold greater than Atm−/− values. Differential genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.

TABLE 28 Genes Upregulated in Atm−/− Pcp2 Data Gene Symbol GenBank Accession Affymetrix ID Fold Change Nedd4 NM_010890 1421955_a_at 4.614 MGI:2136405 BB272589 1440386_at 4.155 AI451250 AI451250 1447436_at 4.04 Eva1 BC015076 1416236_a_at 3.992 Tex12 AF285582 1421183_at 3.409 C920006O11Rik BB552195 1435704_at 2.684 AI593442 AV327193 1434052_at 2.584 Opcml BB383296 1457446_at 2.538 Ddx6 BC021452 1424598_at 2.372 2900052N01Rik AU067665 1436231_at 2.167 Cyp11a1 C87524 1439947_at 1.983 9330159M07Rik BB214614 1441092_at 1.973 U58494 1451626_x_at 1.962 Btbd15 BC027138 1427271_at 1.948 Mod1 BC011081 1416632_at 1.891 Anln BI690018 1433543_at 1.882 Nedd4 BG073415 1451109_a_at 1.869 E430024C06Rik AU018141 1453238_s_at 1.853 AI593442 BF658761 1444026_at 1.834 Pml BB667149 1456103_at 1.824 Pml NM_008884 1448757_at 1.777 Ccrn4l NM_010490 1448715_x_at 1.721 AI835735 AI835735 1459716_at 1.703 Pvrl3 NM_021495 1448673_at 1.698 C330006P03Rik BB398124 1436387_at 1.681 5730601F06Rik BI110339 1431206_at 1.671 Zfp191 BB579760 1426896_at 1.648 Rora BB306272 1436326_at 1.644 Ccrn4l AI987693 1455316_x_at 1.571 Zfp451 BI714076 1456350_at 1.556 Tpm1 AK002271 1423049_a_at 1.542 Peg3 AB003040 1417355_at 1.523 Chst2 BB770422 1442234_at 1.519 C230082I21Rik AV317838 1455146_at 1.519 Ddx26 BB381966 1423275_at 1.509 Mjd BQ176512 1434408_at 1.508 C79468 BQ086025 1459900_at 1.508 Mtf2 BG066919 1449115_at 1.507 Genes which are upregulated in Atm−/− relative to Atm+/+ in the Pcp2 experiments. Data from Atm+/+ and Atm−/− Pcp2 IPs were compared in scatter plots, and filtered on expression of at least 25 and with Atm−/− values at least 1.5 fold greater than Atm+/+ values. Enriched genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.

TABLE 29 Genes Downregulated in Atm−/− Pcp2 Data Gene Symbol GenBank Accession Affymetrix ID Fold Change Nlgn3 BB308872 1456384_at 0.662 Ptbp1; LOC236294 BM195499 1458284_at 0.661 Fth1; Dpep2 AK002778 1427021_s_at 0.66 Zfyve21 BC019521 1424669_at 0.656 Gtf2f1 AV325174 1417699_at 0.655 1700027J05Rik BB195788 1438638_x_at 0.653 MGI:1925112 BF719766 1438769_a_at 0.653 Tm2d1 AF353993 1451996_at 0.64 BB174864 1435126_at 0.64 Sh2d3c AB043953 1415886_at 0.64 Ddx46 BG243238 1457744_at 0.639 Spa17 NM_011449 1417635_at 0.633 Boc BB005556 1426869_at 0.626 4930549C01Rik NM_026300 1421367_at 0.625 BQ175377 1443960_at 0.624 Adamts4 BB443585 1452595_at 0.62 Cryab AV016515 1434369_a_at 0.619 BC027092 BB445523 1435676_at 0.617 D730049H07Rik BQ173909 1424989_at 0.616 Fbf1 AF241249 1425979_a_at 0.615 AA589481 BE200208 1437276_at 0.615 Pts NM_011220 1450660_at 0.614 Cyp4f13 NM_130882 1418767_at 0.613 BC020025 BC020025 1451298_at 0.607 AV340375 AV273541 1437737_at 0.604 Mmp17 NM_011846 1448598_at 0.596 Pcdh8 BB076893 1447825_x_at 0.588 Tcrb-V13 X14388 1427656_at 0.579 Cd4 U75219 1427779_a_at 0.575 Atm AK021102 1428830_at 0.57 Decr2 BE952632 1423495_at 0.567 Tmod2 AK018223 1430153_at 0.566 Dusp7 BE136125 1452097_a_at 0.558 1200003M09Rik BC011077 1423887_a_at 0.551 Dap BC024876 1423790_at 0.546 2310004I03Rik BM939621 1428698_at 0.543 Rps25 BM729504 1430978_at 0.513 BG066504 1435998_at 0.512 Rab3il1 AK004767 1428391_at 0.507 Tmem25 BB245618 1436644_x_at 0.481 C330003B14Rik AU019881 1419958_at 0.469 Fmo2 NM_018881 1422904_at 0.436 BB393620 1443754_x_at 0.423 Dixdc1 BC024834 1425256_a_at 0.392 4930523C11Rik; AV255799 1436129_at 0.387 Adam6 Bace1 AF200346 1421825_at 0.379 Lrrc49 BB484412 1447954_at 0.314 BC003855 1425469_a_at 0.313 2310030G06Rik NM_025865 1449357_at 0.274 Tipin BB796715 1459720_x_at 0.227 Ppcdc BB329391 1424336_at 0.0711 Genes which are downregulated in Atm−/− relative to Atm+/+ in the Pcp2 experiments. Microarray data from Atm+/+ and Atm−/− Pcp2 IPs were compared in scatter plots, and filtered on expression values of at least 25 and selected for genes with Atm+/+ values at least 1.5 fold greater than Atm−/− values. Differential genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05. Identification of Functional Groups of Genes

To identify functional groups of genes which are enriched in the knockout cerebellum and each cell type, Gene Ontology (GO) analysis was performed using both DAVID (G. Dennis, Jr. et al., Genome Biol 4, P3 (2003)) and Genespring, each program essentially giving the same results. It was also essential to compare the gene lists and pertinent literature in order to completely analyze data as the GO database is incomplete. Genes enriched in total cerebellum of Atm −/− animals include those involved in cell cycle control (Tacc1, Cdkn1a (p21)) and apoptosis (Cdkn1a (p21)). Bergmann glial cells from Atm −/− animals are enriched in genes related to development and cellular differentiation, including BMP5. Purkinje cells from Atm −/− animals are enriched in genes involved in chromatin organization, nucleotide metabolism, and transcription regulation. These include transcription factors such as Tex12, Zfp191, Rora, Mtf2, and Mjd and genes involved in mRNA metabolism such as Ccrn41 and Ddx6. Several of these differentially regulated genes are either directly or indirectly associated with ataxia. Rora is an orphan retinoic acid receptor which is mutated in the staggerer mouse mutant (B. A. Hamilton et al., Nature 379, 736 (1996)). Mjd (Atxn3, Sca3) is the gene responsible for Machado-Joseph disease (Y. Kawaguchi et al., Nat Genet 8, 221 (1994)). Ddx6 is known to associate with Ataxin-2 in P-bodies, multiprotein complexes involved in mRNA degradation (U. Nonhoff et al., Mol Biol Cell 18, 1385 (2007); N. Cougot, S. Babajko, B. Seraphin, J Cell Biol 165, 31 (2004)). Ccrn41 is a deadenylase which is also found in P-bodies (N. Cougot, S. Babajko, B. Seraphin, J Cell Biol 165, 31 (2004)); deadenylation is a critical first step in the degradation of mRNA by P-bodies. Over-expression of these genes could potentially either enhance neurodegeneration of Purkinje cells, or protect against it.

Example 15 Screenint Methods for Parkinson's Disease

1) Translational Profiles of VTA and SNc Neurons

Dopamine producing (dopaminergic) neurons are present primarily in the ventral tegmental area (VTA) of the midbrain, substantia nigra pars compacta (SNc), and the arcuate nucleus of the hypothalamus Dopaminergic neurons of the SNc are particularly susceptible to neurodegeneration in Parkinson's disease (PD) and in pharmacologically-induced models of PD. The selective degeneration of the dopaminergic neurons in the substantia nigra pars compacta leads to PD but the exact cause for this nigral cell loss is still unknown. The ventral tegmental area dopaminergic neurons are relatively spared in comparison. The tremor, bradykinesia and rigidity seen in PD patients are in a large part caused by the loss of striatal dopamine innervation. Expression of a ribosomal protein (e.g. L10a) fused to a detectable tag, whose expression is regulated by SNc and VTA-specific gene regulatory regions can be used to explore the differential vulnerability of SNc cells and VTA cells in animal models of PD or subjects diagnosed with PD or Parkinsonian-like symptoms. By utilizing the BACarray and TRAP methods described herein, mice with molecularly tagged ribosomal proteins are produced. These are driven by regulatory sequences for cell-type specific expression in either the SNc or VTA (FIG. 38). It is expected that differentially expressed mRNAs in SNc cells will be identified as potential therapeutic targets for the amelioration of PD.

2) Translational Profiles of VTA and SNc Neurons in PD

Dopaminergic cell loss mimicking what is seen in PD is induced with intracerebral injections of the neurotoxins 6-hydroxydopamine (6-OHDA) or 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) either bilaterally or unilaterally in rodents (either mice or rats). By utilizing the BACarray and TRAP methods described herein, mice with molecularly tagged ribosomal proteins are produced. These are driven by regulatory sequences for cell-type specific expression in either the SNc or VTA (FIG. 39). Three weeks following a 6-OHDA treatment (bilateral or unilateral treatment), gene translational profiles from the SNc and VTA neurons are obtained. These translational profiles are compared to reference profiles from mice treated with saline vehicle. It is expected that therapeutic targets relevant to PD will be obtained (FIG. 40).

3) Screening for Therapeutic Targets in the Striatopallidal Cells for Antagonism or Stimulation

_High-frequency stimulation of the subthalamic nucleus (STN) is one neurosurgical procedure currently available for the alleviation of motor symptoms of PD and the debilitating medication-induced dyskinesias that often accompany the disease. Stimulation of the STN is achieved by implanting deep brain stimulation (DBS) electrodes precisely in the nucleus. The striatopallidal cells of the striatum tonically inhibit the Globus Pallidus External segment (GPe) and the Sub-thalamic nucleus (STN) (FIG. 41). By inhibiting activity in striatopallidal cells with a small molecule or other non-electrical, non-surgery requiring method, it will be possible to alleviate the tonically inhibited STN neurons and alleviate PD symptoms, much in the way of deep brain stimulation. By utilizing the methods described herein and exemplified, using D2 BACarray mice one mRNA was found to be selectively enriched in striatopallidal neurons of the striatum is Gpr6 (Table 12). Gpr6 represents a target for which antagonists can be screened for and developed as a therapeutic for PD. In a similar manner, any mRNA found to be downregulated in striatopallidal cells represents a target for agonism as a therapeutic for PD.

Example 16 Screenint Methods for Obesity

The hypothalamus plays a central role in several regulatory and homeostatic processes including blood pressure, body temperature, sleep, fluid and electrolyte balance, and body weight. The lateral hypothalamus in particular is implicated as a feeding and satiety center. Food intake is regulated by hypothalamic neuropeptides which respond to peripheral signals. Homeostasis disrupted can lead to feeding and metabolic disorders leading to such diseases as obesity. Orexin, also named Hypocretin, is a excitatory neuropeptide hormone released by the lateral hypothalamus Orexin release modulates feeding behavior, wakefulness, and energy expenditure. A hypothalamic Hcrt (orexin) BACarray line has expression in the lateral hypothalamus

By utilizing the BACarray and TRAP methods described herein, mice with molecularly tagged ribosomal proteins are produced. These are driven by regulatory sequences for lateral hypothalamic cell-type specific expression under the control of regulatory sequences from the Hcrt (orexin) locus. By obtaining translational profiles from these animals, it is expected that targets relevant to obesity will be identified, in particular for feeding regulation.

While some embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby. 

What is claimed is:
 1. A method for producing a composition comprising isolated complexes that comprise one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to a fluorescent tag, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering the agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; and (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs, thereby producing a composition comprising isolated complexes that comprise one or more mRNAs translated in the specific subpopulation of cells, wherein the regulatory region comprises regulatory sequences from a dopamine D1 receptor (Drd1a) or a dopamine D2 receptor (Drd2) locus.
 2. A method for producing a composition comprising isolated complexes that comprise one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to a fluorescent tag, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering the agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; and (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs, thereby producing a composition comprising isolated complexes that comprise one or more mRNAs translated in the specific subpopulation of cells, wherein the regulatory region causes expression in a neuron, the neuron is associated with a disease or disorder, and the disease or disorder is Parkinson's disease, addiction, attention deficit hyperactivity disorder, or Huntington's disease.
 3. A method for producing a composition comprising isolated complexes that comprise one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to a fluorescent tag, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering the agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; and (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs, thereby producing a composition comprising isolated complexes that comprise one or more mRNAs translated in the specific subpopulation of, wherein the regulatory region causes expression in Purkinje cells, granule cells, golgi neurons, or unipolar brush cells.
 4. A method for producing a composition comprising isolated complexes that comprise one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to a fluorescent tag, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering the agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; and (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs, thereby producing a composition comprising isolated complexes that comprise one or more mRNAs translated in the specific subpopulation of cells, wherein the regulatory region comprises a regulatory sequence of a Purkinje Cell Protein-2 (Pcp2), Neurogenic Differentiation 1 (NeuroD1), Glutamate Receptor Metabotropic 2 (Grm2), Gastrin-Releasing Peptide (Grp), Septin 4 (Sept4), or Aldehyde Dehydrogenase I Family Member LI (Aldh1l1) locus.
 5. A method for producing a composition comprising isolated complexes that comprise one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to a fluorescent tag, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering the agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; and (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs, thereby producing a composition comprising isolated complexes that comprise one or more mRNAs translated in the specific subpopulation of cells, wherein the regulatory region comprises a regulatory sequence of a CKLF-like MARVEL Transmembrane Domain Containing 5 (Cmbm5), Cholecystokinin (Cck), Choline Acetyltransferase (Chat), Cortistatin (Cort), Glycosyltransferase 25 Domain-Containing 2 (G1t25d2), Lymphocyte Antigen 6 (LY6)/Plasminogen Activator, Urokinase Receptor (PLAUR) Domain Containing 6 (Lypd6), Neurotensin Receptor 1 (Ntsr1), Oligodendrocyte Transcription Factor 2 (Olig2), Prepronociceptin (Pnoc), or E Twenty-Six (Ets) 1 Variant Gene 1 (Etv1) locus.
 6. A method for producing a composition comprising isolated complexes that comprise one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to a fluorescent tag, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering the agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; and (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs, thereby producing a composition comprising isolated complexes that comprise one or more mRNAs translated in the specific subpopulation of cells, wherein the nucleotide sequences of the mRNAs are, or include, nucleotide sequences of mRNAs expressed by neurons, and the neurons are striatonigral neurons or striatopallidal neurons.
 7. A method for producing a composition comprising isolated complexes that comprise one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to a fluorescent tag, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering the agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; and (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs, thereby producing a composition comprising isolated complexes that comprise one or more mRNAs translated in the specific subpopulation of cells, wherein the regulatory region causes expression in a neuron, the nucleotide sequences of the mRNAs are, or include, nucleotide sequences of mRNAs expressed by neurons, and the neurons are Purkinje cells, granule cells, golgi neurons, or unipolar brush cells.
 8. A method for producing a composition comprising isolated complexes that comprise one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to a fluorescent tag, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering the agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; and (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs, thereby producing a composition comprising isolated complexes that comprise one or more mRNAs translated in the specific subpopulation of cells, wherein the regulatory region causes expression in Bergmann glial cells or astrocytes.
 9. The method of claim 8, wherein the nucleotide sequences of the mRNAs are, or include, nucleotide sequences of mRNAs expressed by Bergmann glial cells or astrocytes.
 10. A method for determining a nucleotide sequence of one or more messenger ribonucleic acid molecules (mRNAs) translated in a specific subpopulation of cells of an organism after delivery of an agent to the organism to identify agents having a reference translational profile, the method comprising: (a) providing an organism that expresses a ribosomal fusion protein in the subpopulation of cells of the organism, wherein the ribosomal fusion protein comprises ribosomal protein L10a fused to an optically detectable protein, and wherein the ribosomal fusion protein is encoded by a nucleotide sequence operably linked to a regulatory region that causes expression of the ribosomal fusion protein in the subpopulation of cells; (b) delivering a reference agent to the organism comprising the subpopulation of cells expressing the ribosomal fusion protein; (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs; (d) determining a nucleotide sequence of one or more of the mRNAs, thereby producing a reference translational profile; (e) repeating steps (a) through (d) for one or more additional agents, thereby producing one or more additional profiles; and (f) comparing the one or more additional profiles to the reference translational profile to identify one or more additional agents having the reference translational profile.
 11. A method for determining a nucleotide sequence of one or more messenger ribonucleic acid molecules (mRNAs) translated in a cell type of a mouse after delivery of an agent to the mouse, the method comprising: (a) providing a mouse comprising a nucleic acid molecule comprising: a nucleotide sequence encoding a ribosomal fusion protein comprising mouse ribosomal protein L10a having the amino acid sequence of SEQ ID NO: 30 fused to an optically detectable protein, wherein the nucleotide sequence is operably linked to an endogenous mouse regulatory sequence that causes expression of the fusion protein in a chosen cell type, and wherein the ribosomal fusion protein is incorporated in an intact ribosome that translates and/or binds mRNA; (b) delivering the agent to the mouse expressing the ribosomal fusion protein; (c) isolating from the organism complexes comprising the ribosomal fusion protein associated with mRNAs; and (d) determining a nucleotide sequence of one or more of the mRNAs.
 12. The method of claim 10 or claim 11, wherein the regulatory region causes expression in a neuron.
 13. The method of claim 10 or claim 11, wherein the regulatory region causes expression in a brain neuron.
 14. The method of claim 10 or claim 11, wherein the regulatory region causes expression in a striatonigral neuron or a striatopallidal neuron.
 15. The method of claim 10 or claim 11, wherein the regulatory region causes expression in a brain stem motor neuron, a spinal cord motor neurons, or an upper motor neurons.
 16. The method of claim 10 or claim 11, wherein the regulatory region causes expression in a striatal, hippocampal, cortical, cerebellar, spinal cord, hypothalamic, pineal, retinal, auditory, olfactory, brain stem, or vestibular neuron.
 17. The method of claim 10 or claim 11, wherein the regulatory region causes expression in Purkinje cells, granule cells, golgi neurons, or unipolar brush cells.
 18. The method of claim 10 or claim 11, wherein the regulatory region causes expression in Bergmann glial cells or astrocytes.
 19. The method of claim 10 or claim 11, wherein the regulatory region comprises regulatory sequences from a dopamine D1 receptor (Drd1a) or a dopamine D2 receptor (Drd2) locus.
 20. The method of claim 10 or claim 11, wherein the regulatory region comprises a regulatory sequence of a Purkinje Cell Protein-2 (Pcp2), Neurogenic Differentiation 1 (NeuroD1), Glutamate Receptor Metabotropic 2 (Grm2), Gastrin-Releasing Peptide (Grp), Septin 4 (Sept4), or Aldehyde Dehydrogenase I Family Member LI (Aldh1l1) locus.
 21. The method of claim 10 or claim 11, wherein the regulatory region comprises a regulatory sequence of a CKLF-like MARVEL Transmembrane Domain Containing 5 (Cmbm5), Cholecystokinin (Cck), Choline Acetyltransferase (Chat), Cortistatin (Cort), Glycosyltransferase 25 Domain-Containing 2 (Glt25d2), Lymphocyte Antigen 6 (LY6)/Plasminogen Activator, Urokinase Receptor (PLAUR) Domain Containing 6 (Lypd6), Neurotensin Receptor 1 (Ntsr1), Oligodendrocyte Transcription Factor 2 (Olig2), Prepronociceptin (Pnoc), or E Twenty-Six (Ets) 1 Variant Gene 1 (Etv1) locus.
 22. The method of claim 12, wherein the neuron is associated with a disease or disorder.
 23. The method of claim 22, wherein the disease or disorder is Parkinson's disease, addiction, attention deficit hyperactivity disorder, or Huntington's disease.
 24. The method of claim 10 or claim 11, wherein the agent is a small molecule, a drug, an antibody, a hybrid antibody, an antibody fragment, a siRNA, an antisense RNA, an aptamer, a protein, or a peptide.
 25. The method of claim 12, wherein the nucleotide sequences of the mRNAs are, or include, nucleotide sequences of mRNAs expressed by the neuron.
 26. The method of claim 25, wherein the neuron is a brain neuron.
 27. The method of claim 25, wherein the neuron is a striatonigral neuron or striatopallidal neuron.
 28. The method of claim 25, wherein the neuron is a brain stem motor neuron, spinal cord motor neuron, or upper motor neuron.
 29. The method of claim 25, wherein the neuron is a striatal, hippocampal, cortical, cerebellar, spinal cord, hypothalamic, pineal, retinal, auditory, olfactory, brain stem, or vestibular neuron.
 30. The method of claim 25, wherein the neuron is a Purkinje cell, granule cell, golgi neuron, or unipolar brush cells.
 31. The method of claim 10 or claim 11, wherein the nucleotide sequences of the mRNAs are, or include, nucleotide sequences of mRNAs expressed by Bergmann glial cells or astrocytes.
 32. The method of claim 10 or claim 11, wherein step (d) further comprises quantifying one or more mRNAs. 